Gene Symbol: nsrR
Description: nitric oxide-sensitive repressor for NO regulon
Alias: ECK4174, JW4136, yjeB
Species: Escherichia coli str. K-12 substr. MG1655
Products:     nsrR

Top Publications

  1. Schwartz C, Giel J, Patschkowski T, Luther C, Ruzicka F, Beinert H, et al. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci U S A. 2001;98:14895-900 pubmed publisher
  2. Todd J, Wexler M, Sawers G, Yeoman K, Poole P, Johnston A. RirA, an iron-responsive regulator in the symbiotic bacterium Rhizobium leguminosarum. Microbiology. 2002;148:4059-71 pubmed
    ..Mutations in rirA reduced growth rates in Fe-replete and -depleted medium, but did not appear to affect symbiotic N(2) fixation. ..
  3. Beaumont H, Lens S, Reijnders W, Westerhoff H, van Spanning R. Expression of nitrite reductase in Nitrosomonas europaea involves NsrR, a novel nitrite-sensitive transcription repressor. Mol Microbiol. 2004;54:148-58 pubmed
    ..We identified a gene, nsrR, which encodes a novel nitrite (NO(2) (-))-sensitive transcription repressor that plays a pivotal role in the ..
  4. Rodionov D, Dubchak I, Arkin A, Alm E, Gelfand M. Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks. PLoS Comput Biol. 2005;1:e55 pubmed
    ..DNR, and NnrR; two-component systems NarXL and NarQP; NO-responsive activator NorR; and nitrite-sensitive repressor NsrR. Using comparative genomics approaches, we predict DNA-binding motifs for these transcriptional factors and ..
  5. Bodenmiller D, Spiro S. The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator. J Bacteriol. 2006;188:874-81 pubmed
    ..On the basis of similarity to the nitrite-responsive repressor NsrR from Nitrosomonas europaea, we propose that the yjeB gene of E. coli be renamed nsrR.
  6. Filenko N, Spiro S, Browning D, Squire D, Overton T, Cole J, et al. The NsrR regulon of Escherichia coli K-12 includes genes encoding the hybrid cluster protein and the periplasmic, respiratory nitrite reductase. J Bacteriol. 2007;189:4410-7 pubmed
    ..The regulatory protein NsrR (a member of the Rrf2 family of transcription factors) plays key roles in this stress response...
  7. Lin H, Bledsoe P, Stewart V. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen- responsive regulator Fnr in Escherichia coli K-12. J Bacteriol. 2007;189:7539-48 pubmed
    ..Recently, yeaR-yoaG operon transcription was shown to be regulated by the nitric oxide-responsive NsrR repressor (N. Filenko et al., J. Bacteriol. 189:4410-4417, 2007)...
  8. Chhabra S, Spiro S. Inefficient translation of nsrR constrains behaviour of the NsrR regulon in Escherichia coli. Microbiology. 2015;161:2029-38 pubmed publisher
    The NsrR protein of Escherichia coli is a transcriptional repressor that contains an [Fe-S] cluster that is the binding site for nitric oxide (NO)...
  9. Partridge J, Bodenmiller D, Humphrys M, Spiro S. NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motility. Mol Microbiol. 2009;73:680-94 pubmed publisher
    The Escherichia coli NsrR protein is a nitric oxide-sensitive repressor of transcription. The NsrR-binding site is predicted to comprise two copies of an 11 bp motif arranged as an inverted repeat with 1 bp spacing...

More Information


  1. Tucker N, Hicks M, Clarke T, Crack J, Chandra G, Le Brun N, et al. The transcriptional repressor protein NsrR senses nitric oxide directly via a [2Fe-2S] cluster. PLoS ONE. 2008;3:e3623 pubmed publisher
    The regulatory protein NsrR, a member of the Rrf2 family of transcription repressors, is specifically dedicated to sensing nitric oxide (NO) in a variety of pathogenic and non-pathogenic bacteria...
  2. Rankin L, Bodenmiller D, Partridge J, Nishino S, Spain J, Spiro S. Escherichia coli NsrR regulates a pathway for the oxidation of 3-nitrotyramine to 4-hydroxy-3-nitrophenylacetate. J Bacteriol. 2008;190:6170-7 pubmed publisher
    ..immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes...
  3. Efromovich S, Grainger D, Bodenmiller D, Spiro S. Genome-wide identification of binding sites for the nitric oxide-sensitive transcriptional regulator NsrR. Methods Enzymol. 2008;437:211-33 pubmed publisher
    b>NsrR is a nitric oxide-sensitive regulator of transcription. In Escherichia coli, NsrR is a repressor of the hmp gene encoding the flavohemoglobin that detoxifies nitric oxide...
  4. Wolfe S, Smith J. Nucleotide sequence and analysis of the purA gene encoding adenylosuccinate synthetase of Escherichia coli K12. J Biol Chem. 1988;263:19147-53 pubmed
    ..The purA control region did not resemble the control regions of the other known pur loci. ..
  5. Thompson K, Rhodius V, Gottesman S. SigmaE regulates and is regulated by a small RNA in Escherichia coli. J Bacteriol. 2007;189:4243-56 pubmed
    ..Based on the plasmid screens, NsrR, a repressor sensitive to nitric oxide, was also found to negatively regulate sigma(E)-dependent promoters in an ..
  6. Pullan S, Gidley M, Jones R, Barrett J, Stevanin T, Read R, et al. Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation. J Bacteriol. 2007;189:1845-55 pubmed
    ..Other global regulators apparently affected by NO were IscR, Fur, SoxR, NsrR, and NorR...
  7. Jain C. Identification and characterization of growth suppressors of Escherichia coli strains lacking phosphorolytic ribonucleases. J Bacteriol. 2009;191:5622-7 pubmed publisher
    ..The majority of suppressor mutations were found to lie within nsrR, which encodes a nitric oxide (NO)-sensitive transcriptional repressor...
  8. Cairrao F, Cruz A, Mori H, Arraiano C. Cold shock induction of RNase R and its role in the maturation of the quality control mediator SsrA/tmRNA. Mol Microbiol. 2003;50:1349-60 pubmed
    ..The wide biological significance of RNase R regarding adaptation to cold shock and its involvement in RNA surveillance, protein quality control and pathogenesis is discussed. ..
  9. Browning D, Lee D, Spiro S, Busby S. Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site. J Bacteriol. 2010;192:3824-8 pubmed publisher
    FNR-dependent activation of the Escherichia coli K-12 nrf promoter is downregulated by the nitric oxide-sensitive NsrR protein together with the nucleoid-associated protein IHF, which bind to overlapping targets adjacent to the DNA site ..
  10. Vinella D, Loiseau L, Ollagnier de Choudens S, Fontecave M, Barras F. In vivo [Fe-S] cluster acquisition by IscR and NsrR, two stress regulators in Escherichia coli. Mol Microbiol. 2013;87:493-508 pubmed publisher
    The multi-proteins Isc and Suf systems catalyse the biogenesis of [Fe-S] proteins. Here we investigate how NsrR and IscR, transcriptional regulators that sense NO and [Fe-S] homeostasis, acquire their [Fe-S] clusters under both normal and ..
  11. L vgren J, Wikstr m P. The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli. J Bacteriol. 2001;183:6957-60 pubmed publisher
    ..However, a DeltarlmB mutant did not show any ribosome assembly defects and was not outgrown by a wild-type strain even after 120 cell mass doublings. Thus, RlmB has no important role in ribosome assembly or function in E. coli...
  12. D Autréaux B, Tucker N, Spiro S, Dixon R. Characterization of the nitric oxide-reactive transcriptional activator NorR. Methods Enzymol. 2008;437:235-51 pubmed publisher
    ..It also details spectroscopic methods for analysis of the interaction of NO with the nonheme iron center and determination of the NO-binding affinity constant. ..
  13. Pullan S, Monk C, Lee L, Poole R. Microbial responses to nitric oxide and nitrosative stress: growth, "omic," and physiological methods. Methods Enzymol. 2008;437:499-519 pubmed publisher
    ..A key to the literature describing methods suitable for assessing toxicity to microbes of NO and reactive nitrogen species is given. ..