Gene Symbol: pabA
Description: aminodeoxychorismate synthase, subunit II
Alias: ECK3348, JW3323
Species: Escherichia coli str. K-12 substr. MG1655
Roux B, Walsh C. p-aminobenzoate synthesis in Escherichia coli: kinetic and mechanistic characterization of the amidotransferase PabA. Biochemistry. 1992;31:6904-10 pubmed
p-Aminobenzoic acid (PABA) is an important precursor in the bacterial biosynthetic pathway for folate enzymes. This biosynthesis requires three separate proteins: PabA, PabB, and PabC...
Bruce I, Hardy J, Stacey K. Potentiation by purines of the growth-inhibitory effects of sulphonamides on Escherichia coli K12 and the location of the gene which mediates this effect. J Gen Microbiol. 1984;130:2489-95 pubmed
..This result suggests that these lysogens are heterozygous for psp and that the wild-type allele is dominant. It is probable that psp is a regulatory gene, affecting some rate-limiting step in the biosynthesis of methionine. ..
Huang M, Gibson F. Biosynthesis of 4-aminobenzoate in Escherichia coli. J Bacteriol. 1970;102:767-73 pubmed
Two different mutations (pabA and pabB) affecting 4-aminobenzoate biosynthesis were obtained in strains of Escherichia coli lacking chorismate mutase and anthranilate synthetase activity, thus allowing study of the pathway of biosynthesis ..
Kawamukai M, Matsuda H, Fujii W, Utsumi R, Komano T. Nucleotide sequences of fic and fic-1 genes involved in cell filamentation induced by cyclic AMP in Escherichia coli. J Bacteriol. 1989;171:4525-9 pubmed
..The promoter activity of fic was confirmed by fusing fic and lacZ. The gene downstream from fic was found to be pabA (p-aminobenzoate). There is an open reading frame (ORF190) coding for 190 amino acids upstream from the fic gene...
Ye Q, Liu J, Walsh C. p-Aminobenzoate synthesis in Escherichia coli: purification and characterization of PabB as aminodeoxychorismate synthase and enzyme X as aminodeoxychorismate lyase. Proc Natl Acad Sci U S A. 1990;87:9391-5 pubmed
The Escherichia coli pabA and pabB genes have been overexpressed separately and in tandem...
Walsh C, Erion M, Walts A, Delany J, Berchtold G. Chorismate aminations: partial purification of Escherichia coli PABA synthase and mechanistic comparison with anthranilate synthase. Biochemistry. 1987;26:4734-45 pubmed
Chorismate is converted by regiospecific amination/aromatization sequences to o-aminobenzoate and p-aminobenzoate (PABA) by anthranilate synthase (AS) and PABA synthase (PABS), respectively...
Kaplan J, Nichols B. Nucleotide sequence of Escherichia coli pabA and its evolutionary relationship to trp(G)D. J Mol Biol. 1983;168:451-68 pubmed
We have determined the entire nucleotide sequence of Escherichia coli pabA. A comparison of the nucleotide and amino acid sequences of pabA and trp(G) D reveals extensive homology, suggesting that these two genes arose from a common ..
Viswanathan V, Green J, Nichols B. Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli. J Bacteriol. 1995;177:5918-23 pubmed
The metabolic fate of p-aminobenzoic acid (PABA) in Escherichia coli is its incorporation into the vitamin folic acid. PABA is derived from the aromatic branch point precursor chorismate in two steps...
Tran P, Bannor T, Doktor S, Nichols B. Chromosomal organization and expression of Escherichia coli pabA. J Bacteriol. 1990;172:397-410 pubmed
The pabA gene in Escherichia coli and Salmonella typhimurium encodes the glutamine amidotransferase subunit of para-aminobenzoate synthase, which catalyzes the first reaction in the conversion of chorismate to para-aminobenzoate (PABA)...