Gene Symbol: pitA
Description: phosphate transporter, low-affinity; tellurite importer
Alias: ECK3478, JW3460, pit
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Harris R, Webb D, Howitt S, Cox G. Characterization of PitA and PitB from Escherichia coli. J Bacteriol. 2001;183:5008-14 pubmed
    ..The low-affinity P(i) transporter (pitA) is expressed constitutively and is dependent on the proton motive force, while the high-affinity Pst system (..
  2. Sprague G, Bell R, Cronan J. A mutant of Escherichia coli auxotrophic for organic phosphates: evidence for two defects in inorganic phosphate transport. Mol Gen Genet. 1975;143:71-7 pubmed
    ..One lesion, pst, is located at minute 74 of the E. coli genetic map while the other lesion, pit, is located at minute 68. All "K10" strains that were examined carry the pit lesion...
  3. Elvin C, Dixon N, Rosenberg H. Molecular cloning of the phosphate (inorganic) transport (pit) gene of Escherichia coli K12. Identification of the pit+ gene product and physical mapping of the pit-gor region of the chromosome. Mol Gen Genet. 1986;204:477-84 pubmed
    The pit+ gene, encoding the phosphate (inorganic) transport system of Escherichia coli, was isolated from a library of E. coli genes inserted in the cosmid vector pHC79. A 25...
  4. Beard S, Hashim R, Wu G, Binet M, Hughes M, Poole R. Evidence for the transport of zinc(II) ions via the pit inorganic phosphate transport system in Escherichia coli. FEMS Microbiol Lett. 2000;184:231-5 pubmed
    ..The mutation was located within the pitA gene, which encodes the low-affinity inorganic phosphate transport system (Pit)...
  5. Peterson C, Mandel M, Silhavy T. Escherichia coli starvation diets: essential nutrients weigh in distinctly. J Bacteriol. 2005;187:7549-53 pubmed
  6. Elvin C, Hardy C, Rosenberg H. Pi exchange mediated by the GlpT-dependent sn-glycerol-3-phosphate transport system in Escherichia coli. J Bacteriol. 1985;161:1054-8 pubmed
    ..Glucose and other phosphotransferase system sugars inhibit Pi efflux by repressing glpT expression. ..
  7. Willsky G, Malamy M. Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli. J Bacteriol. 1980;144:356-65 pubmed
    ..However, cells dependent upon the low affinity-high velocity Pit (Pi transport) system have a Km of 38.2 +/- 0.4 microM and a Vmax of 55 +/- 1...
  8. Willsky G, Malamy M. Effect of arsenate on inorganic phosphate transport in Escherichia coli. J Bacteriol. 1980;144:366-74 pubmed
    ..Arsenate accumulation required a metabolizable energy source and was inhibited by N-ethylmaleimide. Previously accumulated arsenate could exchange with arsenate or Pi in the medium. ..
  9. Konings W, Rosenberg H. Phosphate transport in membrane vesicles from Escherichia coli. Biochim Biophys Acta. 1978;508:370-8 pubmed
    Escherichia coli strain AN710 possesses only the PIT system for phosphate transport. Membrane vesicles from this strain, which contain phosphate internally, perform exchange and active transport of phosphate...

More Information


  1. Rao N, Torriani A. Molecular aspects of phosphate transport in Escherichia coli. Mol Microbiol. 1990;4:1083-90 pubmed
    Escherichia coli transports inorganic phosphate (Pi) by the low-affinity transport system, Pit. When the level of the external Pi is lower than 20 microM, another transport system, Pst, is induced with a Kt of 0.25 microM...
  2. Lee K, Kenney F. Induction of alanine transaminase by adrenal steroids in cultured hepatoma cells. Biochem Biophys Res Commun. 1970;40:469-75 pubmed
  3. Elías A, Abarca M, Montes R, Chasteen T, Pérez Donoso J, Vásquez C. Tellurite enters Escherichia coli mainly through the PitA phosphate transporter. Microbiologyopen. 2012;1:259-67 pubmed publisher
    ..Results showed that the PitA phosphate transporter was related to tellurite uptake...
  4. Elías A, Díaz Vásquez W, Abarca Lagunas M, Chasteen T, Arenas F, Vásquez C. The ActP acetate transporter acts prior to the PitA phosphate carrier in tellurite uptake by Escherichia coli. Microbiol Res. 2015;177:15-21 pubmed publisher
    ..While the PitA phosphate transporter is thought to be one of the main paths responsible for toxicant entry into Escherichia coli, ..
  5. Rosenberg H, Gerdes R, Chegwidden K. Two systems for the uptake of phosphate in Escherichia coli. J Bacteriol. 1977;131:505-11 pubmed
    ..A comparison of these strains showed that one of the systems (PIT) was fully constitutive, required no binding protein, and operated in spheroplasts...
  6. Hoffer S, Tommassen J. The phosphate-binding protein of Escherichia coli is not essential for P(i)-regulated expression of the pho regulon. J Bacteriol. 2001;183:5768-71 pubmed
    ..We demonstrate that P(i)-controlled expression is restored when the activity of the P(i) transporter PitA or PitB is increased. Apparently, PstS is not an essential component of the signal transduction pathway.
  7. Jackson R, Binet M, Lee L, Ma R, Graham A, McLeod C, et al. Expression of the PitA phosphate/metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels. FEMS Microbiol Lett. 2008;289:219-24 pubmed publisher
    ..The Pst system (pstSCAB) is inducible by low phosphate concentrations whereas the low-affinity transporter (pitA) has been described as constitutively expressed...