mukB

Summary

Gene Symbol: mukB
Description: chromosome condensin MukBEF, ATPase and DNA-binding subunit
Alias: ECK0915, JW0907
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Adachi S, Hiraga S. Mutants suppressing novobiocin hypersensitivity of a mukB null mutation. J Bacteriol. 2003;185:3690-5 pubmed
    The mukB gene is essential for the partitioning of sister chromosomes in Escherichia coli. A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin...
  2. Sun Q, Margolin W. Effects of perturbing nucleoid structure on nucleoid occlusion-mediated toporegulation of FtsZ ring assembly. J Bacteriol. 2004;186:3951-9 pubmed
    ..Previous studies have indicated that chromosome packing plays a role in NO, as mukB mutants grown in rich medium often exhibit FtsZ rings on top of diffuse, unsegregated nucleoids...
  3. Zhuang X. Molecular biology. Unraveling DNA condensation with optical tweezers. Science. 2004;305:188-90 pubmed
  4. Niki H, Imamura R, Kitaoka M, Yamanaka K, Ogura T, Hiraga S. E.coli MukB protein involved in chromosome partition forms a homodimer with a rod-and-hinge structure having DNA binding and ATP/GTP binding activities. EMBO J. 1992;11:5101-9 pubmed
    b>mukB mutants of Escherichia coli are defective in the correct partitioning of replicated chromosomes. This results in the appearance of normal-sized anucleate (chromosome-less) cells during cell proliferation...
  5. Li Y, Stewart N, Berger A, Vos S, Schoeffler A, Berger J, et al. Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction. Proc Natl Acad Sci U S A. 2010;107:18832-7 pubmed publisher
    ..In Escherichia coli, the MukB/E/F complex and topoisomerase IV (Topo IV) are both crucial players in this process...
  6. Weitao T, DasGupta S, Nordstrom K. Role of the mukB gene in chromosome and plasmid partition in Escherichia coli. Mol Microbiol. 2000;38:392-400 pubmed
    ..The locations of oriC and oriR1 (from a Par+ R1 plasmid) were unique and different in the wild-type host. In a mukB mutant, the positions were perturbed for both origins...
  7. Woo J, Lim J, Shin H, Suh M, Ku B, Lee K, et al. Structural studies of a bacterial condensin complex reveal ATP-dependent disruption of intersubunit interactions. Cell. 2009;136:85-96 pubmed publisher
    ..the bacterial MukBEF condensin complex consists of an SMC family protein dimer containing two ATPase head domains, MukB, and two interacting subunits, MukE and MukF...
  8. Yamanaka K, Mitani T, Feng J, Ogura T, Niki H, Hiraga S. Two mutant alleles of mukB, a gene essential for chromosome partition in Escherichia coli. FEMS Microbiol Lett. 1994;123:27-31 pubmed
    The MukB protein is essential for chromosome partitioning in Escherichia coli and consists of 1484 amino acid residues (170 kDa)...
  9. Hayama R, Bahng S, Karasu M, Marians K. The MukB-ParC interaction affects the intramolecular, not intermolecular, activities of topoisomerase IV. J Biol Chem. 2013;288:7653-61 pubmed publisher
    ..We found that the interaction between MukB, the bacterial condensin, and ParC, a subunit of topoisomerase IV, enhanced relaxation of negatively supercoiled ..

More Information

Publications75

  1. Petrushenko Z, Lai C, Rai R, Rybenkov V. DNA reshaping by MukB. Right-handed knotting, left-handed supercoiling. J Biol Chem. 2006;281:4606-15 pubmed
    b>MukB is a bacterial SMC (structural maintenance of chromosome) protein required for faithful chromosome segregation in Escherichia coli...
  2. Danilova O, Reyes Lamothe R, Pinskaya M, Sherratt D, Possoz C. MukB colocalizes with the oriC region and is required for organization of the two Escherichia coli chromosome arms into separate cell halves. Mol Microbiol. 2007;65:1485-92 pubmed
    ..We show that in mukB mutant cells, the two chromosome arms do not separate into distinct cell halves, but extend from pole to pole with ..
  3. Den Blaauwen T, Lindqvist A, Lowe J, Nanninga N. Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)-like protein MukB in the cell. Mol Microbiol. 2001;42:1179-88 pubmed
    Fluorescent polyclonal antibodies specific for MukB have been used to study its localization in Escherichia coli. In wild-type cells, the MukB protein appeared as a limited number of oblong shapes embracing the nucleoid...
  4. Li Y, Schoeffler A, Berger J, Oakley M. The crystal structure of the hinge domain of the Escherichia coli structural maintenance of chromosomes protein MukB. J Mol Biol. 2010;395:11-9 pubmed publisher
    b>MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosomal segregation and condensation in gamma-proteobacteria. MukB and canonical SMC proteins share a characteristic five-domain structure...
  5. Hiraga S, Niki H, Imamura R, Ogura T, Yamanaka K, Feng J, et al. Mutants defective in chromosome partitioning in E. coli. Res Microbiol. 1991;142:189-94 pubmed
    ..The mukA gene is identical to the tolC gene encoding an outer membrane protein. The mukB gene codes for a 177-kDa protein...
  6. Badrinarayanan A, Reyes Lamothe R, Uphoff S, Leake M, Sherratt D. In vivo architecture and action of bacterial structural maintenance of chromosome proteins. Science. 2012;338:528-31 pubmed publisher
    ..Thus, by functioning in pairs, MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors. ..
  7. Vos S, Stewart N, Oakley M, Berger J. Structural basis for the MukB-topoisomerase IV interaction and its functional implications in vivo. EMBO J. 2013;32:2950-62 pubmed publisher
    Chromosome partitioning in Escherichia coli is assisted by two interacting proteins, topoisomerase (topo) IV and MukB. MukB stimulates the relaxation of negative supercoils by topo IV; to understand the mechanism of their action and to ..
  8. Yamanaka K, Ogura T, Niki H, Hiraga S. Identification of two new genes, mukE and mukF, involved in chromosome partitioning in Escherichia coli. Mol Gen Genet. 1996;250:241-51 pubmed
    We have previously reported that the MukB protein is essential for chromosome partitioning in Escherichia coli and that mukB mutants produce anucleate cells and are temperature-sensitive for colony formation...
  9. Saleh A, Yamanaka K, Niki H, Ogura T, Yamazoe M, Hiraga S. Carboxyl terminal region of the MukB protein in Escherichia coli is essential for DNA binding activity. FEMS Microbiol Lett. 1996;143:211-6 pubmed
    The purified MukB protein of Escherichia coli has DNA binding activity and nucleotide binding activity. We have isolated a mutation, mukB1013, causing a substitution of valine at position 1379 to leucine...
  10. Zhou P, Bogan J, Welch K, Pickett S, Wang H, Zaritsky A, et al. Gene transcription and chromosome replication in Escherichia coli. J Bacteriol. 1997;179:163-9 pubmed
    ..Levels of transcripts from four of the genes, dam, nrdA, mukB, and seqA, were reduced at a certain stage during chromosome replication...
  11. Ohsumi K, Yamazoe M, Hiraga S. Different localization of SeqA-bound nascent DNA clusters and MukF-MukE-MukB complex in Escherichia coli cells. Mol Microbiol. 2001;40:835-45 pubmed
    MukF, MukE and MukB proteins form a complex that may participate in the organization of folded sister chromosomes in Escherichia coli...
  12. Hayama R, Marians K. Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Proc Natl Acad Sci U S A. 2010;107:18826-31 pubmed publisher
    ..In Escherichia coli, MukB, a structural maintenance of chromosome-like component of the bacterial condensin, and topoisomerase IV (Topo IV), ..
  13. Sawitzke J, Austin S. Suppression of chromosome segregation defects of Escherichia coli muk mutants by mutations in topoisomerase I. Proc Natl Acad Sci U S A. 2000;97:1671-6 pubmed
    ..suppressed temperature sensitivity and anucleate cell production of cells containing null or point mutations in mukB and null mutations in mukE or mukF...
  14. Badrinarayanan A, Lesterlin C, Reyes Lamothe R, Sherratt D. The Escherichia coli SMC complex, MukBEF, shapes nucleoid organization independently of DNA replication. J Bacteriol. 2012;194:4669-76 pubmed publisher
  15. Niki H, Jaffe A, Imamura R, Ogura T, Hiraga S. The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli. EMBO J. 1991;10:183-93 pubmed
    ..The mutant is defective in a previously undescribed gene, named mukB, located at 21 min on the chromosome. The mukB gene codes for a large protein (approximately 180 kd)...
  16. Chen N, Zinchenko A, Yoshikawa Y, Araki S, Adachi S, Yamazoe M, et al. ATP-induced shrinkage of DNA with MukB protein and the MukBEF complex of Escherichia coli. J Bacteriol. 2008;190:3731-7 pubmed publisher
    ..of individual T4 DNA molecules revealed that the MukBEF complex (bacterial condensin) and its subunit, the MukB (a member of the SMC [structural maintenance of chromosomes] superfamily) homodimer, of Escherichia coli markedly ..
  17. Soppa J. Prokaryotic structural maintenance of chromosomes (SMC) proteins: distribution, phylogeny, and comparison with MukBs and additional prokaryotic and eukaryotic coiled-coil proteins. Gene. 2001;278:253-64 pubmed
    ..coli. A phylogenetic analysis was performed including the prokaryotic coiled-coil proteins as well as SMCs and Rad18 proteins from selected eukaryotes. ..
  18. Adachi S, Kohiyama M, Onogi T, Hiraga S. Localization of replication forks in wild-type and mukB mutant cells of Escherichia coli. Mol Genet Genomics. 2005;274:264-71 pubmed
    ..The formation of BrdU foci was dependent upon ongoing chromosomal replication. A mutant lacking MukB, which is required for proper partitioning of sister chromosomes, failed to maintain the ordered localization of ..
  19. Adachi S, Murakawa Y, Hiraga S. SecA defects are accompanied by dysregulation of MukB, DNA gyrase, chromosome partitioning and DNA superhelicity in Escherichia coli. Microbiology. 2014;160:1648-58 pubmed publisher
    ..component of the membrane protein translocation channel and acyl carrier protein P (AcpP), which binds to SecA and MukB, a functional homologue of structural maintenance of chromosomes protein (SMC), resulted in a defect in chromosome ..
  20. Weitao T, Nordstrom K, DasGupta S. Mutual suppression of mukB and seqA phenotypes might arise from their opposing influences on the Escherichia coli nucleoid structure. Mol Microbiol. 1999;34:157-68 pubmed
    A strain of Escherichia coli in which both the seqA and mukB genes were inactivated displayed partial suppressions of their individual phenotypes...
  21. Yamanaka K, Ogura T, Niki H, Hiraga S. Characterization of the smtA gene encoding an S-adenosylmethionine-dependent methyltransferase of Escherichia coli. FEMS Microbiol Lett. 1995;133:59-63 pubmed
    The mukB operon is located at 21 min on the Escherichia coli chromosome and seems to consist of four genes, orf30 (smtA), mukF, mukE, and mukB...
  22. Yamanaka K, Mitani T, Ogura T, Niki H, Hiraga S. Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli. Mol Microbiol. 1994;13:301-12 pubmed
    The mukB gene codes for a 177 kDa protein, which might be a candidate for a force-generating enzyme in chromosome positioning in Escherichia coli...
  23. Yamanaka K, Ogura T, Niki H, Hiraga S. Identification and characterization of the smbA gene, a suppressor of the mukB null mutant of Escherichia coli. J Bacteriol. 1992;174:7517-26 pubmed
    The mukB gene encodes a protein involved in chromosome partitioning in Escherichia coli...
  24. Luijsterburg M, Noom M, Wuite G, Dame R. The architectural role of nucleoid-associated proteins in the organization of bacterial chromatin: a molecular perspective. J Struct Biol. 2006;156:262-72 pubmed
    ..In this review, we provide an overview of the major nucleoid-associated proteins from a structural perspective and we discuss their possible roles in dynamically shaping the bacterial nucleoid. ..
  25. Yu X, Sun Q, Margolin W. FtsZ rings in mukB mutants with or without the Min system. Biochimie. 2001;83:125-9 pubmed
    ..b>MukB protein is probably involved in nucleoid condensation, and in the absence of MukB, the negative effect of the ..
  26. Jaffe A, Vinella D, D Ari R. The Escherichia coli histone-like protein HU affects DNA initiation, chromosome partitioning via MukB, and cell division via MinCDE. J Bacteriol. 1997;179:3494-9 pubmed
    ..of the characterization of hupA hupB mutants, we observed that the simultaneous absence of the HU2 subunit and the MukB protein, implicated in chromosome partitioning, is lethal for the bacteria; the integrity of either HU or MukB thus ..
  27. Cui Y, Petrushenko Z, Rybenkov V. MukB acts as a macromolecular clamp in DNA condensation. Nat Struct Mol Biol. 2008;15:411-8 pubmed publisher
    ..Using magnetic tweezers, we show that bacterial condensin MukB acts as an ATP-modulated macromolecular assemblage in DNA condensation...
  28. Gullbrand B, Nordstrom K. FtsZ ring formation without subsequent cell division after replication runout in Escherichia coli. Mol Microbiol. 2000;36:1349-59 pubmed
    ..Disruption of the mukB gene caused a significant increase in the region occupied by DNA after the replication runout, consistent with a ..
  29. Lockhart A, Kendrick Jones J. Interaction of the N-terminal domain of MukB with the bacterial tubulin homologue FtsZ. FEBS Lett. 1998;430:278-82 pubmed
    The MukB protein is involved in the process of chromosome partition in Escherichia coli and has a domain structure reminiscent of the eukaryotic motor proteins kinesin and myosin...
  30. Shin H, Lim J, Woo J, Oh B. Focal localization of MukBEF condensin on the chromosome requires the flexible linker region of MukF. FEBS J. 2009;276:5101-10 pubmed publisher
    Condensin complexes are the key mediators of chromosome condensation. The MukB-MukE-MukF complex is a bacterial condensin, in which the MukB subunit forms a V-shaped dimeric structure with two ATPase head domains...
  31. Gully D, Moinier D, Loiseau L, Bouveret E. New partners of acyl carrier protein detected in Escherichia coli by tandem affinity purification. FEBS Lett. 2003;548:90-6 pubmed
    ..Besides the identification of several known partners of ACP, we rediscovered ACP/MukB and ACP/IscS interactions already detected but previously disregarded as due to contamination...
  32. DasGupta S, Maisnier Patin S, Nordstrom K. New genes with old modus operandi. The connection between supercoiling and partitioning of DNA in Escherichia coli. EMBO Rep. 2000;1:323-7 pubmed
    ..are recondensed and moved apart through the concerted activities of topoisomerases and the SeqA (sequestration) and MukB (chromosome condensation) proteins, all of which modulate nucleoid superhelicity...
  33. Gloyd M, Ghirlando R, Guarné A. The role of MukE in assembling a functional MukBEF complex. J Mol Biol. 2011;412:578-90 pubmed publisher
    The MukB-MukE-MukF protein complex is essential for chromosome condensation and segregation in Escherichia coli...
  34. Hu K, Liu E, Dean K, Gingras M, DeGraff W, Trun N. Overproduction of three genes leads to camphor resistance and chromosome condensation in Escherichia coli. Genetics. 1996;143:1521-32 pubmed
    ..present in high copy confer camphor resistance on a cell and suppress mutations in the chromosomal partition gene mukB. Both phenotypes require the same genes...
  35. Nordstrom K, DasGupta S. Partitioning of the Escherichia coli chromosome: superhelicity and condensation. Biochimie. 2001;83:41-8 pubmed
    ..E. coli strains, lacking mukB or seqA functions, are defective in segregation and cell division...
  36. Onogi T, Yamazoe M, Ichinose C, Niki H, Hiraga S. Null mutation of the dam or seqA gene suppresses temperature-sensitive lethality but not hypersensitivity to novobiocin of muk null mutants. J Bacteriol. 2000;182:5898-901 pubmed
    Escherichia coli mukF, mukE, and mukB null mutants have common phenotypes such as temperature-dependent colony formation, anucleate cell production, chromosome cutting by septum closure, and abnormal localization of SeqA-DNA clusters...
  37. Weitao T, Nordstrom K, DasGupta S. Escherichia coli cell cycle control genes affect chromosome superhelicity. EMBO Rep. 2000;1:494-9 pubmed
    ..the negative supercoiling of Escherichia coli chromosome caused by mutations inactivating the cell cycle functions mukB and seqA...
  38. Nolivos S, Upton A, Badrinarayanan A, Müller J, Zawadzka K, Wiktor J, et al. MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation. Nat Commun. 2016;7:10466 pubmed publisher
    ..Displacement of MukBEF is impaired when MukB ATP hydrolysis is compromised and when MatP is absent, leading to a stable association of ter and MukBEF...
  39. Kumar R, Nurse P, Bahng S, Lee C, Marians K. The MukB-topoisomerase IV interaction is required for proper chromosome compaction. J Biol Chem. 2017;292:16921-16932 pubmed publisher
    The bacterial condensin MukB and the cellular decatenating enzyme topoisomerase IV interact...
  40. Dame R. The role of nucleoid-associated proteins in the organization and compaction of bacterial chromatin. Mol Microbiol. 2005;56:858-70 pubmed
    ..Many of these new insights can be attributed to the use of recently developed biophysical techniques. ..
  41. Kido M, Yamanaka K, Mitani T, Niki H, Ogura T, Hiraga S. RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli. J Bacteriol. 1996;178:3917-25 pubmed
    ..isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation...
  42. Donachie W. Co-ordinate regulation of the Escherichia coli cell cycle or The cloud of unknowing. Mol Microbiol. 2001;40:779-85 pubmed
    ..A discussion of some aspects of the regulation of chromosome replication, segregation and cell division in Escherichia coli. ..
  43. Hiraga S. Chromosome and plasmid partition in Escherichia coli. Annu Rev Biochem. 1992;61:283-306 pubmed
  44. Ezaki B, Ogura T, Niki H, Hiraga S. Partitioning of a mini-F plasmid into anucleate cells of the mukB null mutant. J Bacteriol. 1991;173:6643-6 pubmed
    The partition-proficient mini-F plasmid pXX325 was stably maintained in the mukB null mutant, which is defective in chromosome partitioning into the two daughter cells...
  45. Feng J, Yamanaka K, Niki H, Ogura T, Hiraga S. New killing system controlled by two genes located immediately upstream of the mukB gene in Escherichia coli. Mol Gen Genet. 1994;243:136-47 pubmed
    The nucleotide sequence was determined of the region upstream of the mukB gene of Escherichia coli. Two new genes were found, designated kicA and kicB (killing of cell); the gene order is kicB-kicA-mukB...
  46. Ewart C, Jude D, Thain J, Nichols W. Frequency and mechanism of resistance to antibacterial action of ZM 240401, (6S)-6-fluoro-shikimic acid. Antimicrob Agents Chemother. 1995;39:87-93 pubmed
    ..We propose that the loss of susceptibility to (6S)-6-fluoro-shikimic acid in spontaneous resistant variants is due to the loss of activity of the transport system by which it enters the bacterial cytoplasm. ..
  47. Graumann P, Knust T. Dynamics of the bacterial SMC complex and SMC-like proteins involved in DNA repair. Chromosome Res. 2009;17:265-75 pubmed publisher
    ..The bacterial SMC (MukB) complex appears to perform a condensin-like function, while SbcC and RecN act early during DNA repair, but ..
  48. Gully D, Bouveret E. A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli. Proteomics. 2006;6:282-93 pubmed
    ..Taken together, these results describe an integrated protein network that could be involved in the coordination of phospholipid metabolism. ..
  49. Sun Q, Yu X, Margolin W. Assembly of the FtsZ ring at the central division site in the absence of the chromosome. Mol Microbiol. 1998;29:491-503 pubmed
    ..by chromosome positioning and structure in Escherichia coli, we investigated its localization in parC and mukB mutants that are defective for chromosome segregation...
  50. Schleiffer A, Kaitna S, Maurer Stroh S, Glotzer M, Nasmyth K, Eisenhaber F. Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners. Mol Cell. 2003;11:571-5 pubmed
    ..Scc1 interacts with SMC proteins through N- and C-terminal domains to form a ring-like structure. Since these are the only domains conserved among kleisins, we suggest that ring formation with SMC proteins may define this family. ..
  51. Petrushenko Z, Lai C, Rybenkov V. Antagonistic interactions of kleisins and DNA with bacterial Condensin MukB. J Biol Chem. 2006;281:34208-17 pubmed
    ..The SMC subunit of the complex, MukB, promotes DNA condensation in vitro and in vivo; however, all three subunits are required for the function of ..
  52. Matoba K, Yamazoe M, Mayanagi K, Morikawa K, Hiraga S. Comparison of MukB homodimer versus MukBEF complex molecular architectures by electron microscopy reveals a higher-order multimerization. Biochem Biophys Res Commun. 2005;333:694-702 pubmed
    The complex of MukF, MukE, and MukB proteins participates in organization of sister chromosomes and partitioning into both daughter cells in Escherichia coli...
  53. Nicolas E, Upton A, Uphoff S, Henry O, Badrinarayanan A, Sherratt D. The SMC complex MukBEF recruits topoisomerase IV to the origin of replication region in live Escherichia coli. MBio. 2014;5:e01001-13 pubmed publisher
    ..SMC) complex, MukBEF, and topoisomerase IV (TopoIV) interact in vitro through a direct contact between the MukB dimerization hinge and the C-terminal domain of ParC, the catalytic subunit of TopoIV...
  54. Li Y, Weitzel C, Arnold R, Oakley M. Identification of interacting regions within the coiled coil of the Escherichia coli structural maintenance of chromosomes protein MukB. J Mol Biol. 2009;391:57-73 pubmed publisher
    b>MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosome segregation and condensation in Escherichia coli and other gamma-proteobacteria...
  55. Melby T, Ciampaglio C, Briscoe G, Erickson H. The symmetrical structure of structural maintenance of chromosomes (SMC) and MukB proteins: long, antiparallel coiled coils, folded at a flexible hinge. J Cell Biol. 1998;142:1595-604 pubmed
    ..Here, we have visualized by EM the SMC protein from Bacillus subtilis (BsSMC) and MukB from Escherichia coli, which we argue is a divergent SMC protein...
  56. She W, Wang Q, Mordukhova E, Rybenkov V. MukEF Is required for stable association of MukB with the chromosome. J Bacteriol. 2007;189:7062-8 pubmed
    b>MukB is a bacterial SMC(structural maintenance of chromosome) protein required for correct folding of the Escherichia coli chromosome. MukB acts in complex with the two non-SMC proteins, MukE and MukF. The role of MukEF is unclear...
  57. Champion K, Higgins N. Growth rate toxicity phenotypes and homeostatic supercoil control differentiate Escherichia coli from Salmonella enterica serovar Typhimurium. J Bacteriol. 2007;189:5839-49 pubmed
  58. Vinella D, D Ari R. Overview of controls in the Escherichia coli cell cycle. Bioessays. 1995;17:527-36 pubmed
    ..The replication initiator is the DnaA protein. In nucleoid segregation, the MukB protein, required for proper partitioning, may be a member of the myosin-kinesin superfamily of mechanoenzymes...
  59. Beese L, Steitz T. Structural basis for the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism. EMBO J. 1991;10:25-33 pubmed
    ..9 A from the first. Virtually all aspects of the pretransition state substrate complex are directly seen in the structures, and only very small changes in the positions of phosphate atoms are required to form the transition state. ..
  60. Fennell Fezzie R, Gradia S, Akey D, Berger J. The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins. EMBO J. 2005;24:1921-30 pubmed
    The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA...
  61. Bahng S, Hayama R, Marians K. MukB-mediated Catenation of DNA Is ATP and MukEF Independent. J Biol Chem. 2016;291:23999-24008 pubmed
    ..The Escherichia coli condesin is MukB, a structural maintenance of chromosomes (SMC)-like protein, which forms a complex with MukE and the kleisin MukF...
  62. Yamazoe M, Onogi T, Sunako Y, Niki H, Yamanaka K, Ichimura T, et al. Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli. EMBO J. 1999;18:5873-84 pubmed
    mukF, mukE and mukB genes are essential for the process of chromosome partitioning in Escherichia coli...
  63. Casaregola S, Norris V, Goldberg M, Holland I. Identification of a 180 kD protein in Escherichia coli related to a yeast heavy-chain myosin. Mol Microbiol. 1990;4:505-11 pubmed
    ..coli raises the possibility that such a protein might generate the force required for movement in processes such as nucleoid segregation and cell division. ..
  64. Adachi S, Fukushima T, Hiraga S. Dynamic events of sister chromosomes in the cell cycle of Escherichia coli. Genes Cells. 2008;13:181-97 pubmed publisher
    ..Cohesion phenomenon of oriC sister copies was also observed in a mukB null mutant cells under some growth conditions...
  65. Kruse T, Møller Jensen J, Løbner Olesen A, Gerdes K. Dysfunctional MreB inhibits chromosome segregation in Escherichia coli. EMBO J. 2003;22:5283-92 pubmed
    ..Our observations indicate that MreB filaments participate in directional chromosome movement and segregation. ..
  66. Wang Q, Mordukhova E, Edwards A, Rybenkov V. Chromosome condensation in the absence of the non-SMC subunits of MukBEF. J Bacteriol. 2006;188:4431-41 pubmed
    ..The overproduction of MukB leads to a similar chromosome condensation, even in the absence of MukE and MukF...