Gene Symbol: ompX
Description: outer membrane protein X
Alias: ECK0803, JW0799, ybiG
Species: Escherichia coli str. K-12 substr. MG1655
Products:     ompX

Top Publications

  1. Stepanova E, Wang M, Severinov K, Borukhov S. Early transcriptional arrest at Escherichia coli rplN and ompX promoters. J Biol Chem. 2009;284:35702-13 pubmed publisher
    ..We propose that promoter-proximal arrest is a common feature of many bacterial promoters and may represent an important physiological target of regulation by transcript cleavage factors. ..
  2. De Lay N, Gottesman S. The Crp-activated small noncoding regulatory RNA CyaR (RyeE) links nutritional status to group behavior. J Bacteriol. 2009;191:461-76 pubmed publisher using microarray analysis and Northern blotting that several genes are negatively regulated by CyaR, including ompX, encoding a major outer membrane protein; luxS, encoding the autoinducer-2 synthase; nadE, encoding an essential ..
  3. Fernandez C, Adeishvili K, Wuthrich K. Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles. Proc Natl Acad Sci U S A. 2001;98:2358-63 pubmed
    The (2)H,(13)C,(15)N-labeled, 148-residue integral membrane protein OmpX from Escherichia coli was reconstituted with dihexanoyl phosphatidylcholine (DHPC) in mixed micelles of molecular mass of about 60 kDa...
  4. Mahalakshmi R, Franzin C, Choi J, Marassi F. NMR structural studies of the bacterial outer membrane protein OmpX in oriented lipid bilayer membranes. Biochim Biophys Acta. 2007;1768:3216-24 pubmed
    ..Here we present solid-state NMR spectra of the bacterial outer membrane protein OmpX in oriented lipid bilayer membranes...
  5. Fernandez C, Hilty C, Bonjour S, Adeishvili K, Pervushin K, Wuthrich K. Solution NMR studies of the integral membrane proteins OmpX and OmpA from Escherichia coli. FEBS Lett. 2001;504:173-8 pubmed
    ..proteins are discussed and illustrated with results obtained with the Escherichia coli integral membrane proteins OmpX and OmpA in mixed micelles with the detergent dihexanoylphosphatidylcholine (DHPC)...
  6. Hilty C, Wider G, Fernández C, Wuthrich K. Stereospecific assignments of the isopropyl methyl groups of the membrane protein OmpX in DHPC micelles. J Biomol NMR. 2003;27:377-82 pubmed
    ..For example, 3D 15N- and 13C-resolved [1H,1H]-NOESY spectra of the E. Coli membrane protein OmpX in mixed micelles with DHPC, which have an overall molecular weight of about 60 kDa, showed that about 50% of all ..
  7. Mecsas J, Welch R, Erickson J, Gross C. Identification and characterization of an outer membrane protein, OmpX, in Escherichia coli that is homologous to a family of outer membrane proteins including Ail of Yersinia enterocolitica. J Bacteriol. 1995;177:799-804 pubmed
    ..Sequence analysis of this gene indicated that it encodes an outer membrane protein which is 83% identical to OmpX in Enterobacter cloacae, leading us to name this gene ompX...
  8. Wu L, Lin X, Peng X. From proteome to genome for functional characterization of pH-dependent outer membrane proteins in Escherichia coli. J Proteome Res. 2009;8:1059-70 pubmed publisher
    ..Among the differentially expressed proteins identified, OstA, TolC, OmpT, OmpP OmpC, Trak, OmpX, Dps, LamB, Tsx, FadL, OmpW, and OmpF were characterized as pH-related OM proteins...
  9. Fernández C, Hilty C, Wider G, Güntert P, Wuthrich K. NMR structure of the integral membrane protein OmpX. J Mol Biol. 2004;336:1211-21 pubmed
    The structure of the integral membrane protein OmpX from Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints...

More Information


  1. Lomovskaya O, Kidwell J, Matin A. Characterization of the sigma 38-dependent expression of a core Escherichia coli starvation gene, pexB. J Bacteriol. 1994;176:3928-35 pubmed
    ..The -10 and -35 regions of pexB resembled those of some but not all genes known to be controlled by sigma 38. ..
  2. Molloy M, Herbert B, Walsh B, Tyler M, Traini M, Sanchez J, et al. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis. 1998;19:837-44 pubmed publisher
    ..Two of these outer membrane proteins (Omp), OmpW and OmpX, have previously been known only as an open reading frame in E...
  3. Pautsch A, Vogt J, Model K, Siebold C, Schulz G. Strategy for membrane protein crystallization exemplified with OmpA and OmpX. Proteins. 1999;34:167-72 pubmed
    The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses...
  4. Idicula Thomas S, Balaji P. Correlation between the structural stability and aggregation propensity of proteins. In Silico Biol. 2007;7:225-37 pubmed
  5. Tafer H, Hiller S, Hilty C, Fernández C, Wuthrich K. Nonrandom structure in the urea-unfolded Escherichia coli outer membrane protein X (OmpX). Biochemistry. 2004;43:860-9 pubmed
    ..nuclear magnetic resonance (NMR) spectroscopy, the urea-unfolded form of the outer membrane protein X (OmpX) from Escherichia coli has been structurally characterized...
  6. Koebnik R, Locher K, Van Gelder P. Structure and function of bacterial outer membrane proteins: barrels in a nutshell. Mol Microbiol. 2000;37:239-53 pubmed
    ..They include the OmpA membrane domain, the OmpX protein, phospholipase A, general porins (OmpF, PhoE), substrate-specific porins (LamB, ScrY) and the TonB-..
  7. Maisnier Patin K, Malissard M, Jeannin P, Haeuw J, Corbière J, Hoeffel G, et al. The outer membrane protein X from Escherichia coli exhibits immune properties. Vaccine. 2003;21:3765-74 pubmed
    ..In this study, we investigated whether OmpX, another member of the OMP family and structurally related to OmpA, exhibits the same immune properties...
  8. Otto K, Hermansson M. Inactivation of ompX causes increased interactions of type 1 fimbriated Escherichia coli with abiotic surfaces. J Bacteriol. 2004;186:226-34 pubmed
    ..coli strain included reductions in the levels of several outer membrane proteins, one of which was identified as OmpX. Here, the phenotypes of mutant strains that differ at the ompX locus were studied with regard to adhesion, cell ..
  9. Nakashima K, Horikoshi K, Mizuno T. Effect of hydrostatic pressure on the synthesis of outer membrane proteins in Escherichia coli. Biosci Biotechnol Biochem. 1995;59:130-2 pubmed
    ..It was also found that the expression of a presumed novel outer membrane protein, named OmpX, was affected by both the medium osmolarity and pressure in a manner independent of the function of EnvZ and OmpR.
  10. Johansen J, Eriksen M, Kallipolitis B, Valentin Hansen P. Down-regulation of outer membrane proteins by noncoding RNAs: unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX regulatory case. J Mol Biol. 2008;383:1-9 pubmed publisher
    ..Here we report the identification of the sRNA (CyaR, cyclic AMP-activated RNA) that promotes decay of the ompX mRNA...
  11. Mecsas J, Rouviere P, Erickson J, Donohue T, Gross C. The activity of sigma E, an Escherichia coli heat-inducible sigma-factor, is modulated by expression of outer membrane proteins. Genes Dev. 1993;7:2618-28 pubmed
  12. Lin X, Wu L, Li H, Wang S, Peng X. Downregulation of Tsx and OmpW and upregulation of OmpX are required for iron homeostasis in Escherichia coli. J Proteome Res. 2008;7:1235-43 pubmed publisher
    ..Notably, three novel proteins, Tsx, OmpW, and OmpX, related to iron homeostasis were identified; Tsx and OmpW were downregulated, and OmpX was upregulated...
  13. Zhang Q, Horst R, Geralt M, Ma X, Hong W, Finn M, et al. Microscale NMR screening of new detergents for membrane protein structural biology. J Am Chem Soc. 2008;130:7357-63 pubmed publisher
    ..coli outer membrane protein OmpX as a test case...
  14. Burgess N, Dao T, Stanley A, Fleming K. Beta-barrel proteins that reside in the Escherichia coli outer membrane in vivo demonstrate varied folding behavior in vitro. J Biol Chem. 2008;283:26748-58 pubmed publisher
    ..We cloned, expressed, and folded nine OMPs: outer membrane protein X (OmpX), OmpW, OmpA, the crcA gene product (PagP), OmpT, outer membrane phospholipase A (OmpLa), the fadl gene product (..
  15. Lee D, Hilty C, Wider G, Wuthrich K. Effective rotational correlation times of proteins from NMR relaxation interference. J Magn Reson. 2006;178:72-6 pubmed
    ..aldolase from Staphylococcus aureus, the uniformly 15N-labeled Escherichia coli outer membrane protein X (OmpX) in 60 kDa mixed OmpX/DHPC micelles with approximately 90 molecules of unlabeled 1,2-dihexanoyl-sn-glycero-3-..
  16. Rice J, Schohn A, Bessette P, Boulware K, Daugherty P. Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands. Protein Sci. 2006;15:825-36 pubmed
    ..libraries, a new scaffold was developed through circular permutation of the Escherichia coli outer membrane protein OmpX that presents both N and C termini on the external cell surface...
  17. Mahalakshmi R, Marassi F. Orientation of the Escherichia coli outer membrane protein OmpX in phospholipid bilayer membranes determined by solid-State NMR. Biochemistry. 2008;47:6531-8 pubmed publisher achieved by incorporating a few orientation restraints, measured for the Escherichia coli outer membrane protein OmpX in magnetically oriented lipid bilayers (bicelles), in a simulated annealing calculation with the coordinates of ..
  18. Fernández C, Wuthrich K. NMR solution structure determination of membrane proteins reconstituted in detergent micelles. FEBS Lett. 2003;555:144-50 pubmed
    ..For the Escherichia coli outer membrane protein X (OmpX) in dihexanoylphosphatidylcholine (DHPC) micelles, such studies showed that the central part of the protein is ..
  19. Kräutler V, Hiller S, Hünenberger P. Residual structure in a peptide fragment of the outer membrane protein X under denaturing conditions: a molecular dynamics study. Eur Biophys J. 2010;39:1421-32 pubmed publisher
    The Escherichia coli outer membrane protein X (OmpX) contains two polypeptide segments that present nonrandom residual structure in 8 M aqueous urea, whereas the remainder of the protein is in a flexibly disordered conformation (Tafer et ..
  20. Vogt J, Schulz G. The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence. Structure. 1999;7:1301-9 pubmed
    The integral outer membrane protein X (OmpX) from Escherichia coli belongs to a family of highly conserved bacterial proteins that promote bacterial adhesion to and entry into mammalian cells...
  21. Burmann B, Wang C, Hiller S. Conformation and dynamics of the periplasmic membrane-protein-chaperone complexes OmpX-Skp and tOmpA-Skp. Nat Struct Mol Biol. 2013;20:1265-72 pubmed publisher
    ..The dynamic state allows for energy-independent substrate release and provides a general paradigm for the conformation of OMP polypeptides bound to energy-independent chaperones...
  22. Otto K, Norbeck J, Larsson T, Karlsson K, Hermansson M. Adhesion of type 1-fimbriated Escherichia coli to abiotic surfaces leads to altered composition of outer membrane proteins. J Bacteriol. 2001;183:2445-53 pubmed
    ..of flight mass spectrometry, six of the proteins resolved by 2D analysis were identified as BtuB, EF-Tu, OmpA, OmpX, Slp, and TolC...
  23. Sigdel T, Cilliers R, Gursahaney P, Thompson P, Easton J, Crowder M. Probing the adaptive response of Escherichia coli to extracellular Zn(II). Biometals. 2006;19:461-71 pubmed
    ..coli proteins, and the results are discussed in light of recent genomic profiling studies on the adaptive response of E. coli cells to stress by Zn(II) excess. ..
  24. Hilty C, Wider G, Fernández C, Wuthrich K. Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy with the use of paramagnetic reagents. Chembiochem. 2004;5:467-73 pubmed
    ..detergent dihexanoylphosphatidylcholine (DHPC) and one molecule of the Escherichia coli outer-membrane protein X (OmpX), which had previously been extensively studied by solution NMR techniques...
  25. Jacoboni I, Martelli P, Fariselli P, De Pinto V, Casadio R. Prediction of the transmembrane regions of beta-barrel membrane proteins with a neural network-based predictor. Protein Sci. 2001;10:779-87 pubmed
    ..In addition, protein topology is assigned on the basis of the location of the longest loops in the models. We propose this as a general method to fill the gap of the prediction of beta-barrel membrane proteins. ..
  26. Wimley W. The versatile beta-barrel membrane protein. Curr Opin Struct Biol. 2003;13:404-11 pubmed
    ..New research is revealing the variety of beta-barrel structures and the variety of functions performed by these versatile proteins. ..