Gene Symbol: pheM
Description: phenylalanyl-tRNA synthetase operon leader peptide
Alias: ECK1713, JW1705, phtL
Species: Escherichia coli str. K-12 substr. MG1655
Wertheimer S, Klotsky R, Schwartz I. Transcriptional patterns for the thrS-infC-rplT operon of Escherichia coli. Gene. 1988;63:309-20 pubmed
Springer M, Graffe M, Mayaux J, Dardel F, Fayat G, Blanquet S, et al
. Open reading frames in the control regions of the phenylalanyl-tRNA synthetase operon of E. coli. Biochimie. 1987;69:1065-70 pubmed
..The present report shows that, in fact, the only open reading frame to be translated efficiently is the leader peptide itself. The alternative leader peptide and the terminator peptide are both translated at a negligible rate. ..
Mayaux J, Fayat G, Panvert M, Springer M, Grunberg Manago M, Blanquet S. Control of phenylalanyl-tRNA synthetase genetic expression. Site-directed mutagenesis of the pheS, T operon regulatory region in vitro. J Mol Biol. 1985;184:31-44 pubmed
Mayaux J, Springer M, Graffe M, Fromant M, Fayat G. IS4 transposition in the attenuator region of the Escherichia coli pheS,T operon. Gene. 1984;30:137-46 pubmed
..171 (1983) 239-261; Springer et al., J. Mol. Biol. 171 (1983) 263-279]. The IS4 insertion site described here is compared to the other known sites and the effect of IS4 transposition on the expression of neighbouring genes is discussed. ..
Fayat G, Mayaux J, Sacerdot C, Fromant M, Springer M, Grunberg Manago M, et al
. Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20. J Mol Biol. 1983;171:239-61 pubmed
..Further evidence is provided for this model by the features of pheS, T regulation in vivo (see the accompanying paper). ..
Springer M, Trudel M, Graffe M, Plumbridge J, Fayat G, Mayaux J, et al
. Escherichia coli phenylalanyl-tRNA synthetase operon is controlled by attenuation in vivo. J Mol Biol. 1983;171:263-79 pubmed
..indicate that phenylalanyl-tRNA synthetase is controlled by attenuation in a way analogous to several amino acid biosynthetic operons. ..
Trudel M, Springer M, Graffe M, Fayat G, Blanquet S, Grunberg Manago M. Regulation of E.coli phenylalanyl-tRNA synthetase operon in vivo. Biochim Biophys Acta. 1984;782:10-7 pubmed
..By analogy with other well-studied amino acid biosynthetic operons known to be controlled by attenuation, these in vivo results indicate that phenylalanyl-tRNA synthetase levels are controlled by an attenuation-like mechanism. ..
Mechulam Y, Fayat G, Blanquet S. Sequence of the Escherichia coli pheST operon and identification of the himA gene. J Bacteriol. 1985;163:787-91 pubmed
..Another open reading frame (prp) was revealed downstream from pheT which was identified as himA, the gene for the alpha subunit of the integration host factor. ..
Springer M, Mayaux J, Fayat G, Plumbridge J, Graffe M, Blanquet S, et al
. Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon. J Mol Biol. 1985;181:467-78 pubmed
..The pheST operon is also shown to be derepressed by a cellular excess of phenylalanyl-tRNA synthetase. This derepression is shown to be due to the pheST attenuator. ..