mscL

Summary

Gene Symbol: mscL
Description: mechanosensitive channel protein, high conductance
Alias: ECK3277, JW3252, stkA, yhdC
Species: Escherichia coli str. K-12 substr. MG1655
Products:     mscL

Top Publications

  1. Yoshimura K, Batiza A, Schroeder M, Blount P, Kung C. Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity. Biophys J. 1999;77:1960-72 pubmed
    Mechanosensitive channel large (MscL) encodes the large conductance mechanosensitive channel of the Escherichia coli inner membrane that protects bacteria from lysis upon osmotic shock...
  2. Nomura T, Cranfield C, Deplazes E, Owen D, Macmillan A, Battle A, et al. Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS. Proc Natl Acad Sci U S A. 2012;109:8770-5 pubmed publisher
    Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in the protection of bacterial cells against hypoosmotic shock...
  3. Anishkin A, Gendel V, Sharifi N, Chiang C, Shirinian L, Guy H, et al. On the conformation of the COOH-terminal domain of the large mechanosensitive channel MscL. J Gen Physiol. 2003;121:227-44 pubmed
    COOH-terminal (S3) domains are conserved within the MscL family of bacterial mechanosensitive channels, but their function remains unclear...
  4. Ou X, Blount P, Hoffman R, Kung C. One face of a transmembrane helix is crucial in mechanosensitive channel gating. Proc Natl Acad Sci U S A. 1998;95:11471-5 pubmed
    b>MscL is a mechanosensitive channel in bacteria that responds directly to membrane tension by opening a large conductance pore...
  5. Sukharev S, Blount P, Martinac B, Blattner F, Kung C. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature. 1994;368:265-8 pubmed
    ..experiments with Escherichia coli envelope have revealed a mechanosensitive channel with very large conductance (MscL) and one with a smaller conductance (MscS) which may be important in osmoregulation...
  6. Gullingsrud J, Schulten K. Gating of MscL studied by steered molecular dynamics. Biophys J. 2003;85:2087-99 pubmed
    Steered molecular dynamics simulations of the mechanosensitive channel of large conductance, MscL, were used to investigate how forces arising from membrane tension induce gating of the channel...
  7. Gandhi C, Walton T, Rees D. OCAM: a new tool for studying the oligomeric diversity of MscL channels. Protein Sci. 2011;20:313-26 pubmed publisher
    ..We have applied OCAM to study the mechanosensitive channel of large conductance (MscL) and find that these proteins can exist in multiple oligomeric states ranging from tetramers up to possible ..
  8. Levina N, Tötemeyer S, Stokes N, Louis P, Jones M, Booth I. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 1999;18:1730-7 pubmed
    ..Electrophysiological measurements have identified multiple channels in Escherichia coli cells. One gene, mscL, encoding a large conductance channel has previously been described, but null mutants were without well-defined ..
  9. Sukharev S, Betanzos M, Chiang C, Guy H. The gating mechanism of the large mechanosensitive channel MscL. Nature. 2001;409:720-4 pubmed
    The mechanosensitive channel of large conductance, MscL, is a ubiquitous membrane-embedded valve involved in turgor regulation in bacteria...
  10. Corry B, Rigby P, Liu Z, Martinac B. Conformational changes involved in MscL channel gating measured using FRET spectroscopy. Biophys J. 2005;89:L49-51 pubmed
    ..The diameter of the protein is found to increase by 16 A upon channel activation. ..

Detail Information

Publications68

  1. Yoshimura K, Batiza A, Schroeder M, Blount P, Kung C. Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity. Biophys J. 1999;77:1960-72 pubmed
    Mechanosensitive channel large (MscL) encodes the large conductance mechanosensitive channel of the Escherichia coli inner membrane that protects bacteria from lysis upon osmotic shock...
  2. Nomura T, Cranfield C, Deplazes E, Owen D, Macmillan A, Battle A, et al. Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS. Proc Natl Acad Sci U S A. 2012;109:8770-5 pubmed publisher
    Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in the protection of bacterial cells against hypoosmotic shock...
  3. Anishkin A, Gendel V, Sharifi N, Chiang C, Shirinian L, Guy H, et al. On the conformation of the COOH-terminal domain of the large mechanosensitive channel MscL. J Gen Physiol. 2003;121:227-44 pubmed
    COOH-terminal (S3) domains are conserved within the MscL family of bacterial mechanosensitive channels, but their function remains unclear...
  4. Ou X, Blount P, Hoffman R, Kung C. One face of a transmembrane helix is crucial in mechanosensitive channel gating. Proc Natl Acad Sci U S A. 1998;95:11471-5 pubmed
    b>MscL is a mechanosensitive channel in bacteria that responds directly to membrane tension by opening a large conductance pore...
  5. Sukharev S, Blount P, Martinac B, Blattner F, Kung C. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature. 1994;368:265-8 pubmed
    ..experiments with Escherichia coli envelope have revealed a mechanosensitive channel with very large conductance (MscL) and one with a smaller conductance (MscS) which may be important in osmoregulation...
  6. Gullingsrud J, Schulten K. Gating of MscL studied by steered molecular dynamics. Biophys J. 2003;85:2087-99 pubmed
    Steered molecular dynamics simulations of the mechanosensitive channel of large conductance, MscL, were used to investigate how forces arising from membrane tension induce gating of the channel...
  7. Gandhi C, Walton T, Rees D. OCAM: a new tool for studying the oligomeric diversity of MscL channels. Protein Sci. 2011;20:313-26 pubmed publisher
    ..We have applied OCAM to study the mechanosensitive channel of large conductance (MscL) and find that these proteins can exist in multiple oligomeric states ranging from tetramers up to possible ..
  8. Levina N, Tötemeyer S, Stokes N, Louis P, Jones M, Booth I. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 1999;18:1730-7 pubmed
    ..Electrophysiological measurements have identified multiple channels in Escherichia coli cells. One gene, mscL, encoding a large conductance channel has previously been described, but null mutants were without well-defined ..
  9. Sukharev S, Betanzos M, Chiang C, Guy H. The gating mechanism of the large mechanosensitive channel MscL. Nature. 2001;409:720-4 pubmed
    The mechanosensitive channel of large conductance, MscL, is a ubiquitous membrane-embedded valve involved in turgor regulation in bacteria...
  10. Corry B, Rigby P, Liu Z, Martinac B. Conformational changes involved in MscL channel gating measured using FRET spectroscopy. Biophys J. 2005;89:L49-51 pubmed
    ..The diameter of the protein is found to increase by 16 A upon channel activation. ..
  11. Sukharev S, Anishkin A. Mechanosensitive channels: what can we learn from 'simple' model systems?. Trends Neurosci. 2004;27:345-51 pubmed
    ..Here, the conformational transition driven by membrane tension in the bacterial channel MscL is discussed...
  12. Blount P, Sukharev S, Moe P, Nagle S, Kung C. Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria. Biol Cell. 1996;87:1-8 pubmed
    ..A gene called mscL, which was isolated from Escherichia coli, was the first gene shown to encode a mechanosensitive channel activity...
  13. Blount P, Schroeder M, Kung C. Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress. J Biol Chem. 1997;272:32150-7 pubmed
    b>MscL is a channel found in bacterial plasma membranes that opens a large pore in response to mechanical stress...
  14. Moe P, Blount P, Kung C. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol. 1998;28:583-92 pubmed
    b>mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge...
  15. Blount P, Sukharev S, Moe P, Schroeder M, Guy H, Kung C. Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli. EMBO J. 1996;15:4798-805 pubmed
    We have studied the membrane topology and multimeric structure of a mechanosensitive channel, MscL, which we previously isolated and cloned from Escherichia coli...
  16. Perozo E, Rees D. Structure and mechanism in prokaryotic mechanosensitive channels. Curr Opin Struct Biol. 2003;13:432-42 pubmed
    ..The X-ray crystal structures of MscL and MscS offer a unique opportunity to identify the types of protein motions associated with the opening and ..
  17. Sukharev S, Martinac B, Arshavsky V, Kung C. Two types of mechanosensitive channels in the Escherichia coli cell envelope: solubilization and functional reconstitution. Biophys J. 1993;65:177-83 pubmed
  18. Yoshimura K, Batiza A, Kung C. Chemically charging the pore constriction opens the mechanosensitive channel MscL. Biophys J. 2001;80:2198-206 pubmed
    b>MscL is a bacterial mechanosensitive channel that protects the cell from osmotic downshock...
  19. Häse C, Le Dain A, Martinac B. Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli. J Biol Chem. 1995;270:18329-34 pubmed
    The large mechanosensitive ion channel (MscL) of Escherichia coli was expressed on a plasmid encoding MscL as a fusion protein with glutathione S-transferase in an Escherichia coli strain containing a disruption in the chromosomal mscL ..
  20. Sukharev S, Sigurdson W, Kung C, Sachs F. Energetic and spatial parameters for gating of the bacterial large conductance mechanosensitive channel, MscL. J Gen Physiol. 1999;113:525-40 pubmed
    b>MscL is multimeric protein that forms a large conductance mechanosensitive channel in the inner membrane of Escherichia coli...
  21. Chiang C, Anishkin A, Sukharev S. Gating of the large mechanosensitive channel in situ: estimation of the spatial scale of the transition from channel population responses. Biophys J. 2004;86:2846-61 pubmed
    ..Despite increasing evidence for the open-state conformation of MscL, the energetic description of its complex gating remains incomplete...
  22. Liu Z, Gandhi C, Rees D. Structure of a tetrameric MscL in an expanded intermediate state. Nature. 2009;461:120-4 pubmed publisher
    ..In bacteria, mechanosensation is mediated by the mechanosensitive channels of large (MscL), small (MscS), potassium-dependent (MscK) and mini (MscM) conductances...
  23. Betanzos M, Chiang C, Guy H, Sukharev S. A large iris-like expansion of a mechanosensitive channel protein induced by membrane tension. Nat Struct Biol. 2002;9:704-10 pubmed
    b>MscL, a bacterial mechanosensitive channel of large conductance, is the first structurally characterized mechanosensor protein. Molecular models of its gating mechanisms are tested here...
  24. Häse C, Le Dain A, Martinac B. Molecular dissection of the large mechanosensitive ion channel (MscL) of E. coli: mutants with altered channel gating and pressure sensitivity. J Membr Biol. 1997;157:17-25 pubmed
    In the search for the essential functional domains of the large mechanosensitive ion channel (MscL) of E. coli, we have cloned several mutants of the mscL gene into a glutathione S-transferase fusion protein expression system...
  25. Cruickshank C, Minchin R, Le Dain A, Martinac B. Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli. Biophys J. 1997;73:1925-31 pubmed
    The open channel diameter of Escherichia coli recombinant large-conductance mechanosensitive ion channels (MscL) was estimated using the model of Hille (Hille, B. 1968. Pharmacological modifications of the sodium channels of frog nerve. J...
  26. Shapovalov G, Lester H. Gating transitions in bacterial ion channels measured at 3 microns resolution. J Gen Physiol. 2004;124:151-61 pubmed
    ..Two examples, the Escherichia coli mechanosensitive ion channels Ec-MscS and Ec-MscL, pass currents of 125-300 pA...
  27. Pivetti C, Yen M, Miller S, Busch W, Tseng Y, Booth I, et al. Two families of mechanosensitive channel proteins. Microbiol Mol Biol Rev. 2003;67:66-85, table of contents pubmed
    ..Two families of ubiquitous MS channels are recognized, and these have been designated the MscL and MscS families...
  28. Sukharev S, Durell S, Guy H. Structural models of the MscL gating mechanism. Biophys J. 2001;81:917-36 pubmed
    Three-dimensional structural models of the mechanosensitive channel of large conductance, MscL, from the bacteria Mycobacterium tuberculosis and Escherichia coli were developed for closed, intermediate, and open conformations...
  29. Arkin I, Sukharev S, Blount P, Kung C, Brunger A. Helicity, membrane incorporation, orientation and thermal stability of the large conductance mechanosensitive ion channel from E. coli. Biochim Biophys Acta. 1998;1369:131-40 pubmed
    In this report, we present structural studies on the large conductance mechanosensitive ion channel (MscL) from E. coli in detergent micelles and lipid vesicles...
  30. Park K, Berrier C, Martinac B, Ghazi A. Purification and functional reconstitution of N- and C-halves of the MscL channel. Biophys J. 2004;86:2129-36 pubmed
    b>MscL is a mechanosensitive channel gated by membrane tension in the lipid bilayer alone. Its structure, known from x-ray crystallography, indicates that it is a homopentamer...
  31. Sukharev S, Blount P, Martinac B, Kung C. Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities. Annu Rev Physiol. 1997;59:633-57 pubmed
    ..responses are ubiquitous and diverse, the molecular bases of mechanosensation in most cases remain mysterious MscL, a mechanosensitive channel of large conductance of Escherichia coli and its bacterial homologues are the first and ..
  32. Gullingsrud J, Kosztin D, Schulten K. Structural determinants of MscL gating studied by molecular dynamics simulations. Biophys J. 2001;80:2074-81 pubmed
    The mechanosensitive channel of large conductance (MscL) in prokaryotes plays a crucial role in exocytosis as well as in the response to osmotic downshock. The channel can be gated by tension in the membrane bilayer...
  33. Stokes N, Murray H, Subramaniam C, Gourse R, Louis P, Bartlett W, et al. A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS. Proc Natl Acad Sci U S A. 2003;100:15959-64 pubmed
    The mechanosensitive (MS) channels MscS and MscL are essential for the survival of hypoosmotic shock by Escherichia coli cells. We demonstrate that MscS and MscL are induced by osmotic stress and by entry into stationary phase...
  34. Blount P, Sukharev S, Schroeder M, Nagle S, Kung C. Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli. Proc Natl Acad Sci U S A. 1996;93:11652-7 pubmed
    b>MscL is a channel that opens a large pore in the Escherichia coli cytoplasmic membrane in response to mechanical stress...
  35. Gu L, Liu W, Martinac B. Electromechanical coupling model of gating the large mechanosensitive ion channel (MscL) of Escherichia coli by mechanical force. Biophys J. 1998;74:2889-902 pubmed
    ..theoretical electromechanical coupling (EMC) model of gating of the large-conductance mechanosensitive ion channel (MscL)...
  36. Wiggins P, Phillips R. Membrane-protein interactions in mechanosensitive channels. Biophys J. 2005;88:880-902 pubmed
    ..conformation and function with specific reference to the case of the mechanosensitive channel of large conductance (MscL)...
  37. Strop P, Bass R, Rees D. Prokaryotic mechanosensitive channels. Adv Protein Chem. 2003;63:177-209 pubmed
  38. Ajouz B, Berrier C, Garrigues A, Besnard M, Ghazi A. Release of thioredoxin via the mechanosensitive channel MscL during osmotic downshock of Escherichia coli cells. J Biol Chem. 1998;273:26670-4 pubmed
    Escherichia coli cells possess several mechanosensitive ion channels but only MscL, the channel with the highest conductance, which is activated at the highest membrane tension, has been cloned...
  39. Perozo E, Kloda A, Cortes D, Martinac B. Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating. Nat Struct Biol. 2002;9:696-703 pubmed
    ..Structural changes in the Escherichia coli large MS channel (MscL) were studied under nominally zero transbilayer pressures using both patch clamp and EPR spectroscopic approaches...
  40. Rees D, Chang G, Spencer R. Crystallographic analyses of ion channels: lessons and challenges. J Biol Chem. 2000;275:713-6 pubmed
  41. Sukharev S, Schroeder M, McCaslin D. Stoichiometry of the large conductance bacterial mechanosensitive channel of E. coli. A biochemical study. J Membr Biol. 1999;171:183-93 pubmed
    b>MscL, a 15 kDa transmembrane protein, is the only component involved in the formation of a 3 nS channel in the inner membrane of Escherichia coli that opens in response to mechanical or osmotic stress...
  42. Clayton D, Shapovalov G, Maurer J, Dougherty D, Lester H, Kochendoerfer G. Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2004;101:4764-9 pubmed
    ..generate multimilligram quantities of the mechanosensitive channel of large conductance from Escherichia coli (Ec-MscL) and Mycobacterium tuberculosis (Tb-MscL)...
  43. Martinac B. Mechanosensitive channels in prokaryotes. Cell Physiol Biochem. 2001;11:61-76 pubmed
    ..Cloning of MscL and MscS, the MS channels of Large and Small conductance found in Bacteria and elucidation of the 3D crystal ..
  44. Spencer R, Chang G, Rees D. 'Feeling the pressure': structural insights into a gated mechanosensitive channel. Curr Opin Struct Biol. 1999;9:448-54 pubmed
    The structure determination of the large-conductance mechanosensitive channel (MscL) from Mycobacterium tuberculosis has revealed the architecture of the first full-length, gated pentameric ion channel...
  45. Tsai I, Liu Z, Rayment J, Norman C, McKinley A, Martinac B. The role of the periplasmic loop residue glutamine 65 for MscL mechanosensitivity. Eur Biophys J. 2005;34:403-12 pubmed
    The periplasmic loop of MscL, the mechanosensitive channel of large conductance, acts as a spring resisting the opening of the channel...
  46. van den Bogaart G, Krasnikov V, Poolman B. Dual-color fluorescence-burst analysis to probe protein efflux through the mechanosensitive channel MscL. Biophys J. 2007;92:1233-40 pubmed
    The mechanosensitive channel protein of large conductance, MscL, from Escherichia coli has been implicated in protein efflux, but the passage of proteins through the channel has never been demonstrated...
  47. Spencer R, Rees D. The alpha-helix and the organization and gating of channels. Annu Rev Biophys Biomol Struct. 2002;31:207-33 pubmed
    ..and other alpha-helical membrane proteins have been determined recently, including the KcsA potassium channel, the MscL mechanosensitive channel, and the AQP1 and GlpF members of the aquaporin family...
  48. Perozo E, Cortes D, Sompornpisut P, Kloda A, Martinac B. Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Nature. 2002;418:942-8 pubmed
    ..the structural rearrangements that underlie these events in the large prokaryotic mechanosensitive channel (MscL) using electron paramagnetic resonance spectroscopy and site-directed spin labelling...
  49. Ajouz B, Berrier C, Besnard M, Martinac B, Ghazi A. Contributions of the different extramembranous domains of the mechanosensitive ion channel MscL to its response to membrane tension. J Biol Chem. 2000;275:1015-22 pubmed
    b>MscL is a mechanosensitive channel that is gated by tension in the membrane bilayer alone...
  50. Okada K, Moe P, Blount P. Functional design of bacterial mechanosensitive channels. Comparisons and contrasts illuminated by random mutagenesis. J Biol Chem. 2002;277:27682-8 pubmed
    MscS and MscL are mechanosensitive channels found in bacterial plasma membranes that open large pores in response to membrane tension. These channels function to alleviate excess cell turgor invoked by rapid osmotic downshock...
  51. Perozo E, Kloda A, Cortes D, Martinac B. Site-directed spin-labeling analysis of reconstituted Mscl in the closed state. J Gen Physiol. 2001;118:193-206 pubmed
    The mechanosensitive channel from Escherichia coli (Eco-MscL) responds to membrane lateral tension by opening a large, water-filled pore that serves as an osmotic safety valve...
  52. Maurer J, Dougherty D. Generation and evaluation of a large mutational library from the Escherichia coli mechanosensitive channel of large conductance, MscL: implications for channel gating and evolutionary design. J Biol Chem. 2003;278:21076-82 pubmed
    Random mutagenesis of the mechanosensitive channel of large conductance (MscL) from Escherichia coli coupled with a high-throughput functional screen has provided new insights into channel structure and function...
  53. Chang G, Spencer R, Lee A, Barclay M, Rees D. Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science. 1998;282:2220-6 pubmed
    ..The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate ..
  54. Batiza A, Rayment I, Kung C. Channel gate! Tension, leak and disclosure. Structure. 1999;7:R99-103 pubmed
    The crystal structure of a bacterial MscL shows how this homopentameric channel protein is held tightly shut to prevent leakage whilst at rest...
  55. Maurer J, Elmore D, Lester H, Dougherty D. Comparing and contrasting Escherichia coli and Mycobacterium tuberculosis mechanosensitive channels (MscL). New gain of function mutations in the loop region. J Biol Chem. 2000;275:22238-44 pubmed
    Sequence analysis of 35 putative MscL homologues was used to develop an optimal alignment for Escherichia coli and Mycobacterium tuberculosis MscL and to place these homologues into sequence subfamilies...
  56. Batiza A, Kuo M, Yoshimura K, Kung C. Gating the bacterial mechanosensitive channel MscL invivo. Proc Natl Acad Sci U S A. 2002;99:5643-8 pubmed
    YggB and MscL are the major mechanosensitive channels in Escherichia coli, and each can rescue the double knockout mutant from osmotic downshock...
  57. Bartlett J, Li Y, Blount P. Mechanosensitive channel gating transitions resolved by functional changes upon pore modification. Biophys J. 2006;91:3684-91 pubmed
  58. Valadié H, Lacapcre J, Sanejouand Y, Etchebest C. Dynamical properties of the MscL of Escherichia coli: a normal mode analysis. J Mol Biol. 2003;332:657-74 pubmed
    The mechanosensitive channel (MscL) is an integral membrane protein which gates in response to membrane tension...
  59. Wiriyathanawudhiwong N, Ohtsu I, Li Z, Mori H, Takagi H. The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli. Appl Microbiol Biotechnol. 2009;81:903-13 pubmed publisher
    ..We concluded that TolC plays an important role in L: -cysteine tolerance probably due to its export ability and that TolC overexpression is effective for L-cysteine production in E. coli. ..
  60. Berrier C, Park K, Abes S, Bibonne A, Betton J, Ghazi A. Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent. Biochemistry. 2004;43:12585-91 pubmed
    ..We used the bacterial mechanosensitive channel MscL, a homopentamer, as a model protein...
  61. Kong Y, Shen Y, Warth T, Ma J. Conformational pathways in the gating of Escherichia coli mechanosensitive channel. Proc Natl Acad Sci U S A. 2002;99:5999-6004 pubmed
    ..Moreover, the intermediate open states of mechanosensitive channels are not symmetric, i.e., the opening does not follow iris-like motion, which sharply contrasts to the potassium channel KcsA. ..
  62. Ursell T, Huang K, Peterson E, Phillips R. Cooperative gating and spatial organization of membrane proteins through elastic interactions. PLoS Comput Biol. 2007;3:e81 pubmed
    ..We use the mechanosensitive channel of large conductance (MscL) as a case study to examine the implications of bilayer-mediated elastic interactions on protein conformational ..
  63. Romantsov T, Guan Z, Wood J. Cardiolipin and the osmotic stress responses of bacteria. Biochim Biophys Acta. 2009;1788:2092-100 pubmed publisher
    ..In contrast, a GFP-tagged variant of mechanosensitive channel MscL is not concentrated at the cell poles but anionic lipids bind to a specific site on each subunit of MscL and ..
  64. Sachs F. Retaining your identity under stress. Nat Struct Biol. 2002;9:636-7 pubmed
  65. Bezanilla F, Perozo E. Structural biology. Force and voltage sensors in one structure. Science. 2002;298:1562-3 pubmed
  66. Iscla I, Wray R, Eaton C, Blount P. Scanning MscL Channels with Targeted Post-Translational Modifications for Functional Alterations. PLoS ONE. 2015;10:e0137994 pubmed publisher
    ..The mechanosensitive channel of large conductance (MscL) from Escherichia coli was the first protein shown to encode mechanosensitive channel activity and serves as a ..