Gene Symbol: mipA
Description: scaffolding protein for murein synthesizing machinery
Alias: ECK1780, JW1771, yeaF
Species: Escherichia coli str. K-12 substr. MG1655
Bertsche U, Breukink E, Kast T, Vollmer W. In vitro murein peptidoglycan synthesis by dimers of the bifunctional transglycosylase-transpeptidase PBP1B from Escherichia coli. J Biol Chem. 2005;280:38096-101 pubmed
..PBP1B was also capable of synthesizing trimeric muropeptide structures. Tri-, tetra-, and pentapeptide compounds could serve as acceptors in the PBP1B-catalyzed transpeptidation reaction. ..
Thompson K, Rhodius V, Gottesman S. SigmaE regulates and is regulated by a small RNA in Escherichia coli. J Bacteriol. 2007;189:4243-56 pubmed
..One contained the gene for the response regulator OmpR; the second contained mipA, encoding a murein hydrolase...
Vollmer W, von Rechenberg M, Holtje J. Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli. J Biol Chem. 1999;274:6726-34 pubmed
..coli genome. This MltA-interacting protein, named MipA, in addition binds to PBP1B, a bifunctional murein transglycosylase/transpeptidase...
Molloy M, Herbert B, Slade M, Rabilloud T, Nouwens A, Williams K, et al
. Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem. 2000;267:2871-81 pubmed
..Two of the identified OMPs (YbiL, YeaF) were previously known only from their ORFs, and their identification confirms the cognate genes are transcribed ..
Han M, Lee S. The Escherichia coli proteome: past, present, and future prospects. Microbiol Mol Biol Rev. 2006;70:362-439 pubmed
..Here, we review the past development and current status of E. coli proteome research in terms of its biological, biotechnological, and methodological significance and suggest future prospects. ..
Elad T, Benovich E, Magrisso S, Belkin S. Toxicant identification by a luminescent bacterial bioreporter panel: application of pattern classification algorithms. Environ Sci Technol. 2008;42:8486-91 pubmed
..Escherichia coli reporter strains harboring fusions of selected gene promoters (grpE, nhoA, oraA, lacZ, and mipA) to luxCDABE were exposed to five model toxicants and to a toxicant-free control in a 40-repetition format...
Han M, Lee S, Hong S. Comparative analysis of envelope proteomes in Escherichia coli B and K-12 strains. J Microbiol Biotechnol. 2012;22:470-8 pubmed
..Thus, these findings suggest that E. coli K-12 and its derivatives will be more favorable strains in certain biotechnological applications, such as cell surface display or membrane engineering studies. ..
Li H, Zhang D, Lin X, Peng X. Outer membrane proteomics of kanamycin-resistant Escherichia coli identified MipA as a novel antibiotic resistance-related protein. FEMS Microbiol Lett. 2015;362: pubmed publisher
..spectrometry and western blotting results revealed that OM proteins TolC, Tsx and OstA were up-regulated, whereas MipA, OmpA, FadL and OmpW were down-regulated in kanamycin-resistant E. coli K-12 strain...
Arnold C, McElhanon J, Lee A, Leonhart R, Siegele D. Global analysis of Escherichia coli gene expression during the acetate-induced acid tolerance response. J Bacteriol. 2001;183:2178-86 pubmed
..Treatment with either NaCl or sodium acetate (pH 7.0) increased expression of an rpoS::lacZ fusion protein, but only treatment with acetate increased acid survival. ..