Gene Symbol: nfi
Description: endonuclease V; deoxyinosine 3' endonuclease
Alias: ECK3990, JW5547, yjaF
Species: Escherichia coli str. K-12 substr. MG1655
Products:     nfi

Top Publications

  1. Miller M, Soufi B, Jers C, Blom N, Macek B, Mijakovic I. NetPhosBac - a predictor for Ser/Thr phosphorylation sites in bacterial proteins. Proteomics. 2009;9:116-25 pubmed publisher
    ..In conclusion, NetPhosBac clearly illustrates the advantage of taxa-specific predictors and we hope it will provide a useful asset to the microbiological community. ..
  2. Guo G, Ding Y, Weiss B. nfi, the gene for endonuclease V in Escherichia coli K-12. J Bacteriol. 1997;179:310-6 pubmed
    ..The protein was purified to apparent homogeneity, and from its N-terminal sequence, its gene, nfi, was identified. nfi is immediately downstream of hemE, at kb 4208 (90...
  3. Gates F, Linn S. Endonuclease V of Escherichia coli. J Biol Chem. 1977;252:1647-53 pubmed
    ..We suggest that this enzyme be designated as endonuclease V of E. coli. ..
  4. Majorek K, Bujnicki J. Modeling of Escherichia coli Endonuclease V structure in complex with DNA. J Mol Model. 2009;15:173-82 pubmed publisher
    ..The modeled structure provides a convenient tool to study protein sequence-structure-function relationships in EndoV and to engineer its further variants. ..
  5. Yao M, Kow Y. Cleavage of insertion/deletion mismatches, flap and pseudo-Y DNA structures by deoxyinosine 3'-endonuclease from Escherichia coli. J Biol Chem. 1996;271:30672-6 pubmed
    ..These biochemical properties suggest that deoxyinosine 3'-endonuclease might be important in the repair of IDM structures generated in lagging strand during DNA replication. ..
  6. Budke B, Kuzminov A. Hypoxanthine incorporation is nonmutagenic in Escherichia coli. J Bacteriol. 2006;188:6553-60 pubmed
    Endonuclease V, encoded by the nfi gene, initiates removal of the base analogs hypoxanthine and xanthine from DNA, acting to prevent mutagenesis from purine base deamination within the DNA...
  7. Weiss B. Endonuclease V of Escherichia coli prevents mutations from nitrosative deamination during nitrate/nitrite respiration. Mutat Res. 2001;461:301-9 pubmed
    ..In this study, the protective effect of Endo V was observed under such conditions. During micro-aerobic growth, an nfi (Endo V) mutation enhanced the frequency of nitrate- and nitrite-induced A:T-->G:C and G:C-->A:T transition ..
  8. Demple B, Gates F, Linn S. Purification and properties of Escherichia coli endodeoxyribonuclease V. Methods Enzymol. 1980;65:224-31 pubmed
  9. Kwon Y, Weiss B. Production of 3-nitrosoindole derivatives by Escherichia coli during anaerobic growth. J Bacteriol. 2009;191:5369-76 pubmed publisher
    ..An nfi (endonuclease V) mutant and a recA mutant were selectively killed during the period of chromophore production, and ..

More Information


  1. Guo G, Weiss B. Endonuclease V (nfi) mutant of Escherichia coli K-12. J Bacteriol. 1998;180:46-51 pubmed
    Endonuclease V (deoxyinosine 3' endonuclease), the product of the nfi gene, has a specificity that encompasses DNAs containing dIMP, abasic sites, base mismatches, uracil, and even untreated single-stranded DNA...
  2. Schouten K, Weiss B. Endonuclease V protects Escherichia coli against specific mutations caused by nitrous acid. Mutat Res. 1999;435:245-54 pubmed
    ..An endonuclease V (nfi) mutation was tested for specific mutator effects on a battery of trp and lac mutant alleles...
  3. Blackmore R, Linn S. Partial purification and characterization of four endodeoxyribonuclease activities from Escherichia coli K-12. Nucleic Acids Res. 1974;1:1-17 pubmed
    ..Irradiation of the RFI with ultraviolet light markedly increases the rate of hydrolysis by A(3). B appears to form 3'-phosphoryl termini with fd DNA, but its characterization is highly preliminary due to its instability. ..
  4. Burgis N, Brucker J, Cunningham R. Repair system for noncanonical purines in Escherichia coli. J Bacteriol. 2003;185:3101-10 pubmed
    ..Disruption of the endonuclease V gene, nfi, rescues the HAP sensitivity displayed by moa and moa rdgB mutants and reduces the level of recombination and SOS ..
  5. Bradshaw J, Kuzminov A. RdgB acts to avoid chromosome fragmentation in Escherichia coli. Mol Microbiol. 2003;48:1711-25 pubmed
    ..We find that DNA isolated from rdgB mutants contains EndoV-recognizable modifications, whereas DNA from nfi mutants does not, substantiating the dITP/dXTP interception by RdgB...
  6. Yao M, Kow Y. Further characterization of Escherichia coli endonuclease V. Mechanism of recognition for deoxyinosine, deoxyuridine, and base mismatches in DNA. J Biol Chem. 1997;272:30774-9 pubmed
    ..The gene coding for the enzyme was identified to be orf 225 or nfi (endonuclease five)...
  7. Yao M, Hatahet Z, Melamede R, Kow Y. Purification and characterization of a novel deoxyinosine-specific enzyme, deoxyinosine 3' endonuclease, from Escherichia coli. J Biol Chem. 1994;269:16260-8 pubmed
    ..Therefore, deoxyinosine 3' endonuclease recognizes, but does not remove, the lesion from the DNA molecule. The biological significance of this novel activity is discussed with reference to other repair activities in E. coli. ..
  8. Yao M, Kow Y. Strand-specific cleavage of mismatch-containing DNA by deoxyinosine 3'-endonuclease from Escherichia coli. J Biol Chem. 1994;269:31390-6 pubmed
  9. Demple B, Linn S. On the recognition and cleavage mechanism of Escherichia coli endodeoxyribonuclease V, a possible DNA repair enzyme. J Biol Chem. 1982;257:2848-55 pubmed
    ..Endonuclease V seems well suited to act as a DNA repair enzyme, surveying the genome for structural distortions generated by lesions for which specific repair systems might not exist. ..