Gene Symbol: modC
Description: molybdate ABC transporter ATPase; chlorate resistance protein
Alias: ECK0754, JW0748, chlD, narD
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Johann S, Hinton S. Cloning and nucleotide sequence of the chlD locus. J Bacteriol. 1987;169:1911-6 pubmed
    The nucleotide sequence of a Sau3A1 restriction nuclease fragment that complemented an Escherichia coli chlD::Mu cts mutant strain was determined...
  2. Rech S, Deppenmeier U, Gunsalus R. Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availability. J Bacteriol. 1995;177:1023-9 pubmed
    The mod (chlD) locus at 17 min on the Escherichia coli chromosome encodes a high-affinity molybdate uptake system...
  3. Grunden A, Self W, Villain M, Blalock J, Shanmugam K. An analysis of the binding of repressor protein ModE to modABCD (molybdate transport) operator/promoter DNA of Escherichia coli. J Biol Chem. 1999;274:24308-15 pubmed
    ..The molybdate-independent mutant ModE proteins apparently mimic in its conformation the native ModE-molybdate complex, which binds to a DNA sequence motif of TATAT-7bp-TAYAT. ..
  4. Rech S, Wolin C, Gunsalus R. Properties of the periplasmic ModA molybdate-binding protein of Escherichia coli. J Biol Chem. 1996;271:2557-62 pubmed
    ..Therefore the modA gene product is essential for high affinity molybdate uptake by the cell. Tungstate interference of molybdate acquisition by the cell is apparently due in part to the high affinity of the ModA protein for this anion. ..
  5. Grunden A, Ray R, Rosentel J, Healy F, Shanmugam K. Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE. J Bacteriol. 1996;178:735-44 pubmed
    ..These results show that molybdate transport in E. coli is regulated by ModE, which acts as a repressor when bound to molybdate. ..
  6. Fimmel A, Haddock B. Use of chlC-lac fusions to determine regulation of gene chlC in Escherichia coli K-12. J Bacteriol. 1979;138:726-30 pubmed
    ..Gene fusions between the lac structural genes and the chlC locus were isolated, and the regulation of lac gene expression was studied. The fused lac genes were induced by nitrate anaerobically and repressed by the presence of oxygen. ..
  7. Hu Y, Rech S, Gunsalus R, Rees D. Crystal structure of the molybdate binding protein ModA. Nat Struct Biol. 1997;4:703-7 pubmed
  8. Walkenhorst H, Hemschemeier S, Eichenlaub R. Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene. Microbiol Res. 1995;150:347-61 pubmed
    The nucleotide sequence of a 6.8-kb chromosomal subfragment of plasmid pHW100 complementing an Escherichia coli modC (chlD) mutant has been determined...
  9. Shanmugam K, Stewart V, Gunsalus R, Boxer D, Cole J, Chippaux M, et al. Proposed nomenclature for the genes involved in molybdenum metabolism in Escherichia coli and Salmonella typhimurium. Mol Microbiol. 1992;6:3452-4 pubmed

More Information


  1. McNicholas P, Rech S, Gunsalus R. Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli. Mol Microbiol. 1997;23:515-24 pubmed
    ..These findings demonstrate that ModE acts both as a repressor and activator of the mod and moa operons, respectively, depending on the properties of the binding site. ..
  2. McNicholas P, Chiang R, Gunsalus R. The Escherichia coli modE gene: effect of modE mutations on molybdate dependent modA expression. FEMS Microbiol Lett. 1996;145:117-23 pubmed
    ..Finally, a mutational analysis of a proposed molybdate binding motif indicates that this motif does not function in regulating the repressor activity of ModE. ..
  3. del Campillo Campbell A, Campbell A. Molybdenum cofactor requirement for biotin sulfoxide reduction in Escherichia coli. J Bacteriol. 1982;149:469-78 pubmed
    ..Mutational inactivation of the chlD gene reduces the specific activity of biotin sulfoxide reductase about twofold...
  4. Adhya S, Cleary P, Campbell A. A deletion analysis of prophage lambda and adjacent genetic regions. Proc Natl Acad Sci U S A. 1968;61:956-62 pubmed
  5. Grunden A, Shanmugam K. Molybdate transport and regulation in bacteria. Arch Microbiol. 1997;168:345-54 pubmed
    ..These three proteins are coded by modA, modB, and modC genes, respectively...
  6. Joshi M, Johnson J, Rajagopalan K. Molybdenum cofactor biosynthesis in Escherichia coli mod and mog mutants. J Bacteriol. 1996;178:4310-2 pubmed
    ..The results are in accord with complete phenotypic repair of mod, and incomplete repair of mog, by culture in high concentrations of molybdate. A possible role for Mog as a molybdochelatase is discussed. ..
  7. Miyake H, Yabu H, Satoh T, Yamamoto I. Characterization and transcriptional regulation of the modABCD genes for molybdenum transport in Escherichia coli. Nucleic Acids Symp Ser. 1995;:91-2 pubmed
    ..The nucleotide sequence of 3048-base pair showed four open reading frames including the previously reported modC gene. The genes were named modA, modB, modC, and modD...
  8. Rosentel J, Healy F, Maupin Furlow J, Lee J, Shanmugam K. Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli. J Bacteriol. 1995;177:4857-64 pubmed
    ..This level was increased by over 175 times by a mutation in the modA, modB, or modC gene. The addition of molybdate to the growth medium of a mod mutant lowered phi (modA'-'lacZ+) expression...
  9. Bachmann B. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983;47:180-230 pubmed
  10. Glaser J, DeMoss J. Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol Gen Genet. 1972;116:1-10 pubmed
  11. Maupin Furlow J, Rosentel J, Lee J, Deppenmeier U, Gunsalus R, Shanmugam K. Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli. J Bacteriol. 1995;177:4851-6 pubmed
    ..second gene (modB) encodes a 229-amino-acid protein with a potential membrane location, while the 352-amino-acid ModC protein (modC product) contains a nucleotide-binding motif...
  12. Dudev T, Lim C. Oxyanion selectivity in sulfate and molybdate transport proteins: an ab initio/CDM study. J Am Chem Soc. 2004;126:10296-305 pubmed
  13. Imperial J, Hadi M, Amy N. Molybdate binding by ModA, the periplasmic component of the Escherichia coli mod molybdate transport system. Biochim Biophys Acta. 1998;1370:337-46 pubmed
    ..The affinity of ModA for molybdate was also determined in vivo and found to be similar to that determined in vitro...
  14. B bien M, Kirsch J, M jean V, Verm glio A. Involvement of a putative molybdenum enzyme in the reduction of selenate by Escherichia coli. Microbiology. 2002;148:3865-72 pubmed publisher
    ..In addition, all the various mutants were still able to reduce selenite into elemental selenium. This implies that the transport and reduction of this compound are clearly distinct from those of selenate...