Gene Symbol: rbsB
Description: D-ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor
Alias: ECK3745, JW3730, prlB, rbsP
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Ahn T, Kin H. Differential effect of precursor ribose binding protein of Escherichia coli and its signal peptide on the SecA penetration of lipid bilayer. J Biol Chem. 1996;271:12372-9 pubmed
    ..When the pRBP and ATP were present together, however, the penetration of SecA increased dramatically underlining the importance of the SecY/E complex for the membrane insertion of SecA. ..
  2. Björkman A, Mowbray S. Multiple open forms of ribose-binding protein trace the path of its conformational change. J Mol Biol. 1998;279:651-64 pubmed
    ..It seems certain that the conformational path that links the forms described here is that followed during ligand retrieval, and in ligand release into the membrane-bound permease system. ..
  3. Mowbray S, Cole L. 1.7 A X-ray structure of the periplasmic ribose receptor from Escherichia coli. J Mol Biol. 1992;225:155-75 pubmed
  4. Björkman A, Binnie R, Zhang H, Cole L, Hermodson M, Mowbray S. Probing protein-protein interactions. The ribose-binding protein in bacterial transport and chemotaxis. J Biol Chem. 1994;269:30206-11 pubmed
    ..2-A resolution) showed that the changes in local structure were accompanied by a diffuse pattern of structural changes in the surrounding region, implying that the suppression derives from a combination of sources. ..
  5. Binnie R, Zhang H, Mowbray S, Hermodson M. Functional mapping of the surface of Escherichia coli ribose-binding protein: mutations that affect chemotaxis and transport. Protein Sci. 1992;1:1642-51 pubmed
    ..A portion of the area involved in transport is also essential to chemotactic function. On the opposite face of the protein, mutations in residues near the hinge are shown to affect chemotaxis specifically. ..
  6. Li H, Cao Z, Zhao L, Wang J. Analysis of conformational motions and residue fluctuations for Escherichia coli ribose-binding protein revealed with elastic network models. Int J Mol Sci. 2013;14:10552-69 pubmed publisher
    ..The results directly indicate that the dominant dynamic characteristics of protein structures can be captured from their static native state using coarse-grained models. ..
  7. Iida A, Harayama S, Iino T, Hazelbauer G. Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12. J Bacteriol. 1984;158:674-82 pubmed
    ..the same position as previously identified mutations causing defects in ribokinase ( rbsK ) or ribose transport ( rbsP )...
  8. Bell A, Buckel S, Groarke J, Hope J, Kingsley D, Hermodson M. The nucleotide sequences of the rbsD, rbsA, and rbsC genes of Escherichia coli K12. J Biol Chem. 1986;261:7652-8 pubmed
    ..components of the high affinity ribose transport system in Escherichia coli, and together with the sequences of rbsB (Groarke, J.M., Mahoney, W.C., Hope, J.N., Furlong, C.E., Robb, F.T., Zalkin, H., and Hermodson, M.A. (1983) J...
  9. Zheng C, Yang L, Hoopmann M, Eng J, Tang X, Weisbrod C, et al. Cross-linking measurements of in vivo protein complex topologies. Mol Cell Proteomics. 2011;10:M110.006841 pubmed publisher
    ..Furthermore, our unbiased data provide novel in vivo topological information that can impact understanding of biological function, even for cases where high resolution structures are not yet available. ..

More Information


  1. Park Y, Park C. Topology of RbsC, a membrane component of the ribose transporter, belonging to the AraH superfamily. J Bacteriol. 1999;181:1039-42 pubmed
    ..This result is consistent with the model, based on the results of alkaline phosphatase fusions, in which the protein traverses the membrane six times and the N and C termini are exposed to the cytoplasm...
  2. Hope J, Bell A, Hermodson M, Groarke J. Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase. J Biol Chem. 1986;261:7663-8 pubmed
    ..It includes the 3' terminus of rbsB (the gene for ribose-binding protein) and the entire rbsK gene, encoding ribokinase...
  3. Emr S, Hanley Way S, Silhavy T. Suppressor mutations that restore export of a protein with a defective signal sequence. Cell. 1981;23:79-88 pubmed
    ..These results suggest that ribosomes play an important role in the export of lambda receptor to the outer membrane. ..
  4. Song T, Park C. Effect of folding on the export of ribose-binding protein studied with the genetically isolated suppressors for the signal sequence mutation. J Mol Biol. 1995;253:304-12 pubmed
    ..Most of them are concentrated in one of the subdomains, suggesting that the folding event in the N-domain of RBP is crucial in the rate-determining step. ..
  5. Galloway D, Furlong C. The role of ribose-binding protein in transport and chemotaxis in Escherichia coli K12. Arch Biochem Biophys. 1977;184:496-504 pubmed
  6. Wick L, Quadroni M, Egli T. Short- and long-term changes in proteome composition and kinetic properties in a culture of Escherichia coli during transition from glucose-excess to glucose-limited growth conditions in continuous culture and vice versa. Environ Microbiol. 2001;3:588-99 pubmed
    ..adaptation to glucose-limitation was achieved by upregulation of 12 proteins, namely MglB, MalE, ArgT, DppA, RbsB, YdcS, LivJ (precursor), UgpB (precursor), AceA, AldA, AtpA and GatY...
  7. Kim I, Kim E, Yoo S, Shin D, Min B, Song J, et al. Ribose utilization with an excess of mutarotase causes cell death due to accumulation of methylglyoxal. J Bacteriol. 2004;186:7229-35 pubmed
    ..It was also demonstrated that a small amount of MG is synthesized by monoamine oxidase. ..
  8. Beloin C, Valle J, Latour Lambert P, Faure P, Kzreminski M, Balestrino D, et al. Global impact of mature biofilm lifestyle on Escherichia coli K-12 gene expression. Mol Microbiol. 2004;51:659-74 pubmed
    ..These results constitute a comprehensive analysis of the global transcriptional response triggered in mature E. coli biofilms and provide insights into its physiological signature. ..
  9. Groarke J, Mahoney W, Hope J, Furlong C, Robb F, Zalkin H, et al. The amino acid sequence of D-ribose-binding protein from Escherichia coli K12. J Biol Chem. 1983;258:12952-6 pubmed
    ..A signal peptide sequence of 23 or 25 residues was also deduced from the DNA sequence. It shows the characteristic features of prokaryotic signal peptides. ..
  10. Ahn T, Ko J, Cho E, Yun C. Conformational change of Escherichia coli signal recognition particle Ffh is affected by the functionality of signal peptides of ribose-binding protein. Mol Cells. 2009;27:681-7 pubmed publisher
    ..However, MT had marginal effect on these conformational changes suggesting that the in vivo functionality of signal peptide is important in the interaction with Ffh and concomitant structural change of the protein. ..
  11. Allert M, Dwyer M, Hellinga H. Local encoding of computationally designed enzyme activity. J Mol Biol. 2007;366:945-53 pubmed
  12. Lager I, Fehr M, Frommer W, Lalonde S. Development of a fluorescent nanosensor for ribose. FEBS Lett. 2003;553:85-9 pubmed
    ..Inhibitor studies suggest that uptake is mediated by a monosaccharide transporter of the GLUT family, however, ribose taken up into the cell was not or only slowly released, indicating irreversibility of uptake. ..
  13. Pradel N, Santini C, Ye C, Fevat L, Gérard F, Alami M, et al. Influence of tat mutations on the ribose-binding protein translocation in Escherichia coli. Biochem Biophys Res Commun. 2003;306:786-91 pubmed
    ..These results would suggest that under the Sec-defective conditions the export of a portion of folded RBP could be rescued by the Tat system. ..
  14. Ohba A, Mizushima T, Katayama T, Sekimizu K. Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli. FEBS Lett. 1997;404:125-8 pubmed
    ..As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins. ..
  15. Lee H, Chi S, Kang M, Baek K, Kim H. Stability and folding of precursor and mature tryptophan-substituted ribose binding protein of Escherichia coli. Arch Biochem Biophys. 1996;328:78-84 pubmed
    ..Refolding kinetics of the WT-RBP and Trp-RBP showed a two-step reaction when monitored by fluorescence and by CD. ..
  16. Lopilato J, Garwin J, Emr S, Silhavy T, Beckwith J. D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. J Bacteriol. 1984;158:665-73 pubmed
    We have isolated mutants defective in high-affinity D-ribose transport. The mutations map in rbsT or rbsB , the structural gene for ribose binding protein...
  17. Prajapati R, Indu S, Varadarajan R. Identification and thermodynamic characterization of molten globule states of periplasmic binding proteins. Biochemistry. 2007;46:10339-52 pubmed
    ..The ability of these sequentially unrelated proteins to form highly ordered molten globules may be related to their large size as well as an intrinsic property of periplasmic binding protein folds. ..
  18. Han M, Lee J, Lee S, Yoo J. Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production. J Biomed Biotechnol. 2008;2008:735101 pubmed publisher
    ..showing altered expression levels with oleic acid presence, 9 proteins including AldA, Cdd, FadA, FadB, FadL, MalE, RbsB, Udp, and YccU were newly synthesized...
  19. Clifton M, Simon M, Erramilli S, Zhang H, Zaitseva J, Hermodson M, et al. In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes. J Biol Chem. 2015;290:5555-65 pubmed publisher
    ..nucleotide binding domains; a transmembrane domain homodimer, RbsC2; and a periplasmic substrate binding protein, RbsB. To investigate the transport mechanism of the complex RbsABC2, we probed intersubunit interactions by varying the ..