prmA

Summary

Gene Symbol: prmA
Description: methyltransferase for 50S ribosomal subunit protein L11
Alias: ECK3246, JW3227, prm-1, yhdI
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Colson C, Lhoest J, Urlings C. Genetics of ribosomal protein methylation in Escherichia coli. III. Map position of two genes, prmA and prmB, governing methylation of proteins L11 and L3. Mol Gen Genet. 1979;169:245-50 pubmed
    ..Gene prmA, governing methylation of protein L11 is situated at minute 71 on the map and is cotransduced with aroE (30%) and ..
  2. Jerez C, Weissbach H. Methylation of newly synthesized ribosomal protein L11 in a DNA-directed in vitro system. J Biol Chem. 1980;255:8706-10 pubmed
    ..lambda rifd 18 DNA also contains the genes for rRNA and newly synthesized 16 S and 23 S RNA are also methylated in this in vitro system. ..
  3. Dognin M, Wittmann Liebold B. Identification of methylated amino acids during sequence analysis. Application to the Escherichia coli ribosomal protein L11. Hoppe Seylers Z Physiol Chem. 1980;361:1697-705 pubmed
    ..The identification was confirmed by comparing the N-terminal dipeptide of protein L11 with the synthesised dipeptide Me3Ala-Lys. The behaviour of these methylated amino acids during sequence analysis is described. ..
  4. Colson C. Genetics of ribosomal protein methylation in Escherichia coli. I. A mutant deficient in methylation of protein L11. Mol Gen Genet. 1977;154:167-73 pubmed
    ..Since this mutant is fully viable, its methylation deficiency does not result in a major defect in ribosome assembly or functioning. ..
  5. Lederer F, Alix J, Hayes D. N-Trimethylalanine, a novel blocking group, found in E. coli ribosomal protein L11. Biochem Biophys Res Commun. 1977;77:470-80 pubmed
  6. Jackowski S, Alix J. Cloning, sequence, and expression of the pantothenate permease (panF) gene of Escherichia coli. J Bacteriol. 1990;172:3842-8 pubmed
    ..The predicted topological profile of pantothenate permease is similar to that of other membrane carriers that catalyze cation-dependent symport. ..
  7. Demirci H, Gregory S, Dahlberg A, Jogl G. Multiple-site trimethylation of ribosomal protein L11 by the PrmA methyltransferase. Structure. 2008;16:1059-66 pubmed
    ..In Escherichia coli, the lysine methyltransferase PrmA trimethylates the N-terminal alpha-amino group and the epsilon-amino groups of Lys3 and Lys39...
  8. Chang F, Cohen L, Navickas I, Chang C. Purification and properties of a ribosomal protein methylase from Eschericha coli Q13. Biochemistry. 1975;14:4994-8 pubmed
    ..Free ribosomal proteins were much better substrates for the methylation, indicating that methylation of 50S ribosomal proteins can occur before the complete assembly of the 50S ribosomal subunit. ..
  9. Vanet A, Plumbridge J, Alix J. Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12. J Bacteriol. 1993;175:7178-88 pubmed
    ..DNA region between panF, which encodes pantothenate permease, and orf1, the first gene of the fis operon, encodes prmA, the genetic determinant for the ribosomal protein L11 methyltransferase...

More Information

Publications15

  1. Bujnicki J. Sequence, structural, and evolutionary analysis of prokaryotic ribosomal protein L11 methyltransferases. Acta Microbiol Pol. 2000;49:19-29 pubmed
    The Escherichia coli PrmA enzyme catalyzes methylation of the large ribosomal subunit protein L11...
  2. Bausch S, Poliakova E, Draper D. Interactions of the N-terminal domain of ribosomal protein L11 with thiostrepton and rRNA. J Biol Chem. 2005;280:29956-63 pubmed
    ..These measurements also show that it is plausible for thiostrepton to compete directly with EF-G.GDP for binding to the L11-RNA complex, and provide a quantitative basis for further studies of L11 function and thiostrepton mechanism. ..
  3. Demirci H, Gregory S, Dahlberg A, Jogl G. Recognition of ribosomal protein L11 by the protein trimethyltransferase PrmA. EMBO J. 2007;26:567-77 pubmed publisher
    ..ribosomal protein L11 is post-translationally trimethylated at multiple residues by a single methyltransferase, PrmA. Here, we describe four structures of PrmA from the extreme thermophile Thermus thermophilus...
  4. Ball C, Osuna R, Ferguson K, Johnson R. Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli. J Bacteriol. 1992;174:8043-56 pubmed
    ..We suggest that the fluctuations in Fis levels may serve as an early signal of a nutritional upshift and may be important in the physiological roles Fis plays in the cell. ..
  5. Dognin M, Wittmann Liebold B. Purification and primary structure determination of the N-terminal blocked protein, L11, from Escherichia coli ribosomes. Eur J Biochem. 1980;112:131-51 pubmed
    ..The protein contains 141 amino acid residues and has a molecular weight of 14874. Secondary structure predictions of the protein are given, and its sequence is compared with those of other E. coli ribosomal proteins. ..
  6. Vanet A, Plumbridge J, Guerin M, Alix J. Ribosomal protein methylation in Escherichia coli: the gene prmA, encoding the ribosomal protein L11 methyltransferase, is dispensable. Mol Microbiol. 1994;14:947-58 pubmed
    The prmA gene, located at 72 min on the Escherichia coli chromosome, is the genetic determinant of ribosomal protein L11-methyltransferase activity...