ompW

Summary

Gene Symbol: ompW
Description: outer membrane protein W
Alias: ECK1250, JW1248, yciD
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Hong H, Patel D, Tamm L, van den Berg B. The outer membrane protein OmpW forms an eight-stranded beta-barrel with a hydrophobic channel. J Biol Chem. 2006;281:7568-77 pubmed
    Escherichia coli OmpW belongs to a family of small outer membrane proteins that are widespread in Gram-negative bacteria...
  2. Molloy M, Herbert B, Slade M, Rabilloud T, Nouwens A, Williams K, et al. Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem. 2000;267:2871-81 pubmed
    ..In an additional demonstration we show the value of parallel protein analysis to document changes in E. coli OMP expression as influenced by culture temperature...
  3. Han M, Lee S, Hong S. Comparative analysis of envelope proteomes in Escherichia coli B and K-12 strains. J Microbiol Biotechnol. 2012;22:470-8 pubmed
    ..Thus, these findings suggest that E. coli K-12 and its derivatives will be more favorable strains in certain biotechnological applications, such as cell surface display or membrane engineering studies. ..
  4. Stoltzfus A, Leslie J, Milkman R. Molecular evolution of the Escherichia coli chromosome. I. Analysis of structure and natural variation in a previously uncharacterized region between trp and tonB. Genetics. 1988;120:345-58 pubmed
    ..The effects of genetic recombination between strains, recently found to be significant on a large scale in E. coli, are also apparent in the region between trp and tonB. ..
  5. Lin X, Li H, Wang C, Peng X. Proteomic analysis of nalidixic acid resistance in Escherichia coli: identification and functional characterization of OM proteins. J Proteome Res. 2008;7:2399-405 pubmed publisher
    ..The OM proteins TolC, OmpT, OmpC and OmpW were found to be up-regulated, and FadL was down-regulated in the NA-resistant E. coli strains...
  6. Li W, Wen L, Li C, Chen R, Ye Z, Zhao J, et al. Contribution of the outer membrane protein OmpW in Escherichia coli to complement resistance from binding to factor H. Microb Pathog. 2016;98:57-62 pubmed publisher
    ..We have previously demonstrated that OmpW, also an 8-stranded ?-barrel protein that was identified a decade ago, protects bacteria against host phagocytosis...
  7. Wu X, Tian L, Zou H, Wang C, Yu Z, Tang C, et al. Outer membrane protein OmpW of Escherichia coli is required for resistance to phagocytosis. Res Microbiol. 2013;164:848-55 pubmed publisher
    ..This resistance function of OmpW, which was recently identified as an eight-stranded ?-barrel protein, was investigated in this study...
  8. Lee J, Sung B, Yu B, Lee J, Lee S, Kim M, et al. Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli. Nucleic Acids Res. 2008;36:e102 pubmed publisher
    ..These results and the general applicability of this platform clearly indicate that novel ATFs are powerful tools for the phenotypic engineering of microorganisms and can facilitate microbial functional genomic studies. ..
  9. Zhang D, Jiang B, Xiang Z, Wang S. Functional characterisation of altered outer membrane proteins for tetracycline resistance in Escherichia coli. Int J Antimicrob Agents. 2008;32:315-9 pubmed publisher
    ..In this study, upregulation of FimD, Tsx, OmpW, OmpC and TolC and downregulation of LamB in response to tetracycline were detected and confirmed by two-..

More Information

Publications20

  1. Huang C, Lin X, Wu L, Zhang D, Liu D, Wang S, et al. Systematic identification of the subproteome of Escherichia coli cell envelope reveals the interaction network of membrane proteins and membrane-associated peripheral proteins. J Proteome Res. 2006;5:3268-76 pubmed
    ..a number of previously unrecognized membrane-interacting protein complexes, such as the complex consisting of OmpW and fumarate reductase. This established complete proteomic profile of E...
  2. Xu C, Lin X, Ren H, Zhang Y, Wang S, Peng X. Analysis of outer membrane proteome of Escherichia coli related to resistance to ampicillin and tetracycline. Proteomics. 2006;6:462-73 pubmed
    ..were known as antibiotic-resistant proteins, including TolC, OmpC and YhiU, while FimD precursor, LamB, Tsx, YfiO, OmpW, NlpB were first reported here to be antibiotic-resistance-related proteins...
  3. Pilsl H, Smajs D, Braun V. Characterization of colicin S4 and its receptor, OmpW, a minor protein of the Escherichia coli outer membrane. J Bacteriol. 1999;181:3578-81 pubmed
    ..By contrast, the predicted receptor binding domain shows no sequence similarities to other colicins. Mutants that lacked the OmpW protein were resistant to colicin S4.
  4. Lin X, Wu L, Li H, Wang S, Peng X. Downregulation of Tsx and OmpW and upregulation of OmpX are required for iron homeostasis in Escherichia coli. J Proteome Res. 2008;7:1235-43 pubmed publisher
    ..Notably, three novel proteins, Tsx, OmpW, and OmpX, related to iron homeostasis were identified; Tsx and OmpW were downregulated, and OmpX was upregulated...
  5. Albrecht R, Zeth K, Söding J, Lupas A, Linke D. Expression, crystallization and preliminary X-ray crystallographic studies of the outer membrane protein OmpW from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:415-8 pubmed
    b>OmpW is an eight-stranded 21 kDa molecular-weight beta-barrel protein from the outer membrane of Gram-negative bacteria...
  6. Burgess N, Dao T, Stanley A, Fleming K. Beta-barrel proteins that reside in the Escherichia coli outer membrane in vivo demonstrate varied folding behavior in vitro. J Biol Chem. 2008;283:26748-58 pubmed publisher
    ..We cloned, expressed, and folded nine OMPs: outer membrane protein X (OmpX), OmpW, OmpA, the crcA gene product (PagP), OmpT, outer membrane phospholipase A (OmpLa), the fadl gene product (FadL), ..
  7. Li H, Wang B, Xu W, Lin X, Peng X. Identification and network of outer membrane proteins regulating streptomysin resistance in Escherichia coli. J Proteome Res. 2008;7:4040-9 pubmed publisher
    ..TolC, OmpT and LamB were found to be up-regulated, and FadL, OmpW and a location-unknown protein Dps were down-regulated in the SM-resistant E. coli strain...
  8. Wu L, Lin X, Peng X. From proteome to genome for functional characterization of pH-dependent outer membrane proteins in Escherichia coli. J Proteome Res. 2009;8:1059-70 pubmed publisher
    ..the differentially expressed proteins identified, OstA, TolC, OmpT, OmpP OmpC, Trak, OmpX, Dps, LamB, Tsx, FadL, OmpW, and OmpF were characterized as pH-related OM proteins...
  9. Brambilla L, Morán Barrio J, Viale A. Expression of the Escherichia coli ompW colicin S4 receptor gene is regulated by temperature and modulated by the H-NS and StpA nucleoid-associated proteins. FEMS Microbiol Lett. 2014;352:238-44 pubmed publisher
    The OmpW family consists of a ubiquitous group of small outer membrane (OM) ?-barrel proteins of Gram-negative bacteria with proposed roles in environmental adaptation but poorly understood mechanisms of expression...
  10. Johansen J, Rasmussen A, Overgaard M, Valentin Hansen P. Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins. J Mol Biol. 2006;364:1-8 pubmed
    ..Transient induction of RybB resulted in decreased levels of the mRNAs encoding OmpC and OmpW. sigmaE -mediated regulation of ompC and ompW expression was abolished in strains lacking RybB or Hfq...
  11. Molloy M, Herbert B, Walsh B, Tyler M, Traini M, Sanchez J, et al. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis. 1998;19:837-44 pubmed publisher
    ..Two of these outer membrane proteins (Omp), OmpW and OmpX, have previously been known only as an open reading frame in E...