maeB

Summary

Gene Symbol: maeB
Description: malic enzyme: putative oxidoreductase/phosphotransacetylase
Alias: ECK2458, JW2447, ypfF
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Iwakura M, Tokushige M, Katsuki H. Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli W. J Biochem. 1979;86:1239-49 pubmed
    ..The protective effect of NADP+ was increased by the addition of Mg2+. Based on the above results, the effects of Mg2+ on the SH-group modification are discussed from the viewpoint of conformational alteration of the enzyme. ..
  2. Mahajan S, Chu C, Willis D, Templin A, Clark A. Physical analysis of spontaneous and mutagen-induced mutants of Escherichia coli K-12 expressing DNA exonuclease VIII activity. Genetics. 1990;125:261-73 pubmed
    ..These are presumed point mutations. Three seem to produce additional restriction nucleotide fragments complementary to lambda rev. The possible nature of these sbc mutations is discussed. ..
  3. van der Rest M, Frank C, Molenaar D. Functions of the membrane-associated and cytoplasmic malate dehydrogenases in the citric acid cycle of Escherichia coli. J Bacteriol. 2000;182:6892-9 pubmed
    ..coli. However, comparing growth of the mdh single mutant to that of the double mutant containing mdh and mqo deletions did indicate that MQO partly takes over the function of MDH in an mdh mutant. ..
  4. Tokushige M, Hattori J, Katsuki H. Distinct effects of pyridoxal phosphate on NAD- and NADP- linked malic enzymes of Escherichia coli. Physiol Chem Phys Med NMR. 1985;17:347-50 pubmed
    ..In contrast, NAD-linked malic enzyme from the same micro-organism was insensitive to PLP, even in the presence of 0.8 M guanidine hydrochloride. ..
  5. Sanwal B, Smando R. Regulatory roles of cyclic 3',5'-AMP in bacteria: control of malic enzyme of Escherichia coli. Biochem Biophys Res Commun. 1969;35:486-91 pubmed
  6. Sanwal B. Regulatory characteristics of the diphosphopyridine nucleotide-specific malic enzyme of Escherichia coli. J Biol Chem. 1970;245:1212-6 pubmed
  7. Siddiquee K, Arauzo Bravo M, Shimizu K. Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli. FEMS Microbiol Lett. 2004;235:25-33 pubmed
    ..RT-PCR revealed that the glycolytic genes such as glk, pgi, pfkA and tpiA were down regulated, that ppc, pckA, maeB and mdh genes were strongly up-regulated, and that the oxidative pentose phosphate pathway genes such as zwf and ..
  8. Shin J, Kwon Y, Kwon O, Lee H, Kim P. 5-aminolevulinic acid biosynthesis in Escherichia coli coexpressing NADP-dependent malic enzyme and 5-aminolevulinate synthase. J Microbiol Biotechnol. 2007;17:1579-84 pubmed
    ..To study the effect of the enhanced C4 metabolism of E. coli on ALA biosynthesis, NADP-dependent malic enzyme (maeB, E.C. 1.1.1.40) was coexpressed with ALA synthase in E. coli...
  9. Sanwal B, Wright J, Smando R. Allosteric control of the activity of malic enzyme in Escherichia coli. Biochem Biophys Res Commun. 1968;31:623-7 pubmed

More Information

Publications24

  1. Iwakura M, Hattori J, Arita Y, Tokushige M, Katsuki H. Studies on regulatory functions of malic enzymes. VI. Purification and molecular properties of NADP-linked malic enzyme from Escherichia coli W. J Biochem. 1979;85:1355-65 pubmed
    ..Based on the above results, the molecular properties of the enzymatic reaction are discussed. ..
  2. Sanwal B, Smando R. Malic enzyme of Escherichia coli. Possible mechanism for allosteric effects. J Biol Chem. 1969;244:1824-30 pubmed
  3. Murai T, Tokushige M, Nagai J, Katsuki H. Studies on regulatory functions of malic enzymes. I. Metabolic functions of NAD- and NADP-linked malic enzymes in Escherichia coli. J Biochem. 1972;71:1015-28 pubmed
  4. Brown D, Cook R. Role of metal cofactors in enzyme regulation. Differences in the regulatory properties of the Escherichia coli nicotinamide adenine dinucleotide phosphate specific malic enzyme, depending on whether magnesium ion or manganese ion serves as divalent c. Biochemistry. 1981;20:2503-12 pubmed
  5. Brown D, Cook R. Regulatory effects of potassium and inorganic anions on the NADP-specific malic enzyme of Escherichia coli. Can J Biochem Cell Biol. 1985;63:128-36 pubmed
    ..The kinetic studies conducted at higher ionic strength in vitro are therefore more applicable to the in vivo situation. ..
  6. Takeo K. Existence and properties of two malic enzymes in Escherichia coli especially of NAD-linked enzyme. J Biochem. 1969;66:379-87 pubmed
  7. Katsuki H, Takeo K, Kameda K, Tanaka S. Existence of two malic enzymes in Escherichia coli. Biochem Biophys Res Commun. 1967;27:331-6 pubmed
  8. Trinh C, Carlson R, Wlaschin A, Srienc F. Design, construction and performance of the most efficient biomass producing E. coli bacterium. Metab Eng. 2006;8:628-38 pubmed
    ..The results show that the theoretical predictions are closely matched by the properties of the designed strain. ..
  9. Wang B, Wang P, Zheng E, Chen X, Zhao H, Song P, et al. Biochemical properties and physiological roles of NADP-dependent malic enzyme in Escherichia coli. J Microbiol. 2011;49:797-802 pubmed publisher
    ..NADP-dependent malic enzyme (MaeB) from Escherichia coli MG1655 was expressed and purified as a fusion protein...
  10. Takeo K, Murai T, Nagai J, Katsuki H. Allosteric activation of DPN-linked malic enzyme from Escherichia coli by aspartate. Biochem Biophys Res Commun. 1967;29:717-22 pubmed
  11. Kao K, Tran L, Liao J. A global regulatory role of gluconeogenic genes in Escherichia coli revealed by transcriptome network analysis. J Biol Chem. 2005;280:36079-87 pubmed
    ..This work demonstrates that the gluconeogenic genes in Escherichia coli, ppsA, sfcA, and maeB, provide a feedback loop to the global regulator, cAMP receptor protein (CRP), in carbon source transition...
  12. Murai T, Tokushige M, Nagai J, Katsuki H. Physiological functions of NAD- and NADP-linked malic enzymes in Escherichia coli. Biochem Biophys Res Commun. 1971;43:875-81 pubmed
  13. Bologna F, Andreo C, Drincovich M. Escherichia coli malic enzymes: two isoforms with substantial differences in kinetic properties, metabolic regulation, and structure. J Bacteriol. 2007;189:5937-46 pubmed
    ..In Escherichia coli, two genes with a high degree of homology to ME can be detected: sfcA and maeB. MaeB possesses a multimodular structure: the N-terminal extension shows homology to ME, while the C-terminal ..
  14. Sanwal B, Smando R. Malic enzyme of Escherichia coli. Diversity of the effectors controlling enzyme activity. J Biol Chem. 1969;244:1817-23 pubmed
  15. Kwon Y, Kwon O, Lee H, Kim P. The effect of NADP-dependent malic enzyme expression and anaerobic C4 metabolism in Escherichia coli compared with other anaplerotic enzymes. J Appl Microbiol. 2007;103:2340-5 pubmed
    ..Anaplerotic NADP-dependent malic enzyme (MaeB), as well as the other anaplerotic enzymes, including phosphoenolpyruvate carboxylase (Ppc), phosphoenolpyruvate ..