ompR

Summary

Gene Symbol: ompR
Description: response regulator in two-component regulatory system with EnvZ
Alias: ECK3392, JW3368, kmt, ompB
Species: Escherichia coli str. K-12 substr. MG1655
Products:     ompR

Top Publications

  1. Reimann S, Wolfe A. A critical process controlled by MalT and OmpR is revealed through synthetic lethality. J Bacteriol. 2009;191:5320-4 pubmed publisher
    ..Here we report that cells lacking OmpR and harboring constitutively active MalT undergo premature death that involves increased expression of the outer ..
  2. Kanamaru K, Mizuno T. Signal transduction and osmoregulation in Escherichia coli: a novel mutant of the positive regulator, OmpR, that functions in a phosphorylation-independent manner. J Biochem. 1992;111:425-30 pubmed
    ..coli, expression of the outer membrane proteins, OmpF and OmpC, is regulated by the regulatory factors, EnvZ and OmpR, at the transcriptional level in response to the medium osmolarity...
  3. Rhee J, Sheng W, Morgan L, Nolet R, Liao X, Kenney L. Amino acids important for DNA recognition by the response regulator OmpR. J Biol Chem. 2008;283:8664-77 pubmed publisher
    ..b>OmpR is a winged helix-turnhelix DNA-binding protein that functions as a global regulator in bacteria and is also ..
  4. Groban E, Clarke E, Salis H, Miller S, Voigt C. Kinetic buffering of cross talk between bacterial two-component sensors. J Mol Biol. 2009;390:380-93 pubmed publisher
    ..In this work, we studied the EnvZ/OmpR and CpxA/CpxR systems from Escherichia coli, which have been shown previously to exhibit slow cross talk in vitro...
  5. Siryaporn A, Perchuk B, Laub M, Goulian M. Evolving a robust signal transduction pathway from weak cross-talk. Mol Syst Biol. 2010;6:452 pubmed publisher
    ..by combining a small number of mutations in CpxA that individually increase phosphorylation of the non-partner RR OmpR, phosphatase activity against phospho-OmpR emerges...
  6. Mizuno T, Mizushima S. Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the porin genes. Mol Microbiol. 1990;4:1077-82 pubmed
    ..EnvZ and OmpR, which are positive regulatory factors for the transcriptional osmotic regulation of the ompF and ompC genes, ..
  7. Prigent Combaret C, Brombacher E, Vidal O, Ambert A, Lejeune P, Landini P, et al. Complex regulatory network controls initial adhesion and biofilm formation in Escherichia coli via regulation of the csgD gene. J Bacteriol. 2001;183:7213-23 pubmed
    The Escherichia coli OmpR/EnvZ two-component regulatory system, which senses environmental osmolarity, also regulates biofilm formation. Up mutations in the ompR gene, such as the ompR234 mutation, stimulate laboratory strains of E...
  8. Forst S, Roberts D. Signal transduction by the EnvZ-OmpR phosphotransfer system in bacteria. Res Microbiol. 1994;145:363-73 pubmed
  9. Lan C, Igo M. Differential expression of the OmpF and OmpC porin proteins in Escherichia coli K-12 depends upon the level of active OmpR. J Bacteriol. 1998;180:171-4 pubmed
    We have generated a mutant form of the OmpR regulatory protein, OmpRD55E, that is active independent of the EnvZ kinase...

More Information

Publications81

  1. Batchelor E, Goulian M. Imaging OmpR localization in Escherichia coli. Mol Microbiol. 2006;59:1767-78 pubmed
    We have used a fusion of GFP to the response regulator OmpR to image the spatial distribution of OmpR in live cells of Escherichia coli...
  2. Yoshida T, Cai S, Inouye M. Interaction of EnvZ, a sensory histidine kinase, with phosphorylated OmpR, the cognate response regulator. Mol Microbiol. 2002;46:1283-94 pubmed
    EnvZ is a sensory histidine kinase in Escherichia coli to regulate the phosphorylation of OmpR, its cognate response regulator, required for the expression of genes for outer membrane porin proteins...
  3. Goh E, Siino D, Igo M. The Escherichia coli tppB (ydgR) gene represents a new class of OmpR-regulated genes. J Bacteriol. 2004;186:4019-24 pubmed
    The EnvZ/OmpR two-component regulatory system plays a critical role in the Escherichia coli stress response. In this study, we examined the expression of a new OmpR-regulated gene, ydgR...
  4. Tsui P, Huang L, Freundlich M. Integration host factor binds specifically to multiple sites in the ompB promoter of Escherichia coli and inhibits transcription. J Bacteriol. 1991;173:5800-7 pubmed
    ..In the present study, we showed that IHF is a direct negative effector of the ompB operon of E. coli...
  5. Siryaporn A, Goulian M. Cross-talk suppression between the CpxA-CpxR and EnvZ-OmpR two-component systems in E. coli. Mol Microbiol. 2008;70:494-506 pubmed publisher
    ..such interference, we have examined cross-talk between the histidine kinase CpxA and non-cognate response regulator OmpR in Escherichia coli...
  6. Mart nez Hackert E, Stock A. The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor. Structure. 1997;5:109-24 pubmed
    ..the two component family of signal transduction proteins: the histidine kinase, EnvZ, and the response regulator, OmpR. OmpR belongs to a subfamily of at least 50 response regulators with homologous C-terminal DNA-binding domains of ..
  7. Shinar G, Milo R, Martinez M, Alon U. Input output robustness in simple bacterial signaling systems. Proc Natl Acad Sci U S A. 2007;104:19931-5 pubmed
    ..It explains how specific molecular details can work together to provide robustness. Moreover, it suggests an approach that can help identify a wide family of nonequilibrium mechanisms that potentially have robust input-output relations. ..
  8. Cai S, Inouye M. EnvZ-OmpR interaction and osmoregulation in Escherichia coli. J Biol Chem. 2002;277:24155-61 pubmed
    ..Although both ompR and envZ genes are located on the ompB locus under the control of the ompB promoter and transcribed as a single polycistronic mRNA, the expression of envZ ..
  9. Igo M, Ninfa A, Stock J, Silhavy T. Phosphorylation and dephosphorylation of a bacterial transcriptional activator by a transmembrane receptor. Genes Dev. 1989;3:1725-34 pubmed
    ..the bacterial Omp, Che, and Ntr systems involves the phosphorylation and dephosphorylation of response regulators (OmpR, CheY and CheB, NRI) that share a homologous domain...
  10. Liljestrom P, Laamanen I, Palva E. Structure and expression of the ompB operon, the regulatory locus for the outer membrane porin regulon in Salmonella typhimurium LT-2. J Mol Biol. 1988;201:663-73 pubmed
    The ompB operon of Salmonella typhimurium encodes a positive transcriptional regulator OmpR and an inner membrane protein EnvZ. Both proteins are needed for the proper expression of the outer membrane proteins OmpC and OmpF...
  11. Huang K, Igo M. Identification of the bases in the ompF regulatory region, which interact with the transcription factor OmpR. J Mol Biol. 1996;262:615-28 pubmed
    ..A key element of this regulation is the interaction between the transcriptional factor OmpR and the cis-acting regulatory region of ompF...
  12. Jubelin G, Vianney A, Beloin C, Ghigo J, Lazzaroni J, Lejeune P, et al. CpxR/OmpR interplay regulates curli gene expression in response to osmolarity in Escherichia coli. J Bacteriol. 2005;187:2038-49 pubmed
    ..b>OmpR was shown to activate csgD expression, resulting in curli production...
  13. Vidal O, Longin R, Prigent Combaret C, Dorel C, Hooreman M, Lejeune P. Isolation of an Escherichia coli K-12 mutant strain able to form biofilms on inert surfaces: involvement of a new ompR allele that increases curli expression. J Bacteriol. 1998;180:2442-9 pubmed
    ..mutation, resulting in the replacement of a leucine by an arginine residue at position 43 in the regulatory protein OmpR, was responsible for this phenotype...
  14. Khorchid A, Inouye M, Ikura M. Structural characterization of Escherichia coli sensor histidine kinase EnvZ: the periplasmic C-terminal core domain is critical for homodimerization. Biochem J. 2005;385:255-64 pubmed
  15. Shin S, Park C. Modulation of flagellar expression in Escherichia coli by acetyl phosphate and the osmoregulator OmpR. J Bacteriol. 1995;177:4696-702 pubmed
    ..Furthermore, the following evidence indicates that the phosphorylation of OmpR, a trans factor for osmoregulation, regulates flagellar expression...
  16. Kenney L, Bauer M, Silhavy T. Phosphorylation-dependent conformational changes in OmpR, an osmoregulatory DNA-binding protein of Escherichia coli. Proc Natl Acad Sci U S A. 1995;92:8866-70 pubmed
    ..Osmoregulated porin gene expression in Escherichia coli is controlled by the two-component regulatory system EnvZ and OmpR. EnvZ, the osmosensor, is an inner membrane protein and a histidine kinase...
  17. Waukau J, Forst S. Molecular analysis of the signaling pathway between EnvZ and OmpR in Escherichia coli. J Bacteriol. 1992;174:1522-7 pubmed
    b>OmpR is a DNA-binding protein that regulates transcription of ompF and ompC. The activity of OmpR is controlled by the inner membrane osmosensor, EnvZ...
  18. Hall M, Silhavy T. The ompB locus and the regulation of the major outer membrane porin proteins of Escherichia coli K12. J Mol Biol. 1981;146:23-43 pubmed
  19. Thompson K, Rhodius V, Gottesman S. SigmaE regulates and is regulated by a small RNA in Escherichia coli. J Bacteriol. 2007;189:4243-56 pubmed
    ..One contained the gene for the response regulator OmpR; the second contained mipA, encoding a murein hydrolase...
  20. Csonka L, Hanson A. Prokaryotic osmoregulation: genetics and physiology. Annu Rev Microbiol. 1991;45:569-606 pubmed
  21. Mattison K, Oropeza R, Byers N, Kenney L. A phosphorylation site mutant of OmpR reveals different binding conformations at ompF and ompC. J Mol Biol. 2002;315:497-511 pubmed
    ..porins in response to environmental osmolarity consists of the sensor kinase EnvZ and the response regulator OmpR. Phosphorylated OmpR activates expression of the OmpF porin at low osmolarity, and at high osmolarity represses ..
  22. Yoshida T, Qin L, Egger L, Inouye M. Transcription regulation of ompF and ompC by a single transcription factor, OmpR. J Biol Chem. 2006;281:17114-23 pubmed
    ..ompF and ompC genes of Escherichia coli are reciprocally regulated by a single transcription factor, phosphorylated OmpR (OmpR-P), depending upon medium osmolarity...
  23. Nanchen A, Schicker A, Revelles O, Sauer U. Cyclic AMP-dependent catabolite repression is the dominant control mechanism of metabolic fluxes under glucose limitation in Escherichia coli. J Bacteriol. 2008;190:2323-30 pubmed publisher
    ..the relevance of global transcriptional regulation by ArcA, ArcB, Cra, CreB, CreC, Crp, Cya, Fnr, Hns, Mlc, OmpR, and UspA on aerobic glucose catabolism in glucose-limited chemostat cultures at a growth rate of 0.1 h(-1)...
  24. Webb E, Downs D. Characterization of thiL, encoding thiamin-monophosphate kinase, in Salmonella typhimurium. J Biol Chem. 1997;272:15702-7 pubmed
    ..In contrast to other thi loci, thiL is not transcriptionally regulated by thiamin pyrophosphate. This result is consistent with a dual role for ThiL in de novo biosynthesis and in salvage of exogenous thiamin. ..
  25. Prigent Combaret C, Vidal O, Dorel C, Lejeune P. Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli. J Bacteriol. 1999;181:5993-6002 pubmed
    ..In this work, we show that bacteria within biofilms encounter higher-osmolarity conditions, greater oxygen limitation, and higher cell density than in the liquid phase. ..
  26. Taylor R, Hall M, Enquist L, Silhavy T. Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12. J Bacteriol. 1981;147:255-8 pubmed
    We report here on the cloning of a gene located within the ompB locus of E. coli K-12. This gene, designated ompR, resides on a 10.9-kilobase EcoRI fragment that we cloned, using a lambda vector and in vitro packaging techniques...
  27. McLaughlin W, Berman H. Statistical models for discerning protein structures containing the DNA-binding helix-turn-helix motif. J Mol Biol. 2003;330:43-55 pubmed
  28. Yoshida T, Qin L, Inouye M. Formation of the stoichiometric complex of EnvZ, a histidine kinase, with its response regulator, OmpR. Mol Microbiol. 2002;46:1273-82 pubmed
    EnvZ, a histidine kinase, and its cognate response regulator OmpR of Escherichia coli are responsible for adaptation to external osmotic changes by regulating the levels of the outer membrane porin proteins, OmpF and OmpC...
  29. Nara F, Matsuyama S, Mizuno T, Mizushima S. Molecular analysis of mutant ompR genes exhibiting different phenotypes as to osmoregulation of the ompF and ompC genes of Escherichia coli. Mol Gen Genet. 1986;202:194-9 pubmed
    ..The OmpR protein, a positive regulator of these genes, is involved in the osmoregulation (Dairi et al. 1985; Nara et al...
  30. Mizuno T, Wurtzel E, Inouye M. Osmoregulation of gene expression. II. DNA sequence of the envZ gene of the ompB operon of Escherichia coli and characterization of its gene product. J Biol Chem. 1982;257:13692-8 pubmed
  31. Coyer J, Andersen J, Forst S, Inouye M, Delihas N. micF RNA in ompB mutants of Escherichia coli: different pathways regulate micF RNA levels in response to osmolarity and temperature change. J Bacteriol. 1990;172:4143-50 pubmed
    ..For example, relative to the parental strain MC4100, the ompR101 mutant strain (which contains no functional OmpR) had about a 10-fold reduction in micF RNA, whereas the envZ11 strain showed about a 5-fold increase...
  32. Gerken H, Misra R. MzrA-EnvZ interactions in the periplasm influence the EnvZ/OmpR two-component regulon. J Bacteriol. 2010;192:6271-8 pubmed publisher
    MzrA was identified as a modulator of the EnvZ/OmpR two-component signal transduction system. Previous evidence indicated that MzrA interacts with EnvZ and modulates its enzymatic activities to influence OmpR phosphate (OmpR?P) levels...
  33. Wurtzel E, Chou M, Inouye M. Osmoregulation of gene expression. I. DNA sequence of the ompR gene of the ompB operon of Escherichia coli and characterization of its gene product. J Biol Chem. 1982;257:13685-91 pubmed
    The ompB region on the Escherichia coli chromosome codes for two genes, ompR and envZ, which are required for the osmolarity sensitive biosynthetic regulation of the outer membrane matrix proteins (porins), OmpF and ompC...
  34. Qin L, Dutta R, Kurokawa H, Ikura M, Inouye M. A monomeric histidine kinase derived from EnvZ, an Escherichia coli osmosensor. Mol Microbiol. 2000;36:24-32 pubmed
    ..We show that this protein, EnvZc[AAB], is soluble and exists as a stable monomer. The autophosphorylation and OmpR kinase activities of the monomeric EnvZc[AAB] are similar to that of the wild-type EnvZ, while OmpR-binding and ..
  35. Yamamoto K, Nagura R, Tanabe H, Fujita N, Ishihama A, Utsumi R. Negative regulation of the bolA1p of Escherichia coli K-12 by the transcription factor OmpR for osmolarity response genes. FEMS Microbiol Lett. 2000;186:257-62 pubmed
    To test whether OmpR is involved in regulation of the bolA1p, we investigated possible effects of ompR mutation on transcription from bolA1p. In vivo, bolA1p was found to be repressed by OmpR...
  36. Matsubara M, Mizuno T. EnvZ-independent phosphotransfer signaling pathway of the OmpR-mediated osmoregulatory expression of OmpC and OmpF in Escherichia coli. Biosci Biotechnol Biochem. 1999;63:408-14 pubmed
    The Escherichia coli EnvZ-OmpR regulatory system is a paradigm of intracellular signal transduction mediated by the well-documented phosphotransfer mechanism, by which the expression of the major outer membrane proteins, OmpC and OmpF, is ..
  37. Kobayashi H, Yoshida T, Inouye M. Significant enhanced expression and solubility of human proteins in Escherichia coli by fusion with protein S from Myxococcus xanthus. Appl Environ Microbiol. 2009;75:5356-62 pubmed publisher
  38. Prohinar P, Forst S, Reed D, Mandic Mulec I, Weiss J. OmpR-dependent and OmpR-independent responses of Escherichia coli to sublethal attack by the neutrophil bactericidal/permeability increasing protein. Mol Microbiol. 2002;43:1493-504 pubmed
    ..Initial effects include selective OmpR-dependent changes in the synthesis of outer membrane porins (OmpF and OmpC)...
  39. Lin X, Li H, Wang C, Peng X. Proteomic analysis of nalidixic acid resistance in Escherichia coli: identification and functional characterization of OM proteins. J Proteome Res. 2008;7:2399-405 pubmed publisher
    ..understanding of the mechanisms of NA resistance, we also characterized the role of the two-component system EnvZ/OmpR which is responsible for the regulation of OmpC and OmpF expression in response to NA resistance using their ..
  40. Igoshin O, Alves R, Savageau M. Hysteretic and graded responses in bacterial two-component signal transduction. Mol Microbiol. 2008;68:1196-215 pubmed publisher
    ..The phosphatase activity may also be modulated by environmental signals. Using the EnvZ/OmpR system as an example, we constructed mathematical models to examine the steady-state and kinetic properties of the ..
  41. Foo Y, Spahn C, Zhang H, Heilemann M, Kenney L. Single cell super-resolution imaging of E. coli OmpR during environmental stress. Integr Biol (Camb). 2015;7:1297-308 pubmed publisher
    ..The EnvZ/OmpR system in E...
  42. Ohashi K, Yamashino T, Mizuno T. Molecular basis for promoter selectivity of the transcriptional activator OmpR of Escherichia coli: isolation of mutants that can activate the non-cognate kdpABC promoter. J Biochem. 2005;137:51-9 pubmed
    Escherichia coli has two osmo-responsive two-component regulatory systems, the EnvZ-OmpR and KdpD-KdpE systems, each of which consists of a sensor histidine protein kinase and a response regulator...
  43. Mao W, Siegele D. Genetic analysis of the stationary phase-induced mcb operon promoter in Escherichia coli. Mol Microbiol. 1998;27:415-24 pubmed
    ..All but one of these mutations decreased OmpR-dependent activation of Pmcb transcription...
  44. Vianney A, Jubelin G, Renault S, Dorel C, Lejeune P, Lazzaroni J. Escherichia coli tol and rcs genes participate in the complex network affecting curli synthesis. Microbiology. 2005;151:2487-97 pubmed
    ..In the tol mutants, activation of csgDEFG occurred via OmpR and was dominant upon repression by RcsB and RcsA, while these two regulatory proteins repressed csgBA through a ..
  45. McBroom A, Johnson A, Vemulapalli S, Kuehn M. Outer membrane vesicle production by Escherichia coli is independent of membrane instability. J Bacteriol. 2006;188:5385-92 pubmed
    ..Detergent sensitivity, leakiness, and growth characteristics of the novel vesiculation mutant strains did not correlate with vesiculation levels, demonstrating that vesicle production is not predictive of envelope instability. ..
  46. Brissette R, Tsung K, Inouye M. Mutations in a central highly conserved non-DNA-binding region of OmpR, an Escherichia coli transcriptional activator, influence its DNA-binding ability. J Bacteriol. 1992;174:4907-12 pubmed
    b>OmpR is a transcriptional activator for the expression of outer membrane porin genes ompF and ompC in Escherichia coli. Its C-terminal half has been identified as the DNA-binding domain (K. Tsung, R. Brissette, and M. Inouye, J. Biol...
  47. Condemine G, Berrier C, Plumbridge J, Ghazi A. Function and expression of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli. J Bacteriol. 2005;187:1959-65 pubmed
    ..nanC expression is also activated by the regulators cyclic AMP-catabolite activator protein, OmpR, and CpxR...
  48. Okamura H, Hanaoka S, Nagadoi A, Makino K, Nishimura Y. Structural comparison of the PhoB and OmpR DNA-binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box. J Mol Biol. 2000;295:1225-36 pubmed
    ..The overall architecture is very close to that of the OmpR DNA-binding/transactivation domain, however, the conformation of the long turn region of PhoB, a putative ..
  49. Maeda S, Takayanagi K, Nishimura Y, Maruyama T, Sato K, Mizuno T. Activation of the osmoregulated ompC gene by the OmpR protein in Escherichia coli: a study involving synthetic OmpR-binding sequences. J Biochem. 1991;110:324-7 pubmed
    ..The OmpR and EnvZ proteins are transcriptional factors involved in this osmotic regulation of the ompC and ompF genes...
  50. Comeau D, Ikenaka K, Tsung K, Inouye M. Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins. J Bacteriol. 1985;164:578-84 pubmed
    The ompB operon of Escherichia coli contains the structural genes for two proteins, OmpR and EnvZ, which control the osmoregulated biosynthesis of the porin proteins OmpF and OmpC...
  51. Srividhya K, Krishnaswamy S. A simulation model of Escherichia coli osmoregulatory switch using E-CELL system. BMC Microbiol. 2004;4:44 pubmed
    ..well-studied two component regulatory systems comprising of the sensor, EnvZ and the cognate response regulator, OmpR, which together control the expression of OmpC and OmpF porins in response to the osmolyte concentration...
  52. Hirakawa H, Nishino K, Yamada J, Hirata T, Yamaguchi A. Beta-lactam resistance modulated by the overexpression of response regulators of two-component signal transduction systems in Escherichia coli. J Antimicrob Chemother. 2003;52:576-82 pubmed
    ..Thirteen response regulators out of 32 genes, namely baeR, cheY, cpxR, creB, evgA, fimZ, narL, ompR, rcsB, rstA, yedW, yehT and dcuR, conferred increased beta-lactam resistance...
  53. Matsubara M, Kitaoka S, Takeda S, Mizuno T. Tuning of the porin expression under anaerobic growth conditions by his-to-Asp cross-phosphorelay through both the EnvZ-osmosensor and ArcB-anaerosensor in Escherichia coli. Genes Cells. 2000;5:555-69 pubmed
    ..known that the osmoregulatory profile of the outer membrane porins (OmpC and OmpF) is mainly regulated by the EnvZ-OmpR phosphorelay system in response to medium osmolarity...
  54. Kojetin D, Sullivan D, Thompson R, Cavanagh J. Classification of response regulators based on their surface properties. Methods Enzymol. 2007;422:141-69 pubmed
    ..Our studies of the OmpR subfamily RRs in Bacillus subtilis and Escherichia coli reveal a notable correlation between the RR receiver domain ..
  55. Stock J, Stock A, Mottonen J. Signal transduction in bacteria. Nature. 1990;344:395-400 pubmed publisher
    ..This phosphotransfer network couples environmental signals to an array of response elements that control cell motility and regulate gene expression...
  56. Dupont H, Choinier P, Roche D, Adiba S, Sookdeb M, Branger C, et al. Structural Alteration of OmpR as a Source of Ertapenem Resistance in a CTX-M-15-Producing Escherichia coli O25b:H4 Sequence Type 131 Clinical Isolate. Antimicrob Agents Chemother. 2017;61: pubmed publisher
    ..One nucleotide substitution was identified between these strains. It affected the ompR gene, which codes for a regulatory protein involved in the control of OmpC/OmpF porin expression, creating a ..
  57. Sule P, Wadhawan T, Carr N, Horne S, Wolfe A, PrĂ¼ss B. A combination of assays reveals biomass differences in biofilms formed by Escherichia coli mutants. Lett Appl Microbiol. 2009;49:299-304 pubmed publisher
    ..The ATP assay, in combination with other quantitative and qualitative assays, will allow us to perform genetic studies on the regulatory network that underlies the early steps in E. coli biofilm formation. ..
  58. Mizuno T, Mizushima S. Isolation and characterization of deletion mutants of ompR and envZ, regulatory genes for expression of the outer membrane proteins OmpC and OmpF in Escherichia coli. J Biochem. 1987;101:387-96 pubmed
    ..is known to be controlled by at least two regulatory genes, ompR and envZ, which together comprise a single ompB operon. We constructed chromosomal mutants with either ompR-envZ deletion or envZ deletion...
  59. Levskaya A, Chevalier A, Tabor J, Simpson Z, Lavery L, Levy M, et al. Synthetic biology: engineering Escherichia coli to see light. Nature. 2005;438:441-2 pubmed
    ..This spatial control of bacterial gene expression could be used to 'print' complex biological materials, for example, and to investigate signalling pathways through precise spatial and temporal control of their phosphorylation steps. ..
  60. Graeme Cook K, May G, Bremer E, Higgins C. Osmotic regulation of porin expression: a role for DNA supercoiling. Mol Microbiol. 1989;3:1287-94 pubmed
    ..The pair of regulatory proteins, OmpR and EnvZ, are required for normal porin expression...
  61. Xiao H, Kalman M, Ikehara K, Zemel S, Glaser G, Cashel M. Residual guanosine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations. J Biol Chem. 1991;266:5980-90 pubmed
    ..We favor the possibility that the SpoT protein is a bifunctional enzyme capable of catalyzing either ppGpp synthesis or degradation...
  62. Kondo H, Nakagawa A, Nishihira J, Nishimura Y, Mizuno T, Tanaka I. Escherichia coli positive regulator OmpR has a large loop structure at the putative RNA polymerase interaction site. Nat Struct Biol. 1997;4:28-31 pubmed
    The C-terminal DNA-binding domain of OmpR, a positive regulator involved in osmoregulation expression of the ompF and ompC genes in Escherichia coli, has a helix-turn-helix variant motif...
  63. Matsuyama S, Mizuno T, Mizushima S. Interaction between two regulatory proteins in osmoregulatory expression of ompF and ompC genes in Escherichia coli: a novel ompR mutation suppresses pleiotropic defects caused by an envZ mutation. J Bacteriol. 1986;168:1309-14 pubmed
    The ompR and envZ genes, which together constitute the ompB operon, are involved in osmoregulatory expression of the OmpF and OmpC proteins, major outer membrane proteins of Escherichia coli...
  64. Schweizer H, Grussenmeyer T, Boos W. Mapping of two ugp genes coding for the pho regulon-dependent sn-glycerol-3-phosphate transport system of Escherichia coli. J Bacteriol. 1982;150:1164-71 pubmed
    ..It is either the structural gene for the binding protein or located proximal to it. The analysis of the crosses allowed the ordering of the markers in the clockwise direction as follows: aroB, malA, asd, ugpA, ugpB, livH, pit...
  65. Schwan W. Survival of uropathogenic Escherichia coli in the murine urinary tract is dependent on OmpR. Microbiology. 2009;155:1832-9 pubmed publisher
    ..b>OmpR is the response regulator part of a two-component OmpR-EnvZ regulatory system that responds to osmotic stresses...
  66. Gerken H, Charlson E, Cicirelli E, Kenney L, Misra R. MzrA: a novel modulator of the EnvZ/OmpR two-component regulon. Mol Microbiol. 2009;72:1408-22 pubmed publisher
    ..Genetic evidence shows that overexpression of MzrA--formerly known as YqjB and EcfM--modulates the activity of EnvZ/OmpR similarly to pleiotropic EnvZ mutants and alter porin expression...
  67. Giraud A, Arous S, De Paepe M, Gaboriau Routhiau V, Bambou J, Rakotobe S, et al. Dissecting the genetic components of adaptation of Escherichia coli to the mouse gut. PLoS Genet. 2008;4:e2 pubmed publisher
    ..that Escherichia coli MG1655 adapts rapidly to the intestine of germ-free mice by single point mutations in EnvZ/OmpR two-component signal transduction system, which controls more than 100 genes...
  68. Pao G, Saier M. Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution. J Mol Evol. 1995;40:136-54 pubmed
    ..Sequence data were correlated with structure/function when such information was available.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  69. Parkinson J. Signal transduction schemes of bacteria. Cell. 1993;73:857-71 pubmed
  70. Yang Y, Inouye M. Intermolecular complementation between two defective mutant signal-transducing receptors of Escherichia coli. Proc Natl Acad Sci U S A. 1991;88:11057-61 pubmed
    ..and EnvZ, an osmosensor of Escherichia coli that is able to induce ompC expression by phosphorylating OmpR (a transcriptional activator) in response to aspartate...
  71. Mizuno T, Wurtzel E, Inouye M. Cloning of the regulatory genes (ompR and envZ) for the matrix proteins of the Escherichia coli outer membrane. J Bacteriol. 1982;150:1462-6 pubmed
    We have cloned the regulatory gene cluster of Escherichia coli which is composed of at least two distinct genes, ompR and envZ. These genes are known to regulate the production of the outer membrane matrix proteins...
  72. Quinn H, Cameron A, Dorman C. Bacterial regulon evolution: distinct responses and roles for the identical OmpR proteins of Salmonella Typhimurium and Escherichia coli in the acid stress response. PLoS Genet. 2014;10:e1004215 pubmed publisher
    ..For example, the archetypal DNA binding protein, OmpR, is identical between Salmonella Typhimurium serovar Typhimurium and Escherichia coli, but regulatory ..