nusB

Summary

Gene Symbol: nusB
Description: transcription antitermination protein
Alias: ECK0410, JW0406, groNB, ssaD, ssyB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Torrents E, Grinberg I, Gorovitz Harris B, Lundström H, Borovok I, Aharonowitz Y, et al. NrdR controls differential expression of the Escherichia coli ribonucleotide reductase genes. J Bacteriol. 2007;189:5012-21 pubmed
    ..The model assumes that differences in the positions of the NrdR binding sites, and in the sequences of the motifs themselves, determine the extent to which NrdR represses the transcription of each RNR operon. ..
  2. Greive S, Lins A, von Hippel P. Assembly of an RNA-protein complex. Binding of NusB and NusE (S10) proteins to boxA RNA nucleates the formation of the antitermination complex involved in controlling rRNA transcription in Escherichia coli. J Biol Chem. 2005;280:36397-408 pubmed
    ..methods have been used to measure the equilibrium parameters that control the formation of the core subcomplex of NusB and NusE proteins and boxA RNA...
  3. Quan S, Zhang N, French S, Squires C. Transcriptional polarity in rRNA operons of Escherichia coli nusA and nusB mutant strains. J Bacteriol. 2005;187:1632-8 pubmed
    ..This modification requires nucleotide signals in the RNA and specific transcription factors, such as NusA and NusB. Transcription of rrn operons in strains lacking the ability to produce either NusA or NusB was examined by ..
  4. Torres M, Balada J, Zellars M, Squires C, Squires C. In vivo effect of NusB and NusG on rRNA transcription antitermination. J Bacteriol. 2004;186:1304-10 pubmed
    ..We have therefore analyzed the in vivo role of NusB and NusG in rRNA transcription antitermination and have established that both are essential for it...
  5. Lüttgen H, Robelek R, Mühlberger R, Diercks T, Schuster S, KOHLER P, et al. Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli. J Mol Biol. 2002;316:875-85 pubmed
    A recombinant heterodimeric NusB/NusE protein complex of Escherichia coli was expressed under the control of a synthetic mini operon...
  6. Altieri A, Mazzulla M, Horita D, Coats R, Wingfield P, Das A, et al. The structure of the transcriptional antiterminator NusB from Escherichia coli. Nat Struct Biol. 2000;7:470-4 pubmed
    We have determined the solution structure of NusB, a transcription antitermination protein from Escherichia coli. The structure reveals a novel, all alpha-helical protein fold...
  7. Court D, Patterson T, Baker T, Costantino N, Mao X, Friedman D. Structural and functional analyses of the transcription-translation proteins NusB and NusE. J Bacteriol. 1995;177:2589-91 pubmed
    The NusB and NusE (ribosomal protein S10) proteins function in transcription and translation...
  8. Nodwell J, Greenblatt J. Recognition of boxA antiterminator RNA by the E. coli antitermination factors NusB and ribosomal protein S10. Cell. 1993;72:261-8 pubmed
    ..We show that a complex of the transcription antitermination factors NusB and ribosomal protein S10 interacts specifically with boxA RNA...
  9. Barik S, Das A. An analysis of the role of host factors in transcription antitermination in vitro by the Q protein of coliphage lambda. Mol Gen Genet. 1990;222:152-6 pubmed
    ..based on Q-dependent synthesis of galactokinase from a pR'-tR'-galK template, we showed that mutations in the host nusB and nusE genes do not affect Q activity...

More Information

Publications43

  1. Carlomagno M, Nappo A. The antiterminator NusB enhances termination at a sub-optimal Rho site. J Mol Biol. 2001;309:19-28 pubmed
    Interactions between the antiterminator NusB and boxA elements in the nut sites are necessary to ensure lambda N-mediated processive antitermination...
  2. Strauch M, Friedman D. Identification of the nusB gene product of Escherichia coli. Mol Gen Genet. 1981;182:498-501 pubmed
    Escherichia coli nusB mutants fail to support the activity of a phage lambda gene product, pN, which regulates phage gene expression by influencing transcription termination...
  3. Swindle J, Ajioka J, Georgopoulos C. Identification of the E. coli groNB(nusB) gene product. Mol Gen Genet. 1981;182:409-13 pubmed
    The E. coli groNB(nusB) gene product has been previously shown to be necessary for bacteriophage lambda N protein function. The product of the groNB gene has been identified on SDS polyacrylamide gels after infection of UV-irradiated E...
  4. Bubunenko M, Court D, Al Refaii A, Saxena S, Korepanov A, Friedman D, et al. Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli. Mol Microbiol. 2013;87:382-93 pubmed publisher
    Escherichia coli NusA and NusB proteins bind specific sites, such as those in the leader and spacer sequences that flank the 16S region of the ribosomal RNA transcript, forming a complex with RNA polymerase that suppresses Rho-dependent ..
  5. Friedman D, Baumann M, Baron L. Cooperative effects of bacterial mutations affecting lambda N gene expression. I. Isolation and characterization of a nusB mutant. Virology. 1976;73:119-27 pubmed
  6. Burmann B, Luo X, Rosch P, Wahl M, Gottesman M. Fine tuning of the E. coli NusB:NusE complex affinity to BoxA RNA is required for processive antitermination. Nucleic Acids Res. 2010;38:314-26 pubmed publisher
    ..Interaction of E. coli NusB:NusE heterodimer with the single stranded BoxA motif of lambdanutL or lambdanutR RNA is crucial for this reaction...
  7. Huenges M, Rolz C, Gschwind R, Peteranderl R, Berglechner F, Richter G, et al. Solution structure of the antitermination protein NusB of Escherichia coli: a novel all-helical fold for an RNA-binding protein. EMBO J. 1998;17:4092-100 pubmed
    The NusB protein of Escherichia coli is involved in the regulation of rRNA biosynthesis by transcriptional antitermination. In cooperation with several other proteins, it binds to a dodecamer motif designated rrn boxA on the nascent rRNA...
  8. Miyashita T, Kano Y, Kuroki K, Ishii S, Imamoto F. In vivo evidence for nusA and nusB gene function in general transcription of the Escherichia coli genome. Biken J. 1982;25:121-30 pubmed
    The requirements for nusA and nusB gene products for the effective transcription of E. coli genes, including the trp operon, were demonstrated by analysis of RNA transcripts produced in nusA, nusB and nusAnusB mutants...
  9. Ward D, Gottesman M. The nus mutations affect transcription termination in Escherichia coli. Nature. 1981;292:212-5 pubmed
    ..The rho mutation HDF026 displays almost identical properties. The observations suggest that the nusA and nusB gene products may act as termination factors analogous to rho protein.
  10. Oliver D. Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli. J Bacteriol. 1985;161:285-91 pubmed
    ..The five remaining loci (ssaD, ssaE, ssaF, ssaG, and ssaH) have been mapped by P1 transduction and appear to define new genes in E. coli...
  11. Mason S, Li J, Greenblatt J. Direct interaction between two Escherichia coli transcription antitermination factors, NusB and ribosomal protein S10. J Mol Biol. 1992;223:55-66 pubmed
    The Escherichia coli proteins NusB and ribosomal protein S10 are important for transcription antitermination by the bacteriophage lambda N protein...
  12. Das R, Loss S, Li J, Waugh D, Tarasov S, Wingfield P, et al. Structural biophysics of the NusB:NusE antitermination complex. J Mol Biol. 2008;376:705-20 pubmed publisher
    ..As part of a process known as antitermination, two of these host factors, NusB and NusE, bind to form a heterodimer, which interacts with a specific boxA site on the RNA...
  13. Kuroki K, Ishii S, Kano Y, Miyashita T, Nishi K, Imamoto F. Involvement of the nusB gene products in transcription of Escherichia coli tryptophan operon in vitro. Mol Gen Genet. 1982;185:369-71 pubmed
    ..trp operon was studied using crude protein extracts (S-100) from Escherichia coli strains carrying the nusA or nusB mutation or both...
  14. Berglechner F, Richter G, Fischer M, Bacher A, Gschwind R, Huenges M, et al. Studies on the NusB protein of Escherichia coli--expression and determination of secondary-structure elements by multinuclear NMR spectroscopy. Eur J Biochem. 1997;248:338-46 pubmed
    The product of the nusB gene of Escherichia coli modulates the efficiency of transcription termination at nut (N utilization) sites of various bacterial and bacteriophage lambda genes...
  15. Carlomagno M, Nappo A. NusA modulates intragenic termination by different pathways. Gene. 2003;308:115-28 pubmed
    ..By contrast, GTTE1 is a suboptimal terminator, featuring a boxA element and requiring a functional NusB to terminate efficiently in vivo...
  16. Das A. How the phage lambda N gene product suppresses transcription termination: communication of RNA polymerase with regulatory proteins mediated by signals in nascent RNA. J Bacteriol. 1992;174:6711-6 pubmed
  17. Neely M, Friedman D. N-mediated transcription antitermination in lambdoid phage H-19B is characterized by alternative NUT RNA structures and a reduced requirement for host factors. Mol Microbiol. 2000;38:1074-85 pubmed
    ..H-19B N requires NusA, but not two other host factors required by lambda N, NusB and ribosomal protein S10. The H-19B NUT site BOXA is not required, whereas the BOXB is required for N action...
  18. Gardel C, Benson S, Hunt J, Michaelis S, Beckwith J. secD, a new gene involved in protein export in Escherichia coli. J Bacteriol. 1987;169:1286-90 pubmed
    ..coli chromosome, but separable from another gene proposed to be involved in export, ssaD, which maps nearby. A plasmid carrying secD+ was identified and used to show that the mutations are recessive...
  19. Altieri A, Mazzulla M, Zhou H, Costantino N, Court D, Byrd R. Sequential assignments and secondary structure of the RNA-binding transcriptional regulator NusB. FEBS Lett. 1997;415:221-6 pubmed
    The NusB protein is involved in transcriptional regulation in bacteriophage lambda...
  20. Rajapandi T, Oliver D. ssaD1, a suppressor of secA51(Ts) that renders growth of Escherichia coli cold sensitive, is an early amber mutation in the transcription factor gene nusB. J Bacteriol. 1994;176:4444-7 pubmed
    ..suppressor of the secA51(Ts) mutation that renders growth of Escherichia coli cold sensitive, was used to show that ssaD corresponds to nusB, a gene known to be important in transcription antitermination...
  21. Zellars M, Squires C. Antiterminator-dependent modulation of transcription elongation rates by NusB and NusG. Mol Microbiol. 1999;32:1296-304 pubmed
    ..Because several Nus factors have been implicated in rrn antitermination, we have examined the role of NusB, NusE and NusG in controlling the rate of rrn boxA-mediated transcript elongation...
  22. Sharrock R, Gourse R, Nomura M. Defective antitermination of rRNA transcription and derepression of rRNA and tRNA synthesis in the nusB5 mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1985;82:5275-9 pubmed
    ..6; that is, in the absence of the functional nusB gene product, premature transcription termination takes place within rRNA operons...
  23. Maekawa T, Nagase T, Imamoto F, Ishii S. Purification of the NusB gene product of Escherichia coli K12. Mol Gen Genet. 1985;200:14-20 pubmed
    The nucleotide sequence of the entire nusB gene of Escherichia coli has recently been determined and the amino acid sequence of its product deduced (Ishii et al. 1984; Swindle et al. 1984)...
  24. Ishii S, Hatada E, Maekawa T, Imamoto F. Molecular cloning and nucleotide sequencing of the nusB gene of E. coli. Nucleic Acids Res. 1984;12:4987-95 pubmed
    The nusB gene of E. coli has been cloned in plasmid pBR322. Using genetic complementation as an assay for the gene, its location in subclones was analyzed, and the nucleotide sequence of this gene and its flanking regions was determined...
  25. Taura T, Ueguchi C, Shiba K, Ito K. Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation. Mol Gen Genet. 1992;234:429-32 pubmed
    The Escherichia coli gene ssyB was cloned and sequenced...
  26. Imamoto F, Nakamura Y. Escherichia coli proteins involved in regulation of transcription termination: function, structure, and expression of the nusA and nusB genes. Adv Biophys. 1986;21:175-92 pubmed
  27. Sullivan S, Ward D, Gottesman M. Effect of Escherichia coli nusG function on lambda N-mediated transcription antitermination. J Bacteriol. 1992;174:1339-44 pubmed
    ..These results suggest that NusG may act as a component of the N antitermination complex. In addition, the data imply a role for NusG in Rho-dependent termination. ..
  28. Georgopoulos C, Swindle J, Keppel F, Ballivet M, Bisig R, Eisen H. Studies on the E. coli groNB (nusB) gene which affects bacteriophage lambda N gene function. Mol Gen Genet. 1980;179:55-61 pubmed
    Escherichia coli mutants, called groNB, which block the growth of bacteriophage lambda at the level of action of the gene N product, have been isolated as survivors at 42 degrees C of bacteria carrying a) the defective prophage lambda ..
  29. Arnvig K, Zeng S, Quan S, Papageorge A, Zhang N, Villapakkam A, et al. Evolutionary comparison of ribosomal operon antitermination function. J Bacteriol. 2008;190:7251-7 pubmed publisher
    ..coli, NusB. We monitored antitermination activity by assessing the ability of RNA polymerase to read through a factor-..
  30. Swindle J, Ajioka J, Dawson D, Myers R, Carroll D, Georgopoulos C. The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene. Nucleic Acids Res. 1984;12:4977-85 pubmed
    The nusB (groNB) gene product of Escherichia coli plays a pivotal role in allowing bacteriophage lambda N protein to function as an antiterminator of mRNA transcription and in modulating host gene expression...
  31. Mühlberger R, Robelek R, Eisenreich W, Ettenhuber C, Sinner E, Kessler H, et al. RNA DNA discrimination by the antitermination protein NusB. J Mol Biol. 2003;327:973-83 pubmed
    The regulation of ribosomal RNA biosynthesis in Escherichia coli by antitermination requires binding of NusB protein to a dodecamer sequence designated boxA on the nascent RNA...
  32. Schneider D, Gourse R. Changes in Escherichia coli rRNA promoter activity correlate with changes in initiating nucleoside triphosphate and guanosine 5' diphosphate 3'-diphosphate concentrations after induction of feedback control of ribosome synthesis. J Bacteriol. 2003;185:6185-91 pubmed
    ..In contrast, we observed no change in the level of DNA supercoiling under the feedback conditions examined. ..
  33. Swindle J, Zylicz M, Georgopoulos C, Li J, Greenblatt J. Purification and properties of the NusB protein of Escherichia coli. J Biol Chem. 1988;263:10229-35 pubmed
    Mutations in the nusB gene of Escherichia coli block transcriptional antitermination mediated by the N gene protein of bacteriophage lambda. We describe here two methods of overproducing the NusB protein in E...
  34. Bubunenko M, Baker T, Court D. Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli. J Bacteriol. 2007;189:2844-53 pubmed
    ..Interestingly, although most 30S ribosomal proteins were essential, the knockouts of six ribosomal protein genes, rpsF (S6), rpsI (S9), rpsM (S13), rpsO (S15), rpsQ (S17), and rpsT (S20), were viable. ..