Gene Symbol: nhaR
Description: transcriptional activator of nhaA
Alias: ECK0021, JW0019, antO, yaaB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Mackie G. Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element. Nucleic Acids Res. 1986;14:6965-81 pubmed
    ..Thus it seems unlikely that the distal copy of IS1 plays any role in the termination or stability of mRNA transcribed from the gene for S20. ..
  2. Toesca I, Perard C, Bouvier J, Gutierrez C, Conter A. The transcriptional activator NhaR is responsible for the osmotic induction of osmC(p1), a promoter of the stress-inducible gene osmC in Escherichia coli. Microbiology. 2001;147:2795-803 pubmed
    ..The use of an E. coli genomic library showed that the cloned nhaR gene was able to stimulate transcription of an osmC-lac reporter fusion...
  3. Padan E, Gerchman Y, Rimon A, Rothman A, Dover N, Carmel Harel O. The molecular mechanism of regulation of the NhaA Na+/H+ antiporter of Escherichia coli, a key transporter in the adaptation to Na+ and H+. Novartis Found Symp. 1999;221:183-96; discussion 196-9 pubmed
    ..H225 and G338 are essential for the pH response of NhaA. nhaA transcription is dependent on NhaR, a positive regulator of the LysR family, and is regulated by Na+, the other environmental signal...
  4. Dover N, Padan E. Transcription of nhaA, the main Na(+)/H(+) antiporter of Escherichia coli, is regulated by Na(+) and growth phase. J Bacteriol. 2001;183:644-53 pubmed
    ..of nhaA, encoding the main Na(+)/H(+) antiporter of Escherichia coli, is induced by Na(+), regulated by NhaR, and affected by H-NS...
  5. Rahav Manor O, Carmel O, Karpel R, Taglicht D, Glaser G, Schuldiner S, et al. NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR), regulates nhaA, the sodium proton antiporter gene in Escherichia coli. J Biol Chem. 1992;267:10433-8 pubmed
    On the basis of protein homology, nhaR has previously been shown to belong to a large family of regulatory proteins, the LysR family (Henikoff, S., Haughn, G.W., Calvo, J.M., and Wallace, J.C. (1988) Proc. Natl. Acad. Sci. U. S. A...
  6. Carmel O, Rahav Manor O, Dover N, Shaanan B, Padan E. The Na+-specific interaction between the LysR-type regulator, NhaR, and the nhaA gene encoding the Na+/H+ antiporter of Escherichia coli. EMBO J. 1997;16:5922-9 pubmed
    We used partially purified NhaR and a highly purified His-tagged NhaR derivative to identify the cis-regulatory sequences of nhaA recognized by NhaR and to study the specific effect of Na+ on this interaction...
  7. Rowbury R, Goodson M, Humphrey T. Sodium chloride induces an NhaA/NhaR-independent acid sensitivity at neutral external pH in Escherichia coli. Appl Environ Microbiol. 1994;60:1630-4 pubmed
    ..Sensitization was not prevented by deletion of the nhaA, nhaR, or nhaB gene...
  8. Padan E, Schuldiner S. Na+/H+ antiporters, molecular devices that couple the Na+ and H+ circulation in cells. J Bioenerg Biomembr. 1993;25:647-69 pubmed
    ..A novel signal transduction to Na+ involving nhaR, a positive regulator, controls the expression of nhaA in E. coli...
  9. Cerca N, Jefferson K. Effect of growth conditions on poly-N-acetylglucosamine expression and biofilm formation in Escherichia coli. FEMS Microbiol Lett. 2008;283:36-41 pubmed publisher
    ..In this paper we investigated pga expression in wild type and csrA or nhaR deletion mutant strains during different growth phases and temperatures, and in response to chemical stimuli using ..

More Information


  1. Carmel O, Dover N, Rahav Manor O, Dibrov P, Kirsch D, Karpel R, et al. A single amino acid substitution (Glu134-->Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli. EMBO J. 1994;13:1981-9 pubmed
    The mutation nhaAup (antup) has now been identified as a Glu134 to Ala substitution in NhaR and designated nhaR1...
  2. Sturny R, Cam K, Gutierrez C, Conter A. NhaR and RcsB independently regulate the osmCp1 promoter of Escherichia coli at overlapping regulatory sites. J Bacteriol. 2003;185:4298-304 pubmed
    ..The distal promoter, osmCp1, is activated by NhaR and RcsB...
  3. Novichkov P, Laikova O, Novichkova E, Gelfand M, Arkin A, Dubchak I, et al. RegPrecise: a database of curated genomic inferences of transcriptional regulatory interactions in prokaryotes. Nucleic Acids Res. 2010;38:D111-8 pubmed publisher
    ..Another section in the database represents the results of accurate regulon propagation to the closely related genomes. ..
  4. Henikoff S, Haughn G, Calvo J, Wallace J. A large family of bacterial activator proteins. Proc Natl Acad Sci U S A. 1988;85:6602-6 pubmed
    ..The DNA database searching procedure and other methods used in this study should be useful in detecting other groups of related proteins. ..
  5. Padan E, Schuldiner S. Molecular physiology of the Na+/H+ antiporter in Escherichia coli. J Exp Biol. 1994;196:443-56 pubmed
    ..The adaptive response to Na+ is mediated by the positive regulator nhaR, which transduces the signal (intracellular Na+) to expression of the nhaA gene...
  6. Goller C, Wang X, Itoh Y, Romeo T. The cation-responsive protein NhaR of Escherichia coli activates pgaABCD transcription, required for production of the biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine. J Bacteriol. 2006;188:8022-32 pubmed
    ..We establish here that NhaR, a DNA-binding protein of the LysR family of transcriptional regulators, activates transcription of this operon...