priC

Summary

Gene Symbol: priC
Description: primosomal replication protein N''
Alias: ECK0461, JW0456
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Sandler S, Marians K, Zavitz K, Coutu J, Parent M, Clark A. dnaC mutations suppress defects in DNA replication- and recombination-associated functions in priB and priC double mutants in Escherichia coli K-12. Mol Microbiol. 1999;34:91-101 pubmed
    PriA, PriB and PriC were originally discovered as proteins essential for the PhiX174 in vitro DNA replication system...
  2. Sandler S. Multiple genetic pathways for restarting DNA replication forks in Escherichia coli K-12. Genetics. 2000;155:487-97 pubmed
    In Escherichia coli, the primosome assembly proteins, PriA, PriB, PriC, DnaT, DnaC, DnaB, and DnaG, are thought to help to restart DNA replication forks at recombinational intermediates...
  3. Ng J, Marians K. The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes. J Biol Chem. 1996;271:15642-8 pubmed
    ..As described in an accompanying article (Ng, J. Y., and Marians, K. J. (1996) J. Biol. Chem. 271, 15649-15655), when assembled on intact phiX174 phage DNA, the primosome also contains PriC.
  4. Sandler S, McCool J, Do T, Johansen R. PriA mutations that affect PriA-PriC function during replication restart. Mol Microbiol. 2001;41:697-704 pubmed
    ..The replication restart proteins, PriA, PriB, PriC, DnaB, DnaC, DnaG, DnaT and Rep, form redundant pathways that recognize repaired replication forks and restart them...
  5. Kim S, Pytlos M, Sinden R. Replication restart: a pathway for (CTG).(CAG) repeat deletion in Escherichia coli. Mutat Res. 2006;595:5-22 pubmed
    ..Mutations in priB and priC resulted in small reductions in deletion rates...
  6. Zavitz K, DiGate R, Marians K. The priB and priC replication proteins of Escherichia coli. Genes, DNA sequence, overexpression, and purification. J Biol Chem. 1991;266:13988-95 pubmed
    ..the role of these proteins in vivo, the genes for primosomal proteins n and n", designated priB and priC, respectively, have been cloned molecularly. priB encodes a 104-amino acid 11...
  7. Heller R, Marians K. The disposition of nascent strands at stalled replication forks dictates the pathway of replisome loading during restart. Mol Cell. 2005;17:733-43 pubmed
    ..Here, we provide biochemical evidence for an alternate system for DnaB loading that requires only PriC. Furthermore, the choice of which system is utilized during restart is dictated by the nature of the structure of ..
  8. Hinds T, Sandler S. Allele specific synthetic lethality between priC and dnaAts alleles at the permissive temperature of 30 degrees C in E. coli K-12. BMC Microbiol. 2004;4:47 pubmed
    ..PriA, PriB, PriC, DnaT, Rep and DnaC form multiple pathways to restart repaired replication forks...
  9. Huang C, Huang C. DnaT is a PriC-binding protein. Biochem Biophys Res Commun. 2016;477:988-992 pubmed publisher
    DnaT and PriC are replication restart primosomal proteins required for re-initiating chromosomal DNA replication. DnaT is a component of the PriA-dependent primosome, while PriC belongs to the PriC-dependent primosome...

More Information

Publications18

  1. Wessel S, Marceau A, Massoni S, Zhou R, Ha T, Sandler S, et al. PriC-mediated DNA replication restart requires PriC complex formation with the single-stranded DNA-binding protein. J Biol Chem. 2013;288:17569-78 pubmed publisher
    ..Here, we define a direct interaction between PriC, a key Escherichia coli DNA replication restart protein, and the single-stranded DNA-binding protein (SSB), a ..
  2. Boonsombat R, Yeh S, Milne A, Sandler S. A novel dnaC mutation that suppresses priB rep mutant phenotypes in Escherichia coli K-12. Mol Microbiol. 2006;60:973-83 pubmed
    ..During replication restart in Escherichia coli, the PriA, PriB, PriC, DnaT and Rep proteins form multiple pathways that bind to repaired replication forks...
  3. Ng J, Marians K. The ordered assembly of the phiX174-type primosome. II. Preservation of primosome composition from assembly through replication. J Biol Chem. 1996;271:15649-55 pubmed
    ..complexes formed on single-stranded DNA-binding protein-coated phiX174 DNA by the combination of PriA, PriB, PriC, DnaT, DnaB, DnaC, and DnaG...
  4. Masai H, Nomura N, Kubota Y, Arai K. Roles of phi X174 type primosome- and G4 type primase-dependent primings in initiation of lagging and leading strand syntheses of DNA replication. J Biol Chem. 1990;265:15124-33 pubmed
    ..a phi X174 type primosome is assembled at these sequences from the purified proteins, n'(priA), n(priB), n"(priC), dnaT, dnaB, dnaC, and primase...
  5. Sandler S. Requirements for replication restart proteins during constitutive stable DNA replication in Escherichia coli K-12. Genetics. 2005;169:1799-806 pubmed
    ..shown that dnaA rnhA mutants using cSDR for initiation of their DNA replication additionally require priB, but not priC, for viability...
  6. Lovett S. Filling the gaps in replication restart pathways. Mol Cell. 2005;17:751-2 pubmed
    Recent work by clarifies two mechanisms of replication fork assembly in E. coli: in vitro, the presence of a leading nascent strand dictates a PriA/B DnaT-dependent mechanism, whereas a gapped fork specifies PriC-mediated restart.
  7. Heller R, Marians K. Unwinding of the nascent lagging strand by Rep and PriA enables the direct restart of stalled replication forks. J Biol Chem. 2005;280:34143-51 pubmed
    During origin-independent replisome assembly, the replication restart protein PriC prefers to load the replication fork helicase, DnaB, to stalled replication forks where there is a gap in the nascent leading strand...
  8. Flores M, Ehrlich S, Michel B. Primosome assembly requirement for replication restart in the Escherichia coli holDG10 replication mutant. Mol Microbiol. 2002;44:783-92 pubmed
    ..In contrast, priC inactivation has no effect...
  9. Allen G, Kornberg A. Assembly of the primosome of DNA replication in Escherichia coli. J Biol Chem. 1993;268:19204-9 pubmed
    Assembly of the Escherichia coli primosome requires six proteins, PriA, PriB, PriC, DnaB, DnaC, and DnaT, acting at a primosome assembly site (pas) on an SSB-coated single-stranded (ss) DNA...