Gene Symbol: moaA
Description: molybdopterin biosynthesis protein A
Alias: ECK0770, JW0764, bisA, chlA, narA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Fimmel A, Haddock B. Use of chlC-lac fusions to determine regulation of gene chlC in Escherichia coli K-12. J Bacteriol. 1979;138:726-30 pubmed
    ..Gene fusions between the lac structural genes and the chlC locus were isolated, and the regulation of lac gene expression was studied. The fused lac genes were induced by nitrate anaerobically and repressed by the presence of oxygen. ..
  2. Baker K, Boxer D. Regulation of the chlA locus of Escherichia coli K12: involvement of molybdenum cofactor. Mol Microbiol. 1991;5:901-7 pubmed
    The chlA locus encodes functions required for the biosynthesis of the molybdopterin part of the molybdenum cofactor...
  3. Santamaria Araujo J, Fischer B, Otte T, Nimtz M, Mendel R, Wray V, et al. The tetrahydropyranopterin structure of the sulfur-free and metal-free molybdenum cofactor precursor. J Biol Chem. 2004;279:15994-9 pubmed
    ..characterization and structure elucidation of precursor Z purified from Escherichia coli overproducing MoaA and MoaC, two proteins essential for bacterial precursor Z synthesis...
  4. Shanmugam K, Stewart V, Gunsalus R, Boxer D, Cole J, Chippaux M, et al. Proposed nomenclature for the genes involved in molybdenum metabolism in Escherichia coli and Salmonella typhimurium. Mol Microbiol. 1992;6:3452-4 pubmed
  5. Anderson L, McNairn E, Lubke T, Pau R, Boxer D, Leubke T. ModE-dependent molybdate regulation of the molybdenum cofactor operon moa in Escherichia coli. J Bacteriol. 2000;182:7035-43 pubmed
    ..Transcription of moa is controlled at two sigma-70-type promoters immediately upstream of the moaA gene...
  6. Regulski E, Moy R, Weinberg Z, Barrick J, Yao Z, Ruzzo W, et al. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism. Mol Microbiol. 2008;68:918-32 pubmed publisher
    ..Furthermore, we identify variants of this RNA that are likely to be triggered by the related tungsten cofactor (Tuco), which carries tungsten in place of molybdenum as the metal constituent...
  7. Johnson M, Rajagopalan K. Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis. J Bacteriol. 1987;169:117-25 pubmed
    ..By P1 mapping, these two mutations mapped to chlA and chlE, loci previously postulated but never definitely shown to be involved in MPT biosynthesis...
  8. Burgis N, Brucker J, Cunningham R. Repair system for noncanonical purines in Escherichia coli. J Bacteriol. 2003;185:3101-10 pubmed
  9. Kozmin S, Schaaper R. Molybdenum cofactor-dependent resistance to N-hydroxylated base analogs in Escherichia coli is independent of MobA function. Mutat Res. 2007;619:9-15 pubmed
    ..Further, we establish that protection against the analogs does not require the common molybdopterin guanine-dinucleotide (MGD) form of the cofactor, but instead the guanosine monophosphate (GMP)-free version of MoCo (MPT) is sufficient. ..

More Information


  1. Kuper J, Meyer zu Berstenhorst S, Vödisch B, Mendel R, Schwarz G, Boxer D. In vivo detection of molybdate-binding proteins using a competition assay with ModE in Escherichia coli. FEMS Microbiol Lett. 2003;218:187-93 pubmed
  2. Sofia H, Chen G, Hetzler B, Reyes Spindola J, Miller N. Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods. Nucleic Acids Res. 2001;29:1097-106 pubmed
    ..Two functional predictions for unknown proteins are made based on integrating other data types such as motif, domain, operon and biochemical pathway into an organized view of similarity relationships. ..
  3. Venables W, Guest J. Transduction of nitrate reductase loci of Escherichia coli by phages P-1 and lambda. Mol Gen Genet. 1968;103:127-40 pubmed
  4. Lambdren P, Guest J. A novel method for isolating chlorate-resistant mutants of Escherichia coli K12 by anaerobic selection on a lactate plus fumarate medium. J Gen Microbiol. 1976;93:173-6 pubmed
  5. Glaser J, DeMoss J. Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol Gen Genet. 1972;116:1-10 pubmed
  6. Reiss J, Kleinhofs A, Klingmüller W. Cloning of seven differently complementing DNA fragments with chl functions from Escherichia coli K12. Mol Gen Genet. 1987;206:352-5 pubmed
    ..Selection for growth on nitrate under anaerobic conditions yielded four plasmids which complemented mutants of the chlA, B, E and G types. The chromosomal fragments were mapped with restriction enzymes and subcloned...
  7. Bachmann B. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983;47:180-230 pubmed
  8. Layer G, Kervio E, Morlock G, Heinz D, Jahn D, Retey J, et al. Structural and functional comparison of HemN to other radical SAM enzymes. Biol Chem. 2005;386:971-80 pubmed
    ..The enzymes are compared to other radical SAM enzymes and in particular the three reported crystal structures from this family, HemN, biotin synthase and MoaA, are discussed.
  9. Rivers S, McNairn E, Blasco F, Giordano G, Boxer D. Molecular genetic analysis of the moa operon of Escherichia coli K-12 required for molybdenum cofactor biosynthesis. Mol Microbiol. 1993;8:1071-81 pubmed
    ..The encoded proteins (MoaA-MoaE) have predicted molecular weights of 37,346, 18,665, 17,234, 8843 and 16,981 respectively...
  10. Rieder C, Eisenreich W, O Brien J, Richter G, Götze E, Boyle P, et al. Rearrangement reactions in the biosynthesis of molybdopterin--an NMR study with multiply 13C/15N labelled precursors. Eur J Biochem. 1998;255:24-36 pubmed
    ..Carbon atom C1' of compound Z is derived from carbon atom C8 of a guanine derivative. The remodeling of the carbon skeleton of the pentose and purine moieties proceed via intramolecular rearrangement reactions. ..
  11. Miller J, Amy N. Molybdenum cofactor in chlorate-resistant and nitrate reductase-deficient insertion mutants of Escherichia coli. J Bacteriol. 1983;155:793-801 pubmed
    ..The chlA and chlE mutants had no detectable cofactor...
  12. Alper M, Ames B. Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions. J Bacteriol. 1975;121:259-66 pubmed
    ..Through simultaneous selection for mutations in the two closely linked genes, gal and chlA, a variety of deletions of varying length, some extending through as much as 1 min of the chromosome, could be ..
  13. McNicholas P, Rech S, Gunsalus R. Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli. Mol Microbiol. 1997;23:515-24 pubmed
    ..Footprint analysis of the moaA promoter revealed a single protected region located immediately upstream of the putative -35 consensus sequence and ..
  14. Adhya S, Cleary P, Campbell A. A deletion analysis of prophage lambda and adjacent genetic regions. Proc Natl Acad Sci U S A. 1968;61:956-62 pubmed
  15. MacGregor C. Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli. J Bacteriol. 1975;121:1117-21 pubmed
    ..b>chlA and B mutants contained all three subunits present in the wild-type enzyme...
  16. del Campillo Campbell A, Campbell A. Molybdenum cofactor requirement for biotin sulfoxide reduction in Escherichia coli. J Bacteriol. 1982;149:469-78 pubmed
    ..Mutations previously assigned to the bisA, bisB, and bisD genes belong to genes chlA, chlE, and chlG, respectively...