Gene Symbol: queF
Description: 7-cyano-7-deazaguanine reductase (NADPH-dependent)
Alias: ECK2789, JW2765, yqcD
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Yokoyama S, Miyazawa T, Iitaka Y, Yamaizumi Z, Kasai H, Nishimura S. Three-dimensional structure of hyper-modified nucleoside Q located in the wobbling position of tRNA. Nature. 1979;282:107-9 pubmed
    ..1). The unique structural features of Q are the unusual cyclopentenediol side chain and the 7-deazaguanine ring. Queuosine is one of the most complicated modified nucleosides found thus far. ..
  2. Kasai H, Oashi Z, Harada F, Nishimura S, Oppenheimer N, Crain P, et al. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine. Biochemistry. 1975;14:4198-208 pubmed
    ..The structure of Q is unique since it is a derivative of 7-deazaguanosine having cyclopentenediol in the side chain at the C-7 position. This is the first example of purine skeleton modification in a nucleoside from tRNA. ..
  3. Harada F, Nishimura S. Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids. Biochemistry. 1972;11:301-8 pubmed
  4. Noguchi S, Nishimura Y, Hirota Y, Nishimura S. Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tRNA. J Biol Chem. 1982;257:6544-50 pubmed
    ..coli for survival in the natural environment. ..
  5. Iwata Reuyl D. Biosynthesis of the 7-deazaguanosine hypermodified nucleosides of transfer RNA. Bioorg Chem. 2003;31:24-43 pubmed
  6. Reader J, Metzgar D, Schimmel P, De Crecy Lagard V. Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine. J Biol Chem. 2004;279:6280-5 pubmed
    ..Thus, the products of these four genes (named queC, -D, -E, and -F) are essential for the Q biosynthetic pathway. ..
  7. Van Lanen S, Reader J, Swairjo M, De Crecy Lagard V, Lee B, Iwata Reuyl D. From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold. Proc Natl Acad Sci U S A. 2005;102:4264-9 pubmed
    ..b>QueF exhibits significant homology to the type I GTP cyclohydrolases characterized by FolE...
  8. Gaur R, Varshney U. Genetic analysis identifies a function for the queC (ybaX) gene product at an initial step in the queuosine biosynthetic pathway in Escherichia coli. J Bacteriol. 2005;187:6893-901 pubmed
    ..In addition, we discuss the possibilities of collaboration of QueC with other cellular proteins in the production of preQ0. ..
  9. De Crecy Lagard V. Identification of genes encoding tRNA modification enzymes by comparative genomics. Methods Enzymol. 2007;425:153-83 pubmed
    ..Methods that link gene and function but do not rely on sequence homology will be detailed, with examples taken from the tRNA modification field. ..

More Information


  1. Jung J, Czabany T, Wilding B, Klempier N, Nidetzky B. Kinetic Analysis and Probing with Substrate Analogues of the Reaction Pathway of the Nitrile Reductase QueF from Escherichia coli. J Biol Chem. 2016;291:25411-25426 pubmed
    The enzyme QueF catalyzes a four-electron reduction of a nitrile group into an amine, the only reaction of this kind known in biology...
  2. Moeller K, Nguyen G, Hollmann F, Hanefeld U. Expression and characterization of the nitrile reductase queF from E. coli. Enzyme Microb Technol. 2013;52:129-33 pubmed publisher
    ..The enzyme activity towards its natural substrate, preQ(0), was demonstrated and optimal working conditions were found to be at 37°C and at pH 7 with Tris buffer. ..