Gene Symbol: pstA
Description: phosphate ABC transporter permease
Alias: ECK3719, JW3704, R2pho, phoR2b, phoT
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Webb D, Rosenberg H, Cox G. Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices. J Biol Chem. 1992;267:24661-8 pubmed
    ..requirement for function of specific charged residues or proline residues in the two hydrophobic subunits (PstC and PstA) has been carried out...
  2. Xavier K, Kossmann M, Santos H, Boos W. Kinetic analysis by in vivo 31P nuclear magnetic resonance of internal Pi during the uptake of sn-glycerol-3-phosphate by the pho regulon-dependent Ugp system and the glp regulon-dependent GlpT system. J Bacteriol. 1995;177:699-704 pubmed
    ..Both systems were inversely controlled by internal Pi. Whereas the Ugp system was inhibited, the GlpT system was stimulated by elevated internal Pi. ..
  3. Cox G, Rosenberg H, Downie J, Silver S. Genetic analysis of mutants affected in the Pst inorganic phosphate transport system. J Bacteriol. 1981;148:1-9 pubmed
    ..The two alleles phoS28 and phoS21 appeared to be polar. The phoS28 allele affected both the phoT and pstB genes but not the pstA gene, whereas the phoS21 allele appeared to be a mutation in the pstA gene exerting ..
  4. Amemura M, Shinagawa H, Makino K, Otsuji N, Nakata A. Cloning of and complementation tests with alkaline phosphatase regulatory genes (phoS and phoT) of Escherichia coli. J Bacteriol. 1982;152:692-701 pubmed
    The regulatory genes of alkaline phosphatase, phoS and phoT, of Escherichia coli were cloned on pBR322, initially as an 11.8-kilobase EcoRI fragment. A restriction map of the hybrid plasmid was established...
  5. Willsky G, Bennett R, Malamy M. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J Bacteriol. 1973;113:529-39 pubmed
    Two classes of alkaline phosphatase constitutive mutations which comprise the original phoS locus (genes phoS and phoT) on the Escherichia coli genome have been implicated in the regulation of alkaline phosphatase synthesis...
  6. Rao N, Torriani A. Molecular aspects of phosphate transport in Escherichia coli. Mol Microbiol. 1990;4:1083-90 pubmed
    ..The channel consists of two proteins, PstA and PstC, which have six and five transmembrane helices, respectively...
  7. Cox G, Webb D, Godovac Zimmermann J, Rosenberg H. Arg-220 of the PstA protein is required for phosphate transport through the phosphate-specific transport system in Escherichia coli but not for alkaline phosphatase repression. J Bacteriol. 1988;170:2283-6 pubmed
    The pstA gene encodes an integral membrane protein of the phosphate-specific transport system of Escherichia coli...
  8. Torriani A. From cell membrane to nucleotides: the phosphate regulon in Escherichia coli. Bioessays. 1990;12:371-6 pubmed
    ..phosphatase) by high Pi concentrations in the medium requires the presence of an intact Pst operon (pstS, pstC, pstA, pstB and phoU) and phoR...
  9. Rosenberg H, Gerdes R, Chegwidden K. Two systems for the uptake of phosphate in Escherichia coli. J Bacteriol. 1977;131:505-11 pubmed
    ..In strains in which both systems were fully operative, the initial rates of uptake was nearly additive, and the systems appeared to interact with a common intracellular phosphate pool. ..

Scientific Experts

More Information


  1. Aguena M, Spira B. RT-PCR of long prokaryotic operon transcripts without DNase treatment. J Microbiol Methods. 2003;55:419-23 pubmed
    ..To avoid amplification of genomic DNA, the hybrid-primer molecules that were not incorporated into the first-strand cDNA were removed by RNase H treatment followed by ultrafiltration...
  2. Rosen B, Liu Z. Transport pathways for arsenic and selenium: a minireview. Environ Int. 2009;35:512-5 pubmed publisher
    ..In this review we discuss the membrane proteins that transport arsenic and selenium into cells, from bacteria to humans, as well as some of the efflux proteins involved in detoxification. ..
  3. Amemura M, Makino K, Shinagawa H, Kobayashi A, Nakata A. Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli. J Mol Biol. 1985;184:241-50 pubmed
    The pstA(=phoT), pstB and phoU genes are situated at 84 minutes on the Escherichia coli genetic map...
  4. Surin B, Rosenberg H, Cox G. Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships. J Bacteriol. 1985;161:189-98 pubmed
    ..The complete sequence is transcribed counterclockwise, in the order phoS pstC pstA pstB phoU...
  5. Magota K, Otsuji N, Miki T, Horiuchi T, Tsunasawa S, Kondo J, et al. Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli. J Bacteriol. 1984;157:909-17 pubmed
    ..the nucleotide sequence of the regulatory region of phoS was compared with those of phoA and phoE, the genes constituting the pho regulon. ..
  6. Steed P, Wanner B. Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon. J Bacteriol. 1993;175:6797-809 pubmed
    ..Further, our results contradict those of M. Muda, N. N. Rao, and A. Torriani (J. Bacteriol. 174:8057-8064, 1992), who reported that the PhoU protein was required for Pi uptake. ..
  7. Drew D, Sj strand D, Nilsson J, Urbig T, Chin C, de Gier J, et al. Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc Natl Acad Sci U S A. 2002;99:2690-5 pubmed publisher
    ..We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA...
  8. Aguena M, Yagil E, Spira B. Transcriptional analysis of the pst operon of Escherichia coli. Mol Genet Genomics. 2002;268:518-24 pubmed
    ..of Escherichia coli, which encodes the phosphate-specific transport system, is composed of five genes, pstS, pstC, pstA, pstB and phoU, whose transcription is induced by phosphate starvation...