Genomes and Genes
Gene Symbol: ppiA
Description: peptidyl-prolyl cis-trans isomerase A (rotamase A)
Alias: ECK3351, JW3326, rot, rotA
Species: Escherichia coli str. K-12 substr. MG1655
- Konno M, Ito M, Hayano T, Takahashi N. The substrate-binding site in Escherichia coli cyclophilin A preferably recognizes a cis-proline isomer or a highly distorted form of the trans isomer. J Mol Biol. 1996;256:897-908 pubmed..Thus, the extra amino acid residue of E. coli CyPA, polar Gln89, lies along the pathway to the hydrophobic pocket of CyPA and seems to prevent the access hydrophobic part of CsA to the cleft of CyPA. ..
- Tran P, Bannor T, Doktor S, Nichols B. Chromosomal organization and expression of Escherichia coli pabA. J Bacteriol. 1990;172:397-410 pubmed..The significance of these results and possible posttranscriptional control mechanisms which affect PabA expression from the P2-initiated polycistronic unit are discussed. ..
- Kawamukai M, Matsuda H, Fujii W, Utsumi R, Komano T. Nucleotide sequences of fic and fic-1 genes involved in cell filamentation induced by cyclic AMP in Escherichia coli. J Bacteriol. 1989;171:4525-9 pubmed..coli. In addition, ORF190 has sequence similarity with the cyclosporin A-binding protein cyclophilin. ..
- Clubb R, Ferguson S, Walsh C, Wagner G. Three-dimensional solution structure of Escherichia coli periplasmic cyclophilin. Biochemistry. 1994;33:2761-72 pubmed..A residue essential for isomerase activity in human T cell cyclophilin (His126) is replaced by Tyr122 in E. coli cyclophilin without affecting enzymatic activity. ..
- Compton L, Davis J, MacDonald J, Bachinger H. Structural and functional characterization of Escherichia coli peptidyl-prolyl cis-trans isomerases. Eur J Biochem. 1992;206:927-34 pubmed..Circular dichroism spectroscopy indicates that the secondary structure of the cationic protein consists of 17% alpha-helix, 34% beta-sheet, 17% turns, 33% random coil and is very similar to human cytosolic PPIase. ..
- Liu J, Walsh C. Peptidyl-prolyl cis-trans-isomerase from Escherichia coli: a periplasmic homolog of cyclophilin that is not inhibited by cyclosporin A. Proc Natl Acad Sci U S A. 1990;87:4028-32 pubmed..The E. coli rotamase, a product of the gene we suggest be called "rot," has been purified to homogeneity after cloning of the gene by the polymerase chain reaction and its ..