nusA

Summary

Gene Symbol: nusA
Description: transcription termination/antitermination L factor
Alias: ECK3158, JW3138
Species: Escherichia coli str. K-12 substr. MG1655
Products:     nusA

Top Publications

  1. Bonin I, Mühlberger R, Bourenkov G, Huber R, Bacher A, Richter G, et al. Structural basis for the interaction of Escherichia coli NusA with protein N of phage lambda. Proc Natl Acad Sci U S A. 2004;101:13762-7 pubmed
    The C terminus of transcription factor NusA from Escherichia coli comprises two repeat units, which bind during antitermination to protein N from phage lambda...
  2. Cohen S, Walker G. The transcription elongation factor NusA is required for stress-induced mutagenesis in Escherichia coli. Curr Biol. 2010;20:80-5 pubmed publisher
    ..Loss of DinB (DNA pol IV) function results in a severe reduction of Lac(+) revertants. We previously reported that NusA, an essential component of elongating RNA polymerases, interacts with DinB...
  3. Farnham P, Greenblatt J, Platt T. Effects of NusA protein on transcription termination in the tryptophan operon of Escherichia coli. Cell. 1982;29:945-51 pubmed
    ..Increased specificity and sensitivity may also be provided by the NusA protein, which causes RNA polymerase to pause at trp t and at the 1:2 stem of the trp attenuator...
  4. Cardinale C, Washburn R, Tadigotla V, Brown L, Gottesman M, Nudler E. Termination factor Rho and its cofactors NusA and NusG silence foreign DNA in E. coli. Science. 2008;320:935-8 pubmed publisher
    ..Although rho remains essential, such reduced-genome bacteria no longer require Rho cofactors NusA and NusG. Deletion of the cryptic rac prophage in wild-type E...
  5. Luo X, Hsiao H, Bubunenko M, Weber G, Court D, Gottesman M, et al. Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex. Mol Cell. 2008;32:791-802 pubmed publisher
    ..These data demonstrate that S10 and NusB together form a BoxA-binding module, that NusB facilitates entry of S10 into the transcription machinery, and that S10 represents a central hub in processive antitermination. ..
  6. Linn T, Greenblatt J. The NusA and NusG proteins of Escherichia coli increase the in vitro readthrough frequency of a transcriptional attenuator preceding the gene for the beta subunit of RNA polymerase. J Biol Chem. 1992;267:1449-54 pubmed
    ..Both NusA and NusG increase the frequency of transcriptional readthrough at the attenuator whereas NusB, S10, and Rho had no ..
  7. Gill S, Weitzel S, von Hippel P. Escherichia coli sigma 70 and NusA proteins. I. Binding interactions with core RNA polymerase in solution and within the transcription complex. J Mol Biol. 1991;220:307-24 pubmed
    This paper describes the binding interactions of Escherichia coli transcription factors sigma 70 and NusA with core RNA polymerase, both free in solution and as a part of the functional transcription complex...
  8. Eisenmann A, Schwarz S, Prasch S, Schweimer K, Rosch P. The E. coli NusA carboxy-terminal domains are structurally similar and show specific RNAP- and lambdaN interaction. Protein Sci. 2005;14:2018-29 pubmed
    The carboxy-terminal domain of the transcription factor Escherichia coli NusA, NusACTD, interacts with the protein N of bacteriophage lambda, lambdaN, and the carboxyl terminus of the E. coli RNA polymerase alpha subunit, alphaCTD...
  9. Cohen S, Lewis C, Mooney R, Kohanski M, Collins J, Landick R, et al. Roles for the transcription elongation factor NusA in both DNA repair and damage tolerance pathways in Escherichia coli. Proc Natl Acad Sci U S A. 2010;107:15517-22 pubmed publisher
    We report observations suggesting that the transcription elongation factor NusA promotes a previously unrecognized class of transcription-coupled repair (TCR) in addition to its previously proposed role in recruiting translesion synthesis ..

More Information

Publications75

  1. Greenblatt J, Li J, Condon C, Squires C. Ribosomal RNA antitermination in vitro: requirement for Nus factors and one or more unidentified cellular components. Proc Natl Acad Sci U S A. 1993;90:970-4 pubmed
    ..a promoter-antiterminator-terminator arrangement, RNA polymerase, termination factor Rho, antitermination factors NusA, NusB, NusE, and NusG, and a cellular extract depleted of NusB...
  2. Liu K, Hanna M. NusA contacts nascent RNA in Escherichia coli transcription complexes. J Mol Biol. 1995;247:547-58 pubmed
    We have examined the interaction between NusA and the nascent RNA in Escherichia coli transcription complexes on four different templates. Photocrosslinking CTP and UTP analogs were incorporated internally and at the 3' end of the RNA...
  3. Prasch S, Jurk M, Washburn R, Gottesman M, Wohrl B, Rosch P. RNA-binding specificity of E. coli NusA. Nucleic Acids Res. 2009;37:4736-42 pubmed publisher
    ..The complex includes E. coli proteins NusA, NusB, NusG and NusE, and the lambda N protein...
  4. Carlomagno M, Nappo A. NusA modulates intragenic termination by different pathways. Gene. 2003;308:115-28 pubmed
    ..We analyzed the function of NusA in intragenic termination, making use of two tandem terminators located within the hisG cistron, GTTE1 and GTTE2...
  5. Mah T, Li J, Davidson A, Greenblatt J. Functional importance of regions in Escherichia coli elongation factor NusA that interact with RNA polymerase, the bacteriophage lambda N protein and RNA. Mol Microbiol. 1999;34:523-37 pubmed
    The association of the essential Escherichia coli protein NusA with RNA polymerase increases pausing and the efficiency of termination at intrinsic terminators...
  6. Prasch S, Schwarz S, Eisenmann A, Wohrl B, Schweimer K, Rosch P. Interaction of the intrinsically unstructured phage lambda N Protein with Escherichia coli NusA. Biochemistry. 2006;45:4542-9 pubmed
    ..coli host factor protein NusA, the viral nutBoxB-RNA, and RNA polymerase, respectively...
  7. Gill S, Yager T, von Hippel P. Escherichia coli sigma 70 and NusA proteins. II. Physical properties and self-association states. J Mol Biol. 1991;220:325-33 pubmed
    ..physical properties and potential for self-association of the Escherichia coli transcription factors, sigma 70 and NusA. We show, by a combination of chemical crosslinking, equilibrium and velocity sedimentation, quasi-elastic light ..
  8. Schmidt M, Chamberlin M. nusA protein of Escherichia coli is an efficient transcription termination factor for certain terminator sites. J Mol Biol. 1987;195:809-18 pubmed
    ..Termination efficiency at both of these sites is enhanced by the E. coli nusA protein, giving final efficiencies of termination in vitro comparable to those estimated in vivo...
  9. Mah T, Kuznedelov K, Mushegian A, Severinov K, Greenblatt J. The alpha subunit of E. coli RNA polymerase activates RNA binding by NusA. Genes Dev. 2000;14:2664-75 pubmed
    The Escherichia coli NusA protein modulates pausing, termination, and antitermination by associating with the transcribing RNA polymerase core enzyme...
  10. Zheng C, Friedman D. Reduced Rho-dependent transcription termination permits NusA-independent growth of Escherichia coli. Proc Natl Acad Sci U S A. 1994;91:7543-7 pubmed
    b>NusA and Rho are essential Escherichia coli proteins that influence transcription elongation and termination. We show that an E...
  11. Nakamura Y, Mizusawa S, Tsugawa A, Imai M. Conditionally lethal nusAts mutation of Escherichia coli reduces transcription termination but does not affect antitermination of bacteriophage lambda. Mol Gen Genet. 1986;204:24-8 pubmed
    ..the Escherichia coli trp a attenuator was examined in the conditionally lethal mutant (nusAts11) defective in the NusA protein of E. coli...
  12. Regnier P, Grunberg Manago M. RNase III cleavages in non-coding leaders of Escherichia coli transcripts control mRNA stability and genetic expression. Biochimie. 1990;72:825-34 pubmed
    The primary transcripts of the rpsO-pnp, rnc-era-recO and metY-nusA-infB operons of E coli are each processed by RNase III, upstream of the first translated gene, in hair-pin structures formed by the 5' non-coding leader...
  13. Plumbridge J, Springer M. Organization of the Escherichia coli chromosome around the genes for translation initiation factor IF2 (infB) and a transcription termination factor (nusA). J Mol Biol. 1983;167:227-43 pubmed
    The genes infB, for translational initiation factor IF2, and nusA for a protein involved in transcription termination are carried on a 4.8 X 10(3) base-pair DNA fragment...
  14. Eisenmann A, Schwarz S, Rosch P, Schweimer K. Sequence-specific 1H, 13C, 15N resonance assignments and secondary structure of the carboxyterminal domain of the E. coli transcription factor NusA. J Biomol NMR. 2004;28:193-4 pubmed
  15. Gibson T, Thompson J, Heringa J. The KH domain occurs in a diverse set of RNA-binding proteins that include the antiterminator NusA and is probably involved in binding to nucleic acid. FEBS Lett. 1993;324:361-6 pubmed
    ..Profile searches have revealed new members of this family, including two, E. coli NusA and human GAP-associated p62 phosphoprotein, for which RNA-binding data exists...
  16. Zhang Y, Hanna M. NusA changes the conformation of Escherichia coli RNA polymerase at the binding site for the 3' end of the nascent RNA. J Bacteriol. 1994;176:1787-9 pubmed
    A conformational change in Escherichia coli RNA polymerase induced by NusA was detected by utilizing photocrosslinking...
  17. Ishii S, Kuroki K, Imamoto F. tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli. Proc Natl Acad Sci U S A. 1984;81:409-13 pubmed
    The promoter-proximal portion of the operon containing the Escherichia coli nusA gene has been cloned. Its nucleotide sequence shows that genes for tRNAMetf2 and a 15-kilodalton protein of unknown function precede the nusA protein gene...
  18. Ishihama A, Honda A, Nagasawa Fujimori H, Glass R, Maekawa T, Imamoto F. Multivalent regulation of the nusA operon of Escherichia coli. Mol Gen Genet. 1987;206:185-91 pubmed
    The rate of synthesis and intracellular content of the NusA protein, a transcription termination factor, were determined for wild-type and nusA and/or nusB mutants of Escherichia coli...
  19. Traviglia S, Datwyler S, Yan D, Ishihama A, Meares C. Targeted protein footprinting: where different transcription factors bind to RNA polymerase. Biochemistry. 1999;38:15774-8 pubmed
    ..We used targeted protein footprinting to compare the areas near which sigma(70), sigma(54), sigma(38), sigma(E), NusA, GreA, and omega bind to the protein subunits of Escherichia coli RNAP...
  20. Bylund G, Lövgren J, Wikström P. Characterization of mutations in the metY-nusA-infB operon that suppress the slow growth of a DeltarimM mutant. J Bacteriol. 2001;183:6095-106 pubmed
    ..Nine of these mutations were in the nusA gene, which is located upstream from rbfA in the metY-nusA-infB operon; three mutations deleted the transcriptional ..
  21. Sands J, Regnier P, Cummings H, Grunberg Manago M, Hershey J. The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping. Nucleic Acids Res. 1988;16:10803-16 pubmed
    ..Escherichia coli genome map and are transcribed clockwise as two operons: the metY operon, containing metY, P15A, nusA, infB; and about a kilobase further downstream, the rpsO and pnp operon...
  22. Greenblatt J, Li J, Adhya S, Friedman D, Baron L, Redfield B, et al. L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination. Proc Natl Acad Sci U S A. 1980;77:1991-4 pubmed
    ..coli nusA gene...
  23. Kurihara T, Nakamura Y. Cloning of the nusA gene of Escherichia coli. Mol Gen Genet. 1983;190:189-95 pubmed
    ..3 kilobase segment defined by SalI and BglII sites. When the nusA gene segment was recloned into lambda vector L512, the resulting transducing phages lambda nusA+-1 and lambda nusA+-..
  24. Neely M, Friedman D. N-mediated transcription antitermination in lambdoid phage H-19B is characterized by alternative NUT RNA structures and a reduced requirement for host factors. Mol Microbiol. 2000;38:1074-85 pubmed
    ..We report on N antitermination in H-19B, a lambdoid phage carrying shiga toxin 1 genes. H-19B N requires NusA, but not two other host factors required by lambda N, NusB and ribosomal protein S10...
  25. Quan S, Zhang N, French S, Squires C. Transcriptional polarity in rRNA operons of Escherichia coli nusA and nusB mutant strains. J Bacteriol. 2005;187:1632-8 pubmed
    ..This modification requires nucleotide signals in the RNA and specific transcription factors, such as NusA and NusB...
  26. Plumbridge J, Howe J, Springer M, Touati Schwartz D, Hershey J, Grunberg Manago M. Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli. Proc Natl Acad Sci U S A. 1982;79:5033-7 pubmed
    ..coli chromosome, inf B has been located at 68 min, very close to argG, nusA, rpsO, and pnp...
  27. Artsimovitch I, Landick R. Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals. Proc Natl Acad Sci U S A. 2000;97:7090-5 pubmed
    ..pauses differ in their responses to antisense oligonucleotides, pyrophosphate, GreA, and general elongation factors NusA and NusG...
  28. Greenblatt J, McLimont M, Hanly S. Termination of transcription by nusA gene protein of Escherichia coli. Nature. 1981;292:215-20 pubmed
    The nusA gene protein of Escherichia coli and N gene protein of bacteriophage lambda interact in vitro and cooperate in vivo to prevent transcription termination...
  29. Jin D, Gross C. Three rpoBC mutations that suppress the termination defects of rho mutants also affect the functions of nusA mutants. Mol Gen Genet. 1989;216:269-75 pubmed
    ..The correlation of these two phenotypes suggests that both phenotypes may arise from the same functional defect in RNA polymerase. ..
  30. Watnick R, Gottesman M. Escherichia coli NusA is required for efficient RNA binding by phage HK022 nun protein. Proc Natl Acad Sci U S A. 1998;95:1546-51 pubmed
    ..Binding to BOXB is inhibited by Zn2+ and stimulated by the Escherichia coli NusA protein. Deletion of the Nun C-terminal region enhances BOXB binding and makes it independent of Zn2+ and NusA...
  31. Plumbridge J, Dondon J, Nakamura Y, Grunberg Manago M. Effect of NusA protein on expression of the nusA,infB operon in E. coli. Nucleic Acids Res. 1985;13:3371-88 pubmed
    Protein and operon fusions between lacZ and various genes of the nusA,infB operon have been constructed on lambda bacteriophages and used to show that the operon is negatively regulated by the level of NusA protein...
  32. Gusarov I, Nudler E. Control of intrinsic transcription termination by N and NusA: the basic mechanisms. Cell. 2001;107:437-49 pubmed
    ..Escherichia coli NusA protein destabilizes these interactions and thus promotes hairpin folding and termination...
  33. Regnier P, Grunberg Manago M. Cleavage by RNase III in the transcripts of the met Y-nus-A-infB operon of Escherichia coli releases the tRNA and initiates the decay of the downstream mRNA. J Mol Biol. 1989;210:293-302 pubmed
    ..tRNA is the first gene of a complex polycistronic operon also encoding the transcription termination factor NusA and the translation initiation factor IF2...
  34. Bubunenko M, Baker T, Court D. Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli. J Bacteriol. 2007;189:2844-53 pubmed
    ..Interestingly, although most 30S ribosomal proteins were essential, the knockouts of six ribosomal protein genes, rpsF (S6), rpsI (S9), rpsM (S13), rpsO (S15), rpsQ (S17), and rpsT (S20), were viable. ..
  35. Craven M, Granston A, Schauer A, Zheng C, Gray T, Friedman D. Escherichia coli-Salmonella typhimurium hybrid nusA genes: identification of a short motif required for action of the lambda N transcription antitermination protein. J Bacteriol. 1994;176:1394-404 pubmed
    The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable...
  36. Nehrke K, Platt T. A quaternary transcription termination complex. Reciprocal stabilization by Rho factor and NusG protein. J Mol Biol. 1994;243:830-9 pubmed
    ..Common structural and functional features shared with complexes that carry out processive antitermination are discussed. ..
  37. Cohen S, Godoy V, Walker G. Transcriptional modulator NusA interacts with translesion DNA polymerases in Escherichia coli. J Bacteriol. 2009;191:665-72 pubmed publisher
    b>NusA, a modulator of RNA polymerase, interacts with the DNA polymerase DinB...
  38. Schmidt M, Chamberlin M. Amplification and isolation of Escherichia coli nusA protein and studies of its effects on in vitro RNA chain elongation. Biochemistry. 1984;23:197-203 pubmed
    The Escherichia coli nusA gene product is an RNA polymerase binding protein which has been implicated in a variety of cellular and viral termination and antitermination processes...
  39. Li K, Jiang T, Yu B, Wang L, Gao C, Ma C, et al. Escherichia coli transcription termination factor NusA: heat-induced oligomerization and chaperone activity. Sci Rep. 2013;3:2347 pubmed publisher
    Escherichia coli NusA, an essential component of the RNA polymerase elongation complex, is involved in transcriptional elongation, termination, anti-termination, cold shock and stress-induced mutagenesis...
  40. Nakamura Y, Mizusawa S. In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins. EMBO J. 1985;4:527-32 pubmed
    Previous work has shown that the Escherichia coli nusA gene codes for a protein which regulates transcription termination. The 16.0-kb EcoRI DNA fragment that includes the nusA gene, codes for at least eight bacterial proteins of mol. wts...
  41. Cohen S, Walker G. New discoveries linking transcription to DNA repair and damage tolerance pathways. Transcription. 2011;2:37-40 pubmed publisher
    In Escherichia coli, the transcription elongation factor NusA is associated with all elongating RNA polymerases where it functions in transcription termination and antitermination...
  42. Ito K, Nakamura Y. Localization of nusA-suppressing amino acid substitutions in the conserved regions of the beta' subunit of Escherichia coli RNA polymerase. Mol Gen Genet. 1996;251:699-706 pubmed
    ..in a plasmid-borne copy of rpoC, which encodes beta', as dominant suppressors of two temperature-sensitive nusA alleles...
  43. Liu K, Hanna M. NusA interferes with interactions between the nascent RNA and the C-terminal domain of the alpha subunit of RNA polymerase in Escherichia coli transcription complexes. Proc Natl Acad Sci U S A. 1995;92:5012-6 pubmed
    The effects of NusA on the RNA polymerase contacts made by nucleotides at internal positions in the nascent RNA in Escherichia coli transcription complexes were analyzed by using the photocrosslinking nucleotide analog 5-[(4-azidophenacyl)..
  44. Ishii S, Ihara M, Maekawa T, Nakamura Y, Uchida H, Imamoto F. The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli. Nucleic Acids Res. 1984;12:3333-42 pubmed
    The nucleotide sequence of the promoter-proximal portion of the nusA operon including the genes for tRNAMetf2, a 15 kilodalton protein and the initial portion of the nusA gene has been determined previously (1)...
  45. Wong T, Sosnick T, Pan T. Mechanistic insights on the folding of a large ribozyme during transcription. Biochemistry. 2005;44:7535-42 pubmed
    ..RNase P RNA during its transcription by the Escherichia coli RNA polymerase and the effect of the elongation factor NusA. We found that transcriptional pausing at a specific site induced by NusA significantly altered the folding pathway...
  46. Mishra S, Sen R. N protein from lambdoid phages transforms NusA into an antiterminator by modulating NusA-RNA polymerase flap domain interactions. Nucleic Acids Res. 2015;43:5744-58 pubmed publisher
    Interaction of the lambdoid phage N protein with the bacterial transcription elongation factor NusA is the key component in the process of transcription antitermination. A convex surface of E...
  47. Burns C, Richardson L, Richardson J. Combinatorial effects of NusA and NusG on transcription elongation and Rho-dependent termination in Escherichia coli. J Mol Biol. 1998;278:307-16 pubmed
    The transcription factors NusA and NusG from Escherichia coli are modulators of the RNA polymerase elongation reaction and Rho-dependent transcription termination...
  48. Barik S, Das A. An analysis of the role of host factors in transcription antitermination in vitro by the Q protein of coliphage lambda. Mol Gen Genet. 1990;222:152-6 pubmed
    ..A mutation in nusA (nusA1) only partially affects Q action at all temperatures tested...
  49. Jin D, Cashel M, Friedman D, Nakamura Y, Walter W, Gross C. Effects of rifampicin resistant rpoB mutations on antitermination and interaction with nusA in Escherichia coli. J Mol Biol. 1988;204:247-61 pubmed
    ..Because NusA is involved in antitermination and termination, we also investigated the interaction of NusA and RNA polymerase by ..
  50. Saito M, Tsugawa A, Egawa K, Nakamura Y. Revised sequence of the nusA gene of Escherichia coli and identification of nusA11 (ts) and nusA1 mutations which cause changes in a hydrophobic amino acid cluster. Mol Gen Genet. 1986;205:380-2 pubmed
    The mutant nusA DNAs (nusA11 and nusA1) were sequenced. Single base substitutions caused by these mutations were found in the coding region of nusA. The nusA11 mutation, which is conditionally lethal, substituted Thr for the 181st Ala...
  51. Ward D, Gottesman M. The nus mutations affect transcription termination in Escherichia coli. Nature. 1981;292:212-5 pubmed
    ..The rho mutation HDF026 displays almost identical properties. The observations suggest that the nusA and nusB gene products may act as termination factors analogous to rho protein.
  52. Sha Y, Lindahl L, Zengel J. Role of NusA in L4-mediated attenuation control of the S10 r-protein operon of Escherichia coli. J Mol Biol. 1995;245:474-85 pubmed
    ..The in vivo effect can be reproduced in a purified transcription system but requires an additional factor, NusA. Our earlier in vitro studies showed that NusA is required for RNA polymerase pausing at the termination site; such ..
  53. Shankar S, Hatoum A, Roberts J. A transcription antiterminator constructs a NusA-dependent shield to the emerging transcript. Mol Cell. 2007;27:914-27 pubmed
    The universal bacterial transcription elongation factor NusA mediates elongation activities of RNA polymerase...
  54. Zhang Y, Hanna M. Expression and functional characterization of Escherichia coli NusA and lambda Q as glutathione S-transferase fusion proteins. Protein Expr Purif. 1995;6:625-31 pubmed
    The Escherichia coli transcription factor NusA and the bacteriophage lambda antiterminator Q proteins were expressed as inducible glutathione S-transferase (GST) fusion proteins...
  55. Miyashita T, Kano Y, Kuroki K, Ishii S, Imamoto F. In vivo evidence for nusA and nusB gene function in general transcription of the Escherichia coli genome. Biken J. 1982;25:121-30 pubmed
    The requirements for nusA and nusB gene products for the effective transcription of E. coli genes, including the trp operon, were demonstrated by analysis of RNA transcripts produced in nusA, nusB and nusAnusB mutants...
  56. Liu K, Zhang Y, Severinov K, Das A, Hanna M. Role of Escherichia coli RNA polymerase alpha subunit in modulation of pausing, termination and anti-termination by the transcription elongation factor NusA. EMBO J. 1996;15:150-61 pubmed
    ..that alpha also plays a role in transcription elongation, and this involves a direct interaction between alpha and NusA factor...
  57. Mooney R, Davis S, Peters J, Rowland J, Ansari A, Landick R. Regulator trafficking on bacterial transcription units in vivo. Mol Cell. 2009;33:97-108 pubmed publisher
    The trafficking patterns of the bacterial regulators of transcript elongation sigma(70), rho, NusA, and NusG on genes in vivo and the explanation for promoter-proximal peaks of RNA polymerase (RNAP) are unknown. Genome-wide, E...
  58. Nakamura Y, Plumbridge J, Dondon J, Grunberg Manago M. Evidence for autoregulation of the nusA-infB operon of Escherichia coli. Gene. 1985;36:189-93 pubmed
    Analysis of three different nusA mutant strains suggests that the expression of the nusA-infB operon of Escherichia coli is regulated autogenously by the nusA gene product, a protein known to mediate transcription termination and ..
  59. Van Gilst M, von Hippel P. Assembly of the N-dependent antitermination complex of phage lambda: NusA and RNA bind independently to different unfolded domains of the N protein. J Mol Biol. 1997;274:160-73 pubmed
    ..the N-boxB subassembly to the transcription complex is further stabilized by interaction with the Escherichia coli NusA protein...
  60. Ito K, Egawa K, Nakamura Y. Genetic interaction between the beta' subunit of RNA polymerase and the arginine-rich domain of Escherichia coli nusA protein. J Bacteriol. 1991;173:1492-501 pubmed
    ..During these studies, we found three -1 frameshift errors in the wild-type nusA sequence; the correct sequence was confirmed by the peptide sequence and gene fusion analyses...
  61. Sullivan S, Ward D, Gottesman M. Effect of Escherichia coli nusG function on lambda N-mediated transcription antitermination. J Bacteriol. 1992;174:1339-44 pubmed
    ..b>NusA binds both N and RNA polymerase and may also interact with other Nus factors...
  62. Watt R, Wang J, Leong M, Kung H, Cheah K, Liu D, et al. Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. Nucleic Acids Res. 2007;35:e37 pubmed
    ..Our results show that E. coli has an organized and dynamic proteome, and demonstrate that this approach is applicable for tagging and (co-) localizing CDSs on a genome-wide scale. ..
  63. Wade J, Struhl K. Association of RNA polymerase with transcribed regions in Escherichia coli. Proc Natl Acad Sci U S A. 2004;101:17777-82 pubmed
    ..the association of the beta-, alpha-, and sigma(70)-subunits of Escherichia coli RNA polymerase (RNAP) and the NusA elongation factor with transcribed regions in vivo by using chromatin immunoprecipitation...
  64. Saxena S, Gowrishankar J. Compromised factor-dependent transcription termination in a nusA mutant of Escherichia coli: spectrum of termination efficiencies generated by perturbations of Rho, NusG, NusA, and H-NS family proteins. J Bacteriol. 2011;193:3842-50 pubmed publisher
    The proteins NusA and NusG, which are essential for the viability of wild-type Escherichia coli, participate in various postinitiation steps of transcription including elongation, antitermination, and termination...
  65. Bubunenko M, Court D, Al Refaii A, Saxena S, Korepanov A, Friedman D, et al. Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli. Mol Microbiol. 2013;87:382-93 pubmed publisher
    Escherichia coli NusA and NusB proteins bind specific sites, such as those in the leader and spacer sequences that flank the 16S region of the ribosomal RNA transcript, forming a complex with RNA polymerase that suppresses Rho-dependent ..
  66. Craven M, Friedman D. Analysis of the Escherichia coli nusA10(Cs) allele: relating nucleotide changes to phenotypes. J Bacteriol. 1991;173:1485-91 pubmed
    The Escherichia coli nusA gene product, known to influence transcription elongation, is essential for both bacterial viability and growth of lambdoid phages...