Gene Symbol: mltF
Description: membrane-bound lytic transglycosylase F, murein hydrolase
Alias: ECK2556, JW2542, yfhD
Species: Escherichia coli str. K-12 substr. MG1655
Schendel F, Mueller E, Stubbe J, Shiau A, Smith J. Formylglycinamide ribonucleotide synthetase from Escherichia coli: cloning, sequencing, overproduction, isolation, and characterization. Biochemistry. 1989;28:2459-71 pubmed
..Incubation of [18O]FGAR with enzyme, ATP, and glutamine results in quantitative transfer of the 18O to Pi. ..
Bennik M, Pomposiello P, Thorne D, Demple B. Defining a rob regulon in Escherichia coli by using transposon mutagenesis. J Bacteriol. 2000;182:3794-801 pubmed
..activated by normal expression of Rob from the chromosomal rob gene (inaA, marR, aslB, ybaO, mdlA, yfhD, and ybiS). One gene, galT, was repressed by Rob...
Scheurwater E, Clarke A. The C-terminal domain of Escherichia coli YfhD functions as a lytic transglycosylase. J Biol Chem. 2008;283:8363-73 pubmed publisher
..Based on these data, the enzyme was renamed membrane-bound lytic transglycosylase F. The modular structure of MltF was investigated through genetic engineering for the separate production of identified N-terminal and C-terminal ..
Poulsen L, Larsen N, Molin S, Andersson P. Analysis of an Escherichia coli mutant strain resistant to the cell-killing function encoded by the gef gene family. Mol Microbiol. 1992;6:895-905 pubmed
..1 kDa. ORF178 and an upstream frame, ORF190, probably constitute an operon...
Koonin E, Rudd K. A conserved domain in putative bacterial and bacteriophage transglycosylases. Trends Biochem Sci. 1994;19:106-7 pubmed
Madoori P, Thunnissen A. Purification, crystallization and preliminary X-ray diffraction analysis of the lytic transglycosylase MltF from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:534-8 pubmed publisher
The lytic transglycosylase MltF from Escherichia coli is an outer-membrane-bound periplasmic protein with two domains: a C-terminal catalytic domain with a lysozyme-like fold and an N-terminal domain of unknown function that is homologous ..
Lee M, Hesek D, Llarrull L, Lastochkin E, Pi H, Boggess B, et al
. Reactions of all Escherichia coli lytic transglycosylases with bacterial cell wall. J Am Chem Soc. 2013;135:3311-4 pubmed publisher
..These discoveries underscore the importance of the functions of these enzymes for recycling of the cell wall. ..