Gene Symbol: nupC
Description: nucleoside (except guanosine) transporter
Alias: ECK2388, JW2389, cru
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Zhang Y, Craig J, Gallagher M. Location of the nupC gene on the physical map of Escherichia coli K-12. J Bacteriol. 1992;174:5758-9 pubmed
  2. Brun Y, Breton R, Lanouette P, Lapointe J. Precise mapping and comparison of two evolutionarily related regions of the Escherichia coli K-12 chromosome. Evolution of valU and lysT from an ancestral tRNA operon. J Mol Biol. 1990;214:825-43 pubmed the 52 minute region has permitted precise mapping of most of the 18 genes in this region with the order nupC-glk- less than (alaW beta-ala W alpha)-1 kb- less than gltX-0...
  3. Saier M, Eng B, Fard S, Garg J, Haggerty D, Hutchinson W, et al. Phylogenetic characterization of novel transport protein families revealed by genome analyses. Biochim Biophys Acta. 1999;1422:1-56 pubmed
    ..We tabulate the currently sequenced members of the 26 families analyzed, describe the properties of these families, and present partial multiple alignments, signature sequences and phylogenetic trees for them all. ..
  4. Patching S, Baldwin S, Baldwin A, Young J, Gallagher M, Henderson P, et al. The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands. Org Biomol Chem. 2005;3:462-70 pubmed
    ..tested as inhibitors of [U-(14)C]uridine uptake by the concentrative, H(+)-linked nucleoside transport proteins NupC and NupG from Escherichia coli...
  5. Komatsu Y, Tanaka K. A showdomycin-resistant mutant of Escherichia coli K-12 with altered nucleoside transport character. Biochim Biophys Acta. 1972;288:390-403 pubmed
  6. Buxton R, Hammer Jespersen K, Valentin Hansen P. A second purine nucleoside phosphorylase in Escherichia coli K-12. I. Xanthosine phosphorylase regulatory mutants isolated as secondary-site revertants of a deoD mutant. Mol Gen Genet. 1980;179:331-40 pubmed
  7. Munch Petersen A, Mygind B. Nucleoside transport systems in Escherichia coli K12: specificity and regulation. J Cell Physiol. 1976;89:551-9 pubmed
    ..Enzyme assays performed on whole cells of strains, able or unable to transport nucleosides, indicate that the nucleoside catabolizing enzymes are located inside the permeability barrier of the cell. ..
  8. Loewen S, Yao S, Slugoski M, Mohabir N, Turner R, Mackey J, et al. Transport of physiological nucleosides and anti-viral and anti-neoplastic nucleoside drugs by recombinant Escherichia coli nucleoside-H(+) cotransporter (NupC) produced in Xenopus laevis oocytes. Mol Membr Biol. 2004;21:1-10 pubmed
    ..CNT1, CNT2 and CNT3 belong to a gene family (CNT) that also includes the bacterial nucleoside transport protein NupC. Heterologous expression in Xenopus oocytes has established that CNT1-3 correspond functionally to the three major ..
  9. Mygind B, Munch Petersen -. Transport of pyrimidine nucleosides in cells of Escherichia coli K 12. Eur J Biochem. 1975;59:365-72 pubmed
    ..The transport system is repressed by growth on glucose, as is the synthesis of the enzymes. ..

More Information


  1. Komatsu Y. Adenosine uptake by isolated membrane vesicles from Escherichia coli K-12. Biochim Biophys Acta. 1973;330:206-21 pubmed
  2. Nørholm M, Dandanell G. Specificity and topology of the Escherichia coli xanthosine permease, a representative of the NHS subfamily of the major facilitator superfamily. J Bacteriol. 2001;183:4900-4 pubmed
    The specificity of XapB permease was compared with that of the known nucleoside transporters NupG and NupC. XapB-mediated xanthosine uptake is abolished by 2,4-dinitrophenol and exhibits saturation kinetics with an apparent K(m) of 136 ..
  3. Craig J, Zhang Y, Gallagher M. Cloning of the nupC gene of Escherichia coli encoding a nucleoside transport system, and identification of an adjacent insertion element, IS 186. Mol Microbiol. 1994;11:1159-68 pubmed
    ..In the present study we report the sequence of a gene encoding a second nucleoside transport system, nupC (in addition to nupG)...
  4. Munch Petersen A, Mygind B, Nicolaisen A, Pihl N. Nucleoside transport in cells and membrane vesicles from Escherichia coli K12. J Biol Chem. 1979;254:3730-7 pubmed
    ..This finding is consistent with a requirement for a specific transport process prior to the initial enzymatic attack on the incoming nucleoside. ..
  5. Komatsu Y, Tanaka K. Deoxycytidine uptake by isolated membrane vesicles from Escherichia coli K 12. Biochim Biophys Acta. 1973;311:496-506 pubmed