ompF

Summary

Gene Symbol: ompF
Description: outer membrane porin 1a (Ia;b;F)
Alias: ECK0920, JW0912, cmlB, coa, cry, nfxB?, tolF
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Yamashita E, Zhalnina M, Zakharov S, Sharma O, Cramer W. Crystal structures of the OmpF porin: function in a colicin translocon. EMBO J. 2008;27:2171-80 pubmed publisher
    The OmpF porin in the Escherichia coli outer membrane (OM) is required for the cytotoxic action of group A colicins, which are proposed to insert their translocation and active domains through OmpF pores...
  2. Cowan S, Schirmer T, Rummel G, Steiert M, Ghosh R, Pauptit R, et al. Crystal structures explain functional properties of two E. coli porins. Nature. 1992;358:727-33 pubmed
    ..The structures explain at the molecular level functional characteristics and their alterations by known mutations. ..
  3. Iyer R, Delcour A. Complex inhibition of OmpF and OmpC bacterial porins by polyamines. J Biol Chem. 1997;272:18595-601 pubmed
    ..of four polyamines (putrescine, cadaverine, spermidine, and spermine) on the activity of bacterial porins OmpC and OmpF were investigated by electrophysiology...
  4. Visudtiphole V, Thomas M, Chalton D, Lakey J. Refolding of Escherichia coli outer membrane protein F in detergent creates LPS-free trimers and asymmetric dimers. Biochem J. 2005;392:375-81 pubmed
    The Escherichia coli OmpF (outer-membrane protein F; matrix porin) is a homotrimeric beta-barrel and a member of the bacterial porin superfamily...
  5. Baboolal T, Conroy M, Gill K, Ridley H, Visudtiphole V, Bullough P, et al. Colicin N binds to the periphery of its receptor and translocator, outer membrane protein F. Structure. 2008;16:371-9 pubmed publisher
    ..Colicin N displays an unusually simple translocation pathway, using the outer membrane protein F (OmpF) as both receptor and translocator...
  6. Wu L, Lin X, Peng X. From proteome to genome for functional characterization of pH-dependent outer membrane proteins in Escherichia coli. J Proteome Res. 2009;8:1059-70 pubmed publisher
    ..expressed proteins identified, OstA, TolC, OmpT, OmpP OmpC, Trak, OmpX, Dps, LamB, Tsx, FadL, OmpW, and OmpF were characterized as pH-related OM proteins...
  7. Bourdineaud J, Fierobe H, Lazdunski C, Pages J. Involvement of OmpF during reception and translocation steps of colicin N entry. Mol Microbiol. 1990;4:1737-43 pubmed
    125I]-colicin N binds to OmpF receptor sites (70,000 per cell) with an average Kassoc of 3.2 x 10(6) M-1 at 23 degrees C...
  8. Mizuno T, Mizushima S. Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the porin genes. Mol Microbiol. 1990;4:1077-82 pubmed
    Expression of Escherichia coli outer-membrane porin proteins (OmpF and OmpC) is regulated by the osmolarity of the medium...
  9. Housden N, Hopper J, Lukoyanova N, Rodriguez Larrea D, Wojdyla J, Klein A, et al. Intrinsically disordered protein threads through the bacterial outer-membrane porin OmpF. Science. 2013;340:1570-4 pubmed publisher
    ..E9 (ColE9) assembles a cytotoxic translocon at the surface of Escherichia coli that incorporates the trimeric porin OmpF. Formation of the translocon involved ColE9's unstructured N-terminal domain threading in opposite directions ..

More Information

Publications89

  1. Housden N, Wojdyla J, Korczynska J, Grishkovskaya I, Kirkpatrick N, Brzozowski A, et al. Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF. Proc Natl Acad Sci U S A. 2010;107:21412-7 pubmed publisher
    The porins OmpF and OmpC are trimeric ?-barrel proteins with narrow channels running through each monomer that exclude molecules > 600 Da while mediating the passive diffusion of small nutrients and metabolites across the Gram-negative ..
  2. Zakharov S, Eroukova V, Rokitskaya T, Zhalnina M, Sharma O, Loll P, et al. Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import. Biophys J. 2004;87:3901-11 pubmed
    ..The identity and interaction properties of this OMP were analyzed in planar bilayer experiments.OmpF and TolC channels in planar bilayers were occluded by colicins E3 and E1, respectively, from the trans-side of the ..
  3. Jeanteur D, Schirmer T, Fourel D, Simonet V, Rummel G, Widmer C, et al. Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli. Proc Natl Acad Sci U S A. 1994;91:10675-9 pubmed
    ..on the basis of its resistance to colicin N, reveals distinct structural and functional alterations in unspecific OmpF porin...
  4. Mizuno T, Chou M, Inouye M. A comparative study on the genes for three porins of the Escherichia coli outer membrane. DNA sequence of the osmoregulated ompC gene. J Biol Chem. 1983;258:6932-40 pubmed
    ..The promoter sequence of the ompC gene was compared with that of the ompF gene, both of which are controlled by the osmoregulatory operon, ompB...
  5. Samartzidou H, Delcour A. Excretion of endogenous cadaverine leads to a decrease in porin-mediated outer membrane permeability. J Bacteriol. 1999;181:791-8 pubmed
    ..the mere expression of cadC, in the absence of cadaverine production, leads to a reduction in the amounts of OmpF and OmpC proteins, which suggests a novel mechanism for the environmental control of porin expression...
  6. Kurisu G, Zakharov S, Zhalnina M, Bano S, Eroukova V, Rokitskaya T, et al. The structure of BtuB with bound colicin E3 R-domain implies a translocon. Nat Struct Biol. 2003;10:948-54 pubmed
    ..The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3.
  7. Housden N, Loftus S, Moore G, James R, Kleanthous C. Cell entry mechanism of enzymatic bacterial colicins: porin recruitment and the thermodynamics of receptor binding. Proc Natl Acad Sci U S A. 2005;102:13849-54 pubmed
    ..First, we show that the porin OmpF is recruited directly to the BtuB.colicin complex to form the translocon...
  8. Sharma O, Yamashita E, Zhalnina M, Zakharov S, Datsenko K, Wanner B, et al. Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon. J Biol Chem. 2007;282:23163-70 pubmed
    ..Screening of porin knock-out mutants showed that either OmpF or OmpC can function in such a translocon...
  9. Zakharov S, Zhalnina M, Sharma O, Cramer W. The colicin E3 outer membrane translocon: immunity protein release allows interaction of the cytotoxic domain with OmpF porin. Biochemistry. 2006;45:10199-207 pubmed
    ..Together with genetic analysis and studies on colicin occlusion of OmpF channels, this implied a colicin translocon consisting of BtuB and OmpF that would transfer the C-terminal ..
  10. Freire P, Vieira H, Furtado A, de Pedro M, Arraiano C. Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane. FEMS Microbiol Lett. 2006;260:106-11 pubmed
    ..Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.
  11. Huang K, Igo M. Identification of the bases in the ompF regulatory region, which interact with the transcription factor OmpR. J Mol Biol. 1996;262:615-28 pubmed
    Expression of the outer membrane protein OmpF of Escherichia coli K-12 is influenced by a variety of environmental signals. Most of the signals are thought to regulate OmpF expression at the level of transcription initiation...
  12. Zhang E, Ferenci T. OmpF changes and the complexity of Escherichia coli adaptation to prolonged lactose limitation. FEMS Microbiol Lett. 1999;176:395-401 pubmed
    ..Both structural and regulatory mutations affecting OmpF porin predominated in isolates after 210-280 generations of culture...
  13. Tsui P, Helu V, Freundlich M. Altered osmoregulation of ompF in integration host factor mutants of Escherichia coli. J Bacteriol. 1988;170:4950-3 pubmed
    Osmoregulation of the porin protein OmpF was strongly altered in integration host factor (IHF) mutants. These mutants produced approximately 15-fold more OmpF than did the parent strain when grown in media of intermediate osmolarity...
  14. Vidal S, Bredin J, Pages J, Barbe J. Beta-lactam screening by specific residues of the OmpF eyelet. J Med Chem. 2005;48:1395-400 pubmed
    Beta-lactams use aqueous channels of porins to penetrate Gram-negative bacteria. The L3 loop of Escherichia coli OmpF porin is a key feature that actively contributes to both channel size and electrostatic properties...
  15. Castillo Keller M, Vuong P, Misra R. Novel mechanism of Escherichia coli porin regulation. J Bacteriol. 2006;188:576-86 pubmed
    ..Overexpression of ipeX RNA inhibited both OmpC and OmpF expression but not that of OmpA...
  16. Saint N, Lou K, Widmer C, Luckey M, Schirmer T, Rosenbusch J. Structural and functional characterization of OmpF porin mutants selected for larger pore size. II. Functional characterization. J Biol Chem. 1996;271:20676-80 pubmed
    The effects on the channel characteristics of four single amino acid substitutions in OmpF porin and of a deletion mutant in the constriction loop L3 have been studied...
  17. Schmitt E, Vrouenraets M, Steinem C. Channel activity of OmpF monitored in nano-BLMs. Biophys J. 2006;91:2163-71 pubmed
    ..After lipid bilayers had thinned, the reconstitution and ionic conducting properties of the outer membrane protein OmpF of E. coli were monitored using single-channel recordings...
  18. Phale P, Philippsen A, Kiefhaber T, Koebnik R, Phale V, Schirmer T, et al. Stability of trimeric OmpF porin: the contributions of the latching loop L2. Biochemistry. 1998;37:15663-70 pubmed
    The channel-forming protein OmpF porin from Escherichia coli spans the bacterial outer membrane. Each of the three monomers comprises a hollow, 16-stranded beta-barrel...
  19. Robertson K, Tieleman D. Molecular basis of voltage gating of OmpF porin. Biochem Cell Biol. 2002;80:517-23 pubmed
    b>OmpF and PhoE from Escherichia coli and related homologous proteins from other Gram-negative bacteria allow the passive transport of small polar molecules across the bacterial outer membrane...
  20. Villa R, Lotti M, Gatti Lafranconi P. Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm. Microb Cell Fact. 2009;8:32 pubmed publisher
    ..In contrast, both proteins induced a similar response in the membrane proteome, i.e. increased levels of LamB, OmpF, OmpA and TolC...
  21. Huang K, Schieberl J, Igo M. A distant upstream site involved in the negative regulation of the Escherichia coli ompF gene. J Bacteriol. 1994;176:1309-15 pubmed
    ..system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription...
  22. Molloy M, Herbert B, Walsh B, Tyler M, Traini M, Sanchez J, et al. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis. 1998;19:837-44 pubmed publisher
  23. Henry T, Pommier S, Journet L, Bernadac A, Gorvel J, Lloubes R. Improved methods for producing outer membrane vesicles in Gram-negative bacteria. Res Microbiol. 2004;155:437-46 pubmed
    ..coli or Shigella flexneri cell envelope and to induce production of high amounts of vesicles. This technique was further found to work efficiently in Salmonella enterica serovar Typhimurium. ..
  24. Varma S, Jakobsson E. Ionization states of residues in OmpF and mutants: effects of dielectric constant and interactions between residues. Biophys J. 2004;86:690-704 pubmed
    ..the protonation states at neutral bulk pH of titratable residues lining the lumen of the native Escherichia coli OmpF channel, and five mutants...
  25. Delihas N, Forst S. MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors. J Mol Biol. 2001;313:1-12 pubmed
    ..coli and related bacteria that post-transcriptionally controls expression of the outer membrane porin gene ompF. The micF gene encodes a non-translated 93 nt antisense RNA that binds its target ompF mRNA and regulates ompF ..
  26. Bredin J, Saint N, Malléa M, Dé E, Molle G, Pages J, et al. Alteration of pore properties of Escherichia coli OmpF induced by mutation of key residues in anti-loop 3 region. Biochem J. 2002;363:521-8 pubmed
    The Escherichia coli OmpF pore is governed by an internal constriction consisting of the negatively charged loop 3 folded into the lumen and the positively charged barrel wall located on the opposite side across the pore, 'anti-loop 3'...
  27. Aguilella Arzo M, Garcia Celma J, Cervera J, Alcaraz A, Aguilella V. Electrostatic properties and macroscopic electrodiffusion in OmpF porin and mutants. Bioelectrochemistry. 2007;70:320-7 pubmed
    The bacterial porin OmpF found in the outer membrane of E. coli is a wide channel, characterized by its poor selectivity and almost no ion specificity...
  28. Chen R, Schmidmayr W, Kramer C, Chen Schmeisser U, Henning U. Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1980;77:4592-6 pubmed
    ..The same analysis did not produce evidence for internal homology in the previously determined sequence of outer membrane protein I (porin) nor was any marked resemblance detected between transmembrane proteins I and II. ..
  29. Reeve E. Genetic analysis of some mutations causing resistance to tetracycline in Escherichia coli K12. Genet Res. 1968;11:303-9 pubmed
  30. Inokuchi K, Mutoh N, Matsuyama S, Mizushima S. Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res. 1982;10:6957-68 pubmed
    The nucleotide sequence of the ompF gene coding for a major outer membrane protein of Escherichia coli K-12 has been determined and the amino acid sequence of the OmpF protein was deduced from it...
  31. Matsubara M, Kitaoka S, Takeda S, Mizuno T. Tuning of the porin expression under anaerobic growth conditions by his-to-Asp cross-phosphorelay through both the EnvZ-osmosensor and ArcB-anaerosensor in Escherichia coli. Genes Cells. 2000;5:555-69 pubmed
    ..aerobic growth conditions, it is well known that the osmoregulatory profile of the outer membrane porins (OmpC and OmpF) is mainly regulated by the EnvZ-OmpR phosphorelay system in response to medium osmolarity...
  32. Foulds J. TolF locus in Escherichia coli: chromosomal location and relationship to loci cmlB and tolD. J Bacteriol. 1976;128:604-8 pubmed
    ..It lies between the aroA and pyrD loci at about 21 min on the linkage map of Bachmann et al. (1976). The cmlB locus, determining increased resistance to the antibiotics chloramphenicol and tetracycline, also lies in this ..
  33. Lou K, Saint N, Prilipov A, Rummel G, Benson S, Rosenbusch J, et al. Structural and functional characterization of OmpF porin mutants selected for larger pore size. I. Crystallographic analysis. J Biol Chem. 1996;271:20669-75 pubmed
    b>OmpF porin is a nonspecific pore protein from the outer membrane of Escherichia coli. Previously, a set of mutants was selected that allow the passage of long maltodextrins that do not translocate through the wild-type pore...
  34. Dairi T, Inokuchi K, Mizuno T, Mizushima S. Positive control of transcription initiation in Escherichia coli. A base substitution at the Pribnow box renders ompF expression independent of a positive regulator. J Mol Biol. 1985;184:1-6 pubmed
    Expression of the ompF gene coding for a major outer membrane protein of Escherichia coli is positively regulated by the product of the ompR gene, OmpR...
  35. López M, Aguilella Arzo M, Aguilella V, Alcaraz A. Ion selectivity of a biological channel at high concentration ratio: insights on small ion diffusion and binding. J Phys Chem B. 2009;113:8745-51 pubmed publisher
    The ionic selectivity of the bacterial porin OmpF has been investigated in several chloride salts of alkali metal cations under a wide range of concentration ratios...
  36. Misra R, Reeves P. Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12. J Bacteriol. 1987;169:4722-30 pubmed
    Mutation in the tolC locus greatly reduces normal synthesis of OmpF, a major porin protein of Escherichia coli K-12...
  37. Im W, Roux B. Ions and counterions in a biological channel: a molecular dynamics simulation of OmpF porin from Escherichia coli in an explicit membrane with 1 M KCl aqueous salt solution. J Mol Biol. 2002;319:1177-97 pubmed
    A 5 ns all-atom molecular dynamics trajectory of Escherichia coli OmpF porin embedded in an explicit dimyristoyl-phosphatidylcholine (DMPC) bilayer bathed by a 1 M [KCl] aqueous salt solution is generated to explore the microscopic ..
  38. Nogami T, Mizuno T, Mizushima S. Construction of a series of ompF-ompC chimeric genes by in vivo homologous recombination in Escherichia coli and characterization of the translational products. J Bacteriol. 1985;164:797-801 pubmed
    b>OmpF and OmpC are major outer membrane proteins. Although they are homologous proteins, they function differently in several respects...
  39. Pebay Peyroula E, Garavito R, Rosenbusch J, Zulauf M, Timmins P. Detergent structure in tetragonal crystals of OmpF porin. Structure. 1995;3:1051-9 pubmed
    ..of five porins have been solved by X-ray crystallography including the trigonal crystal form of the trimeric OmpF porin from Escherichia coli...
  40. Basle A, Iyer R, Delcour A. Subconductance states in OmpF gating. Biochim Biophys Acta. 2004;1664:100-7 pubmed
    Discrepancies were noted in the published conductance of the Escherichia coli porin OmpF. Results from various papers are hard to compare because of the use of different channel preparations, salt types and concentrations, and ..
  41. Goh E, Siino D, Igo M. The Escherichia coli tppB (ydgR) gene represents a new class of OmpR-regulated genes. J Bacteriol. 2004;186:4019-24 pubmed
    ..In this study, we examined the expression of a new OmpR-regulated gene, ydgR. Our results indicate that ydgR is equivalent to the Salmonella enterica serovar Typhimurium tppB gene and represents a new class of OmpR-regulated genes. ..
  42. Niramitranon J, Sansom M, Pongprayoon P. Why do the outer membrane proteins OmpF from E. coli and OprP from P. aeruginosa prefer trimers? Simulation studies. J Mol Graph Model. 2016;65:1-7 pubmed publisher
    ..A well-studied OmpF (general porin from Escherichia coli) and well-characterised OprP (phosphate-specific pore from Pseudomonas ..
  43. Molloy M, Herbert B, Slade M, Rabilloud T, Nouwens A, Williams K, et al. Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem. 2000;267:2871-81 pubmed
    ..In an additional demonstration we show the value of parallel protein analysis to document changes in E. coli OMP expression as influenced by culture temperature...
  44. Schabert F, Henn C, Engel A. Native Escherichia coli OmpF porin surfaces probed by atomic force microscopy. Science. 1995;268:92-4 pubmed
    Topographs of two-dimensional porin OmpF crystals reconstituted in the presence of lipids were recorded in solution by atomic force microscopy (AFM) to a lateral resolution of 10 angstroms and a vertical resolution of 1 angstrom...
  45. Traurig M, Misra R. Identification of bacteriophage K20 binding regions of OmpF and lipopolysaccharide in Escherichia coli K-12. FEMS Microbiol Lett. 1999;181:101-8 pubmed
    ..One class of mutants possessed alterations within the ompF gene while the rfa gene cluster, which is responsible for lipopolysaccharide (LPS) synthesis, was affected in the ..
  46. Simonet V, Mallea M, Fourel D, Bolla J, Pages J. Crucial domains are conserved in Enterobacteriaceae porins. FEMS Microbiol Lett. 1996;136:91-7 pubmed
    ..The presence of these common peptides suggested a conservation of specific regions involved in the functional organization of the enterobacterial porins. ..
  47. Bocquet Pages C, Lazdunski C, Lazdunski A. Lipid-synthesis-dependent biosynthesis (or assembly) of major outer-membrane proteins of Escherichia coli. Eur J Biochem. 1981;118:105-11 pubmed
    ..composition of the outer membranes, analyzed after pulse radiolabelling by [35S]methionine, was mainly altered in OmpF and OmpC proteins. These proteins are the main porins used by most nutrients like 5' AMP...
  48. Phale P, Philippsen A, Widmer C, Phale V, Rosenbusch J, Schirmer T. Role of charged residues at the OmpF porin channel constriction probed by mutagenesis and simulation. Biochemistry. 2001;40:6319-25 pubmed
    The channel constriction of OmpF porin, a pore protein in the bacterial outer membrane, is highly charged due to the presence of three arginines (R42, R82, and R132) and two acidic residues (D113 and E117)...
  49. Misra R, CastilloKeller M, Deng M. Overexpression of protease-deficient DegP(S210A) rescues the lethal phenotype of Escherichia coli OmpF assembly mutants in a degP background. J Bacteriol. 2000;182:4882-8 pubmed
    Replacement of OmpF's conserved carboxy-terminal phenylalanine with dissimilar amino acids severely impaired its assembly into stable trimers...
  50. Efremov R, Sazanov L. Structure of Escherichia coli OmpF porin from lipidic mesophase. J Struct Biol. 2012;178:311-8 pubmed publisher
    ..Due to ease of its purification and crystallization OmpF can be used as a benchmark protein for establishing membrane protein crystallization in meso, as a "membrane ..
  51. Burgess N, Dao T, Stanley A, Fleming K. Beta-barrel proteins that reside in the Escherichia coli outer membrane in vivo demonstrate varied folding behavior in vitro. J Biol Chem. 2008;283:26748-58 pubmed publisher
    ..OmpT, outer membrane phospholipase A (OmpLa), the fadl gene product (FadL), the yaet gene product (Omp85), and OmpF. These proteins fold into the same bilayer in vivo and share a transmembrane beta-barrel motif but vary in sequence ..
  52. Malcolm B, Kirsch J. Site-directed mutagenesis of aspartate aminotransferase from E. coli. Biochem Biophys Res Commun. 1985;132:915-21 pubmed
    ..The mutant gene was expressed under control of the Tac promoter to overproduce a mutant protein lacking enzymatic activity. ..
  53. Kreir M, Farre C, Beckler M, George M, Fertig N. Rapid screening of membrane protein activity: electrophysiological analysis of OmpF reconstituted in proteoliposomes. Lab Chip. 2008;8:587-95 pubmed publisher
    ..g. proteins from organelles. In the presented work, we show the example of the outer membrane protein OmpF from Escherichia coli...
  54. Lambert P. Enterobacteriaceae: composition, structure and function of the cell envelope. Soc Appl Bacteriol Symp Ser. 1988;17:21S-34S pubmed
  55. Nurminen M, Hirvas L, Vaara M. The outer membrane of lipid A-deficient Escherichia coli mutant LH530 has reduced levels of OmpF and leaks periplasmic enzymes. Microbiology. 1997;143 ( Pt 5):1533-7 pubmed
    ..Here we show that at 37 degrees C LH530 contains a reduced amount of the OmpF porin and that it leaks periplasmic beta-lactamase at 37 degrees C and 42 degrees C...
  56. Ramani N, Huang L, Freundlich M. In vitro interactions of integration host factor with the ompF promoter-regulatory region of Escherichia coli. Mol Gen Genet. 1992;231:248-55 pubmed
    ..work has shown that integration host factor (IHF) mutants have increased expression and altered osmoregulation of OmpF, a major Escherichia coli outer membrane protein...
  57. Mutoh N, Inokuchi K, Mizushima S. Amino acid sequence of the signal peptide of OmpF, a major outer membrane protein of Escherichia coli. FEBS Lett. 1982;137:171-4 pubmed
  58. Asako H, Kobayashi K, Aono R. Organic solvent tolerance of Escherichia coli is independent of OmpF levels in the membrane. Appl Environ Microbiol. 1999;65:294-6 pubmed
    The organic solvent tolerance of Escherichia coli was measured under conditions in which OmpF levels were controlled by various means as follows: alteration of NaCl concentration in the medium, transformation with a stress-responsive gene ..
  59. Zakharov S, Cramer W. On the mechanism and pathway of colicin import across the E. Coli outer membrane. Front Biosci. 2004;9:1311-7 pubmed
    ..Together with spectroscopic data on the R135-BtuB interaction and electrophysiological data on the colicin E3-OmpF interaction, this has led to a proposal for the utilization of two receptors, BtuB-OmpF, in an outer membrane ..
  60. Sato T, Yura T. Regulatory mutations conferring constitutive synthesis of major outer membrane proteins (OmpC and OmpF) in Escherichia coli. J Bacteriol. 1981;145:88-96 pubmed
    An ompB strain of Escherichia coli K-12 lacking major outer membrane proteins OmpC and OmpF was used to isolate a pair of mutants that have restored the ability to synthesize either OmpC or OmpF protein...
  61. Rosner J, Chai T, Foulds J. Regulation of ompF porin expression by salicylate in Escherichia coli. J Bacteriol. 1991;173:5631-8 pubmed
    The expression of ompF, the gene encoding a major outer membrane protein of Escherichia coli, is regulated by various environmental factors...
  62. Jeanteur D, Lakey J, Pattus F. The bacterial porin superfamily: sequence alignment and structure prediction. Mol Microbiol. 1991;5:2153-64 pubmed
    ..Using the constraints of beta-sheet structure these porins are predicted to contain 16 membrane-spanning strands, 14 of which are common to the two (enteric and the neisserial) porin subfamilies. ..
  63. Chagneau C, Heyde M, Alonso S, Portalier R, Laloi P. External-pH-dependent expression of the maltose regulon and ompF gene in Escherichia coli is affected by the level of glycerol kinase, encoded by glpK. J Bacteriol. 2001;183:5675-83 pubmed
    ..Thus, GlpK-dependent repression of the maltose system requires the cAMP-CRP complex. We propose that the pHo may affect a complex interplay between GlpK, the phosphotransferase-mediated uptake of glucose, and the adenylate cyclase...
  64. Yoshida T, Qin L, Egger L, Inouye M. Transcription regulation of ompF and ompC by a single transcription factor, OmpR. J Biol Chem. 2006;281:17114-23 pubmed
    The ompF and ompC genes of Escherichia coli are reciprocally regulated by a single transcription factor, phosphorylated OmpR (OmpR-P), depending upon medium osmolarity...
  65. Batchelor E, Walthers D, Kenney L, Goulian M. The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC. J Bacteriol. 2005;187:5723-31 pubmed
    ..transposon mutagenesis of a two-color fluorescent reporter strain to identify new regulators of the porin genes ompF and ompC in Escherichia coli...
  66. Pratt L, Silhavy T. Crl stimulates RpoS activity during stationary phase. Mol Microbiol. 1998;29:1225-36 pubmed
    ..We found that lesions in crl, like lesions in rpoS, cause increased transcription of ompF during stationary phase...
  67. Verhoef C, Lugtenberg B, van Boxtel R, de Graaff P, Verheij H. Genetics and biochemistry of the peptidoglycan-associated proteins b and c of Escherichia coli K12. Mol Gen Genet. 1979;169:137-46 pubmed
    ..Genetic analysis showed three linkage groups, corresponding with the genes tolF (phenotype b-c+), meoA (phenotype b+c-) and ompB (phenotypes b-c-, b-c+, b++c- and b++c+/-)...
  68. Alcaraz A, Nestorovich E, López M, García Giménez E, Bezrukov S, Aguilella V. Diffusion, exclusion, and specific binding in a large channel: a study of OmpF selectivity inversion. Biophys J. 2009;96:56-66 pubmed publisher
    We find that moderate cationic selectivity of the general bacterial porin OmpF in sodium and potassium chloride solutions is inversed to anionic selectivity in concentrated solutions of barium, calcium, nickel, and magnesium chlorides...
  69. Zhu Y, Guo T, Park J, Li X, Meng W, Datta A, et al. Elucidating in vivo structural dynamics in integral membrane protein by hydroxyl radical footprinting. Mol Cell Proteomics. 2009;8:1999-2010 pubmed publisher
    ..membrane proteins identified, we investigated the structure details of a typical beta-barrel structure, the porin OmpF. We found that six modified tryptic peptides of OmpF were reproducibly detected with 19 amino acids modified under ..
  70. Petersen L, Bollback J, Dimmic M, Hubisz M, Nielsen R. Genes under positive selection in Escherichia coli. Genome Res. 2007;17:1336-43 pubmed
    ..Based on structural evidence, we hypothesize that the selection acting on transposases is related to the genomic conflict between transposable elements and the host genome. ..
  71. Samartzidou H, Delcour A. Distinct sensitivities of OmpF and PhoE porins to charged modulators. FEBS Lett. 1999;444:65-70 pubmed
    The inhibition of the anion-selective PhoE porin by ATP and of the cation-selective OmpF porin by polyamines has been previously documented...
  72. Saint Ruf C, Taddei F, Matic I. Stress and survival of aging Escherichia coli rpoS colonies. Genetics. 2004;168:541-6 pubmed
    ..By measuring cell viability and by transcriptome analysis, here we show that rpoS cells as well as wild-type cells survive when they form colonies on solid media. ..
  73. Nikaido H. Outer membrane barrier as a mechanism of antimicrobial resistance. Antimicrob Agents Chemother. 1989;33:1831-6 pubmed
  74. Chaptal V, Kilburg A, Flot D, Wiseman B, Aghajari N, Jault J, et al. Two different centered monoclinic crystals of the E. coli outer-membrane protein OmpF originate from the same building block. Biochim Biophys Acta. 2016;1858:326-32 pubmed publisher
    ..Here we report the structure of the Escherichia coli outer membrane porin OmpF in two centered monoclinic space groups...
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    ..Expression of ompF was more than 40-fold higher under glucose limitation than under nitrogen (ammonia) limitation in chemostat ..
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    ..In this mutant, a decreased amount of OmpF porin contributes to the lowered drug susceptibility, with a greater effect at 26 degrees C than at 37 degrees C.
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    ..Here we reconstituted single OmpF trimers from the outer membrane of Escherichia coli (E. coli) into a planar lipid bilayer...
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    This paper describes a novel genetic method used to isolate mutations that alter proper assembly of OmpF in the outer membrane...
  79. Misra R. A novel ompC mutation of Escherichia coli K-12 that reduces OmpC and OmpF levels in the outer membrane. Mol Microbiol. 1993;10:1029-35 pubmed
    ..mutation was isolated that not only lowered the amount of its own product, OmpC27, but also reduced the level of OmpF present in the outer membrane...
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    ..protein S-OmpR hybrid protein, we demonstrated that six OmpR molecules bind tandemly to the -100 to -39 sequence of ompF. This sequence consists of three 20-base pair units: F1, F2, and F3, each of which is bound by two OmpR proteins...