narP

Summary

Gene Symbol: narP
Description: response regulator in two-component regulatory system with NarQ
Alias: ECK2185, JW2181
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Stewart V, Bledsoe P. Substitutions at auxiliary operator O3 enhance repression by nitrate-responsive regulator NarL at synthetic lac control regions in Escherichia coli K-12. J Bacteriol. 2008;190:428-33 pubmed
    ..We constructed monocopy lac operon control regions in which the operators O1-lac and O3-lac were replaced by NarL and NarP binding sites from the nirB or napF operon control regions...
  2. Rabin R, Stewart V. Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12. J Bacteriol. 1993;175:3259-68 pubmed
    ..We designate this second regulator NarP. We isolated insertion mutants that are defective in aeg-46.5 operon expression...
  3. Wang H, Gunsalus R. Coordinate regulation of the Escherichia coli formate dehydrogenase fdnGHI and fdhF genes in response to nitrate, nitrite, and formate: roles for NarL and NarP. J Bacteriol. 2003;185:5076-85 pubmed
    ..Consistent with prior reports, NarL was able to induce fdnG-lacZ expression. However, NarP could not induce expression; rather, it functioned as an antagonist of fdnG-lacZ expression under low-nitrate ..
  4. Browning D, Grainger D, Beatty C, Wolfe A, Cole J, Busby S. Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression. Mol Microbiol. 2005;57:496-510 pubmed
    ..triggered transcription factor, FNR, and by the nitrate/nitrite ion-controlled response regulators, NarL or NarP, but is repressed by the IHF and Fis proteins...
  5. Darwin A, Tyson K, Busby S, Stewart V. Differential regulation by the homologous response regulators NarL and NarP of Escherichia coli K-12 depends on DNA binding site arrangement. Mol Microbiol. 1997;25:583-95 pubmed
    The NarL and NarP proteins are homologous response regulators of Escherichia coli that control the expression of several operons in response to nitrate and nitrite...
  6. Lin H, Bledsoe P, Stewart V. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen- responsive regulator Fnr in Escherichia coli K-12. J Bacteriol. 2007;189:7539-48 pubmed
    ..Nitrate and nitrite regulate anaerobic respiratory gene expression through the NarX-NarL and NarQ-NarP two-component systems. All known Nar-activated genes also require the oxygen-responsive Fnr transcription activator...
  7. Stewart V, Bledsoe P. Synthetic lac operator substitutions for studying the nitrate- and nitrite-responsive NarX-NarL and NarQ-NarP two-component regulatory systems of Escherichia coli K-12. J Bacteriol. 2003;185:2104-11 pubmed
    The NarX and NarQ sensor-histidine kinases control phosphorylation of the NarL and NarP response regulators in response to the respiratory oxidants nitrate and nitrite...
  8. Favorov A, Gelfand M, Gerasimova A, Ravcheev D, Mironov A, Makeev V. A Gibbs sampler for identification of symmetrically structured, spaced DNA motifs with improved estimation of the signal length. Bioinformatics. 2005;21:2240-5 pubmed
    ..the examples studied, ArcA binding sites were demonstrated to have the structure of a direct spaced repeat, whereas NarP binding sites exhibited the palindromic structure. The WWW interface of the program, its FreeBSD (4...
  9. Noriega C, Lin H, Chen L, Williams S, Stewart V. Asymmetric cross-regulation between the nitrate-responsive NarX-NarL and NarQ-NarP two-component regulatory systems from Escherichia coli K-12. Mol Microbiol. 2010;75:394-412 pubmed publisher
    The NarX-NarL and NarQ-NarP sensor-response regulator pairs control Escherichia coli gene expression in response to nitrate and nitrite. Previous analysis suggests that the Nar two-component systems form a cross-regulation network in vivo...

More Information

Publications22

  1. Stewart V, Bledsoe P. Fnr-, NarP- and NarL-dependent regulation of transcription initiation from the Haemophilus influenzae Rd napF (periplasmic nitrate reductase) promoter in Escherichia coli K-12. J Bacteriol. 2005;187:6928-35 pubmed publisher
    ..coli napF operon control region is activated by the Fnr protein in response to anaerobiosis and by the NarQ-NarP two-component regulatory system in response to nitrate or nitrite...
  2. Pao G, Saier M. Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution. J Mol Evol. 1995;40:136-54 pubmed
    ..Sequence data were correlated with structure/function when such information was available.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  3. Lin A, Stewart V. Functional roles for the GerE-family carboxyl-terminal domains of nitrate response regulators NarL and NarP of Escherichia coli K-12. Microbiology. 2010;156:2933-43 pubmed publisher
    NarL and NarP are paralogous response regulators that control anaerobic gene expression in response to the favoured electron acceptors nitrate and nitrite...
  4. Partridge J, Browning D, Xu M, Newnham L, Scott C, Roberts R, et al. Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP. Microbiology. 2008;154:608-18 pubmed publisher
    ..The latter heptamers are arranged as a 7-2-7 inverted repeat, which is required for recognition by NarP. Accordingly, NarP protected the 7-2-7 region in DNase I footprints, and mutation of either heptamer +6 or heptamer ..
  5. Browning D, Lee D, Wolfe A, Cole J, Busby S. The Escherichia coli K-12 NarL and NarP proteins insulate the nrf promoter from the effects of integration host factor. J Bacteriol. 2006;188:7449-56 pubmed
    ..host factor (IHF) binding at position -54, and this suppression is counteracted by binding of the NarL or NarP response regulator at position -74.5. The E...
  6. Constantinidou C, Hobman J, Griffiths L, Patel M, Penn C, Cole J, et al. A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth. J Biol Chem. 2006;281:4802-15 pubmed
    ..The narP gene was also deleted from the narXL mutant to reveal the extent of regulation by phosphorylated NarP...
  7. Stock J, Stock A, Mottonen J. Signal transduction in bacteria. Nature. 1990;344:395-400 pubmed publisher
    ..This phosphotransfer network couples environmental signals to an array of response elements that control cell motility and regulate gene expression...
  8. Aiso T, Ohki R. Instability of sensory histidine kinase mRNAs in Escherichia coli. Genes Cells. 2003;8:179-87 pubmed
    ..Monocistronic transcripts of the cognate response regulator genes, arcA, rcsB, narP and narL have a half-life of 1.5-3.4 min. mRNAs of the six histidine kinase genes in E...
  9. Stewart V. Nitrate regulation of anaerobic respiratory gene expression in Escherichia coli. Mol Microbiol. 1993;9:425-34 pubmed
    ..The homologous protein NARP controls nitrite induction of at least two operons...
  10. Parkinson J. Signal transduction schemes of bacteria. Cell. 1993;73:857-71 pubmed
  11. Whitehead R, Cole J. Different responses to nitrate and nitrite by the model organism Escherichia coli and the human pathogen Neisseria gonorrhoeae. Biochem Soc Trans. 2006;34:111-4 pubmed
    ..during anaerobic growth is mediated by the dual-acting two-component regulatory systems NarX-NarL and NarQ-NarP. In contrast, Neisseria gonorrhoeae responds only to nitrite: it expresses only NarQ-NarP...
  12. Ravcheev D, Gerasimova A, Mironov A, Gelfand M. Comparative genomic analysis of regulation of anaerobic respiration in ten genomes from three families of gamma-proteobacteria (Enterobacteriaceae, Pasteurellaceae, Vibrionaceae). BMC Genomics. 2007;8:54 pubmed
    ..Thus, in E. coli, global control of respiration is mediated by four transcription factors, Fnr, ArcA, NarL and NarP. However, in other Gamma-proteobacteria the composition of global respiration regulators may be different.
  13. Parkinson J, Kofoid E. Communication modules in bacterial signaling proteins. Annu Rev Genet. 1992;26:71-112 pubmed