Gene Symbol: panB
Description: 3-methyl-2-oxobutanoate hydroxymethyltransferase
Alias: ECK0133, JW0130, panA
Species: Escherichia coli str. K-12 substr. MG1655
Powers S, Snell E. Ketopantoate hydroxymethyltransferase. II. Physical, catalytic, and regulatory properties. J Biol Chem. 1976;251:3786-93 pubmed
..Catalytic activity of the transferase is thus regulated by the products of the reaction path of which it is one component; transferase synthesis is not repressed by growth in the presence of pantothenate. ..
Ericsson U, Hallberg B, DeTitta G, Dekker N, Nordlund P. Thermofluor-based high-throughput stability optimization of proteins for structural studies. Anal Biochem. 2006;357:289-98 pubmed
..This suggests that thermofluor constitutes an efficient generic high-throughput method for identification of protein properties predictive of crystallizability. ..
von Delft F, Inoue T, Saldanha S, Ottenhof H, Schmitzberger F, Birch L, et al
. Structure of E. coli ketopantoate hydroxymethyl transferase complexed with ketopantoate and Mg2+, solved by locating 160 selenomethionine sites. Structure. 2003;11:985-96 pubmed
..The active site contains a ketopantoate bidentately coordinated to Mg(2+). Similar binding is likely for the substrate, alpha-ketoisovalerate, orienting the C3 for deprotonation. ..
Jones C, Brook J, Buck D, Abell C, Smith A. Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme. J Bacteriol. 1993;175:2125-30 pubmed
The panB gene from Escherichia coli, encoding the first enzyme of the pantothenate biosynthesis pathway, ketopantoate hydroxymethyltransferase (KPHMT), has been isolated by functional complementation of a panB mutant strain with an E...
Park J, Lee K, Kim T, Lee S. Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation. Proc Natl Acad Sci U S A. 2007;104:7797-802 pubmed
..The ilvA, leuA, and panB genes were deleted to make more precursors available for L-valine biosynthesis...
Merkel W, Nichols B. Characterization and sequence of the Escherichia coli panBCD gene cluster. FEMS Microbiol Lett. 1996;143:247-52 pubmed
..b>panB, panC, and panD were identified by subcloning and insertional mutagenesis...
Schmitzberger F, Smith A, Abell C, Blundell T. Comparative analysis of the Escherichia coli ketopantoate hydroxymethyltransferase crystal structure confirms that it is a member of the (betaalpha)8 phosphoenolpyruvate/pyruvate superfamily. J Bacteriol. 2003;185:4163-71 pubmed
..Based on the analysis, we propose that in this superfamily there should be a subdivision into two groups. This paper completes our structural analysis of the E. coli enzymes in the pantothenate pathway. ..
Cronan J, Littel K, Jackowski S. Genetic and biochemical analyses of pantothenate biosynthesis in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1982;149:916-22 pubmed
..The panB mutants of both organisms and the pan-6 ("panA") mutant of S...
Levy E, Boeri Erba E, Robinson C, Teichmann S. Assembly reflects evolution of protein complexes. Nature. 2008;453:1262-5 pubmed publisher
..Our model of self-assembly allows reliable prediction of evolution and assembly of a complex solely from its crystal structure. ..