Gene Symbol: pstC
Description: phosphate ABC transporter permease
Alias: ECK3720, JW3705, phoW
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Webb D, Rosenberg H, Cox G. Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices. J Biol Chem. 1992;267:24661-8 pubmed
    ..of the requirement for function of specific charged residues or proline residues in the two hydrophobic subunits (PstC and PstA) has been carried out...
  2. Cox G, Webb D, Rosenberg H. Specific amino acid residues in both the PstB and PstC proteins are required for phosphate transport by the Escherichia coli Pst system. J Bacteriol. 1989;171:1531-4 pubmed
    Three mutant alleles of the pstC gene and one mutant allele of the pstB gene were produced by site-directed mutagenesis...
  3. Amemura M, Makino K, Shinagawa H, Kobayashi A, Nakata A. Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli. J Mol Biol. 1985;184:241-50 pubmed
    ..Therefore, we propose to designate the allele phoW. The order of the genes in this region has been established to be phoS-phoW-pstA(=phoT)-pstB-phoU counterclockwise ..
  4. Surin B, Rosenberg H, Cox G. Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships. J Bacteriol. 1985;161:189-98 pubmed
    ..The complete sequence is transcribed counterclockwise, in the order phoS pstC pstA pstB phoU...
  5. Amemura M, Shinagawa H, Makino K, Otsuji N, Nakata A. Cloning of and complementation tests with alkaline phosphatase regulatory genes (phoS and phoT) of Escherichia coli. J Bacteriol. 1982;152:692-701 pubmed
  6. Xavier K, Kossmann M, Santos H, Boos W. Kinetic analysis by in vivo 31P nuclear magnetic resonance of internal Pi during the uptake of sn-glycerol-3-phosphate by the pho regulon-dependent Ugp system and the glp regulon-dependent GlpT system. J Bacteriol. 1995;177:699-704 pubmed
    ..Both systems were inversely controlled by internal Pi. Whereas the Ugp system was inhibited, the GlpT system was stimulated by elevated internal Pi. ..
  7. Steed P, Wanner B. Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon. J Bacteriol. 1993;175:6797-809 pubmed
    ..Further, our results contradict those of M. Muda, N. N. Rao, and A. Torriani (J. Bacteriol. 174:8057-8064, 1992), who reported that the PhoU protein was required for Pi uptake. ..
  8. Aguena M, Yagil E, Spira B. Transcriptional analysis of the pst operon of Escherichia coli. Mol Genet Genomics. 2002;268:518-24 pubmed
    ..operon of Escherichia coli, which encodes the phosphate-specific transport system, is composed of five genes, pstS, pstC, pstA, pstB and phoU, whose transcription is induced by phosphate starvation...
  9. Rosen B, Liu Z. Transport pathways for arsenic and selenium: a minireview. Environ Int. 2009;35:512-5 pubmed publisher
    ..In this review we discuss the membrane proteins that transport arsenic and selenium into cells, from bacteria to humans, as well as some of the efflux proteins involved in detoxification. ..

More Information


  1. Rosenberg H, Gerdes R, Chegwidden K. Two systems for the uptake of phosphate in Escherichia coli. J Bacteriol. 1977;131:505-11 pubmed
    ..In strains in which both systems were fully operative, the initial rates of uptake was nearly additive, and the systems appeared to interact with a common intracellular phosphate pool. ..
  2. Rao N, Torriani A. Molecular aspects of phosphate transport in Escherichia coli. Mol Microbiol. 1990;4:1083-90 pubmed
    ..The channel consists of two proteins, PstA and PstC, which have six and five transmembrane helices, respectively...
  3. Torriani A. From cell membrane to nucleotides: the phosphate regulon in Escherichia coli. Bioessays. 1990;12:371-6 pubmed
    ..alkaline phosphatase) by high Pi concentrations in the medium requires the presence of an intact Pst operon (pstS, pstC, pstA, pstB and phoU) and phoR...
  4. Cox G, Webb D, Godovac Zimmermann J, Rosenberg H. Arg-220 of the PstA protein is required for phosphate transport through the phosphate-specific transport system in Escherichia coli but not for alkaline phosphatase repression. J Bacteriol. 1988;170:2283-6 pubmed
    ..Neither the Arg-170----Gln nor the Glu-173----Gln mutation affected phosphate transport. The results are discussed in relation to a proposed structure of the PstA protein. ..
  5. Magota K, Otsuji N, Miki T, Horiuchi T, Tsunasawa S, Kondo J, et al. Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli. J Bacteriol. 1984;157:909-17 pubmed
    ..the nucleotide sequence of the regulatory region of phoS was compared with those of phoA and phoE, the genes constituting the pho regulon. ..
  6. Cox G, Rosenberg H, Downie J, Silver S. Genetic analysis of mutants affected in the Pst inorganic phosphate transport system. J Bacteriol. 1981;148:1-9 pubmed
    ..The gene order was concluded to be pstA-(pstB-phoT)-phoS, with the pstA gene promotor proximal and the direction of transcription opposite to that of the nearby unc operon. ..
  7. Aguena M, Spira B. RT-PCR of long prokaryotic operon transcripts without DNase treatment. J Microbiol Methods. 2003;55:419-23 pubmed
    ..To avoid amplification of genomic DNA, the hybrid-primer molecules that were not incorporated into the first-strand cDNA were removed by RNase H treatment followed by ultrafiltration...