Gene Symbol: lsrK
Description: autoinducer-2 (AI-2) kinase
Alias: ECK1504, JW1504, ydeV
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Xavier K, Bassler B. Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli. J Bacteriol. 2005;187:238-48 pubmed
    ..coli, and screening for mutants defective in AI-2 internalization revealed lsrK and glpD...
  2. Schembri M, Kjaergaard K, Klemm P. Global gene expression in Escherichia coli biofilms. Mol Microbiol. 2003;48:253-67 pubmed
  3. Wang L, Li J, March J, Valdes J, Bentley W. luxS-dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling. J Bacteriol. 2005;187:8350-60 pubmed
    ..Under the latter condition, the metE gene, the lsrACDBFG operon, and the flanking genes of the lsr operon (lsrR, lsrK, tam, and yneE) were among the most significantly induced genes by luxS. The E...
  4. Bansal T, Jesudhasan P, Pillai S, Wood T, Jayaraman A. Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2. Appl Microbiol Biotechnol. 2008;78:811-9 pubmed publisher
    ..Our results suggest that AI-2 is an important signal in EHEC infections of the human GI tract. ..
  5. Zhu J, Yin X, Yu H, Zhao L, Sabour P, Gong J. Involvement of quorum sensing and heat-stable enterotoxin a in cell damage caused by a porcine enterotoxigenic Escherichia coli strain. Infect Immun. 2011;79:1688-95 pubmed publisher
    ..These results suggest the involvement of AI-2-mediated quorum sensing in K88(+) ETEC pathogenesis, possibly through a negative regulation of STa production. ..
  6. Ha J, Eo Y, Ahn H, Ryu K. Increasing the soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK. Acta Crystallogr F Struct Biol Commun. 2017;73:253-258 pubmed publisher
    b>LsrK is one of the key components of the luxS-regulated (lsr) operon in Escherichia coli and plays an important role during the quorum-sensing (QS) process mediated by autoinducer-2 (AI-2)...
  7. Taga M, Miller S, Bassler B. Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium. Mol Microbiol. 2003;50:1411-27 pubmed
    ..In this report, we identify and characterize LsrK, a new protein that is required for the regulation of the lsr operon and the AI-2 uptake process...
  8. Xavier K, Bassler B. Interference with AI-2-mediated bacterial cell-cell communication. Nature. 2005;437:750-3 pubmed
    ..AI-2 signalling, and the interference with it, could have important ramifications for eukaryotes in the maintenance of normal microflora and in protection from pathogenic bacteria. ..
  9. Xavier K, Miller S, Lu W, Kim J, Rabinowitz J, Pelczer I, et al. Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria. ACS Chem Biol. 2007;2:128-36 pubmed
    ..Here we show that following internalization, the kinase LsrK phosphorylates carbon-5 of the open form of DPD...

More Information


  1. Zhang X, GarcĂ­a Contreras R, Wood T. Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA). ISME J. 2008;2:615-31 pubmed publisher
    ..Overexpressing MqsR, AI-2 import regulators LsrR/LsrK and AI-2 exporter TqsA induced yncC transcription, whereas the AI-2 synthase LuxS and B3023 repressed yncC...
  2. Das R, Reddy E, Chatterjee D, Andrews D. Identification of a novel Bcl-2 related gene, BRAG-1, in human glioma. Oncogene. 1996;12:947-51 pubmed
    ..Utilizing a bacterial expression vector, we produced BRAG-1 protein which was found to cross-react with a Bcl-2 monoclonal antibody, further suggesting structural and immunological similarity to Bcl-2. ..
  3. Ahmer B. Cell-to-cell signalling in Escherichia coli and Salmonella enterica. Mol Microbiol. 2004;52:933-45 pubmed
    ..The latter system has homologues in E. coli and Salmonella, as well as other bacteria, plants and animals. In fact, the protease components from Providencia and Drosophila are functionally interchangeable. ..
  4. Li J, Attila C, Wang L, Wood T, Valdes J, Bentley W. Quorum sensing in Escherichia coli is signaled by AI-2/LsrR: effects on small RNA and biofilm architecture. J Bacteriol. 2007;189:6011-20 pubmed
    ..2 (AI-2) is comprised of a transporter complex, LsrABCD; its repressor, LsrR; and a cognate signal kinase, LsrK. This network is an integral part of the AI-2 quorum-sensing (QS) system...
  5. Tavender T, Halliday N, Hardie K, Winzer K. LuxS-independent formation of AI-2 from ribulose-5-phosphate. BMC Microbiol. 2008;8:98 pubmed publisher
    ..It does not contribute to AI-2 formation in wildtype E. coli and S. aureus under the conditions tested, but may be responsible for the AI-2-like activities reported for other organisms lacking the luxS gene. ..
  6. Roy V, Fernandes R, Tsao C, Bentley W. Cross species quorum quenching using a native AI-2 processing enzyme. ACS Chem Biol. 2010;5:223-32 pubmed publisher
    ..We demonstrate that the Escherichia coli AI-2 kinase, LsrK, can phosphorylate AI-2 in vitro, and when LsrK-treated AI-2 is added ex vivo to E...
  7. Wang L, Hashimoto Y, Tsao C, Valdes J, Bentley W. Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli. J Bacteriol. 2005;187:2066-76 pubmed
    ..The functions of the lsr operon and its regulators, LsrR and LsrK, previously reported in Salmonella enterica serovar Typhimurium, are confirmed here for E. coli...