Gene Symbol: mog
Description: molybdochelatase incorporating molybdenum into molybdopterin
Alias: ECK0009, JW0008, bisD, chlG, mogA, yaaG
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Reiss J, Kleinhofs A, Klingm├╝ller W. Cloning of seven differently complementing DNA fragments with chl functions from Escherichia coli K12. Mol Gen Genet. 1987;206:352-5 pubmed
    ..The established complementation groups plus mutants of the chlD type represent eight distinct functions, which are all believed to be required for the molybdenum cofactor activity in the reduction of nitrate to nitrite by E. coli. ..
  2. del Campillo Campbell A, Campbell A. Molybdenum cofactor requirement for biotin sulfoxide reduction in Escherichia coli. J Bacteriol. 1982;149:469-78 pubmed
    ..The products of four other E. coli genes (chlA, chlB, chlE and chlG) are also needed for enzymatic activity...
  3. Neumann M, Leimkuhler S. Heavy metal ions inhibit molybdoenzyme activity by binding to the dithiolene moiety of molybdopterin in Escherichia coli. FEBS J. 2008;275:5678-89 pubmed publisher on the 6-alkyl side-chain of molybdopterin is a highly specific process that is catalysed by the MoeA and MogA proteins in Escherichia coli...
  4. Xiang S, Nichols J, Rajagopalan K, Schindelin H. The crystal structure of Escherichia coli MoeA and its relationship to the multifunctional protein gephyrin. Structure. 2001;9:299-310 pubmed
    ..The Escherichia coli MoeA and MogA proteins are involved in the final step of Moco biosynthesis: the incorporation of molybdenum into molybdopterin (..
  5. Leimkuhler S, Wuebbens M, Rajagopalan K. Characterization of Escherichia coli MoeB and its involvement in the activation of molybdopterin synthase for the biosynthesis of the molybdenum cofactor. J Biol Chem. 2001;276:34695-701 pubmed
    ..All of these exhibited activity comparable to the wild type, with the exception of mutations in cysteine residues located in putative Zn-binding motifs. For these cysteines, loss of activity correlated with loss of metal binding. ..
  6. Liu M, Wuebbens M, Rajagopalan K, Schindelin H. Crystal structure of the gephyrin-related molybdenum cofactor biosynthesis protein MogA from Escherichia coli. J Biol Chem. 2000;275:1814-22 pubmed
    ..The MogA protein exhibits affinity for molybdopterin, the organic component of Moco, and has been proposed to act as a ..
  7. Joshi M, Johnson J, Rajagopalan K. Molybdenum cofactor biosynthesis in Escherichia coli mod and mog mutants. J Bacteriol. 1996;178:4310-2 pubmed
    The molybdopterin content of Escherichia coli mod and mog mutants was estimated by conversion to the form A derivative...
  8. Stewart V, MacGregor C. Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci. J Bacteriol. 1982;151:788-99 pubmed
    ..coli which are defective with respect to nitrate reductase activity. chlE::Mu cts and chlG::Mu cts mutants were all chlorate resistant, and the strains that we examined all synthesized nitrate reductase ..
  9. Fimmel A, Haddock B. Use of chlC-lac fusions to determine regulation of gene chlC in Escherichia coli K-12. J Bacteriol. 1979;138:726-30 pubmed
    ..Gene fusions between the lac structural genes and the chlC locus were isolated, and the regulation of lac gene expression was studied. The fused lac genes were induced by nitrate anaerobically and repressed by the presence of oxygen. ..

More Information


  1. Glaser J, DeMoss J. Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol Gen Genet. 1972;116:1-10 pubmed
  2. Magalon A, Frixon C, Pommier J, Giordano G, Blasco F. In vivo interactions between gene products involved in the final stages of molybdenum cofactor biosynthesis in Escherichia coli. J Biol Chem. 2002;277:48199-204 pubmed
    ..Using a bacterial two-hybrid approach, here we report on the in vivo interactions between MogA, MoeA, MobA, and MobB implicated in several distinct although linked steps in Escherichia coli...
  3. Kozmin S, Schaaper R. Molybdenum cofactor-dependent resistance to N-hydroxylated base analogs in Escherichia coli is independent of MobA function. Mutat Res. 2007;619:9-15 pubmed
    ..Further, we establish that protection against the analogs does not require the common molybdopterin guanine-dinucleotide (MGD) form of the cofactor, but instead the guanosine monophosphate (GMP)-free version of MoCo (MPT) is sufficient. ..
  4. James R, Dean D, Debbage J. Five open reading frames upstream of the dnaK gene of E. coli. DNA Seq. 1993;3:327-32 pubmed
    ..ORF4 and htgA overlap each other on opposite strands of DNA, this would offer some interesting regulatory possibilities. ..
  5. Schrag J, Huang W, Sivaraman J, Smith C, Plamondon J, Larocque R, et al. The crystal structure of Escherichia coli MoeA, a protein from the molybdopterin synthesis pathway. J Mol Biol. 2001;310:419-31 pubmed
    ..The protein that resembles domain 3 most closely is MogA, another protein required for molybdopterin cofactor synthesis...
  6. Shanmugam K, Stewart V, Gunsalus R, Boxer D, Cole J, Chippaux M, et al. Proposed nomenclature for the genes involved in molybdenum metabolism in Escherichia coli and Salmonella typhimurium. Mol Microbiol. 1992;6:3452-4 pubmed
  7. Kamdar K, Shelton M, Finnerty V. The Drosophila molybdenum cofactor gene cinnamon is homologous to three Escherichia coli cofactor proteins and to the rat protein gephyrin. Genetics. 1994;137:791-801 pubmed
    ..Our results suggest that some portions of the prokaryotic cofactor biosynthetic pathway composed of monofunctional proteins have evolved into a multifunctional protein in higher eukaryotes. ..
  8. Nichols J, Rajagopalan K. Escherichia coli MoeA and MogA. Function in metal incorporation step of molybdenum cofactor biosynthesis. J Biol Chem. 2002;277:24995-5000 pubmed
    Escherichia coli MoeA and MogA are required for molybdenum cofactor biosynthesis and are believed to function in the addition of molybdenum to the dithiolene of molybdopterin to form molybdenum cofactor...
  9. Nichols J, Rajagopalan K. In vitro molybdenum ligation to molybdopterin using purified components. J Biol Chem. 2005;280:7817-22 pubmed
    We have previously shown that Escherichia coli MoeA and MogA are required in vivo for the final step of molybdenum cofactor biosynthesis, the addition of the molybdenum atom to the dithiolene of molybdopterin...
  10. Bevers L, Hagedoorn P, Santamaria Araujo J, Magalon A, Hagen W, Schwarz G. Function of MoaB proteins in the biosynthesis of the molybdenum and tungsten cofactors. Biochemistry. 2008;47:949-56 pubmed publisher
    ..Here, we describe the function of the Pyrococcus furiosus MoaB protein that is homologous to bacterial (such as MogA) and eukaryotic proteins (such as Cnx1) involved in the final steps of Mo cofactor synthesis...
  11. Bachmann B. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983;47:180-230 pubmed
  12. Adhya S, Cleary P, Campbell A. A deletion analysis of prophage lambda and adjacent genetic regions. Proc Natl Acad Sci U S A. 1968;61:956-62 pubmed
  13. Vergnes A, Gouffi Belhabich K, Blasco F, Giordano G, Magalon A. Involvement of the molybdenum cofactor biosynthetic machinery in the maturation of the Escherichia coli nitrate reductase A. J Biol Chem. 2004;279:41398-403 pubmed
    ..We report the first description of protein interactions between molybdenum cofactor biosynthetic proteins (MogA, MoeA, MobA, and MobB) and the aponitrate reductase (NarG) using a bacterial two-hybrid approach...