Gene Symbol: phnC
Description: phosphonate ABC transporter ATPase
Alias: ECK4099, JW4067
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Wanner B, Metcalf W. Molecular genetic studies of a 10.9-kb operon in Escherichia coli for phosphonate uptake and biodegradation. FEMS Microbiol Lett. 1992;100:133-9 pubmed
    ..coli, which are organized in an operon of 14 genes, named phnC to phnP...
  2. Wanner B. Genes for phosphonate biodegradation in Escherichia coli. SAAS Bull Biochem Biotechnol. 1992;5:1-6 pubmed
    ..Fourteen genes for phosphonate metabolism comprise the phnC-to-phnP gene cluster: three gene products (PhnC, PhnD, and PhnE) comprise a binding protein-dependent phosphonate ..
  3. Makino K, Kim S, Shinagawa H, Amemura M, Nakata A. Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12. J Bacteriol. 1991;173:2665-72 pubmed
    ..An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping...
  4. Metcalf W, Wanner B. Mutational analysis of an Escherichia coli fourteen-gene operon for phosphonate degradation, using TnphoA' elements. J Bacteriol. 1993;175:3430-42 pubmed
    ..phosphonate (Pn) utilization in Escherichia coli are in a large cluster of 14 genes named, in alphabetical order, phnC to phnP...
  5. Yakovleva G, Kim S, Wanner B. Phosphate-independent expression of the carbon-phosphorus lyase activity of Escherichia coli. Appl Microbiol Biotechnol. 1998;49:573-8 pubmed
    ..9-kb operon of 14 genes (named phnC to phnP) and induced by phosphate (P(i)) starvation [Metcalf and Wanner (1993) J Bacteriol 175: 3430-3442]...
  6. Metcalf W, Wanner B. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi. J Bacteriol. 1991;173:587-600 pubmed
    ..We propose a smaller gene cluster with 14 open reading frames, phnC to phnP, which probably encode transporter and regulatory functions, in addition to proteins needed in Pn ..
  7. Metcalf W, Steed P, Wanner B. Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions. J Bacteriol. 1990;172:3191-200 pubmed
    ..psiE and psiF::lacZ(Mu d1)s lie in uncharacterized open reading frames near the xylE and phoA genes, respectively. Six other psi::lacZ(Mu d1)s lie in yet unreported Escherichia coli sequences. ..
  8. Wackett L, Wanner B, Venditti C, Walsh C. Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12. J Bacteriol. 1987;169:1753-6 pubmed
    ..Since psiD::lacZ(Mu d1) mutants cannot metabolize methylphosphonic acid, psiD may be the structural gene(s) for C-P lyase. ..
  9. Metcalf W, Wanner B. Evidence for a fourteen-gene, phnC to phnP locus for phosphonate metabolism in Escherichia coli. Gene. 1993;129:27-32 pubmed
    ..This was done by testing the effect of phn::uidA insertions in or near the 3' end of phnP on Pn use. Altogether, these results show that all genes required for Pn use are in the 10.9-kb, fourteen-gene, phnCDEFGHIJKLMNOP locus. ..

More Information


  1. Elashvili I, DeFrank J, Culotta V. phnE and glpT genes enhance utilization of organophosphates in Escherichia coli K-12. Appl Environ Microbiol. 1998;64:2601-8 pubmed
    ..This result indicates that in E. coli, X-Pi is transported exclusively by the Phn system, whereas DIPP (or its metabolite) may be transported by both Phn and Glp systems. ..
  2. Iqbal S, Parker G, Davidson H, Moslehi Rahmani E, Robson R. Reversible phase variation in the phnE gene, which is required for phosphonate metabolism in Escherichia coli K-12. J Bacteriol. 2004;186:6118-23 pubmed
  3. Rizk S, Cuneo M, Hellinga H. Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates. Protein Sci. 2006;15:1745-51 pubmed
    ..Since MP is the final degradation product of many nerve agents, these PhnD conjugates can function as components in a biosensor system for chemical warfare agents. ..
  4. Wanner B, Boline J. Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli. J Bacteriol. 1990;172:1186-96 pubmed
    ..However, no gross structural change was detected in independent Phn+ phn(EcoK) mutants that have activating mutations near the phn locus. ..
  5. Chen C, Ye Q, Zhu Z, Wanner B, Walsh C. Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B. J Biol Chem. 1990;265:4461-71 pubmed
    ..Sequence homology searches identify PhnC, PhnK, PhnL, and, possibly, PhnN proteins as members of nucleotide-binding proteins of the binding protein-..
  6. Saurin W, Dassa E. Sequence relationships between integral inner membrane proteins of binding protein-dependent transport systems: evolution by recurrent gene duplications. Protein Sci. 1994;3:325-44 pubmed
    ..According to this model, it is probable that these transport systems derive from an ancestral system having only 1 hydrophobic membrane protein.(ABSTRACT TRUNCATED AT 250 WORDS) ..