Gene Symbol: macA
Description: macrolide transporter membrane fusion protein (MFP) component
Alias: ECK0869, JW0862, ybjY
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Kobayashi N, Nishino K, Yamaguchi A. Novel macrolide-specific ABC-type efflux transporter in Escherichia coli. J Bacteriol. 2001;183:5639-44 pubmed
    ..Therefore, ybjYZ was renamed macAB (macrolide-specific ABC-type efflux carrier). Plasmids carrying both the macA and -B genes conferred resistance against macrolides composed of 14- and 15-membered lactones but no or weak ..
  2. Tikhonova E, Devroy V, Lau S, Zgurskaya H. Reconstitution of the Escherichia coli macrolide transporter: the periplasmic membrane fusion protein MacA stimulates the ATPase activity of MacB. Mol Microbiol. 2007;63:895-910 pubmed
    ..In this study, we purified and reconstituted the macrolide transporter MacAB from Escherichia coli. Here, MacA is a periplasmic MFP and MacB is an ABC-type transporter. Similar to other MFP-dependent transporters from E...
  3. Kobayashi K, Tsukagoshi N, Aono R. Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element. J Bacteriol. 2001;183:2646-53 pubmed
    ..Thus, it is concluded that in these cells a combination of the proteins AcrA, AcrF, and TolC or the proteins AcrE, AcrF, and TolC is functional in solvent efflux instead of the AcrAB-TolC efflux pump. ..
  4. Tikhonova E, Dastidar V, Rybenkov V, Zgurskaya H. Kinetic control of TolC recruitment by multidrug efflux complexes. Proc Natl Acad Sci U S A. 2009;106:16416-21 pubmed publisher
    ..In this study, we reconstituted interactions and compared binding kinetics of the E. coli TolC with AcrA, MacA, and EmrA, the periplasmic MFPs that function in multidrug efflux with transporters from the RND, ABC, and MF ..
  5. Xu Y, Sim S, Song S, Piao S, Kim H, Jin X, et al. The tip region of the MacA alpha-hairpin is important for the binding to TolC to the Escherichia coli MacAB-TolC pump. Biochem Biophys Res Commun. 2010;394:962-5 pubmed publisher
    ..We previously reported the funnel-like hexameric structure of the adaptor protein MacA to be physiologically relevant...
  6. Modali S, Zgurskaya H. The periplasmic membrane proximal domain of MacA acts as a switch in stimulation of ATP hydrolysis by MacB transporter. Mol Microbiol. 2011;81:937-51 pubmed publisher
    ..In this complex, MacA is the periplasmic membrane fusion protein that stimulates the activity of MacB transporter and establishes the ..
  7. Yamanaka N, Yamamoto Y, Kuki K. Engraftment of tonsillar mononuclear cells in human skin/SCID mouse chimera--validation of a novel xenogeneic transplantation model for autoimmune diseases. Microbiol Immunol. 2001;45:507-14 pubmed
    ..In this review, we addressed the existing deficiencies in our understanding of the relationship between tonsils and PPP by using emerging transplantation technology involving SCID mice. ..
  8. Yamada S, Awano N, Inubushi K, Maeda E, Nakamori S, Nishino K, et al. Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli. Appl Environ Microbiol. 2006;72:4735-42 pubmed
    ..These results indicate that the multidrug transporter Bcr in the major facilitator family is involved in L-cysteine export and overproduction in genetically engineered E. coli cells. ..
  9. Lin H, Bavro V, Barrera N, Frankish H, Velamakanni S, van Veen H, et al. MacB ABC transporter is a dimer whose ATPase activity and macrolide-binding capacity are regulated by the membrane fusion protein MacA. J Biol Chem. 2009;284:1145-54 pubmed publisher
    ..The membrane fusion protein MacA not only stabilizes the tripartite assembly by interacting with both the inner membrane protein MacB and the outer ..

More Information


  1. Yum S, Xu Y, Piao S, Sim S, Kim H, Jo W, et al. Crystal structure of the periplasmic component of a tripartite macrolide-specific efflux pump. J Mol Biol. 2009;387:1286-97 pubmed publisher
    ..b>MacA is the periplasmic MFP in the MacAB-TolC pump, where MacB was characterized as a macrolide-specific ATP-binding-..
  2. Turlin E, Heuck G, Simões Brandão M, Szili N, Mellin J, Lange N, et al. Protoporphyrin (PPIX) efflux by the MacAB-TolC pump in Escherichia coli. Microbiologyopen. 2014;3:849-59 pubmed publisher
    ..coli and S. typhimurium and that this compound is produced inside bacteria when natural heme homeostasis is disrupted by iron shortage, as happens when bacteria invade the mammalian host. ..
  3. Jo I, Hong S, Lee M, Song S, Kim J, Mitra A, et al. Stoichiometry and mechanistic implications of the MacAB-TolC tripartite efflux pump. Biochem Biophys Res Commun. 2017;494:668-673 pubmed publisher
    ..X-ray crystal and electron microscopic structures have revealed the funnel-like MacA hexamer in the periplasmic space and the cylindrical TolC trimer...