Gene Symbol: lsrF
Description: putative autoinducer-2 (AI-2) aldolase
Alias: ECK1510, JW1510, yneB
Species: Escherichia coli str. K-12 substr. MG1655
Taga M, Semmelhack J, Bassler B. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium. Mol Microbiol. 2001;42:777-93 pubmed
..It is intriguing that an operon regulated by AI-2 encodes functions resembling the ribose transporter, given recent findings that AI-2 is derived from the ribosyl moiety of S-ribosylhomocysteine...
Taga M, Miller S, Bassler B. Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium. Mol Microbiol. 2003;50:1411-27 pubmed
..We also show that two previously uncharacterized members of the lsr operon, LsrF and LsrG, are necessary for the further processing of phospho-AI-2...
Xavier K, Bassler B. Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli. J Bacteriol. 2005;187:238-48 pubmed
..These findings show that, depending on the prevailing growth conditions, the amount of time that the AI-2 signal is present and, in turn, the time that a given community of bacteria remains exposed to this signal can vary greatly. ..
Xavier K, Miller S, Lu W, Kim J, Rabinowitz J, Pelczer I, et al
. Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria. ACS Chem Biol. 2007;2:128-36 pubmed
..This series of chemical events is proposed to enable enteric bacteria to respond to the presence of competitor bacteria by sequestering and destroying AI-2, thereby eliminating the competitors' intercellular communication capabilities. ..
Wang L, Hashimoto Y, Tsao C, Valdes J, Bentley W. Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli. J Bacteriol. 2005;187:2066-76 pubmed
..coli. The elucidation of cAMP-CRP involvement in E. coli autoinduction impacts many areas, including the growth of E. coli in fermentation processes. ..
Wang L, Li J, March J, Valdes J, Bentley W. luxS-dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling. J Bacteriol. 2005;187:8350-60 pubmed
..These data are consistent with the function of LuxS as an important metabolic enzyme but appear not to support the role of AI-2 as a true signal molecule for E. coli W3110 under the investigated conditions. ..
Diaz Z, Xavier K, Miller S. The crystal structure of the Escherichia coli autoinducer-2 processing protein LsrF. PLoS ONE. 2009;4:e6820 pubmed publisher
..One of the Lsr proteins, LsrF, has been implicated in processing the phosphorylated form of AI-2...
Marques J, Oh I, Ly D, Lamosa P, Ventura M, Miller S, et al
. LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2. Proc Natl Acad Sci U S A. 2014;111:14235-40 pubmed publisher
..Here we determine the metabolic fate of intracellular AI-2 by characterizing LsrF, the terminal protein in the Lsr AI-2 processing pathway...