Gene Symbol: ompA
Description: outer membrane protein A (3a;II*;G;d)
Alias: ECK0948, JW0940, con, tolG, tut
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Power M, Ferrari B, Littlefield Wyer J, Gordon D, Slade M, Veal D. A naturally occurring novel allele of Escherichia coli outer membrane protein A reduces sensitivity to bacteriophage. Appl Environ Microbiol. 2006;72:7930-2 pubmed
    A novel Escherichia coli outer membrane protein A (OmpA) was discovered through a proteomic investigation of cell surface proteins...
  2. Lee H, Bernstein H. The targeting pathway of Escherichia coli presecretory and integral membrane proteins is specified by the hydrophobicity of the targeting signal. Proc Natl Acad Sci U S A. 2001;98:3471-6 pubmed
    ..have demonstrated that presecretory proteins such as maltose binding protein (MBP) and outer membrane protein A (OmpA) are targeted to the Escherichia coli inner membrane by the molecular chaperone SecB, but that integral membrane ..
  3. Charlson E, Werner J, Misra R. Differential effects of yfgL mutation on Escherichia coli outer membrane proteins and lipopolysaccharide. J Bacteriol. 2006;188:7186-94 pubmed
    ..Without YfgL, the levels of OmpA, OmpF, and LamB are significantly reduced, while OmpC levels are slightly reduced...
  4. Johansen J, Rasmussen A, Overgaard M, Valentin Hansen P. Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins. J Mol Biol. 2006;364:1-8 pubmed
    ..Recently MicA was shown to act in destabilizing the ompA transcript when rapidly grown cells entered the stationary phase of growth...
  5. Beck E, Bremer E. Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12. Nucleic Acids Res. 1980;8:3011-27 pubmed
    A nucleotide sequence of 2271 basepairs has been determined from cloned E. coli DNA which contains ompA. Withing that sequence, starting at nucleotide 1037, an open translational reading frame encodes a protein of 367 amino acids which ..
  6. Douchin V, Bohn C, Bouloc P. Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coli. J Biol Chem. 2006;281:12253-9 pubmed
    ..We used the property that small RNAs are often involved in RNA-RNA interactions to capture RseX putative partners; ompA and ompC mRNA, which encode two major outer membrane proteins, were identified...
  7. Moll I, Afonyushkin T, Vytvytska O, Kaberdin V, Bl si U. Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs. RNA. 2003;9:1308-14 pubmed
    ..More recent work suggested a role of Hfq in cellular physiology through its interaction with ompA mRNA and small RNAs (sRNAs), some of which are involved in translational regulation...
  8. Udekwu K, Darfeuille F, Vogel J, Reimegård J, Holmqvist E, Wagner E. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes Dev. 2005;19:2355-66 pubmed
    This paper shows that the small RNA MicA (previously SraD) is an antisense regulator of ompA in Escherichia coli. MicA accumulates upon entry into stationary phase and down-regulates the level of ompA mRNA...
  9. Rasmussen A, Eriksen M, Gilany K, Udesen C, Franch T, Petersen C, et al. Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control. Mol Microbiol. 2005;58:1421-9 pubmed
    The Escherichia coli ompA mRNA, encoding a highly abundant outer membrane protein, has served as a model for regulated mRNA decay in bacteria...

More Information


  1. Emory S, Belasco J. The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency. J Bacteriol. 1990;172:4472-81 pubmed
    The 5' untranslated region (UTR) of the long-lived Escherichia coli ompA message can function in vivo as an mRNA stabilizer...
  2. Orme R, Douglas C, Rimmer S, Webb M. Proteomic analysis of Escherichia coli biofilms reveals the overexpression of the outer membrane protein OmpA. Proteomics. 2006;6:4269-77 pubmed we have used proteomic analysis coupled with immunoassays to show that the major outer membrane protein (OmpA) of Escherichia coli is overexpressed during biofilm formation...
  3. Garten W, Hindennach I, Henning U. The major proteins of the Escherichia coli outer cell envelope membrane. Characterization of proteins II* and III, comparison of all proteins. Eur J Biochem. 1975;59:215-21 pubmed
    ..There are not more major proteins than these. All four proteins are compared and discussed regarding their unknown functions and their relation to E. coli outer membrane proteins studied by other authors. ..
  4. Boy D, Koch H. Visualization of distinct entities of the SecYEG translocon during translocation and integration of bacterial proteins. Mol Biol Cell. 2009;20:1804-15 pubmed publisher
    ..These results suggest that the assembly or the stability of the bacterial SecYEG translocon is influenced by the substrate that needs to be transported. ..
  5. Datta D, Arden B, Henning U. Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors. J Bacteriol. 1977;131:821-9 pubmed
    ..In addition, the association of proteins Ia and Ib with the murein layer of the cell envelope makes this pair trans-membrane proteins. ..
  6. Yi L, Jiang F, Chen M, Cain B, Bolhuis A, Dalbey R. YidC is strictly required for membrane insertion of subunits a and c of the F(1)F(0)ATP synthase and SecE of the SecYEG translocase. Biochemistry. 2003;42:10537-44 pubmed
    ..In contrast, we found that protein export of OmpA is severely blocked at 25 degrees C when YidC is depleted, which may be due to the decreased SecE level, as a 50% ..
  7. Kleinschmidt J, Tamm L. Secondary and tertiary structure formation of the beta-barrel membrane protein OmpA is synchronized and depends on membrane thickness. J Mol Biol. 2002;324:319-30 pubmed
    ..insertion and folding of a beta-barrel membrane protein has been studied using the outer membrane protein A (OmpA) as an example...
  8. Nouwen N, Berrelkamp G, Driessen A. Charged amino acids in a preprotein inhibit SecA-dependent protein translocation. J Mol Biol. 2009;386:1000-10 pubmed publisher
    ..These results indicate that both clusters of positively and negatively charged amino acids are poor substrates for the Sec translocase and that this is reflected by their inability to stimulate the ATPase activity of SecA. ..
  9. Friemann R, Larsson D, Wang Y, van der Spoel D. Molecular dynamics simulations of a membrane protein-micelle complex in vacuo. J Am Chem Soc. 2009;131:16606-7 pubmed publisher
  10. Hong H, Szabo G, Tamm L. Electrostatic couplings in OmpA ion-channel gating suggest a mechanism for pore opening. Nat Chem Biol. 2006;2:627-35 pubmed
    ..The gate of the OmpA channel is formed by the central Glu52-Arg138 salt bridge, which can open to form alternate ion pairs with Lys82 ..
  11. Hedges R, Shannon K. Resistance to apramycin in Escherichia coli isolated from animals: detection of a novel aminoglycoside-modifying enzyme. J Gen Microbiol. 1984;130:473-82 pubmed
    ..Synthesis of this enzyme was specified by a chromosomal gene located near pyrD at about 20 min on the map of the E. coli K12 chromosome. ..
  12. Fernandez C, Hilty C, Bonjour S, Adeishvili K, Pervushin K, Wuthrich K. Solution NMR studies of the integral membrane proteins OmpX and OmpA from Escherichia coli. FEBS Lett. 2001;504:173-8 pubmed
    ..are discussed and illustrated with results obtained with the Escherichia coli integral membrane proteins OmpX and OmpA in mixed micelles with the detergent dihexanoylphosphatidylcholine (DHPC)...
  13. Vytvytska O, Moll I, Kaberdin V, von Gabain A, Blasi U. Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding. Genes Dev. 2000;14:1109-18 pubmed
    ..The Escherichia coli ompA mRNA has served as one of the paradigms for regulated mRNA decay in prokaryotes...
  14. Udekwu K, Wagner E. Sigma E controls biogenesis of the antisense RNA MicA. Nucleic Acids Res. 2007;35:1279-88 pubmed by us and others showed that the antisense RNA MicA downregulates the synthesis of the outer membrane protein OmpA upon entry into stationary phase...
  15. Bol R, de Wit J, Driessen A. The active protein-conducting channel of Escherichia coli contains an apolar patch. J Biol Chem. 2007;282:29785-93 pubmed
    ..Fluorescence measurements with defined proOmpA-DHFR translocation intermediates indicate mostly a water-exposed environment with a hydrophobic region in the center of the channel. ..
  16. Afonyushkin T, Moll I, Blasi U, Kaberdin V. Temperature-dependent stability and translation of Escherichia coli ompA mRNA. Biochem Biophys Res Commun. 2003;311:604-9 pubmed
    RNase E is known to affect the turnover of ompA mRNA in a growth rate-dependent manner...
  17. Henry T, Pommier S, Journet L, Bernadac A, Gorvel J, Lloubes R. Improved methods for producing outer membrane vesicles in Gram-negative bacteria. Res Microbiol. 2004;155:437-46 pubmed
    ..coli or Shigella flexneri cell envelope and to induce production of high amounts of vesicles. This technique was further found to work efficiently in Salmonella enterica serovar Typhimurium. ..
  18. Smith S, Mahon V, Lambert M, Fagan R. A molecular Swiss army knife: OmpA structure, function and expression. FEMS Microbiol Lett. 2007;273:1-11 pubmed
    The OmpA outer membrane protein of Escherichia coli and other enterobacteria is a multifaceted protein. This protein is expressed to very high levels and ompA is tightly regulated at the posttranscriptional level...
  19. Chen R, Schmidmayr W, Kramer C, Chen Schmeisser U, Henning U. Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1980;77:4592-6 pubmed
    The amino acid sequence of major outer membrane protein II (ompA protein) from Escherichia coli K-12 has been determined. The transmembrane polypeptide consists of 325 residues, resulting in a molecular weight of 35,159...
  20. Doerrler W, Raetz C. Loss of outer membrane proteins without inhibition of lipid export in an Escherichia coli YaeT mutant. J Biol Chem. 2005;280:27679-87 pubmed
    ..By Western blotting, we show that levels of the major OM protein OmpA are lower in the mutant in whole cells, membranes, and the growth medium...
  21. Kleinschmidt J, Den Blaauwen T, Driessen A, Tamm L. Outer membrane protein A of Escherichia coli inserts and folds into lipid bilayers by a concerted mechanism. Biochemistry. 1999;38:5006-16 pubmed
    Unfolded outer membrane protein A (OmpA) of Escherichia coli spontaneously inserts and refolds into lipid bilayers upon dilution of denaturing urea...
  22. Qu J, Behrens Kneip S, Holst O, Kleinschmidt J. Binding regions of outer membrane protein A in complexes with the periplasmic chaperone Skp. A site-directed fluorescence study. Biochemistry. 2009;48:4926-36 pubmed publisher
    ..We have examined the binding sites of outer membrane protein A (OmpA) from Escherichia coli in its complexes with the membrane protein chaperone Skp and with Skp and lipopolysaccharide ..
  23. Andrade J, Cairrao F, Arraiano C. RNase R affects gene expression in stationary phase: regulation of ompA. Mol Microbiol. 2006;60:219-28 pubmed
    ..demonstrate that RNase R is induced in stationary phase and is involved in the post-transcriptional regulation of ompA mRNA. This work is the first report of RNase R activity on a full length mRNA...
  24. Fu H, Belaaouaj A, Dahlgren C, Bylund J. Outer membrane protein A deficient Escherichia coli activates neutrophils to produce superoxide and shows increased susceptibility to antibacterial peptides. Microbes Infect. 2003;5:781-8 pubmed
    The outer membrane protein A (OmpA) of Gram-negative bacteria has been ascribed multiple functions including maintenance of structural membrane integrity and porin activity...
  25. Eboigbodin K, Ojeda J, Biggs C. Investigating the surface properties of Escherichia coli under glucose controlled conditions and its effect on aggregation. Langmuir. 2007;23:6691-7 pubmed
    ..Our findings therefore revealed that glucose limits aggregation in E. coli MG1655 by altering the concentration of functional groups from macromolecules present on the bacterial surface. ..
  26. Hong H, Rinehart D, Tamm L. Membrane depth-dependent energetic contribution of the tryptophan side chain to the stability of integral membrane proteins. Biochemistry. 2013;52:4413-21 pubmed publisher
    ..depths in the bilayer contribute to the stability of integral membrane proteins using outer membrane protein A (OmpA) as a model...
  27. Molloy M, Herbert B, Walsh B, Tyler M, Traini M, Sanchez J, et al. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis. 1998;19:837-44 pubmed publisher
  28. Peterson J, Woolhead C, Bernstein H. Basic amino acids in a distinct subset of signal peptides promote interaction with the signal recognition particle. J Biol Chem. 2003;278:46155-62 pubmed
    ..acids in a signal peptide influence the targeting of two Escherichia coli proteins, maltose binding protein and OmpA. Whereas both proteins are normally targeted to the inner membrane by SecB, we found that replacement of their ..
  29. Gophna U, Ideses D, Rosen R, Grundland A, Ron E. OmpA of a septicemic Escherichia coli O78--secretion and convergent evolution. Int J Med Microbiol. 2004;294:373-81 pubmed
    b>OmpA is an important constituent of the outer membrane of Gram-negative bacteria. OmpA is involved in a variety of host-bacteria interactions, including crossing of the blood-brain barrier by E...
  30. Ried G, Henning U. A unique amino acid substitution in the outer membrane protein OmpA causes conjugation deficiency in Escherichia coli K-12. FEBS Lett. 1987;223:387-90 pubmed
    The outer membrane protein OmpA of E. coli K-12 can serve as a receptor for phages and is required for stabilizing mating aggregates during F'-mediated conjugation...
  31. Pascal T, Abrol R, Mittal R, Wang Y, Prasadarao N, Goddard W. Experimental validation of the predicted binding site of Escherichia coli K1 outer membrane protein A to human brain microvascular endothelial cells: identification of critical mutations that prevent E. coli meningitis. J Biol Chem. 2010;285:37753-61 pubmed publisher interact with GlcNAc1-4GlcNAc epitopes of Ecgp96 on human brain microvascular endothelial cells (HBMECs) via OmpA (outer membrane protein A)...
  32. Kleinschmidt J, Tamm L. Folding intermediates of a beta-barrel membrane protein. Kinetic evidence for a multi-step membrane insertion mechanism. Biochemistry. 1996;35:12993-3000 pubmed
    ..We have used the beta-barrel outer membrane protein A (OmpA) of Escherichia coli as a model to study the kinetics of folding and insertion into dioleoylphosphatidylcholine (..
  33. Urban J, Vogel J. Translational control and target recognition by Escherichia coli small RNAs in vivo. Nucleic Acids Res. 2007;35:1018-37 pubmed
    ..g. despite the fact that ompA fusion mRNA decay could no longer be promoted by MicA. This is the first study in which multiple well-defined E...
  34. Lin H, Hsu C, Yang C, Ju Y, Chen Y, Tseng C. Negative effect of glucose on ompA mRNA stability: a potential role of cyclic AMP in the repression of hfq in Escherichia coli. J Bacteriol. 2011;193:5833-40 pubmed publisher
    ..In the present study, we found that the stability of ompA mRNA was reduced in Escherichia coli when glucose (40 mM) was present in Luria-Bertani (LB) medium...
  35. Klose M, Schwarz H, Macintyre S, Freudl R, Eschbach M, Henning U. Internal deletions in the gene for an Escherichia coli outer membrane protein define an area possibly important for recognition of the outer membrane by this polypeptide. J Biol Chem. 1988;263:13291-6 pubmed
    A series of overlapping deletions has been constructed in the ompA gene which encodes the 325-residue Escherichia coli outer membrane protein OmpA...
  36. Kleinschmidt J. Membrane protein folding on the example of outer membrane protein A of Escherichia coli. Cell Mol Life Sci. 2003;60:1547-58 pubmed publisher
    ..insert and fold into a biomembrane have mostly been studied with bacteriorhodopsin and outer membrane protein A (OmpA)...
  37. Le Brun A, Holt S, Shah D, Majkrzak C, Lakey J. Monitoring the assembly of antibody-binding membrane protein arrays using polarised neutron reflection. Eur Biophys J. 2008;37:639-45 pubmed publisher
    ..The scaffold protein is a fusion of the monomeric E. coli porin outer membrane protein A (OmpA) and the Z domain of Staphylococcus aureus protein A...
  38. Movva N, Nakamura K, Inouye M. Regulatory region of the gene for the ompA protein, a major outer membrane protein of Escherichia coli. Proc Natl Acad Sci U S A. 1980;77:3845-9 pubmed
    The ompA protein, an outer membrane protein required for conjugation, is one of the most abundant proteins in Escherichia coli...
  39. Mohanty B, Kushner S. The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells. Nucleic Acids Res. 2006;34:5695-704 pubmed
    ..PCR analysis indicates that the extent of polyadenylation of individual full-length transcripts such as lpp and ompA varies significantly in wild-type cells. The data presented here demonstrates that polyadenylation in E...
  40. Ye C, Chai Q, Zhong M, Wei Y. Effect of crowding by Ficolls on OmpA and OmpT refolding and membrane insertion. Protein Sci. 2013;22:239-45 pubmed publisher
    ..Using OmpA and OmpT as model proteins and Ficoll 70 as the crowding agent, here we investigated the effect of the ..
  41. van Alphen L, Havekes L, Lugtenberg B. Major outer membrane protein d of Escherichia coli K12. Purification and in vitro activity of bacteriophages k3 and f-pilus mediated conjugation. FEBS Lett. 1977;75:285-90 pubmed
  42. Gromiha M, Ponnuswamy P. Prediction of transmembrane beta-strands from hydrophobic characteristics of proteins. Int J Pept Protein Res. 1993;42:420-31 pubmed
    ..The predicted beta-structure contents in OmpA, porin from E. coli and maltoporin compared with the Raman spectroscopic results at 95% level...
  43. Zakharian E, Reusch R. Kinetics of folding of Escherichia coli OmpA from narrow to large pore conformation in a planar bilayer. Biochemistry. 2005;44:6701-7 pubmed
    The outer membrane protein of Escherichia coli, OmpA, is currently alleged to adopt two native conformations: a major two-domain conformer in which 171 N-terminal residues form a narrow eight beta-barrel pore and 154 C-terminal residues ..
  44. Danoff E, Fleming K. The soluble, periplasmic domain of OmpA folds as an independent unit and displays chaperone activity by reducing the self-association propensity of the unfolded OmpA transmembrane ?-barrel. Biophys Chem. 2011;159:194-204 pubmed publisher
    b>OmpA is one of only a few transmembrane proteins whose folding and stability have been investigated in detail...
  45. Khan N, Shin S, Chung J, Kim K, Elliott S, Wang Y, et al. Outer membrane protein A and cytotoxic necrotizing factor-1 use diverse signaling mechanisms for Escherichia coli K1 invasion of human brain microvascular endothelial cells. Microb Pathog. 2003;35:35-42 pubmed
    ..We previously have shown that outer membrane protein A (OmpA) and cytotoxic necrotizing factor-1 (CNF1) contribute to E. coli K1 invasion of BMEC...
  46. Burgess N, Dao T, Stanley A, Fleming K. Beta-barrel proteins that reside in the Escherichia coli outer membrane in vivo demonstrate varied folding behavior in vitro. J Biol Chem. 2008;283:26748-58 pubmed publisher
    ..We cloned, expressed, and folded nine OMPs: outer membrane protein X (OmpX), OmpW, OmpA, the crcA gene product (PagP), OmpT, outer membrane phospholipase A (OmpLa), the fadl gene product (FadL), the yaet ..
  47. Lelong C, Rolland M, Louwagie M, Garin J, Geiselmann J. Mutual regulation of Crl and Fur in Escherichia coli W3110. Mol Cell Proteomics. 2007;6:660-8 pubmed
    ..Crl modulated the affinity of Fur at the fur promoter but not at the crl promoter. The triad RpoS-Crl-Fur may thus represent the centerpiece of a global regulatory system of response to different stresses. ..
  48. Qu J, Mayer C, Behrens S, Holst O, Kleinschmidt J. The trimeric periplasmic chaperone Skp of Escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions. J Mol Biol. 2007;374:91-105 pubmed
    ..We have examined the binding of Skp to various OMPs of different origin, size, and function. These were OmpA, OmpG, and YaeT (Omp85) from Escherichia coli, the translocator domain of the autotransporter NalP from Neisseria ..
  49. Burmann B, Wang C, Hiller S. Conformation and dynamics of the periplasmic membrane-protein-chaperone complexes OmpX-Skp and tOmpA-Skp. Nat Struct Mol Biol. 2013;20:1265-72 pubmed publisher
    ..The dynamic state allows for energy-independent substrate release and provides a general paradigm for the conformation of OMP polypeptides bound to energy-independent chaperones...
  50. Liang F, Bageshwar U, Musser S. Bacterial Sec protein transport is rate-limited by precursor length: a single turnover study. Mol Biol Cell. 2009;20:4256-66 pubmed publisher
    ..Instead, we propose that precursor movement results predominantly from Brownian motion and that the SecA ATPase regulates pore accessibility. ..
  51. Danoff E, Fleming K. Novel Kinetic Intermediates Populated along the Folding Pathway of the Transmembrane β-Barrel OmpA. Biochemistry. 2017;56:47-60 pubmed publisher
    We examined the folding of the β-barrel membrane protein OmpA from Escherichia coli...
  52. Tomkiewicz D, Nouwen N, van Leeuwen R, Tans S, Driessen A. SecA supports a constant rate of preprotein translocation. J Biol Chem. 2006;281:15709-13 pubmed
    ..These data indicate that in the ATP-dependent reaction, SecA drives a constant rate of preprotein translocation consistent with a stepping mechanism of translocation. ..
  53. Deana A, Ehrlich R, Reiss C. Synonymous codon selection controls in vivo turnover and amount of mRNA in Escherichia coli bla and ompA genes. J Bacteriol. 1996;178:2718-20 pubmed
    A number of silent codon changes were made in two Escherichia coli genes. For the ompA gene, the replacement of seven consecutive frequently used codons with synonymous infrequently used codons reduced the ompA mRNA level and its half-..
  54. Clavel T, Germon P, Vianney A, Portalier R, Lazzaroni J. TolB protein of Escherichia coli K-12 interacts with the outer membrane peptidoglycan-associated proteins Pal, Lpp and OmpA. Mol Microbiol. 1998;29:359-67 pubmed
    ..It was shown that TolB could be cross-linked in vivo with Pal, OmpA and Lpp, while Pal was associated with TolB and OmpA...
  55. Shi -, Wen Su W -. Display of green fluorescent protein on Escherichia coli cell surface. Enzyme Microb Technol. 2001;28:25-34 pubmed
    ..GFP) on the external surface of Escherichia coli was achieved by construction of a fusion protein between Lpp-OmpA hybrid and a GFP variant, GFPmut2...
  56. Wang Y. The function of OmpA in Escherichia coli. Biochem Biophys Res Commun. 2002;292:396-401 pubmed
    Outer membrane protein A (OmpA) is a major protein in the Escherichia coli outer membrane. In this study, the function of OmpA in E. coli stress survival was examined. An E...
  57. Bond P, Cuthbertson J, Deol S, Sansom M. MD simulations of spontaneous membrane protein/detergent micelle formation. J Am Chem Soc. 2004;126:15948-9 pubmed
    ..formation around representatives of the two major families of membrane proteins, a small beta-barrel protein, OmpA, and a model alpha-helical protein, glycophorin A...
  58. Ueno H, Yonesaki T. Phage-induced change in the stability of mRNAs. Virology. 2004;329:134-41 pubmed publisher
    ..Stable E. coli mRNAs such as lpp and ompA were drastically destabilized immediately after infection...
  59. Wang J, Chao Y. Immobilization of cells with surface-displayed chitin-binding domain. Appl Environ Microbiol. 2006;72:927-31 pubmed
    ..ChBD)-based cell immobilization, a tripartite gene fusion consisting of an in-frame fusion of ChBD to lpp and ompA was constructed and expressed in Escherichia coli...
  60. Cole S, Honore N. Transcription of the sulA-ompA region of Escherichia coli during the SOS response and the role of an antisense RNA molecule. Mol Microbiol. 1989;3:715-22 pubmed
    The transcriptional pattern of the 22 min region of the Escherichia coli chromosome containing the linked sulA and ompA genes, which encode an SOS-inducible inhibitor of cell division and a constitutively expressed, major outer membrane ..
  61. Walton T, Sandoval C, Fowler C, Pardi A, Sousa M. The cavity-chaperone Skp protects its substrate from aggregation but allows independent folding of substrate domains. Proc Natl Acad Sci U S A. 2009;106:1772-7 pubmed publisher
    ..The 17-kDa protein (Skp) is a periplasmic chaperone that assists the folding and insertion of many OMPs, including OmpA, a model OMP with a membrane embedded beta-barrel domain and a periplasmic alphabeta domain...
  62. Emory S, Bouvet P, Belasco J. A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coli. Genes Dev. 1992;6:135-48 pubmed
    The 5'-untranslated region of the long-lived Escherichia coli ompA transcript functions as an mRNA stabilizer capable of prolonging the lifetime in E. coli of a number of heterologous messages to which it is fused...
  63. Zoonens M, Catoire L, Giusti F, Popot J. NMR study of a membrane protein in detergent-free aqueous solution. Proc Natl Acad Sci U S A. 2005;102:8893-8 pubmed
    ..The potential of this approach to MP studies by solution NMR is discussed. ..
  64. Cierpicki T, Liang B, Tamm L, Bushweller J. Increasing the accuracy of solution NMR structures of membrane proteins by application of residual dipolar couplings. High-resolution structure of outer membrane protein A. J Am Chem Soc. 2006;128:6947-51 pubmed
    ..Here we demonstrate that significant improvement in the structure accuracy of the membrane protein OmpA can be achieved by refinement with residual dipolar couplings (RDCs)...
  65. Henning U, Sonntag I, Hindennach I. Mutants (ompA) affecting a major outer membrane protein of Escherichia coli K12. Eur J Biochem. 1978;92:491-8 pubmed
    ..Forty of the mutants were analyzed genetically and all were found to map at or near ompA, the structural gene for protein II...
  66. Cho S, Szewczyk J, Pesavento C, Zietek M, Banzhaf M, Roszczenko P, et al. Detecting envelope stress by monitoring β-barrel assembly. Cell. 2014;159:1652-64 pubmed publisher
    ..In growing cells, BamA continuously funnels RcsF through the β-barrel OmpA, displaying RcsF on the cell surface...
  67. Xian M, Fuerst M, Shabalin Y, Reusch R. Sorting signal of Escherichia coli OmpA is modified by oligo-(R)-3-hydroxybutyrate. Biochim Biophys Acta. 2007;1768:2660-6 pubmed
    Escherichia coli outer membrane protein A (OmpA) is a well-established model for the study of membrane assembly...
  68. Khalid S, Bond P, Carpenter T, Sansom M. OmpA: gating and dynamics via molecular dynamics simulations. Biochim Biophys Acta. 2008;1778:1871-80 pubmed
    ..The outer membrane protein, OmpA from Escherichia coli has been studied extensively in different environments both experimentally and ..
  69. Datta D, Vaidehi N, Floriano W, Kim K, Prasadarao N, Goddard W. Interaction of E. coli outer-membrane protein A with sugars on the receptors of the brain microvascular endothelial cells. Proteins. 2003;50:213-21 pubmed
    ..Experimental studies have shown that outer-membrane protein A (OmpA) of E...
  70. Strocchi M, Ferrer M, Timmis K, Golyshin P. Low temperature-induced systems failure in Escherichia coli: insights from rescue by cold-adapted chaperones. Proteomics. 2006;6:193-206 pubmed
    ..coli cells at low temperatures. ..
  71. Lilly A, Crane J, Randall L. Export chaperone SecB uses one surface of interaction for diverse unfolded polypeptide ligands. Protein Sci. 2009;18:1860-8 pubmed publisher
    ..We examined SecB in complex with the unfolded precursor form of outer membrane protein OmpA as well as with a truncated version of OmpA that includes the transmembrane domain and lacks both the signal ..
  72. Ramakrishnan M, Pocanschi C, Kleinschmidt J, Marsh D. Association of spin-labeled lipids with beta-barrel proteins from the outer membrane of Escherichia coli. Biochemistry. 2004;43:11630-6 pubmed
    ..The outer membrane protein OmpA and the ferrichrome-iron receptor FhuA from the outer membrane of Escherichia coli were reconstituted in bilayers ..
  73. Movva N, Nakamura K, Inouye M. Gene structure of the OmpA protein, a major surface protein of Escherichia coli required for cell-cell interaction. J Mol Biol. 1980;143:317-28 pubmed
  74. Arutyunov D, Arenson B, Manchak J, Frost L. F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation. J Bacteriol. 2010;192:1730-4 pubmed publisher
    ..Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH. ..
  75. Arora A, Rinehart D, Szabo G, Tamm L. Refolded outer membrane protein A of Escherichia coli forms ion channels with two conductance states in planar lipid bilayers. J Biol Chem. 2000;275:1594-600 pubmed
    Outer membrane protein A (OmpA), a major structural protein of the outer membrane of Escherichia coli, consists of an N-terminal 8-stranded beta-barrel transmembrane domain and a C-terminal periplasmic domain...
  76. Shin S, Lu G, Cai M, Kim K. Escherichia coli outer membrane protein A adheres to human brain microvascular endothelial cells. Biochem Biophys Res Commun. 2005;330:1199-204 pubmed
    ..The outer membrane protein A (OmpA) assembles a beta-barrel structure having four surface-exposed loops in E. coli outer membrane...
  77. Sugawara E, Nikaido H. OmpA protein of Escherichia coli outer membrane occurs in open and closed channel forms. J Biol Chem. 1994;269:17981-7 pubmed
    b>OmpA protein of Escherichia coli outer membrane can produce diffusion channels when reconstituted into proteoliposomes (Sugawara, E., and Nikaido, H. (1992) J. Biol. Chem. 267, 2507-2511)...
  78. Sugawara E, Nikaido H. Pore-forming activity of OmpA protein of Escherichia coli. J Biol Chem. 1992;267:2507-11 pubmed
    Escherichia coli outer membrane protein OmpA was purified to homogeneity, as a monomer, from a K12 derivative deficient in both OmpF and OmpC porins...
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