mutY

Summary

Gene Symbol: mutY
Description: adenine DNA glycosylase
Alias: ECK2956, JW2928, micA, mutB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Participation of BER and NER pathways in the repair of DNA lesions induced at low N-nitrosodiethylamine concentrations
    Claudia Alessandra Fortes Aiub
    Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av 28 de Setembro, 87 fds, 4 andar, 20551 030 Rio de Janeiro, Brazil
    Toxicol Lett 154:133-42. 2004
  2. ncbi The C-terminal domain of MutY glycosylase determines the 7,8-dihydro-8-oxo-guanine specificity and is crucial for mutation avoidance
    X Li
    Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, Baltimore, Maryland 21201, USA
    J Biol Chem 275:8448-55. 2000
  3. ncbi MutY and MutY homologs (MYH) in genome maintenance
    A Lien Lu
    Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, Maryland 21201, USA
    Front Biosci 11:3062-80. 2006
  4. ncbi A pair of membrane-embedded acidic residues in the NuoK subunit of Escherichia coli NDH-1, a counterpart of the ND4L subunit of the mitochondrial complex I, are required for high ubiquinone reductase activity
    Marko Kervinen
    Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland
    Biochemistry 43:773-81. 2004
  5. ncbi Reaction intermediates in the catalytic mechanism of Escherichia coli MutY DNA glycosylase
    Raymond C Manuel
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1071, USA
    J Biol Chem 279:46930-9. 2004
  6. pmc Competition between MutY and mismatch repair at A x C mispairs In vivo
    Mandy Kim
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095, USA
    J Bacteriol 185:4626-9. 2003
  7. pmc Efficient recognition of substrates and substrate analogs by the adenine glycosylase MutY requires the C-terminal domain
    N H Chmiel
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112 0850, USA
    Nucleic Acids Res 29:553-64. 2001
  8. ncbi Insight into the roles of tyrosine 82 and glycine 253 in the Escherichia coli adenine glycosylase MutY
    Alison L Livingston
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112, USA
    Biochemistry 44:14179-90. 2005
  9. ncbi A substrate recognition role for the [4Fe-4S]2+ cluster of the DNA repair glycosylase MutY
    S L Porello
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 37:6465-75. 1998
  10. pmc Mutation rate dynamics in a bacterial population reflect tension between adaptation and genetic load
    Sébastien Wielgoss
    Laboratoire Adaptation et Pathogénie des Micro Organismes, Universite Joseph Fourier Grenoble, F 38042 Grenoble Cedex 9, France
    Proc Natl Acad Sci U S A 110:222-7. 2013

Research Grants

  1. REPAIR OF OXIDATIVELY DAMAGED GUANINES IN HUMAN
    A Lien Lu Chang; Fiscal Year: 2007
  2. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 2001
  3. REPAIR OF OXIDATIVELY DAMAGED GUANINES IN HUMAN
    A Lien Lu Chang; Fiscal Year: 2002
  4. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 2006
  5. Repair of Oxidatively Damaged Guanines
    A LIEN L LU CHANG; Fiscal Year: 2010
  6. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 1993
  7. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 1992
  8. Repair of Oxidatively Damaged Guanines
    A LIEN L LU CHANG; Fiscal Year: 2010
  9. Pathway of Spontaneous Mutagenesis in Escherichia coli
    Robert Fowler; Fiscal Year: 2004

Scientific Experts

  • A Lien Lu
  • Robert G Fowler
  • Po Wen Chang
  • Dee R Denver
  • A Lien Lu Chang
  • A LIEN L LU CHANG
  • Sheila S David
  • Amie K Boal
  • Alison L Livingston
  • Jacqueline K Barton
  • Sucharita Kundu
  • Anthony W Francis
  • Haibo Bai
  • Lucinda Notley-McRobb
  • Sébastien Wielgoss
  • X Li
  • Patricia Auffret van der Kemp
  • C M Gifford
  • Yaroslava Y Polosina
  • Mary Ann Pope
  • Karam S Boparai
  • Jong Chin Lin
  • Tetsuya Suzuki
  • Damian Gawel
  • Sarah Delaney
  • Ella Rotman
  • Valerie L O'Shea
  • A Lien Lu-Chang
  • Eylon Yavin
  • John M Essigmann
  • Eric T Kool
  • Joanna Klapacz
  • Marko Kervinen
  • Jessica L Kosa
  • Raymond C Manuel
  • Anton B Guliaev
  • Svitlana Malyarchuk
  • Claudia Alessandra Fortes Aiub
  • Chih Yung Lee
  • S S David
  • R Stephen Lloyd
  • Francoise Dantzer
  • Sun Hee Yoon
  • Joe A B McCann
  • Mandy Kim
  • Lina Li
  • Ana M Sanchez
  • Elizabeth M Boon
  • Troy E Messick
  • Thomas Ferenci
  • Shona Seeto
  • Andrew S Bernards
  • K Hashiguchi
  • Stephane Cruveiller
  • Dominique Schneider
  • Olivier Tenaillon
  • Jeffrey E Barrick
  • Richard E Lenski
  • Claudine Medigue
  • Béatrice Chane-Woon-Ming
  • W James Dittmar
  • Michael J Wiser
  • N H Chmiel
  • P G House
  • Helle D Ulrich
  • Pamela A Sontz
  • R C Manuel
  • Dianne K Newman
  • Megan K Brinkmeyer
  • Guenaelle Burguiere-Slezak
  • Claire G Cupples
  • Serge Boiteux
  • Photini Pitsikas
  • Justin Mui
  • Joseph C Genereux
  • Jeffrey A Gralnick
  • S S Wallace
  • Marcelo de Padula
  • A R Timms
  • Roel M Schaaper
  • Josbert J Keller
  • Mirjam M Polak
  • Joep F W M Bartelsman
  • Evelien Dekker
  • Elisbeth M H Mathus-Vliegen
  • Hiroyuki Kamiya
  • Carel J M van Noesel
  • Daniel L Cox
  • Phuong T Pham
  • Rajiv R P Singh

Detail Information

Publications69

  1. ncbi Participation of BER and NER pathways in the repair of DNA lesions induced at low N-nitrosodiethylamine concentrations
    Claudia Alessandra Fortes Aiub
    Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av 28 de Setembro, 87 fds, 4 andar, 20551 030 Rio de Janeiro, Brazil
    Toxicol Lett 154:133-42. 2004
    ..and double Escherichia coli DNA repair mutants (uvrA, uvrB, uvrC, fpg, nth, xthA, fpg/nth, uvrA/fpg, fpg/xthA, mutY, and fpg/mutY), using pre-incubation periods of 90 min...
  2. ncbi The C-terminal domain of MutY glycosylase determines the 7,8-dihydro-8-oxo-guanine specificity and is crucial for mutation avoidance
    X Li
    Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, Baltimore, Maryland 21201, USA
    J Biol Chem 275:8448-55. 2000
    Escherichia coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA...
  3. ncbi MutY and MutY homologs (MYH) in genome maintenance
    A Lien Lu
    Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, Maryland 21201, USA
    Front Biosci 11:3062-80. 2006
    The base excision repair carried out by bacterial MutY DNA glycosylase and eukaryotic MutY homolog (MYH) is responsible for removing adenines misincorporated into DNA opposite G and 7,8-dihydro-8-oxo-guanines (8-oxoG); thereby preventing ..
  4. ncbi A pair of membrane-embedded acidic residues in the NuoK subunit of Escherichia coli NDH-1, a counterpart of the ND4L subunit of the mitochondrial complex I, are required for high ubiquinone reductase activity
    Marko Kervinen
    Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland
    Biochemistry 43:773-81. 2004
    ....
  5. ncbi Reaction intermediates in the catalytic mechanism of Escherichia coli MutY DNA glycosylase
    Raymond C Manuel
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1071, USA
    J Biol Chem 279:46930-9. 2004
    The Escherichia coli adenine DNA glycosylase, MutY, plays an important role in the maintenance of genomic stability by catalyzing the removal of adenine opposite 8-oxo-7,8-dihydroguanine or guanine in duplex DNA...
  6. pmc Competition between MutY and mismatch repair at A x C mispairs In vivo
    Mandy Kim
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095, USA
    J Bacteriol 185:4626-9. 2003
    We show that the MutY protein competes with the MutS-dependent mismatch repair system to process at least some A. C mispairs in vivo, converting them to G. C pairs...
  7. pmc Efficient recognition of substrates and substrate analogs by the adenine glycosylase MutY requires the C-terminal domain
    N H Chmiel
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112 0850, USA
    Nucleic Acids Res 29:553-64. 2001
    The Escherichia coli DNA repair enzyme MutY plays an important role in the prevention of DNA mutations by removing misincorporated adenine residues from 7, 8-dihydro-8-oxo-2'-deoxyguanosine:2'-deoxyadenosine (OG:A) mispairs...
  8. ncbi Insight into the roles of tyrosine 82 and glycine 253 in the Escherichia coli adenine glycosylase MutY
    Alison L Livingston
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112, USA
    Biochemistry 44:14179-90. 2005
    ..The Escherichia coli base excision repair glycosylase MutY plays an important role in preventing OG-associated mutations by removing adenines misincorporated opposite OG ..
  9. ncbi A substrate recognition role for the [4Fe-4S]2+ cluster of the DNA repair glycosylase MutY
    S L Porello
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 37:6465-75. 1998
    The Escherichia coli DNA repair enzyme MutY plays an important role in the recognition and repair of 7, 8-dihydro-8-oxo-2'-deoxyguanosine: 2'-deoxyadenosine (OG:A) mismatches in DNA [Michaels et al. (1992) Proc. Natl. Acad. Sci. U.S. A...
  10. pmc Mutation rate dynamics in a bacterial population reflect tension between adaptation and genetic load
    Sébastien Wielgoss
    Laboratoire Adaptation et Pathogénie des Micro Organismes, Universite Joseph Fourier Grenoble, F 38042 Grenoble Cedex 9, France
    Proc Natl Acad Sci U S A 110:222-7. 2013
    ..coli population that first evolved a mutT hypermutator phenotype was later invaded by two independent lineages with mutY mutations that reduced genome-wide mutation rates...
  11. ncbi DNA-bound redox activity of DNA repair glycosylases containing [4Fe-4S] clusters
    Amie K Boal
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Biochemistry 44:8397-407. 2005
    b>MutY and endonuclease III, two DNA glycosylases from Escherichia coli, and AfUDG, a uracil DNA glycosylase from Archeoglobus fulgidus, are all base excision repair enzymes that contain the [4Fe-4S](2+) cofactor...
  12. ncbi An Escherichia coli MutY mutant without the six-helix barrel domain is a dimer in solution and assembles cooperatively into multisubunit complexes with DNA
    Chih Yung Lee
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 279:52653-63. 2004
    Escherichia coli MutY is an adenine and weak guanine DNA glycosylase involved in reducing the mutagenic effects of 7,8-dihydro-8-oxoguanine (GO)...
  13. ncbi Transcription promotes guanine to thymine mutations in the non-transcribed strand of an Escherichia coli gene
    Joanna Klapacz
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    DNA Repair (Amst) 4:806-13. 2005
    ..Furthermore, in both mutY and mutM mutY backgrounds the mutations were overwhelmingly G:C to T:A...
  14. pmc Physical and functional interactions between Escherichia coli MutY and endonuclease VIII
    A Lien Lu
    Department of Biochemistry and Molecular Biology and Greenebaum Cancer Center, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Biochem J 393:381-7. 2006
    ..In Escherichia coli, MutY DNA glycosylase can remove misincorporated adenine opposite G or GO on the template strand during DNA replication...
  15. ncbi RecN and RecG are required for Escherichia coli survival of Bleomycin-induced damage
    Jessica L Kosa
    Biological Engineering Division, Department of Chemistry, Massachusetts Institute of Technology, Rm 56 689, 77 Massachusetts Avenue, Cambridge 02139, USA
    Mutat Res 554:149-57. 2004
    ..Escherichia coli recA cells were far more sensitive than were uvrA, dam-3, and mutM mutY strains, underscoring the importance of RecA to survival...
  16. ncbi Chloroethylnitrosourea-derived ethano cytosine and adenine adducts are substrates for Escherichia coli glycosylases excising analogous etheno adducts
    Anton B Guliaev
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    DNA Repair (Amst) 3:1311-21. 2004
    ..However, also as shown by MD, the stacking interaction between the EC base and Phe 30 in the Mug active site is reduced as compared to the epsilonC base, which could account for the lower EC activity observed in this study...
  17. ncbi Initiation of repair of A/G mismatches is modulated by sequence context
    Ana M Sanchez
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, TX 77555 1071, USA
    DNA Repair (Amst) 2:863-78. 2003
    ..mismatch, in which the recognition and excision of adenine from the mismatch is mediated by the Escherichia coli MutY enzyme, not only does the local sequence context affect the strength of base stacking interactions, but it also ..
  18. pmc Two clustered 8-oxo-7,8-dihydroguanine (8-oxodG) lesions increase the point mutation frequency of 8-oxodG, but do not result in double strand breaks or deletions in Escherichia coli
    Svitlana Malyarchuk
    Department of Molecular and Cellular Physiology and the Feist Weiller Cancer Center, Louisiana Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA
    Nucleic Acids Res 32:5721-31. 2004
    ..The 8-oxodG mutation frequency declined in MutY-deficient bacteria when the opposing 8-oxodG was 6 bp away, and was similar to a single 8-oxodG when the lesions ..
  19. ncbi Isolation and analyses of MutY homologs (MYH)
    A Lien Lu-Chang
    University of Maryland, Department of Biochemistry, Baltimore, USA
    Methods Enzymol 408:64-78. 2006
    The base excision repair carried out by the bacterial MutY DNA glycosylase and eukaryotic MutY homolog (MYH) is responsible for removing adenines misincorporated into DNA opposite 7,8-dihydro-8-oxo-guanines (8-oxoG), thereby preventing G:..
  20. pmc Adenine removal activity and bacterial complementation with the human MutY homologue (MUTYH) and Y165C, G382D, P391L and Q324R variants associated with colorectal cancer
    Sucharita Kundu
    Department of Chemistry, University of California Davis, One Shields Avenue, Davis, CA 95616, USA
    DNA Repair (Amst) 8:1400-10. 2009
    ..In addition, the ability of MUTYH and the variants to suppress mutations and complement for the absence of MutY in Escherichia coli was assessed using rifampicin resistance assays...
  21. pmc Redox signaling between DNA repair proteins for efficient lesion detection
    Amie K Boal
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 106:15237-42. 2009
    ..transport (CT) to mismatched and damaged base pairs, we have proposed that DNA repair glycosylases (EndoIII and MutY) containing a redox-active [4Fe4S] cluster could use DNA CT in signaling one another to search cooperatively for ..
  22. pmc Insights into the role of Val45 and Gln182 of Escherichia coli MutY in DNA substrate binding and specificity
    Po Wen Chang
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, 108 North Greene Street, Baltimore, MD 21201, USA
    BMC Biochem 10:19. 2009
    Escherichia coli MutY (EcMutY) reduces mutagenesis by removing adenines paired with guanines or 7,8-dihydro-8-oxo-guanines (8-oxoG). V45 and Q182 of EcMutY are considered to be the key determinants of adenine specificity...
  23. pmc The Escherichia coli mismatch repair protein MutL recruits the Vsr and MutH endonucleases in response to DNA damage
    Yaroslava Y Polosina
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada
    J Bacteriol 191:4041-3. 2009
    ..The interaction of MutL with each enzyme is enhanced in vivo by 2-aminopurine treatment and by inactivation of the mutY gene. We hypothesize that MutL recruits the endonucleases to sites of DNA damage.
  24. pmc PCNA monoubiquitylation and DNA polymerase eta ubiquitin-binding domain are required to prevent 8-oxoguanine-induced mutagenesis in Saccharomyces cerevisiae
    Patricia Auffret van der Kemp
    CEA, IRCM, UMR217 CNRS Radiobiologie Moléculaire et Cellulaire, 18 route du Panorama, BP6, 92265 Fontenay aux Roses, France
    Nucleic Acids Res 37:2549-59. 2009
    ..This study suggests that Pol eta and the post-replication repair (PRR) machinery can also prevent mutagenesis at DNA lesions that do not stall replication forks...
  25. doi Hyperplastic polyps and sessile serrated adenomas as a phenotypic expression of MYH-associated polyposis
    Karam S Boparai
    Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands
    Gastroenterology 135:2014-8. 2008
    ..We examined the presence of HPs and sessile serrated adenomas (SSAs) in 17 MAP patients and studied the occurrence of G:C-->T:A transversions in the APC and K-ras gene in these polyps...
  26. pmc Theoretical study of DNA damage recognition via electron transfer from the [4Fe-4S] complex of MutY
    Jong Chin Lin
    Department of Physics, University of California, Davis, California 95616, USA
    Biophys J 95:3259-68. 2008
    The mechanism of site-specific recognition of DNA by proteins has been a long-standing issue. The DNA glycosylase MutY, for instance, must find the rare 8-oxoguanine-adenine mismatches among the large number of basepairs in the DNA...
  27. pmc Role of accessory DNA polymerases in DNA replication in Escherichia coli: analysis of the dnaX36 mutator mutant
    Damian Gawel
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    J Bacteriol 190:1730-42. 2008
    ..The present results, including lack of interaction of dnaX36 with mutM, mutY, and recA defects, support our assumption that dnaX36-mediated mutations originate as errors of replication rather ..
  28. pmc Unnatural substrates reveal the importance of 8-oxoguanine for in vivo mismatch repair by MutY
    Alison L Livingston
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112, USA
    Nat Chem Biol 4:51-8. 2008
    Escherichia coli MutY has an important role in preventing mutations associated with the oxidative lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA by excising adenines from OG.A mismatches as the first step of base excision repair...
  29. ncbi Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP
    Tetsuya Suzuki
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    DNA Repair (Amst) 7:88-94. 2008
    ..were introduced into Escherichia coli strains deficient in endonucleases III (Nth) and VIII (Nei) and MutY, and mutations in the chromosomal rpoB gene were analyzed...
  30. pmc The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications
    Ella Rotman
    Department of Microbiology, University of Illinois at Urbana Champaign, IL 61801 3709, USA
    J Bacteriol 189:6976-88. 2007
    ..Overproduction of the MutM and MutY DNA glycosylases, both acting on DNA containing 8-oxo-G, still yields no lethality in mutT recBC double mutants...
  31. pmc DNA repair glycosylases with a [4Fe-4S] cluster: a redox cofactor for DNA-mediated charge transport?
    Amie K Boal
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    J Inorg Biochem 101:1913-21. 2007
    ..When studied bound to DNA, two of these repair proteins (MutY and Endonuclease III from Escherichia coli) display DNA-dependent reversible electron transfer with characteristics ..
  32. ncbi The substrate specificity of MutY for hyperoxidized guanine lesions in vivo
    Sarah Delaney
    Department of Chemistry and Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 46:1448-55. 2007
    ..One of these enzymes, MutY, excises an A mispaired with 8-oxoG as part of the process to restore the original G:C base pair...
  33. pmc Physical and functional interactions between Escherichia coli MutY glycosylase and mismatch repair protein MutS
    Haibo Bai
    Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201, USA
    J Bacteriol 189:902-10. 2007
    Escherichia coli MutY and MutS increase replication fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines (8-oxoG), G, or C...
  34. ncbi An evolutionary analysis of the helix-hairpin-helix superfamily of DNA repair glycosylases
    Dee R Denver
    Department of Biology, Indiana University, USA
    Mol Biol Evol 20:1603-11. 2003
    ..Six HhH gene families (named AlkA: alkyladenine glycosylase; MpgII: N-methylpurine glycosylase II; MutY/Mig: A/G-specific adenine glycosylase/mismatch glycosylase; Nth: endonuclease III; OggI: 8-oxoguanine glycosylase I;..
  35. pmc The C-terminal domain of Escherichia coli MutY is involved in DNA binding and glycosylase activities
    Lina Li
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Nucleic Acids Res 31:3038-49. 2003
    Escherichia coli MutY is an adenine and a weak guanine DNA glycosylase involved in reducing mutagenic effects of 7,8-dihydro-8-oxo-guanine (8-oxoG)...
  36. pmc Multiprobe RNase protection assay analysis of mRNA levels for the Escherichia coli oxidative DNA glycosylase genes under conditions of oxidative stress
    C M Gifford
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Burlington, Vermont 05405 0068, USA
    J Bacteriol 182:5416-24. 2000
    Escherichia coli formamidopyrimidine DNA glycosylase (Fpg), MutY DNA glycosylase, endonuclease VIII, and endonuclease III are oxidative base excision repair DNA glycosylases that remove oxidized bases from DNA, or an incorrect base paired ..
  37. ncbi A UmuD,C-dependent pathway for spontaneous G:C to C:G transversions in stationary phase Escherichia coli mut Y
    A R Timms
    MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, UK
    Mutat Res 435:77-80. 1999
    In Escherichia coli trpA23 bacteria lacking the MutY glycosylase and incubated on plates in the absence of tryptophan, tryptophan-independent mutants continue to arise during incubation over many days...
  38. pmc The genes encoding formamidopyrimidine and MutY DNA glycosylases in Escherichia coli are transcribed as part of complex operons
    C M Gifford
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Burlington, Vermont 05405 0068, USA
    J Bacteriol 181:4223-36. 1999
    Escherichia coli formamidopyrimidine (Fpg) DNA glycosylase and MutY DNA glycosylase are base excision repair proteins that work together to protect cells from the mutagenic effects of the commonly oxidized guanine product 7,8-dihydro-8-..
  39. pmc Evidence that MutY is a monofunctional glycosylase capable of forming a covalent Schiff base intermediate with substrate DNA
    S D Williams
    Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA
    Nucleic Acids Res 26:5123-33. 1998
    The Escherichia coli adenine glycosylase MutY is involved in the repair of 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG):A and G:A mispairs in DNA...
  40. ncbi MutY DNA glycosylase: base release and intermediate complex formation
    D O Zharkov
    Department of Pharmacological Sciences, State University of New York at Stony Brook 11794l 8651, USA
    Biochemistry 37:12384-94. 1998
    b>MutY protein, a DNA glycosylase found in Escherichia coli, recognizes dA:dG, dA:8-oxodG, and dA:dC mismatches in duplex DNA, excising the adenine moiety...
  41. ncbi Substrate specificity of Escherichia coli MutY protein
    N V Bulychev
    Department of Pharmacological Sciences, State University of New York at Stony Brook 11794 8651, USA
    Biochemistry 35:13147-56. 1996
    The MutY protein of Escherichia coli removes mismatched deoxyadenine residues from DNA. In this study, duplex oligodeoxynucleotides containing modified bases are used as model substrates for this enzyme...
  42. pmc Patch length of localized repair events: role of DNA polymerase I in mutY-dependent mismatch repair
    J P Radicella
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    J Bacteriol 175:7732-6. 1993
    In vivo experiments with heteroduplex lambda genomes show that the MutY mismatch repair system of Escherichia coli defines an average repair tract that is shorter than 27 nucleotides and longer than 9 nucleotides and extends 3' from the ..
  43. ncbi DNA determinants and substrate specificities of Escherichia coli MutY
    A L Lu
    Department of Biological Chemistry, School of Medicine, University of Maryland, Baltimore 21201, USA
    J Biol Chem 270:23582-8. 1995
    Potential DNA contacts involved in the specific interaction between the Escherichia coli MutY protein and a 40-mer oligonucleotide containing an A/G mismatch have been examined by alkylation interference techniques...
  44. pmc Mutator gene of Escherichia coli B
    E C Siegel
    J Bacteriol 94:38-47. 1967
    ..No suppression was found. Recombinants were prepared containing mutS1 and the Treffers mutator gene of E. coli K-12. The effect of the mutator genes appears to be additive...
  45. pmc Selection of a mutant of Escherichia coli which has high mutation rates
    R B Helling
    J Bacteriol 96:975-80. 1968
    ..This mutation (mut-2) and the Treffers' mutation (mutT1) mapped at approximately the same position to the right of leu. Specificity studies showed that the two mutations differ in rates of mutation produced...
  46. pmc The mutY gene: a mutator locus in Escherichia coli that generates G.C----T.A transversions
    Y Nghiem
    Molecular Biology Institute, University of California, Los Angeles 90024
    Proc Natl Acad Sci U S A 85:2709-13. 1988
    ..The locus responsible for the mutator phenotype is designated mutY and maps near 64 min on the genetic map of E. coli...
  47. pmc Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs
    K G Au
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710
    Proc Natl Acad Sci U S A 86:8877-81. 1989
    Mutations in the mutY gene of Escherichia coli confer hypermutability reflecting G.C to T.A transversion mutations and result in a deficiency in methyl-independent G-A to G.C mismatch correction...
  48. pmc MutY, an adenine glycosylase active on G-A mispairs, has homology to endonuclease III
    M L Michaels
    Molecular Biology Institute, University of California, Los Angeles 90024
    Nucleic Acids Res 18:3841-5. 1990
    The mutY gene of Escherichia coli, which codes for an adenine glycosylase that excises the adenine of a G-A mispair, has been cloned and sequenced...
  49. pmc Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY
    J J Tsai-Wu
    Department of Biological Chemistry, School of Medicine, University of Maryland, Baltimore 21201
    J Bacteriol 173:1902-10. 1991
    ..Furthermore, our results suggest that micA is the same gene as the closely mapped mutY, which encodes the A/G mismatch-specific glycosylase.
  50. pmc Intact MutY and its catalytic domain differentially contact with A/8-oxoG-containing DNA
    X Li
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Nucleic Acids Res 28:4593-603. 2000
    Escherichia coli MutY is an adenine and a weak guanine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, A/C or G/8-oxoG mismatches...
  51. ncbi Potential double-flipping mechanism by E. coli MutY
    P G House
    Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1071, USA
    Prog Nucleic Acid Res Mol Biol 68:349-64. 2001
    ..of the recognition and removal of specific mismatched bases in double-stranded DNAs by the DNA repair glycosylase MutY, a series of structural and functional analyses have been conducted...
  52. ncbi Comparative analysis of 8-oxoG:C, 8-oxoG:A, A:C and C:C DNA repair in extracts from wild type or 8-oxoG DNA glycosylase deficient mammalian and bacterial cells
    Francoise Dantzer
    Centre for Molecular Biology and Neuroscience, and Institute of Medical Microbiology, University of Oslo, Rikshospitalet, 0027 Oslo, Norway
    DNA Repair (Amst) 2:707-18. 2003
    ..coli showed a 50% reduction in the repair of 8-oxoG:C in fpg extracts and an increase to 50% above wild type in mutY. These results show that the mouse OGG1 is the major enzyme for 8-oxoG repair in the MEF cells and does not ..
  53. ncbi Adenine release is fast in MutY-catalyzed hydrolysis of G:A and 8-Oxo-G:A DNA mismatches
    Joe A B McCann
    Department of Biochemistry, McMaster University, Hamilton, Ontario L8S 4M1, Canada
    J Biol Chem 278:29587-92. 2003
    b>MutY, a DNA repair enzyme, is unusual in that it binds exceedingly tightly to its products after the chemical steps of catalysis...
  54. ncbi Characterization of an Escherichia coli mutant MutY with a cysteine to alanine mutation at the iron-sulfur cluster domain
    A Lien Lu
    Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, Maryland 21201, USA
    Biochemistry 42:3742-50. 2003
    Escherichia coli MutY is an adenine and a weak guanine DNA glycosylase involved in reducing mutagenic effects of 7,8-dihydro-8-oxoguanine (8-oxoG)...
  55. ncbi Escherichia coli MutY and Fpg utilize a processive mechanism for target location
    Anthony W Francis
    Department of Chemistry, University of Utah, 315 S 1400 E, Salt Lake City, Utah 84112, USA
    Biochemistry 42:801-10. 2003
    b>MutY and formamidopyrimidine-DNA-glycosylase (Fpg) are base-excision repair (BER) enzymes involved in the 8-oxoguanine repair pathway in Escherichia coli...
  56. ncbi Interactions among the Escherichia coli mutT, mutM, and mutY damage prevention pathways
    Robert G Fowler
    Department of Biological Sciences, San Jose State University, San Jose, CA 95192, USA
    DNA Repair (Amst) 2:159-73. 2003
    We have investigated in detail the interactions between the Escherichia coli mutT, mutM, and mutY error-prevention systems...
  57. pmc Enrichment and elimination of mutY mutators in Escherichia coli populations
    Lucinda Notley-McRobb
    School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia
    Genetics 162:1055-62. 2002
    ..The increase in mutation rates was due to sweeps by mutY mutators...
  58. ncbi DNA-mediated charge transport as a probe of MutY/DNA interaction
    Elizabeth M Boon
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Biochemistry 41:8464-70. 2002
    b>MutY is an Escherichia coli DNA repair enzyme that binds to 8-oxo-G:A and G:A mismatches and catalyzes the deglycosylation of the mismatched 2'-deoxyadenosine...
  59. ncbi Characterization of 2-hydroxyadenine DNA glycosylase activity of Escherichia coli MutY protein
    K Hashiguchi
    Laboratory for Radiation Biology, Graduate School of Science, Kyoto University, Kitashirakawa Oiwakecho, Sakyoku, Kyoto 606 8502, Japan
    Int J Radiat Biol 78:585-92. 2002
    ..Recent studies have shown that 2-ohA is removed from DNA by a human MutY homolog, MYH protein, in vitro...
  60. pmc Regulation of mutY and nature of mutator mutations in Escherichia coli populations under nutrient limitation
    Lucinda Notley-McRobb
    Department of Microbiology G08, University of Sydney, New South Wales 2006, Australia
    J Bacteriol 184:739-45. 2002
    ..One possible explanation for the preponderance of these mutations was that nutrient limitation repressed MutY-dependent DNA repair, resulting in increased proportions of G:C-->T:A transversions...
  61. ncbi Noncysteinyl coordination to the [4Fe-4S]2+ cluster of the DNA repair adenine glycosylase MutY introduced via site-directed mutagenesis. Structural characterization of an unusual histidinyl-coordinated cluster
    Troy E Messick
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 41:3931-42. 2002
    The Escherichia coli DNA repair enzyme MutY plays an important role in the recognition and repair of 7,8-dihydro-8-oxo-2'-deoxyguanosine-2'-deoxyadenosine (OG*A) mismatches in DNA...
  62. pmc Escherichia coli MutY protein has both N-glycosylase and apurinic/apyrimidinic endonuclease activities on A.C and A.G mispairs
    J J Tsai-Wu
    Department of Biological Chemistry, School of Medicine, University of Maryland, Baltimore 21201
    Proc Natl Acad Sci U S A 89:8779-83. 1992
    In Escherichia coli the mutY (or micA)-dependent DNA mismatch repair pathway can convert A degrees G and A degrees C mismatches to C.G and G.C base pairs, respectively, through a short repair-tract mechanism...
  63. ncbi Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates
    Mary Ann Pope
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84103, USA
    J Biol Chem 277:22605-15. 2002
    The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG)...
  64. ncbi Flipping duplex DNA inside out: a double base-flipping reaction mechanism by Escherichia coli MutY adenine glycosylase
    Andrew S Bernards
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA
    J Biol Chem 277:20960-4. 2002
    The Escherichia coli MutY adenine glycosylase plays a critical role in repairing mismatches in DNA between adenine and the oxidatively damaged guanine base 8-oxoguanine...
  65. ncbi MutY catalytic core, mutant and bound adenine structures define specificity for DNA repair enzyme superfamily
    Y Guan
    Department of Molecular Biology, Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Struct Biol 5:1058-64. 1998
    The DNA glycosylase MutY, which is a member of the Helix-hairpin-Helix (HhH) DNA glycosylase superfamily, excises adenine from mispairs with 8-oxoguanine and guanine...
  66. pmc Selection for high mutation rates in chemostats
    E C Cox
    Genetics 77:169-84. 1974
    ..Fitness values vary between chemostats and are strongly correlated (P < 0.001) with fluctuations in population density...
  67. ncbi MutY is down-regulated by oxidative stress in E. coli
    Sun Hee Yoon
    Department of Pharmacology, Seoul National University College of Medicine, 28 Yongon Dong, Chongno Gu, Seoul 110 799, South Korea
    Free Radic Res 37:873-9. 2003
    In Escherichia coli, MutM (8-oxoG DNA glycosylase/lyase or Fpg protein), MutY (adenine DNA glycosylase) and MutT (8-oxodGTPase) function cooperatively to prevent mutation due to 7, 8-dihydro-8-oxoguanine (8-oxoG), a highly mutagenic ..
  68. ncbi Probing the requirements for recognition and catalysis in Fpg and MutY with nonpolar adenine isosteres
    Anthony W Francis
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah, 84112, USA
    J Am Chem Soc 125:16235-42. 2003
    The Escherichia coli DNA repair enzymes Fpg and MutY are involved in the prevention of mutations resulting from 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA...

Research Grants37

  1. REPAIR OF OXIDATIVELY DAMAGED GUANINES IN HUMAN
    A Lien Lu Chang; Fiscal Year: 2007
    ..Thus, repair ol A/8-oxoG and C/8-oxoG by hMYH (MutY adenine glycosylase homolog), hOGG1 (an 8-oxoG glycosylase), and hMSH2/hMSH6 dependent mismatch repair pathways ..
  2. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 2001
    ..The effects of overexpression of SpMYH in E. coli and yeast will be investigated. Yeast mutants defective in myh will be constructed and their mutation rates will be measured. ..
  3. REPAIR OF OXIDATIVELY DAMAGED GUANINES IN HUMAN
    A Lien Lu Chang; Fiscal Year: 2002
    ..The human MutY homolog (hMYH) mismatch repair pathway for repairing A/G, A/C, and A/8-oxoG mismatches will be our major focus...
  4. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 2006
    ..b>MutY and MutY homolog (MYH) adenine glycosylases remove misincorporated adenines paired with template 8-oxoG and reduce ..
  5. Repair of Oxidatively Damaged Guanines
    A LIEN L LU CHANG; Fiscal Year: 2010
    ..We focus on the role of human MutY homolog (hMYH) glycosylase and its interactions with cell cycle and aging regulators...
  6. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 1993
    ..coli and eukaryotes for repairing A/G and/or A/C mismatches will be our major focus. The E. coli MutY protein, involved in this pathway, is an iron-sulfur protein that has been shown to have both DNA glycosylase and 3'..
  7. MECHANISTIC STUDIES OF DNA MISMATCH REPAIR
    A Lien Lu Chang; Fiscal Year: 1992
    ..Because mutagenesis and carcinogenesis are closely related, the knowledge of genes which control mutagenesis will benefit cancer research...
  8. Repair of Oxidatively Damaged Guanines
    A LIEN L LU CHANG; Fiscal Year: 2010
    ..We focus on the role of human MutY homolog (hMYH) glycosylase and its interactions with cell cycle and aging regulators...
  9. Pathway of Spontaneous Mutagenesis in Escherichia coli
    Robert Fowler; Fiscal Year: 2004
    ..Humans possess a number of these specialized DNA polymerases whose activities may be responsible for a significant portion of the human mutational load. ..