Gene Symbol: mtn
Description: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Alias: ECK0158, JW0155, mtnN, pfs, yadA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Ferro A, Barrett A, Shapiro S. Kinetic properties and the effect of substrate analogues on 5'-methylthioadenosine nucleosidase from Escherichia coli. Biochim Biophys Acta. 1976;438:487-94 pubmed
    ..The Ki values for 5'-ethylthioadenosine, 5'-n-propylthioadenosine, and S-adenosylhomocysteine were determined to be 1.3-10(-7) M, 4.6-10(-8) M, and 1.92-10(-7) M respectively. ..
  2. Singh V, Evans G, Lenz D, Mason J, Clinch K, Mee S, et al. Femtomolar transition state analogue inhibitors of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Escherichia coli. J Biol Chem. 2005;280:18265-73 pubmed
    ..The accompanying article reports crystal structures of MTAN with these analogues. ..
  3. Lee J, Singh V, Evans G, Tyler P, Furneaux R, Cornell K, et al. Structural rationale for the affinity of pico- and femtomolar transition state analogues of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase. J Biol Chem. 2005;280:18274-82 pubmed
    ..Distance analysis of the nucleophile and leaving group show that MT-ImmA is a mimic of an early transition state, while MT-DADMe-ImmA is a better mimic of the highly dissociated transition state of E. coli MTAN. ..
  4. Cadieux N, Bradbeer C, Reeger Schneider E, Koster W, Mohanty A, Wiener M, et al. Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli. J Bacteriol. 2002;184:706-17 pubmed
    ..A null mutation in btuF, but not in the flanking genes pfs and yadS, strongly decreased CN-Cbl utilization and transport into the cytoplasm...
  5. Della Ragione F, Porcelli M, Cartenì Farina M, Zappia V, Pegg A. Escherichia coli S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase. Purification, substrate specificity and mechanism of action. Biochem J. 1985;232:335-41 pubmed
    ..The results obtained allow the hypothesis of a mechanism of enzymic catalysis requiring as a key step the protonation of N-7 of the purine ring. ..
  6. Singh V, Lee J, Nunez S, Howell P, Schramm V. Transition state structure of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Escherichia coli and its similarity to transition state analogues. Biochemistry. 2005;44:11647-59 pubmed
    ..Transition state analogues that resemble this transition state structure are powerful inhibitors, and their molecular electrostatic potential maps closely resemble that of the transition state. ..
  7. Schauder S, Shokat K, Surette M, Bassler B. The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule. Mol Microbiol. 2001;41:463-76 pubmed
    ..This result suggests that, unlike quorum sensing via the family of related homoserine lactone autoinducers, AI-2 is a unique, 'universal' signal that could be used by a variety of bacteria for communication among and between species. ..
  8. Wurgler S, Richardson C. Structure and regulation of the gene for dGTP triphosphohydrolase from Escherichia coli. Proc Natl Acad Sci U S A. 1990;87:2740-4 pubmed
    ..An E. coli strain containing a null allele has no detectable phenotype when grown at 30-42 degrees C in rich medium. A transition of C to T in a potential promoter of dgt is required for expression of the optA1 phenotype. ..
  9. Gutierrez J, Crowder T, Rinaldo Matthis A, Ho M, Almo S, Schramm V. Transition state analogs of 5'-methylthioadenosine nucleosidase disrupt quorum sensing. Nat Chem Biol. 2009;5:251-7 pubmed publisher
    ..These results support MTAN's role in quorum sensing and its potential as a target for bacterial anti-infective drug design. ..

More Information


  1. Lee J, Smith G, Horvatin C, Huang D, Cornell K, Riscoe M, et al. Structural snapshots of MTA/AdoHcy nucleosidase along the reaction coordinate provide insights into enzyme and nucleoside flexibility during catalysis. J Mol Biol. 2005;352:559-74 pubmed
    ..A "catalytic movie" detailing substrate binding, catalysis, and product release is presented. ..
  2. Lee J, Cornell K, Riscoe M, Howell P. Structure of Escherichia coli 5'-methylthioadenosine/ S-adenosylhomocysteine nucleosidase inhibitor complexes provide insight into the conformational changes required for substrate binding and catalysis. J Biol Chem. 2003;278:8761-70 pubmed
    ..and Howell, P. L. (2001) Structure (Camb.) 9, 941-953) structure provide evidence for a ligand-induced conformational change in the active site and the substrate preference of the enzyme. The enzymatic mechanism has been re-examined. ..
  3. Ren D, Bedzyk L, Ye R, Thomas S, Wood T. Differential gene expression shows natural brominated furanones interfere with the autoinducer-2 bacterial signaling system of Escherichia coli. Biotechnol Bioeng. 2004;88:630-42 pubmed
    ..indicated that 100 microg/mL furanone decreased the extracellular concentration of AI-2 2-fold, yet luxS and pfs transcription were not significantly altered...
  4. Allart B, Gatel M, Guillerm D, Guillerm G. The catalytic mechanism of adenosylhomocysteine/methylthioadenosine nucleosidase from Escherichia coli--chemical evidence for a transition state with a substantial oxocarbenium character. Eur J Biochem. 1998;256:155-62 pubmed
    ..This result indicates a transition state with a substantial oxocarbenium character. From these data, the reaction mechanism for AdoHcy/MeSAdo nucleosidase is discussed. ..
  5. Challand M, Ziegert T, Douglas P, Wood R, Kriek M, Shaw N, et al. Product inhibition in the radical S-adenosylmethionine family. FEBS Lett. 2009;583:1358-62 pubmed publisher
    ..Addition of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) to BioB, LipA or ThiH activity assays removed the product inhibition by catalysing the hydrolysis of DOA and gave an increase in activity. ..
  6. Lee J, Luong W, Huang D, Cornell K, Riscoe M, Howell P. Mutational analysis of a nucleosidase involved in quorum-sensing autoinducer-2 biosynthesis. Biochemistry. 2005;44:11049-57 pubmed
    ..Furthermore, mutation of Arg193 to alanine shows that the nucleophilic water is able to direct its attack without assistance from the enzyme. This mutagenesis study has allowed a reevaluation of the catalytic mechanism. ..
  7. Kim Y, Lew C, Gralla J. Escherichia coli pfs transcription: regulation and proposed roles in autoinducer-2 synthesis and purine excretion. J Bacteriol. 2006;188:7457-63 pubmed
    b>Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing...
  8. Cornell K, Riscoe M. Cloning and expression of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: identification of the pfs gene product. Biochim Biophys Acta. 1998;1396:8-14 pubmed
    ..coli (designated pfs) encoded MTA/SAH nucleosidase...
  9. Thomas K, Cameron S, Almo S, Burgos E, Gulab S, Schramm V. Active site and remote contributions to catalysis in methylthioadenosine nucleosidases. Biochemistry. 2015;54:2520-9 pubmed publisher
    ..The overall protein architectures of these closely related proteins are implicated in contributing to the catalytic site differences. ..
  10. Han X, Bai H, Liu L, Chen W, Ding C, Hu Q, et al. [Cloning and expression of luxS and pfs and in vitro biosynthesis autoinducer 2 of avian pathogenic Escherichia coli from Anhui Province]. Wei Sheng Wu Xue Bao. 2012;52:1167-72 pubmed
    ..In this study, the method of biosynthesis of AI-2 was established using recombinant LuxS and Pfs of avian pathogenic Escherichia coli (APEC), which will be benefit for future study of the role of AI-2 in APEC...
  11. Albers E. Metabolic characteristics and importance of the universal methionine salvage pathway recycling methionine from 5'-methylthioadenosine. IUBMB Life. 2009;61:1132-42 pubmed publisher
    ..Thus, promising targets for antimicrobial agents have been identified. Other medical topics to which this pathway has connections are cancer, apoptosis, and inflammatory response. ..
  12. Cornell K, Swarts W, Barry R, Riscoe M. Characterization of recombinant Eschericha coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: analysis of enzymatic activity and substrate specificity. Biochem Biophys Res Commun. 1996;228:724-32 pubmed
    ..The truncated enzyme exhibited a K(m)[MTA] of 1.43 microM, approximately 3 fold higher than the K(m) reported for the full-length nucleosidase. ..
  13. Beeston A, Surette M. pfs-dependent regulation of autoinducer 2 production in Salmonella enterica serovar Typhimurium. J Bacteriol. 2002;184:3450-6 pubmed
    ..G. Surette and B. L. Bassler, Mol. Microbiol. 31:585-595, 1999). In addition to LuxS, the pfs gene product (Pfs) is required for AI-2 production, as well as S-adenosylhomocysteine (SAH) (S. Schauder, K...
  14. Lee J, Cornell K, Riscoe M, Howell P. Expression, purification, crystallization and preliminary X-ray analysis of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase. Acta Crystallogr D Biol Crystallogr. 2001;57:150-2 pubmed
    ..On the basis of density calculations, two monomers are predicted per asymmetric unit (Matthews coefficient, V(M) = 2.37 A(3) Da(-1)), with a solvent content of 48%. ..
  15. Depping R, Lohaus C, Meyer H, RĂ¼ger W. The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: target proteins identified. Biochem Biophys Res Commun. 2005;335:1217-23 pubmed
    ..E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication. ..
  16. Li J, Wang L, Hashimoto Y, Tsao C, Wood T, Valdes J, et al. A stochastic model of Escherichia coli AI-2 quorum signal circuit reveals alternative synthesis pathways. Mol Syst Biol. 2006;2:67 pubmed
    ..testable hypotheses, and made several discoveries: (1) the mRNA transcript and protein levels of AI-2 synthases, Pfs and LuxS, do not contribute to the dramatically increased level of AI-2 found when cells are grown in the presence ..
  17. Lee J, Cornell K, Riscoe M, Howell P. Structure of E. coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase reveals similarity to the purine nucleoside phosphorylases. Structure. 2001;9:941-53 pubmed
    ..The structure of MTA/AdoHcy nucleosidase is the first structure of a prokaryotic nucleoside N-ribohydrolase specific for 6-aminopurines...
  18. Choi Rhee E, Cronan J. A nucleosidase required for in vivo function of the S-adenosyl-L-methionine radical enzyme, biotin synthase. Chem Biol. 2005;12:589-93 pubmed
    ..that Escherichia coli strains lacking the 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase encoded by the pfs gene are deficient in biotin synthase activity due to accumulation of 5'-deoxyadenosine, a new substrate of the pfs-..
  19. Allart B, Guillerm D, Guillerm G. On the catalytic mechanism of adenosylhomocysteine/methylthioadenosine nucleosidase from E. coli. Nucleosides Nucleotides. 1999;18:861-2 pubmed
    ..AdoHcy/MTA nucleosidase has been under scrutiny in a series of studies to explore its catalytic mechanism. ..