minE

Summary

Gene Symbol: minE
Description: cell division topological specificity factor
Alias: ECK1162, JW1163, minB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Szeto J, Eng N, Acharya S, Rigden M, Dillon J. A conserved polar region in the cell division site determinant MinD is required for responding to MinE-induced oscillation but not for localization within coiled arrays. Res Microbiol. 2005;156:17-29 pubmed
    A region in the cell division site determinant MinD required for stimulation by MinE and which determines MinD topological specificity along coil-like structures has been identified...
  2. Derr J, Hopper J, Sain A, Rutenberg A. Self-organization of the MinE protein ring in subcellular Min oscillations. Phys Rev E Stat Nonlin Soft Matter Phys. 2009;80:011922 pubmed
    We model the self-organization of the MinE ring that is observed during subcellular oscillations of the proteins MinD and MinE within the rod-shaped bacterium Escherichia coli...
  3. Ma L, King G, Rothfield L. Mapping the MinE site involved in interaction with the MinD division site selection protein of Escherichia coli. J Bacteriol. 2003;185:4948-55 pubmed
    Interactions between the MinD and MinE proteins are required for proper placement of the Escherichia coli division septum...
  4. Suefuji K, Valluzzi R, RayChaudhuri D. Dynamic assembly of MinD into filament bundles modulated by ATP, phospholipids, and MinE. Proc Natl Acad Sci U S A. 2002;99:16776-81 pubmed publisher
    ..of MinD ATPase between the polar halves of the cell membrane, together with the division inhibitor MinC, under MinE control. The mechanism underlying MinD oscillation remains poorly understood...
  5. de Boer P, Crossley R, Rothfield L. A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli. Cell. 1989;56:641-9 pubmed
    The E. coli minicell locus (minB) was shown to code for three gene products (MinC, MinD, and MinE) whose coordinate action is required for proper placement of the division spetum...
  6. Hu Z, Gogol E, Lutkenhaus J. Dynamic assembly of MinD on phospholipid vesicles regulated by ATP and MinE. Proc Natl Acad Sci U S A. 2002;99:6761-6 pubmed publisher
    ..the min system and requires the rapid oscillation of MinD between the two halves of the cell under the control of MinE. In this study we have further investigated the molecular basis for this oscillation by examining the interaction ..
  7. Kruse K. A dynamic model for determining the middle of Escherichia coli. Biophys J. 2002;82:618-27 pubmed publisher
    ..In Escherichia coli, this process depends crucially on the proteins MinC, MinD, and MinE. The detailed mechanism by which these proteins determine the correct position of the division plane is currently ..
  8. Raskin D, de Boer P. Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli. Proc Natl Acad Sci U S A. 1999;96:4971-6 pubmed
    ..the combined action of a general division inhibitor (MinC), a site-specific suppressor of division inhibition (MinE), and an ATPase (MinD) that is required for proper functioning of both MinC and MinE...
  9. Zhao C, de Boer P, Rothfield L. Proper placement of the Escherichia coli division site requires two functions that are associated with different domains of the MinE protein. Proc Natl Acad Sci U S A. 1995;92:4313-7 pubmed
    The proper placement of the Escherichia coli division septum requires the MinE protein. MinE accomplishes this by imparting topological specificity to a division inhibitor coded by the minC and minD genes...

More Information

Publications78

  1. Shih Y, Fu X, King G, Le T, Rothfield L. Division site placement in E.coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains. EMBO J. 2002;21:3347-57 pubmed publisher
    The MinE protein functions as a topological specificity factor in determining the site of septal placement in Escherichia coli...
  2. Meinhardt H, de Boer P. Pattern formation in Escherichia coli: a model for the pole-to-pole oscillations of Min proteins and the localization of the division site. Proc Natl Acad Sci U S A. 2001;98:14202-7 pubmed publisher
    ..i) the MinD ATPase self-assembles on the membrane and recruits both MinC, an inhibitor of Z ring formation, and MinE, a protein required for MinC/MinD oscillation, (ii) a local accumulation of MinE is generated by a pattern ..
  3. Corbin B, Yu X, Margolin W. Exploring intracellular space: function of the Min system in round-shaped Escherichia coli. EMBO J. 2002;21:1998-2008 pubmed publisher
    ..A MinE-GFP fusion exhibited similar behavior...
  4. Fu X, Shih Y, Zhang Y, Rothfield L. The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle. Proc Natl Acad Sci U S A. 2001;98:980-5 pubmed
    Placement of the division site at midcell in Escherichia coli requires the MinE protein...
  5. Hale C, Meinhardt H, de Boer P. Dynamic localization cycle of the cell division regulator MinE in Escherichia coli. EMBO J. 2001;20:1563-72 pubmed
    ..MinD and MinE regulate MinC activity by modulating its cellular location in a unique fashion...
  6. King G, Rowland S, Pan B, Mackay J, Mullen G, Rothfield L. The dimerization and topological specificity functions of MinE reside in a structurally autonomous C-terminal domain. Mol Microbiol. 1999;31:1161-9 pubmed
    ..of the division septum in Escherichia coli requires the co-ordinated action of three proteins, MinC, MinD and MinE. MinC and MinD interact to form a non-specific division inhibitor that blocks septation at all potential division ..
  7. Shih Y, Kawagishi I, Rothfield L. The MreB and Min cytoskeletal-like systems play independent roles in prokaryotic polar differentiation. Mol Microbiol. 2005;58:917-28 pubmed
    ..Thus, the two bacterial cytoskeletal-like systems act independently on different aspects of cell polarization. ..
  8. Ramirez Arcos S, Szeto J, Dillon J, Margolin W. Conservation of dynamic localization among MinD and MinE orthologues: oscillation of Neisseria gonorrhoeae proteins in Escherichia coli. Mol Microbiol. 2002;46:493-504 pubmed
    ..coli. Green fluorescent protein (GFP) fusions to gonococcal MinD and MinE localized dynamically in different E. coli backgrounds. GFP-MinDNg moved from pole to pole in rod-shaped E...
  9. Howard M, Rutenberg A, de Vet S. Dynamic compartmentalization of bacteria: accurate division in E. coli. Phys Rev Lett. 2001;87:278102 pubmed publisher
    ..coli is controlled by the min system of proteins: MinC, MinD, and MinE. These proteins coherently oscillate from end to end of the bacterium...
  10. King G, Shih Y, Maciejewski M, Bains N, Pan B, Rowland S, et al. Structural basis for the topological specificity function of MinE. Nat Struct Biol. 2000;7:1013-7 pubmed publisher
    ..positioning of the division septum in Escherichia coli depends on the coordinated action of the MinC, MinD and MinE proteins...
  11. Kerr R, Levine H, Sejnowski T, Rappel W. Division accuracy in a stochastic model of Min oscillations in Escherichia coli. Proc Natl Acad Sci U S A. 2006;103:347-52 pubmed
    Accurate cell division in Escherichia coli requires the Min proteins MinC, MinD, and MinE as well as the presence of nucleoids...
  12. Drew D, Osborn M, Rothfield L. A polymerization-depolymerization model that accurately generates the self-sustained oscillatory system involved in bacterial division site placement. Proc Natl Acad Sci U S A. 2005;102:6114-8 pubmed
    ..involves a unique spatial oscillatory system in which membrane-associated structures composed of the MinC, MinD and MinE proteins oscillate rapidly between the two cell poles...
  13. Tostevin F, Howard M. A stochastic model of Min oscillations in Escherichia coli and Min protein segregation during cell division. Phys Biol. 2005;3:1-12 pubmed
    ..The fractions of Min proteins in the daughter cells vary widely, from 50%-50% up to 85%-15% of the total from the parent cell, suggesting that there may be another mechanism for regulating these levels in vivo. ..
  14. Kruse K, Howard M, Margolin W. An experimentalist's guide to computational modelling of the Min system. Mol Microbiol. 2007;63:1279-84 pubmed
    ..We also review the effects of fluctuations caused by low cellular concentration of Min proteins, and describe how stochastic effects may potentially influence Min protein dynamics. ..
  15. Lackner L, Raskin D, de Boer P. ATP-dependent interactions between Escherichia coli Min proteins and the phospholipid membrane in vitro. J Bacteriol. 2003;185:735-49 pubmed
    ..involves a membrane association-dissociation cycle that is driven by the activities of the MinD ATPase and the MinE topological specificity factor, which themselves undergo coupled oscillatory localization cycles...
  16. Howard M, Rutenberg A. Pattern formation inside bacteria: fluctuations due to the low copy number of proteins. Phys Rev Lett. 2003;90:128102 pubmed publisher
    ..We find that, for some parameter regions, the protein concentrations are low enough that fluctuations are essential for the generation of patterns. We also examine the role of fluctuations in constraining protein concentration levels...
  17. Huang K, Meir Y, Wingreen N. Dynamic structures in Escherichia coli: spontaneous formation of MinE rings and MinD polar zones. Proc Natl Acad Sci U S A. 2003;100:12724-8 pubmed
    ..The dynamic oscillatory structures produced by the Min proteins, including a ring of MinE protein, compact polar zones of MinD, and zebra-striped oscillations in filamentous cells, remain unexplained...
  18. Raskin D, de Boer P. The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli. Cell. 1997;91:685-94 pubmed
    ..MinCD) and of a topological specificity factor that somehow prevents MinCD action at the middle of the cell (MinE)...
  19. Raskin D, de Boer P. MinDE-dependent pole-to-pole oscillation of division inhibitor MinC in Escherichia coli. J Bacteriol. 1999;181:6419-24 pubmed
    ..MinC activity requires that of MinD, and the MinE peptide provides topological specificity by suppressing MinC-MinD-mediated division inhibition specifically at the ..
  20. Lutkenhaus J. Assembly dynamics of the bacterial MinCDE system and spatial regulation of the Z ring. Annu Rev Biochem. 2007;76:539-62 pubmed
    ..Additional gradients of negative regulators of FtsZ assembly are used by E. coli and other bacteria to achieve spatial control of Z-ring assembly. ..
  21. de Boer P, Crossley R, Rothfield L. Isolation and properties of minB, a complex genetic locus involved in correct placement of the division site in Escherichia coli. J Bacteriol. 1988;170:2106-12 pubmed
    Mutation of Escherichia coli minicell locus (minB) results in aberrant placement of the division septum. In this paper we report the isolation and characterization of the minB locus...
  22. Hsieh C, Lin T, Lai H, Lin C, Hsieh T, Shih Y. Direct MinE-membrane interaction contributes to the proper localization of MinDE in E. coli. Mol Microbiol. 2010;75:499-512 pubmed publisher
    ..In addition to stimulating MinD ATPase activity, we report here that MinE can directly interact with the membrane and this interaction contributes to the proper MinDE localization and ..
  23. Lutkenhaus J. Min oscillation in bacteria. Adv Exp Med Biol. 2008;641:49-61 pubmed
    ..The simplicity and ease of manipulating the Min system make it a tractable model to obtain a complete understanding of a self-organizing system. ..
  24. Shih Y, Le T, Rothfield L. Division site selection in Escherichia coli involves dynamic redistribution of Min proteins within coiled structures that extend between the two cell poles. Proc Natl Acad Sci U S A. 2003;100:7865-70 pubmed publisher
    ..coli and that structures of this type can undergo dynamic changes that play important roles in division site placement and possibly other aspects of the life of the cell...
  25. Hu Z, Lutkenhaus J. Topological regulation of cell division in E. coli. spatiotemporal oscillation of MinD requires stimulation of its ATPase by MinE and phospholipid. Mol Cell. 2001;7:1337-43 pubmed
    ..This positioning is achieved through a rapid oscillation of MinC from pole to pole, a process requiring MinD and MinE. However, the mechanistic basis for this oscillation is not known...
  26. Hu Z, Lutkenhaus J. Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE. Mol Microbiol. 1999;34:82-90 pubmed
    ..through the action of an inhibitor of division, MinC, that is activated by MinD and topologically regulated by MinE. In this study, we have used a functional GFP-MinC fusion to monitor the location of MinC...
  27. Rowland S, Fu X, Sayed M, Zhang Y, Cook W, Rothfield L. Membrane redistribution of the Escherichia coli MinD protein induced by MinE. J Bacteriol. 2000;182:613-9 pubmed
    ..Selection of the correct division site at midcell is controlled by three proteins: MinC, MinD, and MinE. It has previously been shown (D. Raskin and P...
  28. Justice S, Garcia Lara J, Rothfield L. Cell division inhibitors SulA and MinC/MinD block septum formation at different steps in the assembly of the Escherichia coli division machinery. Mol Microbiol. 2000;37:410-23 pubmed
    ..These results indicate that MinCD blocks the assembly of the septation machinery at a later step than SulA, at the stage at which FtsA is added to the FtsZ ring. ..
  29. Loose M, Fischer Friedrich E, Ries J, Kruse K, Schwille P. Spatial regulators for bacterial cell division self-organize into surface waves in vitro. Science. 2008;320:789-92 pubmed publisher
    ..We present a reaction-diffusion model of the MinD and MinE dynamics that accounts for our experimental observations and also captures the in vivo oscillations.
  30. Pichoff S, Vollrath B, Touriol C, Bouche J. Deletion analysis of gene minE which encodes the topological specificity factor of cell division in Escherichia coli. Mol Microbiol. 1995;18:321-9 pubmed
    Division inhibition caused by the minCD gene products of Escherichia coli is suppressed specifically at mid-cell by MinE protein expressed at physiological levels...
  31. Zhou H, Schulze R, Cox S, Saez C, Hu Z, Lutkenhaus J. Analysis of MinD mutations reveals residues required for MinE stimulation of the MinD ATPase and residues required for MinC interaction. J Bacteriol. 2005;187:629-38 pubmed
    ..b>MinE, which is also recruited to the membrane by MinD, displaces MinC and stimulates the MinD ATPase, resulting in the ..
  32. Ma L, King G, Rothfield L. Positioning of the MinE binding site on the MinD surface suggests a plausible mechanism for activation of the Escherichia coli MinD ATPase during division site selection. Mol Microbiol. 2004;54:99-108 pubmed
    Division site selection in Escherichia coli requires that the MinD protein interact with itself and with MinC and MinE. MinD is a member of the NifH-ArsA-Par-MinD subgroup of ATPases...
  33. Shih Y, Huang K, Lai H, Liao J, Lee C, Chang C, et al. The N-terminal amphipathic helix of the topological specificity factor MinE is associated with shaping membrane curvature. PLoS ONE. 2011;6:e21425 pubmed publisher
    ..Direct interaction of MinE with the cell membrane is critical for the dynamic behavior of the Min system...
  34. Meacci G, Ries J, Fischer Friedrich E, Kahya N, Schwille P, Kruse K. Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy. Phys Biol. 2006;3:255-63 pubmed
    ..Escherichia coli, selection of the division site involves pole-to-pole oscillations of the proteins MinD and MinE. Different oscillation mechanisms based on cooperative effects between Min-proteins and on the exchange of Min-..
  35. Pichoff S, Alibaud L, Guédant A, Castanié M, Bouche J. An Escherichia coli gene (yaeO) suppresses temperature-sensitive mutations in essential genes by modulating Rho-dependent transcription termination. Mol Microbiol. 1998;29:859-69 pubmed
    An extragenic multicopy suppressor of the cell division inhibition caused by a MalE-MinE fusion protein in Escherichia coli has been mapped and identified as yaeO, one of the two short open reading frames (ORFs) of an operon located at 4...
  36. Borowski P, Cytrynbaum E. Predictions from a stochastic polymer model for the MinDE protein dynamics in Escherichia coli. Phys Rev E Stat Nonlin Soft Matter Phys. 2009;80:041916 pubmed
    ..The model provides an interesting example of a stochastic hybrid system that is, in some limits, analytically tractable. ..
  37. Bi E, Lutkenhaus J. Interaction between the min locus and ftsZ. J Bacteriol. 1990;172:5610-6 pubmed
    In Escherichia coli, distinct but similar minicell phenotypes resulting from mutation at the minB locus and increased expression of ftsZ suggested a possible interaction between these genes...
  38. Hu Z, Saez C, Lutkenhaus J. Recruitment of MinC, an inhibitor of Z-ring formation, to the membrane in Escherichia coli: role of MinD and MinE. J Bacteriol. 2003;185:196-203 pubmed
    ..Addition of MinE to the MinCD-bilayer complex resulted in release of both MinC and MinD...
  39. Park K, Wu W, Battaile K, Lovell S, Holyoak T, Lutkenhaus J. The Min oscillator uses MinD-dependent conformational changes in MinE to spatially regulate cytokinesis. Cell. 2011;146:396-407 pubmed publisher
    In E. coli, MinD recruits MinE to the membrane, leading to a coupled oscillation required for spatial regulation of the cytokinetic Z ring...
  40. Roos M, van Geel A, Aarsman M, Veuskens J, Woldringh C, Nanninga N. The replicated ftsQAZ and minB chromosomal regions of Escherichia coli segregate on average in line with nucleoid movement. Mol Microbiol. 2001;39:633-40 pubmed
    The average cellular positions of the ftsQAZ region (2 min) and the minB region (26.5 min) during the cell cycle was determined by fluorescent in situ hybridization using the position of oriC as a reference point...
  41. Jaffe A, D Ari R, Hiraga S. Minicell-forming mutants of Escherichia coli: production of minicells and anucleate rods. J Bacteriol. 1988;170:3094-101 pubmed
    The Escherichia coli minB mutant originally isolated is known to septate at cell poles to form spherical anucleate minicells...
  42. Touhami A, Jericho M, Rutenberg A. Temperature dependence of MinD oscillation in Escherichia coli: running hot and fast. J Bacteriol. 2006;188:7661-7 pubmed
    ..A quantitative model of MinDE oscillation exhibited similar behavior, with an activated temperature dependence of the MinE-stimulated MinD-ATPase rate.
  43. Walsh J, Angstmann C, Duggin I, Curmi P. Non-linear Min protein interactions generate harmonics that signal mid-cell division in Escherichia coli. PLoS ONE. 2017;12:e0185947 pubmed publisher
    The Min protein system creates a dynamic spatial pattern in Escherichia coli cells where the proteins MinD and MinE oscillate from pole to pole...
  44. Bramkamp M, van Baarle S. Division site selection in rod-shaped bacteria. Curr Opin Microbiol. 2009;12:683-8 pubmed publisher
    ..However, recent advances show that at least the Min system also ensures that division occurs only once in a cell cycle and might also act downstream of FtsZ assembly. ..
  45. Renner L, Weibel D. MinD and MinE interact with anionic phospholipids and regulate division plane formation in Escherichia coli. J Biol Chem. 2012;287:38835-44 pubmed publisher
    The Min proteins (MinC, MinD, and MinE) form a pole-to-pole oscillator that controls the spatial assembly of the division machinery in Escherichia coli cells...
  46. Akerlund T, Nordstrom K, Bernander R. Branched Escherichia coli cells. Mol Microbiol. 1993;10:849-58 pubmed
    ..These were found in strains in which chromosome replication or nucleoid segregation was disturbed, e.g. in minB mutants, intR1 strains, and in strains exhibiting stable DNA replication...
  47. Zhou P, Bogan J, Welch K, Pickett S, Wang H, Zaritsky A, et al. Gene transcription and chromosome replication in Escherichia coli. J Bacteriol. 1997;179:163-9 pubmed
    ..The transcriptional levels for two genes, minE and tus, did not fluctuate significantly, whereas the transcripts for one gene, iciA, appeared to increase ..
  48. Yu X, Margolin W. FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localization. Mol Microbiol. 1999;32:315-26 pubmed
    ..A model is proposed in which both the inhibitory effect of the nucleoid and the regulation by MinCDE ensure that cells divide precisely at the midpoint. ..
  49. Norris V. Phospholipid domains determine the spatial organization of the Escherichia coli cell cycle: the membrane tectonics model. J Theor Biol. 1992;154:91-107 pubmed
    ..Escherichia coli will also divide at its poles to generate minicells following overproduction of the FtsZ or MinE proteins. The mechanisms underlying the division inhibition and the positioning of the division sites are unknown...
  50. Sun Q, Margolin W. Influence of the nucleoid on placement of FtsZ and MinE rings in Escherichia coli. J Bacteriol. 2001;183:1413-22 pubmed
    ..Because MinE may protect FtsZ from the action of the MinC inhibitor in these nucleoid-free zones, we examined the localization ..
  51. Pichoff S, Lutkenhaus J. Escherichia coli division inhibitor MinCD blocks septation by preventing Z-ring formation. J Bacteriol. 2001;183:6630-5 pubmed publisher
    ..These results are consistent with FtsZ as the target of MinCD and confirm that this inhibitor prevents Z-ring assembly...
  52. Margolin W. Spatial regulation of cytokinesis in bacteria. Curr Opin Microbiol. 2001;4:647-52 pubmed
    ..This review summarizes and integrates these insights...
  53. Kretschmer S, Zieske K, Schwille P. Large-scale modulation of reconstituted Min protein patterns and gradients by defined mutations in MinE's membrane targeting sequence. PLoS ONE. 2017;12:e0179582 pubmed publisher
    ..These oscillations appeared to require direct membrane interaction of the ATPase activating protein MinE. However, it remained unclear how exactly Min protein dynamics are regulated by MinE membrane binding...
  54. Jaffe A, Boye E, D Ari R. Rule governing the division pattern in Escherichia coli minB and wild-type filaments. J Bacteriol. 1990;172:3500-2 pubmed
    Escherichia coli minB mutants form anucleate minicells and multinucleate filaments. We show here that the overwhelming majority of nucleate cells contain 2n (n = 0, 1, 2, .....
  55. Bernhardt T, de Boer P. Screening for synthetic lethal mutants in Escherichia coli and identification of EnvC (YibP) as a periplasmic septal ring factor with murein hydrolase activity. Mol Microbiol. 2004;52:1255-69 pubmed
    ..By uncovering genetic interactions, the synthetic lethal screen described here provides an attractive new tool for studying gene function in E. coli. ..
  56. Autret S, Errington J. Dynamic proteins in bacteria. Dev Cell. 2001;1:10-1 pubmed
    The MinCDE system regulates the position of the division plane in rod-shaped bacteria. New results from Escherichia coli provide insight into how this operates by showing that MinE stimulates the ATPase activity of MinD.
  57. Aldridge C, Møller S. The plastid division protein AtMinD1 is a Ca2+-ATPase stimulated by AtMinE1. J Biol Chem. 2005;280:31673-8 pubmed
    ..In Escherichia coli precise Z-ring placement at midcell depends on controlled oscillatory behavior of MinD and MinE: In the presence of ATP MinD interacts with the FtsZ inhibitor MinC and migrates to the membrane where the MinD-..
  58. Margolin W. Bacterial cell division: a moving MinE sweeper boggles the MinD. Curr Biol. 2001;11:R395-8 pubmed
    ..Recent studies have shown that MinE, previously thought to form a static ring near the division site at the midcell position, actually joins MinC and ..
  59. Donachie W. Co-ordinate regulation of the Escherichia coli cell cycle or The cloud of unknowing. Mol Microbiol. 2001;40:779-85 pubmed
    ..A discussion of some aspects of the regulation of chromosome replication, segregation and cell division in Escherichia coli. ..
  60. Zhang Y, Rowland S, King G, Braswell E, Rothfield L. The relationship between hetero-oligomer formation and function of the topological specificity domain of the Escherichia coli MinE protein. Mol Microbiol. 1998;30:265-73 pubmed
    b>MinE is an oligomeric protein that, in conjunction with other Min proteins, is required for the proper placement of the cell division site of Escherichia coli...
  61. Akerlund T, Gullbrand B, Nordstr m K. Effects of the Min system on nucleoid segregation in Escherichia coli. Microbiology. 2002;148:3213-22 pubmed publisher
    ..by a mechanism that involves the dynamic localization of all of its three constituent proteins, MinC, MinD and MinE. Both the Min system and the nucleoid regulate cell division negatively and strains of E...
  62. Maple J, Chua N, M ller S. The topological specificity factor AtMinE1 is essential for correct plastid division site placement in Arabidopsis. Plant J. 2002;31:269-77 pubmed
    ..Here we demonstrate that AtMinE1, an Arabidopsis homologue of the bacterial MinE topological specificity factor, is an essential integral component of the plastid division machinery...
  63. Dassain M, Bouche J. The min locus, which confers topological specificity to cell division, is not involved in its coupling with nucleoid separation. J Bacteriol. 1994;176:6143-5 pubmed
    ..We suggest that the retarded nucleoid segregation observed in min mutants is the result of this coupling in cells with a perturbed pattern of nonpolar divisions. ..
  64. Fischer Friedrich E, Meacci G, Lutkenhaus J, Chaté H, Kruse K. Intra- and intercellular fluctuations in Min-protein dynamics decrease with cell length. Proc Natl Acad Sci U S A. 2010;107:6134-9 pubmed publisher
    ..As the pattern becomes more regular, cell-to-cell variability also lessens, indicating cell length-dependent regulation of Min-protein activity. ..
  65. Davie E, Sydnor K, Rothfield L. Genetic basis of minicell formation in Escherichia coli K-12. J Bacteriol. 1984;158:1202-3 pubmed
    ..experiments failed to confirm the presence of a " minA " gene in Escherichia coli K-12, leading to the conclusion that mutation at a single locus, the minB locus, is sufficient to cause minicell production in this species.
  66. Mulder E, El Bouhali M, Pas E, Woldringh C. The Escherichia coli minB mutation resembles gyrB in defective nucleoid segregation and decreased negative supercoiling of plasmids. Mol Gen Genet. 1990;221:87-93 pubmed
    Nucleoid segregation in the Escherichia coli minB mutant and in cells that over-produce minB gene products appeared defective as measured from fluorescence micrographs...
  67. Layton J, Foster P. Error-prone DNA polymerase IV is regulated by the heat shock chaperone GroE in Escherichia coli. J Bacteriol. 2005;187:449-57 pubmed
    ..We were unable to show that GroE interacts directly with Pol IV, suggesting that GroE may act indirectly. Together with previous results, these findings indicate that Pol IV is a component of several cellular stress responses. ..
  68. Barak I, Wilkinson A. Division site recognition in Escherichia coli and Bacillus subtilis. FEMS Microbiol Rev. 2007;31:311-26 pubmed
    ..It also describes how the asymmetric septum forms and how this septation event is linked to chromosome segregation and subsequent asymmetric gene expression during spore formation in B. subtilis. ..
  69. Ivanov V, Mizuuchi K. Multiple modes of interconverting dynamic pattern formation by bacterial cell division proteins. Proc Natl Acad Sci U S A. 2010;107:8071-8 pubmed publisher
    ..is controlled by counteraction of initiation and dissociation complexes; the binding of MinD/E is stimulated by MinE and involves polymerization-depolymerization dynamics; polymerization of MinE over MinD oligomers triggers dynamic ..