Gene Symbol: macB
Description: macrolide ABC transporter peremase/ATPase
Alias: ECK0870, JW0863, ybjZ
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Kobayashi N, Nishino K, Yamaguchi A. Novel macrolide-specific ABC-type efflux transporter in Escherichia coli. J Bacteriol. 2001;183:5639-44 pubmed
    ..Neither of the two genes produced resistance alone. The DNA sequence suggests that MacB is an integral membrane protein with four transmembrane segments and one nucleotide-binding domain, while MacA ..
  2. Kobayashi N, Nishino K, Hirata T, Yamaguchi A. Membrane topology of ABC-type macrolide antibiotic exporter MacB in Escherichia coli. FEBS Lett. 2003;546:241-6 pubmed
    b>MacB is an ABC-type membrane protein that exports only macrolide compounds containing 14- and 15-membered lactones, cooperating with a membrane fusion protein, MacA, and a multifunctional outer membrane channel, TolC...
  3. Tikhonova E, Devroy V, Lau S, Zgurskaya H. Reconstitution of the Escherichia coli macrolide transporter: the periplasmic membrane fusion protein MacA stimulates the ATPase activity of MacB. Mol Microbiol. 2007;63:895-910 pubmed
    ..Here, MacA is a periplasmic MFP and MacB is an ABC-type transporter. Similar to other MFP-dependent transporters from E...
  4. Kobayashi K, Tsukagoshi N, Aono R. Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element. J Bacteriol. 2001;183:2646-53 pubmed
    ..Thus, it is concluded that in these cells a combination of the proteins AcrA, AcrF, and TolC or the proteins AcrE, AcrF, and TolC is functional in solvent efflux instead of the AcrAB-TolC efflux pump. ..
  5. Yamada S, Awano N, Inubushi K, Maeda E, Nakamori S, Nishino K, et al. Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli. Appl Environ Microbiol. 2006;72:4735-42 pubmed
    ..These results indicate that the multidrug transporter Bcr in the major facilitator family is involved in L-cysteine export and overproduction in genetically engineered E. coli cells. ..
  6. Lin H, Bavro V, Barrera N, Frankish H, Velamakanni S, van Veen H, et al. MacB ABC transporter is a dimer whose ATPase activity and macrolide-binding capacity are regulated by the membrane fusion protein MacA. J Biol Chem. 2009;284:1145-54 pubmed publisher
    ..fusion protein MacA not only stabilizes the tripartite assembly by interacting with both the inner membrane protein MacB and the outer membrane protein TolC, but also has a role in regulating the function of MacB, apparently increasing ..
  7. Modali S, Zgurskaya H. The periplasmic membrane proximal domain of MacA acts as a switch in stimulation of ATP hydrolysis by MacB transporter. Mol Microbiol. 2011;81:937-51 pubmed publisher
    ..In this complex, MacA is the periplasmic membrane fusion protein that stimulates the activity of MacB transporter and establishes the link with the outer membrane channel TolC...
  8. Turlin E, Heuck G, Simões Brandão M, Szili N, Mellin J, Lange N, et al. Protoporphyrin (PPIX) efflux by the MacAB-TolC pump in Escherichia coli. Microbiologyopen. 2014;3:849-59 pubmed publisher
    ..coli and S. typhimurium and that this compound is produced inside bacteria when natural heme homeostasis is disrupted by iron shortage, as happens when bacteria invade the mammalian host. ..
  9. Yamanaka N, Yamamoto Y, Kuki K. Engraftment of tonsillar mononuclear cells in human skin/SCID mouse chimera--validation of a novel xenogeneic transplantation model for autoimmune diseases. Microbiol Immunol. 2001;45:507-14 pubmed
    ..In this review, we addressed the existing deficiencies in our understanding of the relationship between tonsils and PPP by using emerging transplantation technology involving SCID mice. ..

More Information


  1. Jo I, Hong S, Lee M, Song S, Kim J, Mitra A, et al. Stoichiometry and mechanistic implications of the MacAB-TolC tripartite efflux pump. Biochem Biophys Res Commun. 2017;494:668-673 pubmed publisher
    ..Nonetheless, the inner membrane transporter MacB still remains ambiguous in terms of its oligomeric state in the functional complex...