Gene Symbol: phnD
Description: phosphonate ABC transporter periplasmic binding protein
Alias: ECK4098, JW4066, psiD
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Rizk S, Cuneo M, Hellinga H. Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates. Protein Sci. 2006;15:1745-51 pubmed
    The Escherichia coli phnD gene is hypothesized to code for the periplasmic binding component of a phosphonate uptake system. Here we report the characterization of the phosphonate-binding properties of the phnD protein product...
  2. Metcalf W, Wanner B. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi. J Bacteriol. 1991;173:587-600 pubmed
    The phn (psiD) gene cluster is induced during Pi limitation and is required for the use of phosphonates (Pn) as a phosphorus (P) source...
  3. Wanner B, Metcalf W. Molecular genetic studies of a 10.9-kb operon in Escherichia coli for phosphonate uptake and biodegradation. FEMS Microbiol Lett. 1992;100:133-9 pubmed
    ..Three gene products (PhnC, PhnD and PhnE) comprise a binding protein-dependent phosphonate transporter, which also transports phosphate, phosphite, ..
  4. Wanner B, Boline J. Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli. J Bacteriol. 1990;172:1186-96 pubmed
    The Escherichia coli phn (psiD) locus encodes genes for phosphonate (Pn) utilization, for phn (psiD) mutations abolish the ability to use as a sole P source a Pn with a substituted C-2 or unsubstituted hydrocarbon group such as 2-..
  5. Chen C, Ye Q, Zhu Z, Wanner B, Walsh C. Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B. J Biol Chem. 1990;265:4461-71 pubmed
    ..sequences are found in the phn DNA: 10 exist in the extragenic region between phnA and phnB, two between phnD and phnE, and two between phnK and phnL...
  6. Metcalf W, Steed P, Wanner B. Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions. J Bacteriol. 1990;172:3191-200 pubmed
    ..Also, Mu d1s in the phoA and phn (psiD) loci of the phosphate regulon showed regional specificities for the insertion sites despite the randomness of Mu ..
  7. Iqbal S, Parker G, Davidson H, Moslehi Rahmani E, Robson R. Reversible phase variation in the phnE gene, which is required for phosphonate metabolism in Escherichia coli K-12. J Bacteriol. 2004;186:6118-23 pubmed
  8. Saurin W, Dassa E. Sequence relationships between integral inner membrane proteins of binding protein-dependent transport systems: evolution by recurrent gene duplications. Protein Sci. 1994;3:325-44 pubmed
    ..According to this model, it is probable that these transport systems derive from an ancestral system having only 1 hydrophobic membrane protein.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  9. Metcalf W, Wanner B. Mutational analysis of an Escherichia coli fourteen-gene operon for phosphonate degradation, using TnphoA' elements. J Bacteriol. 1993;175:3430-42 pubmed
    ..cluster is an operon of 14 genes, and the phnC promoter is the sole psi promoter; (ii) three gene products (PhnC, PhnD, and PhnE) probably constitute a binding protein-dependent Pn transporter; (iii) seven gene products (PhnG, PhnH, ..

Scientific Experts

More Information


  1. Elashvili I, DeFrank J, Culotta V. phnE and glpT genes enhance utilization of organophosphates in Escherichia coli K-12. Appl Environ Microbiol. 1998;64:2601-8 pubmed
    ..This result indicates that in E. coli, X-Pi is transported exclusively by the Phn system, whereas DIPP (or its metabolite) may be transported by both Phn and Glp systems. ..
  2. Yakovleva G, Kim S, Wanner B. Phosphate-independent expression of the carbon-phosphorus lyase activity of Escherichia coli. Appl Microbiol Biotechnol. 1998;49:573-8 pubmed
    ..coli cells, releasing the carbon moiety into the periplasm. These data are in agreement with the observation that phosphonates cannot serve as the carbon source for E. coli growth. ..
  3. Alicea I, Marvin J, Miklos A, Ellington A, Looger L, Schreiter E. Structure of the Escherichia coli phosphonate binding protein PhnD and rationally optimized phosphonate biosensors. J Mol Biol. 2011;414:356-69 pubmed publisher
    The phnD gene of Escherichia coli encodes the periplasmic binding protein of the phosphonate (Pn) uptake and utilization pathway. We have crystallized and determined structures of E...
  4. Hare R, Walker S, Dorman T, Greene J, Guzman L, Kenney T, et al. Genetic footprinting in bacteria. J Bacteriol. 2001;183:1694-706 pubmed
    ..In addition, the ability of recombinant proteins to complement mutagenized hosts has been evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system. ..
  5. Metcalf W, Wanner B. Evidence for a fourteen-gene, phnC to phnP locus for phosphonate metabolism in Escherichia coli. Gene. 1993;129:27-32 pubmed
    ..Also, these plasmids were employed to introduce nonpolar phnD::lacZ and phnD::uidA fusions into the chromosome, which allowed us to show that phnD probably has a role in ..
  6. Wackett L, Wanner B, Venditti C, Walsh C. Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12. J Bacteriol. 1987;169:1753-6 pubmed
    ..Since psiD::lacZ(Mu d1) mutants cannot metabolize methylphosphonic acid, psiD may be the structural gene(s) for C-P lyase.
  7. Makino K, Kim S, Shinagawa H, Amemura M, Nakata A. Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12. J Bacteriol. 1991;173:2665-72 pubmed
    ..Studies of the structure, regulation, and function of the phn region suggest that the phosphate starvation-inducible phn operon consists of 14 cistrons from phnC to phnP. ..
  8. Wanner B. Genes for phosphonate biodegradation in Escherichia coli. SAAS Bull Biochem Biotechnol. 1992;5:1-6 pubmed
    ..Fourteen genes for phosphonate metabolism comprise the phnC-to-phnP gene cluster: three gene products (PhnC, PhnD, and PhnE) comprise a binding protein-dependent phosphonate transporter, which also transports Pi and phosphate ..