Gene Symbol: nrdE
Description: ribonucleoside-diphosphate reductase 2, alpha subunit
Alias: ECK2669, JW2650
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Jordan A, Pontis E, Atta M, Krook M, Gibert I, Barbe J, et al. A second class I ribonucleotide reductase in Enterobacteriaceae: characterization of the Salmonella typhimurium enzyme. Proc Natl Acad Sci U S A. 1994;91:12892-6 pubmed
    ..Both organisms contain two additional chromosomal genes, nrdE and nrdF, whose corresponding protein sequences show some homology to the products of the genes nrdA and nrdB...
  2. Vassinova N, Kozyrev D. A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli. Microbiology. 2000;146 Pt 12:3171-82 pubmed
    ..The regulation of the promoter activities by iron and the involvement of Fur in this regulation were shown. Employing FUR-SEL1 for cloning Fur-regulated loci from other enterobacteria is discussed. ..
  3. Gon S, Faulkner M, Beckwith J. In vivo requirement for glutaredoxins and thioredoxins in the reduction of the ribonucleotide reductases of Escherichia coli. Antioxid Redox Signal. 2006;8:735-42 pubmed
    ..These findings indicate a role for these enzymes either for NrdDG reactivation or some other essential anaerobic process. ..
  4. Cotruvo J, Stubbe J. NrdI, a flavodoxin involved in maintenance of the diferric-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase. Proc Natl Acad Sci U S A. 2008;105:14383-8 pubmed publisher
    ..The class Ib RNR, composed of NrdE (alpha) and NrdF (beta), is expressed under oxidative stress and iron-limited growth conditions...
  5. Martin J, Imlay J. The alternative aerobic ribonucleotide reductase of Escherichia coli, NrdEF, is a manganese-dependent enzyme that enables cell replication during periods of iron starvation. Mol Microbiol. 2011;80:319-34 pubmed publisher
    ..Thus NrdEF supports DNA replication when iron is unavailable to activate the housekeeping NrdAB enzyme. ..
  6. Kubo K, Craig N. Bacterial transposon Tn7 utilizes two different classes of target sites. J Bacteriol. 1990;172:2774-8 pubmed
    ..The target site choice was found to be determined by Tn7-encoded transposition genes. ..
  7. Jordan A, Aragall E, Gibert I, Barbe J. Promoter identification and expression analysis of Salmonella typhimurium and Escherichia coli nrdEF operons encoding one of two class I ribonucleotide reductases present in both bacteria. Mol Microbiol. 1996;19:777-90 pubmed
    ..The +1 position was 691 bp and 692 bp upstream of the translational start points of the nrdE genes of S. typhimurium and E. coli, respectively...
  8. Jordan A, Aslund F, Pontis E, Reichard P, Holmgren A. Characterization of Escherichia coli NrdH. A glutaredoxin-like protein with a thioredoxin-like activity profile. J Biol Chem. 1997;272:18044-50 pubmed
    ..We also show that NrdI, encoded by all nrdEF operons, has a stimulatory effect on ribonucleotide reduction. ..
  9. Monje Casas F, Jurado J, Prieto Alamo M, Holmgren A, Pueyo C. Expression analysis of the nrdHIEF operon from Escherichia coli. Conditions that trigger the transcript level in vivo. J Biol Chem. 2001;276:18031-7 pubmed
    ..We propose that E. coli might optimize the responses to different stimuli by co-evolving the expression levels for its multiple reductases and electron donors. ..

Scientific Experts

More Information


  1. Kolberg M, Strand K, Graff P, Andersson K. Structure, function, and mechanism of ribonucleotide reductases. Biochim Biophys Acta. 2004;1699:1-34 pubmed
  2. Justino M, Vicente J, Teixeira M, Saraiva L. New genes implicated in the protection of anaerobically grown Escherichia coli against nitric oxide. J Biol Chem. 2005;280:2636-43 pubmed
    ..coli gene transcription profile that includes the increase of the transcript level of genes encoding for detoxification enzymes, iron-sulfur cluster assembly systems, DNA-repairing enzymes, and stress response regulators. ..
  3. Torrents E, Grinberg I, Gorovitz Harris B, Lundström H, Borovok I, Aharonowitz Y, et al. NrdR controls differential expression of the Escherichia coli ribonucleotide reductase genes. J Bacteriol. 2007;189:5012-21 pubmed
    ..The model assumes that differences in the positions of the NrdR binding sites, and in the sequences of the motifs themselves, determine the extent to which NrdR represses the transcription of each RNR operon. ..
  4. Minnihan E, Seyedsayamdost M, Stubbe J. Use of 3-aminotyrosine to examine the pathway dependence of radical propagation in Escherichia coli ribonucleotide reductase. Biochemistry. 2009;48:12125-32 pubmed publisher
    ..2-0.3% of that observed at 731 and 730, and with (c), no NH(2)Y(*) was observed. These studies suggest the evolution of an optimized pathway of conserved Ys in the oxidation of C(439). ..