Gene Symbol: panC
Description: pantothenate synthetase
Alias: ECK0132, JW0129
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Miyatake K, Nakano Y, Kitaoka S. Pantothenate synthetase of Escherichia coli B. I. Physicochemical properties. J Biochem. 1976;79:673-8 pubmed
    ..71 cm3/g. The enzyme had an amino-terminal glycyl residue and a Leu-Ala-Ser-OH sequence at the carboxyl end. Electrophoresis of the enzyme with carrier ampholine gave an isoelectric point of pH 4.6. ..
  2. Vallari D, Rock C. Isolation and characterization of Escherichia coli pantothenate permease (panF) mutants. J Bacteriol. 1985;164:136-42 pubmed
    ..The growth phenotypes of panF panD, panF panB, and panF panC double mutants showed that the cytoplasmic membrane was impermeable to external pantothenate...
  3. Maas W, Novelli G. Synthesis of pantothenic acid by depyrophosphorylation of adenosine triphosphate. Arch Biochem Biophys. 1953;43:236-8 pubmed
  4. Tuck K, Saldanha S, Birch L, Smith A, Abell C. The design and synthesis of inhibitors of pantothenate synthetase. Org Biomol Chem. 2006;4:3598-610 pubmed
    ..The esters were all modest competitive inhibitors, the sulfamoyls were more potent, consistent with their closer structural similarity to the pantoyl adenylate intermediate. ..
  5. Hauser R, Ceol A, Rajagopala S, Mosca R, Siszler G, Wermke N, et al. A second-generation protein-protein interaction network of Helicobacter pylori. Mol Cell Proteomics. 2014;13:1318-29 pubmed publisher
    ..coli of which one third turned out to be conserved in both species. ..
  6. Miyatake K, Nakano Y, Kitaoka S. Enzymological properties of pantothenate synthetase from Escherichia coli B. J Nutr Sci Vitaminol (Tokyo). 1978;24:243-53 pubmed
    ..0 x 10(-4)M. According to the method of Cleland, the enzyme reaction proceeds by a Bi Uni Uni Bi Ping Pong mechanism and a scheme showing the order of binding of substrates and releasing of products is presented. ..
  7. Jones C, Brook J, Buck D, Abell C, Smith A. Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme. J Bacteriol. 1993;175:2125-30 pubmed
    ..Northern (RNA) analysis showed that the panB gene is likely to be cotranscribed with at least one other gene but that this is not the putative fimbrial protein, since no transcripts for this gene could be detected. ..
  8. von Delft F, Lewendon A, Dhanaraj V, Blundell T, Abell C, Smith A. The crystal structure of E. coli pantothenate synthetase confirms it as a member of the cytidylyltransferase superfamily. Structure. 2001;9:439-50 pubmed
    ..This relationship has been used to suggest the location of the ATP and pantoate binding sites and the nature of hinge bending that leads to the ternary enzyme-pantoate-ATP complex. ..
  9. Tao D, Zhu G, Sun L, Ma J, Liang Z, Zhang W, et al. Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis. Proteomics. 2009;9:2029-36 pubmed publisher
    ..All these results demonstrated that serially couple microcolumns might be of great promising to improve the separation capacity of microRPLC in shotgun proteomic analysis. ..

More Information


  1. Chakrabarti K, Thakur K, Gopal B, Sarma S. X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate. FEBS J. 2010;277:697-712 pubmed publisher
    ..The results presented here have implications for the design and development of potential antibacterial and herbicidal agents. ..
  2. Miyatake K, Nakano Y, Kitaoka S. Pantothenate synthetase from Escherichia coli [D-pantoate: beta-alanine ligase (AMP-forming), EC]. Methods Enzymol. 1979;62:215-9 pubmed
  3. Merkel W, Nichols B. Characterization and sequence of the Escherichia coli panBCD gene cluster. FEMS Microbiol Lett. 1996;143:247-52 pubmed
    ..panB, panC, and panD were identified by subcloning and insertional mutagenesis...
  4. Maas W. Pantothenate studies. III. Description of the extracted pantothenate-synthesizing enzyme of Escherichia coli. J Biol Chem. 1952;198:23-32 pubmed
  5. Chakrabarti K, Sarma S. NMR assignment of 2H, 13C and 15N labeled amino-terminal domain of apo-pantothenate synthetase from E. coli. J Biomol NMR. 2006;36 Suppl 1:38 pubmed
  6. Cronan J, Littel K, Jackowski S. Genetic and biochemical analyses of pantothenate biosynthesis in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1982;149:916-22 pubmed
    ..typhimurium are deficient in ketopantoate hydroxymethyltransferase, whereas the panC mutants lack pantothenate synthetase. panD mutants of E...