Gene Symbol: omsA
Description: UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase
Alias: ECK0178, JW0174, firA, omsA, ssc
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Vaara M, Nurminen M. Outer membrane permeability barrier in Escherichia coli mutants that are defective in the late acyltransferases of lipid A biosynthesis. Antimicrob Agents Chemother. 1999;43:1459-62 pubmed
    ..These findings indicate that hexa-acylated lipid A is not a prerequisite for the normal function of the outer membrane permeability barrier. ..
  2. Trent M. Biosynthesis, transport, and modification of lipid A. Biochem Cell Biol. 2004;82:71-86 pubmed
  3. Platko J, Willins D, Calvo J. The ilvIH operon of Escherichia coli is positively regulated. J Bacteriol. 1990;172:4563-70 pubmed
    ..We propose that the designation lrp be used in the future instead of oppI or ihb and that Lrp (leucine-responsive regulatory protein) be used in place of IHB. ..
  4. Raetz C, Reynolds C, Trent M, Bishop R. Lipid A modification systems in gram-negative bacteria. Annu Rev Biochem. 2007;76:295-329 pubmed
    ..Although not required for growth, the modification enzymes modulate virulence of some gram-negative pathogens. Heterologous expression of lipid A modification enzymes may enable the development of new vaccines. ..
  5. Plesiat P, Vaara M. Outer membrane permeability of the antibiotic-supersusceptible lipid A mutants of Escherichia coli to hydrophobic steroid probes. J Antimicrob Chemother. 1999;43:608-10 pubmed
  6. Lathe R, Lecocq J. The firA gene, a locus involved in the expression of rifampicin resistance in Escherichia coli. I. Characterisation of lambdafirA transducing phages constructed in vitro. Mol Gen Genet. 1977;154:43-51 pubmed
    ..A priori, the firA gene is likely to code for an essential component of the transcription apparatus...
  7. Kim H, Lee C, Kim M, Peterkofsky A, Seok Y. Dephosphorylated NPr of the nitrogen PTS regulates lipid A biosynthesis by direct interaction with LpxD. Biochem Biophys Res Commun. 2011;409:556-61 pubmed publisher
    ..Taken together, our data suggest that unphosphorylated NPr decreases lipid A biosynthesis by inhibiting LpxD activity. ..
  8. Dicker I, Seetharam S. Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J Bacteriol. 1991;173:334-44 pubmed
    The Escherichia coli gene firA, previously reported to code for a small, histonelike DNA-binding protein, has been cloned and found to reside immediately downstream from skp, a gene previously identified as the firA locus...
  9. Jenkins R, Heslip K, Meagher J, Stuckey J, Dotson G. Structural basis for the recognition of peptide RJPXD33 by acyltransferases in lipid A biosynthesis. J Biol Chem. 2014;289:15527-35 pubmed publisher
    ..Overlay of the LpxA-RJPXD33 structure with E. coli LpxD identified a complementary peptide binding pocket within LpxD and serves as a model for further biochemical characterization of RJPXD33 binding to LpxD. ..

More Information


  1. Anderson M, Robertson A, Macher I, Raetz C. Biosynthesis of lipid A in Escherichia coli: identification of UDP-3-O-[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine as a precursor of UDP-N2,O3-bis[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine. Biochemistry. 1988;27:1908-17 pubmed
    ..Our observations provide strong evidence that UDP-3-O-[(R)-3-hydroxymyristoyl]glucosamine is a true intermediate in the biosynthesis of UDP-2,3-diacyl-GlcN and lipid A...
  2. Anderson M, Bulawa C, Raetz C. The biosynthesis of gram-negative endotoxin. Formation of lipid A precursors from UDP-GlcNAc in extracts of Escherichia coli. J Biol Chem. 1985;260:15536-41 pubmed
    ..No reaction is detected with fatty acyl-CoA or free fatty acid. The fatty acylation of sugar nucleotides has not been reported previously in E. coli or any other organism. ..
  3. Vuorio R, Harkonen T, Tolvanen M, Vaara M. The novel hexapeptide motif found in the acyltransferases LpxA and LpxD of lipid A biosynthesis is conserved in various bacteria. FEBS Lett. 1994;337:289-92 pubmed
    ..Accordingly, LpxA and LpxD of various bacterial origins can now be regarded as structurally very unusual enzymes, largely consisting of hexad repeats. ..
  4. Hirvas L, Coleman J, Koski P, Vaara M. Bacterial 'histone-like protein I' (HLP-I) is an outer membrane constituent?. FEBS Lett. 1990;262:123-6 pubmed
    ..coli which is close to the Ipx genes involved in the biosynthesis of lipopolysaccharides. The localization of the OmpH/HLP-I protein in the cell is discussed. ..
  5. Aasland R, Coleman J, Holck A, Smith C, Raetz C, Kleppe K. Identity of the 17-kilodalton protein, a DNA-binding protein from Escherichia coli, and the firA gene product. J Bacteriol. 1988;170:5916-8 pubmed
    ..encoded by the skp gene of Escherichia coli, was found to be identical to histonelike protein I, the product of the firA gene...
  6. Kelly T, Stachula S, Raetz C, Anderson M. The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis. J Biol Chem. 1993;268:19866-74 pubmed
    The possibility that the firA gene of Escherichia coli (Dicker, I. B., and Seetharam, S. (1991) Mol. Microbiol...
  7. Helander I, Lindner B, Seydel U, Vaara M. Defective biosynthesis of the lipid A component of temperature-sensitive firA (omsA) mutant of Escherichia coli. Eur J Biochem. 1993;212:363-9 pubmed
    The biosynthesis of lipid A component was shown to be defective in a temperature-sensitive firA mutant of Escherichia coli...
  8. Schnaitman C, Klena J. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol Rev. 1993;57:655-82 pubmed
  9. Bartling C, Raetz C. Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis. Biochemistry. 2008;47:5290-302 pubmed publisher
    ..Mutagenesis implicates E. coli H239 but excludes H276 as the catalytic base, and neither residue is likely to stabilize the oxyanion intermediate. ..
  10. Bartling C, Raetz C. Crystal structure and acyl chain selectivity of Escherichia coli LpxD, the N-acyltransferase of lipid A biosynthesis. Biochemistry. 2009;48:8672-83 pubmed publisher
  11. Vuorio R, Vaara M. Comparison of the phenotypes of the lpxA and lpxD mutants of Escherichia coli. FEMS Microbiol Lett. 1995;134:227-32 pubmed
    ..A method for the estimation of lipid A synthesis rate was developed. ..
  12. Coleman J, Raetz C. First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene. J Bacteriol. 1988;170:1268-74 pubmed
  13. Hirvas L, Koski P, Vaara M. The ompH gene of Yersinia enterocolitica: cloning, sequencing, expression, and comparison with known enterobacterial ompH sequences. J Bacteriol. 1991;173:1223-9 pubmed
    ..coli and S. typhimurium in the Enterobacteriaceae family, these results might indicate that most or all strains of the family Enterobacteriaceae have OmpH proteins remarkably homologous to those now sequenced. ..
  14. Roy A, Coleman J. Mutations in firA, encoding the second acyltransferase in lipopolysaccharide biosynthesis, affect multiple steps in lipopolysaccharide biosynthesis. J Bacteriol. 1994;176:1639-46 pubmed
    The product of the firA (ssc) gene is essential for growth and for the integrity of the outer membrane of Escherichia coli and Salmonella typhimurium. Recently, Kelly and coworkers (T. M. Kelly, S. A. Stachula, C. R. H. Raetz, and M. S...
  15. Newmister S, Sherman D. Crystal structures of acyl carrier protein in complex with two catalytic partners show a dynamic role in cellular metabolism. Chembiochem. 2014;15:1079-81 pubmed publisher
    ..These studies highlight key interactions of transient ACP-partner complexes and define the dynamic movements of ACP that facilitate substrate delivery and trigger complex dissociation. ..
  16. Tsuruoka T, Ito M, Tomioka S, Hirata A, Matsuhashi M. Thermosensitive omsA mutation of Escherichia coli that causes thermoregulated release of periplasmic proteins. J Bacteriol. 1988;170:5229-35 pubmed
    A mutant of Escherichia coli with a thermosensitive defect, possibly in the outer membrane (omsA mutant), was isolated from E. coli K-12 by mutagenization and selection for thermosensitivity and beta-lactam supersensitivity of growth...
  17. Vuorio R, Vaara M. Mutants carrying conditionally lethal mutations in outer membrane genes omsA and firA (ssc) are phenotypically similar, and omsA is allelic to firA. J Bacteriol. 1992;174:7090-7 pubmed
    ..mutant SS-C of Salmonella typhimurium and shown that this gene is analogous to the Escherichia coli gene firA (L. Hirvas, P. Koski, and M. Vaara, EMBO J. 10:1017-1023, 1991)...
  18. Dicker I, Seetharam S. What is known about the structure and function of the Escherichia coli protein FirA?. Mol Microbiol. 1992;6:817-23 pubmed
    The firA gene is essential for growth of Escherichia coli growth and lies in the 4-minute region of the genome. firA encodes the FirA protein which contains 341 amino acids and has an apparent molecular mass of 36 kDa...