Gene Symbol: queA
Description: S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Alias: ECK0399, JW0395
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. De Crecy Lagard V. Identification of genes encoding tRNA modification enzymes by comparative genomics. Methods Enzymol. 2007;425:153-83 pubmed
    ..Methods that link gene and function but do not rely on sequence homology will be detailed, with examples taken from the tRNA modification field. ..
  2. Gaur R, Varshney U. Genetic analysis identifies a function for the queC (ybaX) gene product at an initial step in the queuosine biosynthetic pathway in Escherichia coli. J Bacteriol. 2005;187:6893-901 pubmed
    ..coli B105. Further, analyses of tRNA(Tyr) from E. coli JE10651 (queA mutant), its derivative generated by deletion of chromosomal queC (queA deltaqueC), and E...
  3. Van Lanen S, Kinzie S, Matthieu S, Link T, Culp J, Iwata Reuyl D. tRNA modification by S-adenosylmethionine:tRNA ribosyltransferase-isomerase. Assay development and characterization of the recombinant enzyme. J Biol Chem. 2003;278:10491-9 pubmed publisher
    ..coli queA deletion mutant to allow for the isolation of large quantities of substrate tRNA, and [U-ribosyl-(14)C]AdoMet was ..
  4. Yokoyama S, Miyazawa T, Iitaka Y, Yamaizumi Z, Kasai H, Nishimura S. Three-dimensional structure of hyper-modified nucleoside Q located in the wobbling position of tRNA. Nature. 1979;282:107-9 pubmed
    ..1). The unique structural features of Q are the unusual cyclopentenediol side chain and the 7-deazaguanine ring. Queuosine is one of the most complicated modified nucleosides found thus far. ..
  5. Van Lanen S, Iwata Reuyl D. Kinetic mechanism of the tRNA-modifying enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA). Biochemistry. 2003;42:5312-20 pubmed
    The bacterial enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) catalyzes the unprecedented transfer and isomerization of the ribosyl moiety of S-adenosylmethionine (AdoMet) to a modified tRNA nucleoside in the ..
  6. Slany R, Bosl M, Crain P, Kersten H. A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine. Biochemistry. 1993;32:7811-7 pubmed
    ..ring, is formed from tRNA precursors containing 7-(aminomethyl)-7-deazaguanine (preQ1) by the queA gene product. A genomic queA mutant accumulating preQ1 tRNA was constructed...
  7. Kasai H, Oashi Z, Harada F, Nishimura S, Oppenheimer N, Crain P, et al. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine. Biochemistry. 1975;14:4198-208 pubmed
    ..The structure of Q is unique since it is a derivative of 7-deazaguanosine having cyclopentenediol in the side chain at the C-7 position. This is the first example of purine skeleton modification in a nucleoside from tRNA. ..
  8. Slany R, Kersten H. The promoter of the tgt/sec operon in Escherichia coli is preceded by an upstream activation sequence that contains a high affinity FIS binding site. Nucleic Acids Res. 1992;20:4193-8 pubmed
    The tgt/sec operon in E. coli consists of five genes: queA, tgt, ORF12, secD, and secF. QueA and Tgt participate in the biosynthesis of the hypermodified t-RNA nucleoside Queuosine, whereas SecD and SecF are involved in protein secretion...
  9. Noguchi S, Nishimura Y, Hirota Y, Nishimura S. Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tRNA. J Biol Chem. 1982;257:6544-50 pubmed
    ..coli for survival in the natural environment. ..

More Information


  1. Pogliano K, Beckwith J. Genetic and molecular characterization of the Escherichia coli secD operon and its products. J Bacteriol. 1994;176:804-14 pubmed
    ..this operon, and found that, in addition to secD and secF, it contains the upstream gene yajC, but not the genes queA or tgt, in contrast to previous reports...
  2. Dineshkumar T, Thanedar S, Subbulakshmi C, Varshney U. An unexpected absence of queuosine modification in the tRNAs of an Escherichia coli B strain. Microbiology. 2002;148:3779-87 pubmed
    ..However, the lack of queuosine in tRNAs was not a common feature of all E. coli B strains. The tgt and queA genes in B105 were shown to be functional by their ability to complement tgt and queA mutant strains...
  3. Reuter K, Slany R, Ullrich F, Kersten H. Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes. J Bacteriol. 1991;173:2256-64 pubmed
    ..This gene was designated queA. The genes are arranged in the following order: ORF 14 (transcribed in the counterclockwise direction), queA, tgt, ..
  4. Okada N, Noguchi S, Nishimura S, Ohgi T, Goto T, Crain P, et al. Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine. Nucleic Acids Res. 1978;5:2289-96 pubmed
    ..The same molecule is also found in tRNA of an E. coli mutant selected for deficient synthesis of modified nucleosides. ..
  5. Phillipson D, Edmonds C, Crain P, Smith D, Davis D, McCloskey J. Isolation and structure elucidation of an epoxide derivative of the hypermodified nucleoside queuosine from Escherichia coli transfer RNA. J Biol Chem. 1987;262:3462-71 pubmed
    ..The present finding constitutes the first report of epoxide formation during post-transcriptional processing of RNA. ..
  6. Grimm C, Klebe G, Ficner R, Reuter K. Screening orthologs as an important variable in crystallization: preliminary X-ray diffraction studies of the tRNA-modifying enzyme S-adenosyl-methionine:tRNA ribosyl transferase/isomerase. Acta Crystallogr D Biol Crystallogr. 2000;56:484-8 pubmed
    The genes encoding the tRNA-modifying enzyme S-adenosylmethionine:tRNA ribosyl transferase/isomerase (QueA) from 12 eubacterial sources were overexpressed in Escherichia coli and the resulting products were purified to homogeneity and ..
  7. Reczkowski R, Markham G. Structural and functional roles of cysteine 90 and cysteine 240 in S-adenosylmethionine synthetase. J Biol Chem. 1995;270:18484-90 pubmed
    ..Remarkably all of the mutants are much more thermally stable than the wild type enzyme. ..
  8. Harada F, Nishimura S. Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids. Biochemistry. 1972;11:301-8 pubmed
  9. Slany R, Bosl M, Kersten H. Transfer and isomerization of the ribose moiety of AdoMet during the biosynthesis of queuosine tRNAs, a new unique reaction catalyzed by the QueA protein from Escherichia coli. Biochimie. 1994;76:389-93 pubmed
    The enzyme QueA of E coli is involved in the biosynthesis of the hypermodified tRNA nucleoside queuosine...
  10. Kinzie S, Thern B, Iwata Reuyl D. Mechanistic studies of the tRNA-modifying enzyme QueA: a chemical imperative for the use of AdoMet as a "ribosyl" donor. Org Lett. 2000;2:1307-10 pubmed
    formula: see text] The enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) catalyzes the penultimate step in the biosynthesis of the tRNA nucleoside queuosine, a unique ribosyl transfer from the cofactor S-..
  11. Lucht J, Boos W, Bremer E. Alignment of genes from the 9-minute region (araJ to tsx) of the Escherichia coli K-12 linkage map to the physical map. J Bacteriol. 1992;174:1709-10 pubmed
  12. Iwata Reuyl D. Biosynthesis of the 7-deazaguanosine hypermodified nucleosides of transfer RNA. Bioorg Chem. 2003;31:24-43 pubmed
    ..guanine in the biosynthesis of both nucleosides, and S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA), which catalyzes the penultimate step in the biosynthesis of queuosine, the construction of the carbocyclic side ..
  13. Frey B, McCloskey J, Kersten W, Kersten H. New function of vitamin B12: cobamide-dependent reduction of epoxyqueuosine to queuosine in tRNAs of Escherichia coli and Salmonella typhimurium. J Bacteriol. 1988;170:2078-82 pubmed
    ..Under these conditions, the biosynthesis of cobalamin was induced. The results suggest that oQ is derived from ribose and that oQ is finally reduced to Q by a cobamide-dependent enzyme. ..
  14. Mueller S, Slany R. Structural analysis of the interaction of the tRNA modifying enzymes Tgt and QueA with a substrate tRNA. FEBS Lett. 1995;361:259-64 pubmed
    The enzymes tRNA guanine-transglycosylase (Tgt) and S-adenosylmethionine :tRNA ribosyltransferase-isomerase (QueA) participate in the biosynthesis of the hypermodified tRNA nucleoside queuosine (Q) in Escherichia coli...