melB

Summary

Gene Symbol: melB
Description: melibiose:sodium symporter
Alias: ECK4113, JW4081, mel-4
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Pourcher T, Bibi E, Kaback H, Leblanc G. Membrane topology of the melibiose permease of Escherichia coli studied by melB-phoA fusion analysis. Biochemistry. 1996;35:4161-8 pubmed
    In order to study the secondary structure of the melibiose permease of Escherichia coli, 57 melB-phoA gene fusions were constructed and assayed for alkaline phosphatase activity...
  2. Ding P, Botfield M, Wilson T. Sugar recognition mutants of the melibiose carrier of Escherichia coli: possible structural information concerning the arrangement of membrane-bound helices and sugar/cation recognition site. Biochim Biophys Acta. 2000;1509:123-30 pubmed
    ..The amino acids causing these mutations were determined by sequencing the melB gene on the plasmid. The mutations were located on helices I, IV, VII, X and XI...
  3. Reizer J, Reizer A, Saier M. A functional superfamily of sodium/solute symporters. Biochim Biophys Acta. 1994;1197:133-66 pubmed
    ..This study serves to clarify structural, functional and evolutionary relationships among eleven distinct families of functionally related transport proteins. ..
  4. Hacksell I, Rigaud J, Purhonen P, Pourcher T, Hebert H, Leblanc G. Projection structure at 8 A resolution of the melibiose permease, an Na-sugar co-transporter from Escherichia coli. EMBO J. 2002;21:3569-74 pubmed
    The ion-coupled sugar membrane symporter or co-transporter melibiose permease (MelB), responsible for alpha-galactoside accumulation in Escherichia coli, is a representative member of the glycoside-pentoside- hexuronide family of the vast ..
  5. Maehrel C, Cordat E, Mus Veteau I, Leblanc G. Structural studies of the melibiose permease of Escherichia coli by fluorescence resonance energy transfer. I. Evidence for ion-induced conformational change. J Biol Chem. 1998;273:33192-7 pubmed
    Further insight into the cosubstrate-induced structural change of the melibiose permease (MelB) of Escherichia coli has been sought by investigating the binding and spectroscopic properties of the fluorescent sugar 2'-(N-5-..
  6. Grainger D, Overton T, Reppas N, Wade J, Tamai E, Hobman J, et al. Genomic studies with Escherichia coli MelR protein: applications of chromatin immunoprecipitation and microarrays. J Bacteriol. 2004;186:6938-43 pubmed
    ..coli for MelR. This was confirmed by a transcriptomics experiment to analyze RNA in cells either with or without melR. ..
  7. Roepe P, Kaback H. Isolation and functional reconstitution of soluble melibiose permease from Escherichia coli. Biochemistry. 1990;29:2572-7 pubmed
    ..integral plasma membrane protein, has been purified in a metastable soluble form after overexpression of the melB gene via the T7 RNA polymerase system...
  8. Ganea C, Fendler K. Bacterial transporters: charge translocation and mechanism. Biochim Biophys Acta. 2009;1787:706-13 pubmed publisher
    ..In melibiose permease MelB and the Na(+)/proline carrier PutP pre-steady-state charge displacements can be assigned to an electrogenic ..
  9. Wilson D, Wilson T. Cation specificity for sugar substrates of the melibiose carrier in Escherichia coli. Biochim Biophys Acta. 1987;904:191-200 pubmed
    ..The sugar specificity requirements for H+ cotransport are therefore more exacting than those for Na+ cotransport. ..

More Information

Publications49

  1. Wilson D, Wilson T. Asp-51 and Asp-120 are important for the transport function of the Escherichia coli melibiose carrier. J Bacteriol. 1992;174:3083-6 pubmed
    ..Revertants to Glu-51 and Glu-120 show less activity than the wild type. The Asp-51 position is more crucial for Na(+)-stimulated melibiose accumulation than is the Asp-120 site. ..
  2. Ambroise Y, Leblanc G, Rousseau B. Active-site-directed photolabeling of the melibiose permease of Escherichia coli. Biochemistry. 2000;39:1338-45 pubmed
    Covalent photolabeling of the melibiose permease (MelB) of Escherichia coli has been undertaken with the sugar analogue [(3)H]-p-azidophenyl alpha-D-galactopyranoside ([(3)H]-alpha-PAPG) with the purpose of identifying the domains forming ..
  3. Pourcher T, Leclercq S, Brandolin G, Leblanc G. Melibiose permease of Escherichia coli: large scale purification and evidence that H+, Na+, and Li+ sugar symport is catalyzed by a single polypeptide. Biochemistry. 1995;34:4412-20 pubmed
    ..Mel-6His permease was constructed by modifying the permease gene (melB) in vitro and then overproduced in cells transformed with multicopy plasmids...
  4. Botfield M, Wilson D, Wilson T. The melibiose carrier of Escherichia coli. Res Microbiol. 1990;141:328-31 pubmed
  5. Meyer Lipp K, Ganea C, Pourcher T, Leblanc G, Fendler K. Sugar binding induced charge translocation in the melibiose permease from Escherichia coli. Biochemistry. 2004;43:12606-13 pubmed
    Electrogenic events associated with the activity of the melibiose permease (MelB), a transporter from Escherichia coli, were investigated...
  6. Bassilana M, Gwizdek C. In vivo membrane assembly of the E.coli polytopic protein, melibiose permease, occurs via a Sec-independent process which requires the protonmotive force. EMBO J. 1996;15:5202-8 pubmed
    ..for in vivo membrane assembly of the prototypic 12 transmembrane domain sugar co-transporter, melibiose permease (MelB)...
  7. Bourgerie S, Michán C, Thomas M, Busby S, Hyde E. DNA binding and DNA bending by the MelR transcription activator protein from Escherichia coli. Nucleic Acids Res. 1997;25:1685-93 pubmed
    ..Bound full-length MelR, but not MelR173, can oligomerise to form larger complexes that are likely to be involved in transcription activation. ..
  8. Mus Veteau I, Leblanc G. Melibiose permease of Escherichia coli: structural organization of cosubstrate binding sites as deduced from tryptophan fluorescence analyses. Biochemistry. 1996;35:12053-60 pubmed
    ..This implies close proximity and/or tight functional linkage between some N-terminal helices and helices IX and X of the C-terminal domain of the transporter. ..
  9. Cordat E, Mus Veteau I, Leblanc G. Structural studies of the melibiose permease of Escherichia coli by fluorescence resonance energy transfer. II. Identification of the tryptophan residues acting as energy donors. J Biol Chem. 1998;273:33198-202 pubmed
    ..the presence of a fluorescence resonance energy transfer (FRET) between the tryptophans of the melibiose permease (MelB) of Escherichia coli and a fluorescent sugar, 2'-(N-5-dimethylaminonaphthalene-1-sulfonyl)aminoethyl-1-thio-beta-D- ..
  10. Hariharan P, Guan L. Insights into the inhibitory mechanisms of the regulatory protein IIA(Glc) on melibiose permease activity. J Biol Chem. 2014;289:33012-9 pubmed publisher
    ..Melibiose permease (MelB) is one among several permeases subject to IIA(Glc) regulation...
  11. Yousef M, Guan L. A 3D structure model of the melibiose permease of Escherichia coli represents a distinctive fold for Na+ symporters. Proc Natl Acad Sci U S A. 2009;106:15291-6 pubmed publisher
    The melibiose permease of Escherichia coli (MelB) catalyzes the coupled stoichiometric symport of a galactoside with a cation (either Na(+), Li(+), or H(+)), using free energy from the downhill translocation of one cosubstrate to catalyze ..
  12. Leon X, Lemonnier R, Leblanc G, Padrós E. Changes in secondary structures and acidic side chains of melibiose permease upon cosubstrates binding. Biophys J. 2006;91:4440-9 pubmed
    ..The latter carboxyl groups may correspond to part or all of the acidic residues interacting with Lys or Arg in the resting state that become free upon melibiose binding. ..
  13. Dave N, Lorenz Fonfria V, Leblanc G, Padrós E. FTIR spectroscopy of secondary-structure reorientation of melibiose permease modulated by substrate binding. Biophys J. 2008;94:3659-70 pubmed
    ..Notably, cation binding essentially influences type I helices, whereas melibiose binding modifies the tilting of both helix populations. ..
  14. Purhonen P, Lundbäck A, Lemonnier R, Leblanc G, Hebert H. Three-dimensional structure of the sugar symporter melibiose permease from cryo-electron microscopy. J Struct Biol. 2005;152:76-83 pubmed
    Melibiose permease (MelB) of Escherichia coli is a secondary transporter that couples the uptake of melibiose and various other galactosides to symport of cations that can be Na+, Li+ or H+...
  15. Grainger D, Belyaeva T, Lee D, Hyde E, Busby S. Transcription activation at the Escherichia coli melAB promoter: interactions of MelR with the C-terminal domain of the RNA polymerase alpha subunit. Mol Microbiol. 2004;51:1311-20 pubmed
    ..Finally, we used alanine scanning to identify determinants in the C-terminal domain of the RNA polymerase alpha subunit that are important for MelR-dependent activation of the melAB promoter. ..
  16. Dumas F, Tocanne J, Leblanc G, Lebrun M. Consequences of hydrophobic mismatch between lipids and melibiose permease on melibiose transport. Biochemistry. 2000;39:4846-54 pubmed
    ..a transmembrane protein and that d(L) of the supporting lipid bilayer were investigated using melibiose permease (MelB) from Escherichia coli reconstituted in a set of bis saturated and monounsaturated phosphatidylcholine species ..
  17. Kahramanoglou C, Webster C, El Robh M, Belyaeva T, Busby S. Mutational analysis of the Escherichia coli melR gene suggests a two-state concerted model to explain transcriptional activation and repression in the melibiose operon. J Bacteriol. 2006;188:3199-207 pubmed
    ..Additional experiments with a bacterial two-hybrid system suggest that interactions between MelR subunits differ according to the two conformational states. ..
  18. Fuerst O, Lin Y, Granell M, Leblanc G, Padrós E, Lórenz Fonfría V, et al. The Melibiose Transporter of Escherichia coli: CRITICAL CONTRIBUTION OF LYS-377 TO THE STRUCTURAL ORGANIZATION OF THE INTERACTING SUBSTRATE BINDING SITES. J Biol Chem. 2015;290:16261-71 pubmed publisher
    ..This amino acid is located in the border of the sugar-binding site and might participate in sugar binding through apolar interactions. ..
  19. Pourcher T, Deckert M, Bassilana M, Leblanc G. Melibiose permease of Escherichia coli: mutation of aspartic acid 55 in putative helix II abolishes activation of sugar binding by Na+ ions. Biochem Biophys Res Commun. 1991;178:1176-81 pubmed
  20. Ganea C, Pourcher T, Leblanc G, Fendler K. Evidence for intraprotein charge transfer during the transport activity of the melibiose permease from Escherichia coli. Biochemistry. 2001;40:13744-52 pubmed
    Electrogenic activity associated with the activity of the melibiose permease (MelB) of Escherichia coli was investigated by using proteoliposomes containing purified MelB adsorbed onto a solid-supported membrane...
  21. Dave N, Lorenz Fonfria V, Villaverde J, Lemonnier R, Leblanc G, Padrós E. Study of amide-proton exchange of Escherichia coli melibiose permease by attenuated total reflection-Fourier transform infrared spectroscopy: evidence of structure modulation by substrate binding. J Biol Chem. 2002;277:3380-7 pubmed
    ..by attenuated total reflection-Fourier transform infrared spectroscopy under four distinct conditions where MelB forms different complexes with its substrates (H(+), Na(+), melibiose)...
  22. Grainger D, Belyaeva T, Lee D, Hyde E, Busby S. Binding of the Escherichia coli MelR protein to the melAB promoter: orientation of MelR subunits and investigation of MelR-DNA contacts. Mol Microbiol. 2003;48:335-48 pubmed
    ..Epistasis experiments provided evidence that supports the proposed orientation of binding of MelR at its target site. ..
  23. Ding P. Loop X/XI, the largest cytoplasmic loop in the membrane-bound melibiose carrier of Escherichia coli, is a functional re-entrant loop. Biochim Biophys Acta. 2004;1660:106-17 pubmed
    ..Together, these results strongly indicated a functional re-entrant loop mechanistically important in Na+-coupled transporters. ..
  24. Bassilana M, Pourcher T, Leblanc G. Facilitated diffusion properties of melibiose permease in Escherichia coli membrane vesicles. Release of co-substrates is rate limiting for permease cycling. J Biol Chem. 1987;262:16865-70 pubmed
    ..Finally, evidence is provided suggesting that the relative rates of release of the two co-substrates from the permease on the inner membrane surface varied considerably in relation to the identity of the coupled cation. ..
  25. Tamai E, Shimamoto T, Tsuda M, Mizushima T, Tsuchiya T. Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli. J Biol Chem. 1998;273:16860-4 pubmed
    ..The reason for this temperature sensitivity was thought to be that the melibiose transporter (MelB) of W3133 cells was temperature-sensitive...
  26. Gwizdek C, Leblanc G, Bassilana M. Proteolytic mapping and substrate protection of the Escherichia coli melibiose permease. Biochemistry. 1997;36:8522-9 pubmed
    The topology and substrate-induced conformational change(s) of the Na+ (Li+ or H+)-melibiose cotransporter (MelB) of Escherichia coli were investigated by limited protease digestion...
  27. Keen J, Williams J, Busby S. Location of essential sequence elements at the Escherichia coli melAB promoter. Biochem J. 1996;318 ( Pt 2):443-9 pubmed
    ..The results show that the organization of the melAB promoter is fundamentally different from the organization of other bacterial promoters controlled by homologues of MelR. ..
  28. Yazyu H, Shiota Niiya S, Shimamoto T, Kanazawa H, Futai M, Tsuchiya T. Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J Biol Chem. 1984;259:4320-6 pubmed
    The nucleotide sequence of the melB gene coding for the melibiose carrier in Escherichia coli has been determined...
  29. Ding P, Wilson T. The effect of modifications of the charged residues in the transmembrane helices on the transport activity of the melibiose carrier of Escherichia coli. Biochem Biophys Res Commun. 2001;285:348-54 pubmed
    The melibiose transport carrier of Escherichia coli (coded by melB gene) is a cotransport system which couples the transport of a-galactosides to protons, sodium, or lithium ions...
  30. Leon X, Leblanc G, Padrós E. Alteration of sugar-induced conformational changes of the melibiose permease by mutating Arg141 in loop 4-5. Biophys J. 2009;96:4877-86 pubmed publisher
    The melibiose permease (MelB) from Escherichia coli couples the uptake of melibiose to that of Na+, Li+, or H+...
  31. Garcia Celma J, Dueck B, Stein M, Schlueter M, Meyer Lipp K, Leblanc G, et al. Rapid activation of the melibiose permease MelB immobilized on a solid-supported membrane. Langmuir. 2008;24:8119-26 pubmed publisher
    ..As an application of the technique, the rate constant of a fast electrogenic reaction in the melibiose permease MelB, a bacterial ( Escherichia coli) sugar transporter, is determined...
  32. Hanatani M, Yazyu H, Shiota Niiya S, Moriyama Y, Kanazawa H, Futai M, et al. Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli. J Biol Chem. 1984;259:1807-12 pubmed
    ..Genetic complementation tests and recombination analyses using those plasmids and melA- and melB- mutants as well as biochemical analyses of mel mutants transformed with those plasmids enabled us to determine the ..
  33. Meyer Lipp K, Séry N, Ganea C, Basquin C, Fendler K, Leblanc G. The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding. J Biol Chem. 2006;281:25882-92 pubmed
    ..Biochemistry 43, 12606-12613), were much smaller for the two mutants than the corresponding signals in cysteineless MelB. Furthermore, in R141C the stimulating effect of melibiose on Na+ affinity was lost...
  34. Leon X, Lorenz Fonfria V, Lemonnier R, Leblanc G, Padrós E. Substrate-induced conformational changes of melibiose permease from Escherichia coli studied by infrared difference spectroscopy. Biochemistry. 2005;44:3506-14 pubmed
    ..has been used to obtain information about substrate-induced structural changes of the melibiose permease (MelB) from Escherichia coli reconstituted into liposomes...
  35. Hastings Wilson T, Wilson D. Evidence for a close association between helix IV and helix XI in the melibiose carrier of Escherichia coli. Biochim Biophys Acta. 1998;1374:77-82 pubmed
    ..of the carrier protein was replaced with Ser, Ile or Phe by site-directed mutagenesis of a plasmid containing the melB gene...
  36. Wilson T, Ding P. Sodium-substrate cotransport in bacteria. Biochim Biophys Acta. 2001;1505:121-30 pubmed
    ..Other sodium cotransport carriers that have been studied recently are for proline, glutamate, serine-threonine, citrate and branched chain amino acids. ..
  37. Ding P. An investigation of cysteine mutants on the cytoplasmic loop X/XI in the melibiose transporter of Escherichia coli by using thiol reagents: implication of structural conservation of charged residues. Biochem Biophys Res Commun. 2003;307:864-9 pubmed
  38. Giffard P, Booth I. The rpoA341 allele of Escherichia coli specifically impairs the transcription of a group of positively-regulated operons. Mol Gen Genet. 1988;214:148-52 pubmed
    ..The significance of these observations is discussed in the context of models of positive regulation. ..
  39. Yazyu H, Shiota S, Futai M, Tsuchiya T. Alteration in cation specificity of the melibiose transport carrier of Escherichia coli due to replacement of proline 122 with serine. J Bacteriol. 1985;162:933-7 pubmed
    The structural genes (melB) for the melibiose carrier of five mutants of Escherichia coli showing altered cation specificity for melibiose transport were cloned...
  40. Botfield M, Naguchi K, Tsuchiya T, Wilson T. Membrane topology of the melibiose carrier of Escherichia coli. J Biol Chem. 1992;267:1818-22 pubmed
    ..Microbiol. 141, 374-383). Such a broad degree of conservation (or convergence) suggests a distinct structural and/or mechanistic advantage associated with the six-loop-six motif. The nature of this advantage is as yet unknown. ..