Gene Symbol: modB
Description: molybdate ABC transporter permease; chlorate resistance protein
Alias: ECK0753, JW0747, chlJ
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Hu Y, Rech S, Gunsalus R, Rees D. Crystal structure of the molybdate binding protein ModA. Nat Struct Biol. 1997;4:703-7 pubmed
  2. Grunden A, Self W, Villain M, Blalock J, Shanmugam K. An analysis of the binding of repressor protein ModE to modABCD (molybdate transport) operator/promoter DNA of Escherichia coli. J Biol Chem. 1999;274:24308-15 pubmed
    ..The molybdate-independent mutant ModE proteins apparently mimic in its conformation the native ModE-molybdate complex, which binds to a DNA sequence motif of TATAT-7bp-TAYAT. ..
  3. Rech S, Deppenmeier U, Gunsalus R. Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availability. J Bacteriol. 1995;177:1023-9 pubmed
    ..Mutations within this sequence resulted in nearly complete derepression of gene expression and suggest that transcription of the operon is mediated by a molybdenum-responsive regulatory protein. ..
  4. Grunden A, Ray R, Rosentel J, Healy F, Shanmugam K. Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE. J Bacteriol. 1996;178:735-44 pubmed
    ..ModE, in the presence of 1 mM molybdate, repressed the production of plasmid-encoded ModA and ModB' proteins in an in vitro transcription-translation system...
  5. Walkenhorst H, Hemschemeier S, Eichenlaub R. Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene. Microbiol Res. 1995;150:347-61 pubmed
    ..The products of modB (chlJ) and modC (chlD) have been described earlier as the inner membrane protein and the ATP-binding protein of the ..
  6. Johann S, Hinton S. Cloning and nucleotide sequence of the chlD locus. J Bacteriol. 1987;169:1911-6 pubmed
    ..A proposal that the chlD locus is the molybdate transport operon is discussed in terms of the chlD phenotype. ..
  7. Rech S, Wolin C, Gunsalus R. Properties of the periplasmic ModA molybdate-binding protein of Escherichia coli. J Biol Chem. 1996;271:2557-62 pubmed
    ..Therefore the modA gene product is essential for high affinity molybdate uptake by the cell. Tungstate interference of molybdate acquisition by the cell is apparently due in part to the high affinity of the ModA protein for this anion. ..
  8. Dudev T, Lim C. Oxyanion selectivity in sulfate and molybdate transport proteins: an ab initio/CDM study. J Am Chem Soc. 2004;126:10296-305 pubmed
  9. Imperial J, Hadi M, Amy N. Molybdate binding by ModA, the periplasmic component of the Escherichia coli mod molybdate transport system. Biochim Biophys Acta. 1998;1370:337-46 pubmed
    ..The affinity of ModA for molybdate was also determined in vivo and found to be similar to that determined in vitro...

More Information


  1. Grunden A, Shanmugam K. Molybdate transport and regulation in bacteria. Arch Microbiol. 1997;168:345-54 pubmed
    ..These three proteins are coded by modA, modB, and modC genes, respectively...
  2. Rosentel J, Healy F, Maupin Furlow J, Lee J, Shanmugam K. Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli. J Bacteriol. 1995;177:4857-64 pubmed
    ..This level was increased by over 175 times by a mutation in the modA, modB, or modC gene. The addition of molybdate to the growth medium of a mod mutant lowered phi (modA'-'lacZ+) expression...
  3. Maupin Furlow J, Rosentel J, Lee J, Deppenmeier U, Gunsalus R, Shanmugam K. Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli. J Bacteriol. 1995;177:4851-6 pubmed
    ..The second gene (modB) encodes a 229-amino-acid protein with a potential membrane location, while the 352-amino-acid ModC protein (modC ..
  4. McNicholas P, Rech S, Gunsalus R. Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli. Mol Microbiol. 1997;23:515-24 pubmed
    ..These findings demonstrate that ModE acts both as a repressor and activator of the mod and moa operons, respectively, depending on the properties of the binding site. ..
  5. Miyake H, Yabu H, Satoh T, Yamamoto I. Characterization and transcriptional regulation of the modABCD genes for molybdenum transport in Escherichia coli. Nucleic Acids Symp Ser. 1995;:91-2 pubmed
    ..The genes were named modA, modB, modC, and modD...
  6. Joshi M, Johnson J, Rajagopalan K. Molybdenum cofactor biosynthesis in Escherichia coli mod and mog mutants. J Bacteriol. 1996;178:4310-2 pubmed
    ..The results are in accord with complete phenotypic repair of mod, and incomplete repair of mog, by culture in high concentrations of molybdate. A possible role for Mog as a molybdochelatase is discussed. ..
  7. McNicholas P, Chiang R, Gunsalus R. The Escherichia coli modE gene: effect of modE mutations on molybdate dependent modA expression. FEMS Microbiol Lett. 1996;145:117-23 pubmed
    ..Finally, a mutational analysis of a proposed molybdate binding motif indicates that this motif does not function in regulating the repressor activity of ModE. ..
  8. Shanmugam K, Stewart V, Gunsalus R, Boxer D, Cole J, Chippaux M, et al. Proposed nomenclature for the genes involved in molybdenum metabolism in Escherichia coli and Salmonella typhimurium. Mol Microbiol. 1992;6:3452-4 pubmed