Gene Symbol: nrdB
Description: ribonucleotide-diphosphate reductase subunit beta
Species: Escherichia coli O157:H7 str. Sakai
Nordlund P, Sjoberg B, Eklund H. Three-dimensional structure of the free radical protein of ribonucleotide reductase. Nature. 1990;345:593-8 pubmed
..Tyrosine 122, which harbours the stable free radical necessary for the activity of ribonucleotide reductase, is buried inside the protein and is located 5 A from the closest iron atom. ..
Högbom M, Andersson M, Nordlund P. Crystal structures of oxidized dinuclear manganese centres in Mn-substituted class I ribonucleotide reductase from Escherichia coli: carboxylate shifts with implications for O2 activation and radical generation. J Biol Inorg Chem. 2001;6:315-23 pubmed
..Probable catalase activity was also observed during the oxidation with H2O2, indicating mechanistic similarities to the di-Mn catalases. ..
Nordlund P, Eklund H. Structure and function of the Escherichia coli ribonucleotide reductase protein R2. J Mol Biol. 1993;232:123-64 pubmed
Tong W, Burdi D, Riggs Gelasco P, Chen S, Edmondson D, Huynh B, et al
. Characterization of Y122F R2 of Escherichia coli ribonucleotide reductase by time-resolved physical biochemical methods and X-ray crystallography. Biochemistry. 1998;37:5840-8 pubmed
..Studies with two mutants, Y356F and double mutant Y356F and Y122F R2, are interpreted in terms of the possible role of Y356 in the putative electron transfer reaction between the R1 and R2 subunits of this RNR. ..
Logan D, deMaré F, Persson B, Slaby A, Sjoberg B, Nordlund P. Crystal structures of two self-hydroxylating ribonucleotide reductase protein R2 mutants: structural basis for the oxygen-insertion step of hydroxylation reactions catalyzed by diiron proteins. Biochemistry. 1998;37:10798-807 pubmed
..A critical role for residue Glu238 in directing the oxidative power of the reactive intermediate toward oxidation of Tyr122 is proposed. ..
Logan D, Su X, Aberg A, Regnstrom K, Hajdu J, Eklund H, et al
. Crystal structure of reduced protein R2 of ribonucleotide reductase: the structural basis for oxygen activation at a dinuclear iron site. Structure. 1996;4:1053-64 pubmed
..The Ser211--> Ala mutant displays a conformational change in the helix containing the mutation, explaining its altered reduction kinetics. ..
Salowe S, Stubbe J. Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase. J Bacteriol. 1986;165:363-6 pubmed
The nrdB gene, which encodes the B2 subunit of Escherichia coli ribonucleotide reductase (EC 22.214.171.124), was cloned into multicopy plasmid pSPS2...
Garriga X, Eliasson R, Torrents E, Jordan A, Barbe J, Gibert I, et al
. nrdD and nrdG genes are essential for strict anaerobic growth of Escherichia coli. Biochem Biophys Res Commun. 1996;229:189-92 pubmed
..Interestingly, these mutants grow well under microaerophilic conditions by overproducing the aerobic enzyme. Under such conditions wild-type bacteria turn off nrdAB and switch on nrdDG. ..
Carlson J, Fuchs J, Messing J. Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon. Proc Natl Acad Sci U S A. 1984;81:4294-7 pubmed
..6012 and 7139, encoding a 375-amino acid polypeptide with a molecular weight of 43,466, has been identified as the nrdB gene...