putP

Summary

Gene Symbol: putP
Description: proline:sodium symporter
Alias: ECK1006, JW1001, putC
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Nelson K, Selander R. Evolutionary genetics of the proline permease gene (putP) and the control region of the proline utilization operon in populations of Salmonella and Escherichia coli. J Bacteriol. 1992;174:6886-95 pubmed
    Virtually complete sequences (1,467 bp) of the proline permease gene (putP) and complete sequences (416 to 422 bp) of the control region of the proline utilization operon were determined for 16 strains of Salmonella, representing all ..
  2. Wood J, Zadworny D. Amplification of the put genes and identification of the put gene products in Escherichia coli K12. Can J Biochem. 1980;58:787-96 pubmed
    ..PP-I is inactivated by mutations at putP and the bifunctional dehydrogenase is encoded in the adjacent locus, putA, at 22 min on the chromosome map...
  3. Yamato I, Anraku Y. Site-specific alteration of cysteine 281, cysteine 344, and cysteine 349 in the proline carrier of Escherichia coli. J Biol Chem. 1988;263:16055-7 pubmed
    ..These results indicate that these cysteine residues are not essential for proline transport and that Cys-281 and Cys-344 may be close to the substrate-binding site that contains an N-ethylmaleimide-sensitive residue. ..
  4. Bracher S, Schmidt C, Dittmer S, Jung H. Core Transmembrane Domain 6 Plays a Pivotal Role in the Transport Cycle of the Sodium/Proline Symporter PutP. J Biol Chem. 2016;291:26208-26215 pubmed
    ..Here, the function of TM6' in the sodium/proline symporter PutP, a member of the solute/sodium symporter family, was investigated...
  5. Zhou A, Wozniak A, Meyer Lipp K, Nietschke M, Jung H, Fendler K. Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli. J Mol Biol. 2004;343:931-42 pubmed
    Charge translocation associated with the activity of the Na(+)/proline cotransporter PutP of Escherichia coli was analyzed for the first time...
  6. Ohsawa M, Mogi T, Yamamoto H, Yamato I, Anraku Y. Proline carrier mutant of Escherichia coli K-12 with altered cation sensitivity of substrate-binding activity: cloning, biochemical characterization, and identification of the mutation. J Bacteriol. 1988;170:5185-91 pubmed
    Two putP mutants of Escherichia coli K-12 that were defective in proline transport but retained the binding activities of the major proline carrier were isolated (T. Mogi, H. Yamamoto, T. Nakao, I. Yamato, and Y. Anraku, Mol. Gen. Genet...
  7. Chen C, Wilson T. Solubilization and functional reconstitution of the proline transport system of Escherichia coli. J Biol Chem. 1986;261:2599-604 pubmed
    The membrane carrier for L-proline (product of the putP gene) of Escherichia coli K12 was solubilized and functionally reconstituted with E. coli phospholipid by the cholate dilution method...
  8. Wegener C, Tebbe S, Steinhoff H, Jung H. Spin labeling analysis of structure and dynamics of the Na(+)/proline transporter of Escherichia coli. Biochemistry. 2000;39:4831-7 pubmed
    ..the functional importance attributed to the N-terminal part of the Na(+)/proline transporter of Escherichia coli (PutP), we report here on the structural arrangement and functional dynamics of transmembrane domains (TMs) II and III ..
  9. Reed J, Patel T, Chen K, Joyce A, Applebee M, Herring C, et al. Systems approach to refining genome annotation. Proc Natl Acad Sci U S A. 2006;103:17480-4 pubmed
    ..experimentally in five cases, leading to the functional assignment of eight ORFs (yjjLMN, yeaTU, dctA, idnT, and putP) with two new enzymatic activities and four transport functions...

More Information

Publications35

  1. Jung H. The sodium/substrate symporter family: structural and functional features. FEBS Lett. 2002;529:73-7 pubmed
  2. Jung H. Towards the molecular mechanism of Na(+)/solute symport in prokaryotes. Biochim Biophys Acta. 2001;1505:131-43 pubmed
    ..Furthermore, an amino acid substitution analysis of the Na(+)/proline transporter (PutP) of Escherichia coli, a member of the SSF, has identified regions of particular functional importance...
  3. Reizer J, Reizer A, Saier M. A functional superfamily of sodium/solute symporters. Biochim Biophys Acta. 1994;1197:133-66 pubmed
    ..This study serves to clarify structural, functional and evolutionary relationships among eleven distinct families of functionally related transport proteins. ..
  4. Hilger D, Polyhach Y, Jung H, Jeschke G. Backbone structure of transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli. Biophys J. 2009;96:217-25 pubmed publisher
    ..3 kDa Na(+)/proline symporter PutP of Escherichia coli. The conformational distribution of the spin labels is accounted for by a rotamer library...
  5. Yamato I, Kotani M, Oka Y, Anraku Y. Site-specific alteration of arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli. J Biol Chem. 1994;269:5720-4 pubmed
    ..The amounts of the carrier proteins expressed from these mutated putP genes were similar to that from the wild-type gene...
  6. Stalmach M, Grothe S, Wood J. Two proline porters in Escherichia coli K-12. J Bacteriol. 1983;156:481-6 pubmed
    Escherichia coli mutants defective at putP and putA lack proline transport via proline porter I and proline dehydrogenase activity, respectively...
  7. Tristram H, Neale S. The activity and specificity of the proline permease in wild-type and analogue-resistant strains of Escherichia coli. J Gen Microbiol. 1968;50:121-37 pubmed
  8. Wood J, Zadworny D. Characterization of an inducible porter required for L-proline catabolism by Escherichia coli K12. Can J Biochem. 1979;57:1191-9 pubmed
    ..A strain defective for L-proline uptake due to a lesion at the locus putP does not show elevated uptake activity either on relief of catabolite repression or when grown on glycyl-L-proline ..
  9. Olkhova E, Raba M, Bracher S, Hilger D, Jung H. Homology model of the Na+/proline transporter PutP of Escherichia coli and its functional implications. J Mol Biol. 2011;406:59-74 pubmed publisher
    ..and evolutionary conservation analysis to construct and validate a first model of the Na(+)/proline symporter PutP of Escherichia coli based on the crystal structure of the bacterial Na(+)/galactose symporter vSGLT...
  10. Raba M, Baumgartner T, Hilger D, Klempahn K, Härtel T, Jung K, et al. Function of transmembrane domain IX in the Na+/proline transporter PutP. J Mol Biol. 2008;382:884-93 pubmed publisher
    ..Using the Na(+)/proline transporter PutP as a model, a complete Cys scanning mutagenesis of TM IX (positions 324 to 351) was performed here to further ..
  11. Saidijam M, Bettaney K, Szakonyi G, Psakis G, Shibayama K, Suzuki S, et al. Active membrane transport and receptor proteins from bacteria. Biochem Soc Trans. 2005;33:867-72 pubmed
  12. Pirch T, Quick M, Nietschke M, Langkamp M, Jung H. Sites important for Na+ and substrate binding in the Na+/proline transporter of Escherichia coli, a member of the Na+/solute symporter family. J Biol Chem. 2002;277:8790-6 pubmed
    To elucidate the functional importance of transmembrane domain II in the Na(+)/proline transporter (PutP) of Escherichia coli we analyzed the effect of replacing Ser-54 through Gly-58...
  13. Jung H, Rübenhagen R, Tebbe S, Leifker K, Tholema N, Quick M, et al. Topology of the Na+/proline transporter of Escherichia coli. J Biol Chem. 1998;273:26400-7 pubmed
    Hydropathy profile analysis of the amino acid sequence of the Na+/proline transporter of Escherichia coli (PutP) suggests that the protein consists of 12 transmembrane domains (TMs) which are connected by hydrophilic loops (Nakao, T...
  14. Jung H. Topology and function of the Na+/proline transporter of Escherichia coli, a member of the Na+/solute cotransporter family. Biochim Biophys Acta. 1998;1365:60-4 pubmed
    The Na+/proline transporter of Escherichia coli (PutP) is a member of the Na+/solute contransporter family (SCF) and catalyzes the uptake of proline by a Na+ dependent transport mechanism...
  15. Quick M, Tebbe S, Jung H. Ser57 in the Na+/proline permease of Escherichia coli is critical for high-affinity proline uptake. Eur J Biochem. 1996;239:732-6 pubmed
    ..coli has been replaced with alanine, cysteine, glycine, or threonine, and properties of the corresponding putP mutants have been analyzed...
  16. Nakao T, Yamato I, Anraku Y. Nucleotide sequence of putC, the regulatory region for the put regulon of Escherichia coli K12. Mol Gen Genet. 1987;210:364-8 pubmed
    The nucleotide sequence of the putC region, from which divergent transcription of the putP and putA genes starts, was determined...
  17. Jeschke G, Wegener C, Nietschke M, Jung H, Steinhoff H. Interresidual distance determination by four-pulse double electron-electron resonance in an integral membrane protein: the Na+/proline transporter PutP of Escherichia coli. Biophys J. 2004;86:2551-7 pubmed
    Proximity relationships within three doubly spin-labeled variants of the Na+/proline transporter PutP of Escherichia coli were studied by means of four-pulse double electron-electron resonance spectroscopy. The large value of 4...
  18. Hilger D, Böhm M, Hackmann A, Jung H. Role of Ser-340 and Thr-341 in transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli in ligand binding and transport. J Biol Chem. 2008;283:4921-9 pubmed
    ..Using the Na+/proline transporter PutP of Escherichia coli as a model, the role of two conserved residues, Ser-340 and Thr-341, is investigated to obtain ..
  19. Hanada K, Yoshida T, Yamato I, Anraku Y. Sodium ion and proline binding sites in the Na+/proline symport carrier of Escherichia coli. Biochim Biophys Acta. 1992;1105:61-6 pubmed
    ..These results indicate that Cys-344, which is located in the putative eighth membrane-spanning domain in the carrier, is a cysteine residue functionally involved in the high-affinity binding for sodium ion and proline. ..
  20. Ganea C, Fendler K. Bacterial transporters: charge translocation and mechanism. Biochim Biophys Acta. 2009;1787:706-13 pubmed publisher
    ..In melibiose permease MelB and the Na(+)/proline carrier PutP pre-steady-state charge displacements can be assigned to an electrogenic conformational transition associated with ..
  21. Nakao T, Yamato I, Anraku Y. Nucleotide sequence of putP, the proline carrier gene of Escherichia coli K12. Mol Gen Genet. 1987;208:70-5 pubmed
    The nucleotide sequence of the putP gene coding for the proline carrier in Escherichia coli has been determined and the amino acid sequence of the proline carrier deduced from it...
  22. Borlaug B, Melenovsky V, Russell S, Kessler K, Pacak K, Becker L, et al. Impaired chronotropic and vasodilator reserves limit exercise capacity in patients with heart failure and a preserved ejection fraction. Circulation. 2006;114:2138-47 pubmed
    ..These limitations cannot be ascribed to diastolic abnormalities per se and may provide novel therapeutic targets for interventions to improve exercise capacity in this disorder. ..
  23. Jung H, Tebbe S, Schmid R, Jung K. Unidirectional reconstitution and characterization of purified Na+/proline transporter of Escherichia coli. Biochemistry. 1998;37:11083-8 pubmed
    ..for large-scale purification and unidirectional reconstitution of the Na+/proline transporter of Escherichia coli (PutP) is described...
  24. Wood J. Genetics of L-proline utilization in Escherichia coli. J Bacteriol. 1981;146:895-901 pubmed
    ..Mutants resistant to AC and DHP are defective for proline transport alone (putP mutants), and mutants resistant to AC but not to DHP are defective both in putP and in the closely linked proline ..
  25. Mogi T, Yamamoto H, Nakao T, Yamato I, Anraku Y. Genetic and physical characterization of putP, the proline carrier gene of Escherichia coli K12. Mol Gen Genet. 1986;202:35-41 pubmed
    ..coli genetic map (Bachmann 1983) as a composite gene in the proline utilization gene cluster, putP, putC, and putA, arranged in this order...
  26. Yamato I, Ohsawa M, Anraku Y. Defective cation-coupling mutants of Escherichia coli Na+/proline symport carrier. Characterization and localization of mutations. J Biol Chem. 1990;265:2450-5 pubmed
    A major proline carrier in Escherichia coli encoded by the putP gene mediates proline/Na+ or Li+ symport...