Gene Symbol: pstB
Description: phosphate ABC transporter ATPase
Alias: ECK3718, JW3703, phoT
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Gardner S, Johns K, Tanner R, McCleary W. The PhoU protein from Escherichia coli interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane. J Bacteriol. 2014;196:1741-52 pubmed publisher
    ..experiments that PhoU interacts with PhoR through its PAS (Per-ARNT-Sim) domain and that it also interacts with PstB, the cytoplasmic component of the transporter...
  2. Cox G, Webb D, Rosenberg H. Specific amino acid residues in both the PstB and PstC proteins are required for phosphate transport by the Escherichia coli Pst system. J Bacteriol. 1989;171:1531-4 pubmed
    Three mutant alleles of the pstC gene and one mutant allele of the pstB gene were produced by site-directed mutagenesis...
  3. Willsky G, Bennett R, Malamy M. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J Bacteriol. 1973;113:529-39 pubmed
    Two classes of alkaline phosphatase constitutive mutations which comprise the original phoS locus (genes phoS and phoT) on the Escherichia coli genome have been implicated in the regulation of alkaline phosphatase synthesis...
  4. Amemura M, Shinagawa H, Makino K, Otsuji N, Nakata A. Cloning of and complementation tests with alkaline phosphatase regulatory genes (phoS and phoT) of Escherichia coli. J Bacteriol. 1982;152:692-701 pubmed
    The regulatory genes of alkaline phosphatase, phoS and phoT, of Escherichia coli were cloned on pBR322, initially as an 11.8-kilobase EcoRI fragment. A restriction map of the hybrid plasmid was established...
  5. Magota K, Otsuji N, Miki T, Horiuchi T, Tsunasawa S, Kondo J, et al. Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli. J Bacteriol. 1984;157:909-17 pubmed
    ..the nucleotide sequence of the regulatory region of phoS was compared with those of phoA and phoE, the genes constituting the pho regulon. ..
  6. Rosen B, Liu Z. Transport pathways for arsenic and selenium: a minireview. Environ Int. 2009;35:512-5 pubmed publisher
    ..In this review we discuss the membrane proteins that transport arsenic and selenium into cells, from bacteria to humans, as well as some of the efflux proteins involved in detoxification. ..
  7. Aguena M, Yagil E, Spira B. Transcriptional analysis of the pst operon of Escherichia coli. Mol Genet Genomics. 2002;268:518-24 pubmed
    ..coli, which encodes the phosphate-specific transport system, is composed of five genes, pstS, pstC, pstA, pstB and phoU, whose transcription is induced by phosphate starvation...
  8. Cervera J, Bendala E, Britton H, Bueso J, Nassif Z, Lusty C, et al. Photoaffinity labeling with UMP of lysine 992 of carbamyl phosphate synthetase from Escherichia coli allows identification of the binding site for the pyrimidine inhibitor. Biochemistry. 1996;35:7247-55 pubmed
    ..We conclude that sequence changes in the binding fold provide a structural basis for the different regulatory properties found among CPSs I, II, and III. ..
  9. Steed P, Wanner B. Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon. J Bacteriol. 1993;175:6797-809 pubmed
    ..These mutants were used to show that delta (pstSCAB-phoU) and delta (pstB-phoU) mutations abolished Pi uptake by the PstSCAB transporter, as expected, and that delta phoU mutations had no ..

More Information


  1. Xavier K, Kossmann M, Santos H, Boos W. Kinetic analysis by in vivo 31P nuclear magnetic resonance of internal Pi during the uptake of sn-glycerol-3-phosphate by the pho regulon-dependent Ugp system and the glp regulon-dependent GlpT system. J Bacteriol. 1995;177:699-704 pubmed
    ..Both systems were inversely controlled by internal Pi. Whereas the Ugp system was inhibited, the GlpT system was stimulated by elevated internal Pi. ..
  2. Surin B, Rosenberg H, Cox G. Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships. J Bacteriol. 1985;161:189-98 pubmed
    ..The complete sequence is transcribed counterclockwise, in the order phoS pstC pstA pstB phoU...
  3. Amemura M, Makino K, Shinagawa H, Kobayashi A, Nakata A. Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli. J Mol Biol. 1985;184:241-50 pubmed
    The pstA(=phoT), pstB and phoU genes are situated at 84 minutes on the Escherichia coli genetic map...
  4. Cox G, Rosenberg H, Downie J, Silver S. Genetic analysis of mutants affected in the Pst inorganic phosphate transport system. J Bacteriol. 1981;148:1-9 pubmed
    ..The two alleles phoS28 and phoS21 appeared to be polar. The phoS28 allele affected both the phoT and pstB genes but not the pstA gene, whereas the phoS21 allele appeared to be a mutation in the pstA gene exerting ..
  5. Torriani A. From cell membrane to nucleotides: the phosphate regulon in Escherichia coli. Bioessays. 1990;12:371-6 pubmed high Pi concentrations in the medium requires the presence of an intact Pst operon (pstS, pstC, pstA, pstB and phoU) and phoR...
  6. Rao N, Torriani A. Molecular aspects of phosphate transport in Escherichia coli. Mol Microbiol. 1990;4:1083-90 pubmed
    ..On the cytoplasmic side of the membrane the channel is linked to the PstB protein, which carries a nucleotide (probably ATP)-binding site...
  7. Rosenberg H, Gerdes R, Chegwidden K. Two systems for the uptake of phosphate in Escherichia coli. J Bacteriol. 1977;131:505-11 pubmed
    ..In strains in which both systems were fully operative, the initial rates of uptake was nearly additive, and the systems appeared to interact with a common intracellular phosphate pool. ..
  8. Levitz R, Friedberg I, Brucker R, Fux A, Yagil E. The effect of the locus pstB on phosphate binding in the phosphate specific transport (PST) system of Escherichia coli. Mol Gen Genet. 1985;200:118-22 pubmed
    ..The binding of Pi to periplasmic protein(s) and the kinetic parameters of Pi uptake were studied in phoT and pstB mutants of E. coli...
  9. Chan F, Torriani A. PstB protein of the phosphate-specific transport system of Escherichia coli is an ATPase. J Bacteriol. 1996;178:3974-7 pubmed
    The PstB protein of the phosphate-specific transport (Pst) system of Escherichia coli bound and hydrolyzed ATP, producing ADP. Urea-treated denatured PstB did not bind ATP...
  10. Aguena M, Spira B. RT-PCR of long prokaryotic operon transcripts without DNase treatment. J Microbiol Methods. 2003;55:419-23 pubmed
    ..To avoid amplification of genomic DNA, the hybrid-primer molecules that were not incorporated into the first-strand cDNA were removed by RNase H treatment followed by ultrafiltration...