lon

Summary

Gene Symbol: lon
Description: DNA-binding ATP-dependent protease La
Alias: ECK0433, JW0429, capR, deg, dir, lonA, lopA, muc
Species: Escherichia coli str. K-12 substr. MG1655
Products:     lon

Top Publications

  1. Rotanova T, Melnikov E, Khalatova A, Makhovskaya O, Botos I, Wlodawer A, et al. Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains. Eur J Biochem. 2004;271:4865-71 pubmed
    ..Furthermore, this extensive sequence analysis allowed the definition of two subfamilies of Lon proteases, LonA and LonB, based on the consensus sequences in the active sites of their proteolytic domains...
  2. Tomoyasu T, Mogk A, Langen H, Goloubinoff P, Bukau B. Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol. Mol Microbiol. 2001;40:397-413 pubmed
    ..b>Lon was the most efficient protease in degrading misfolded proteins in DnaK-depleted cells...
  3. Mikita N, Cheng I, Fishovitz J, Huang J, Lee I. Processive degradation of unstructured protein by Escherichia coli Lon occurs via the slow, sequential delivery of multiple scissile sites followed by rapid and synchronized peptide bond cleavage events. Biochemistry. 2013;52:5629-44 pubmed publisher
    ..This study utilized a physiological substrate of Escherichia coli Lon protease known as the lambda N protein (?N) to initiate the first kinetic analysis of the proteolytic mechanism of ..
  4. Schoemaker J, Markovitz A. Identification of the gene lon (capR) product as a 94-kilodalton polypeptide by cloning and deletion analysis. J Bacteriol. 1981;147:46-56 pubmed
    A mutation in the lon (capR) gene of Escherichia coli K-12 effects several phenotypic alterations in the mutant cell, such as overproduction of capsular polysaccharide and sensitivity to ultraviolet or ionizing radiation...
  5. Maurizi M. Degradation in vitro of bacteriophage lambda N protein by Lon protease from Escherichia coli. J Biol Chem. 1987;262:2696-703 pubmed
    b>Lon protease from Escherichia coli degraded lambda N protein in a reaction mixture consisting of the two homogeneous proteins, ATP, and MgCl2 in 50 mM Tris, Ph 8.0...
  6. Menon A, Goldberg A. Binding of nucleotides to the ATP-dependent protease La from Escherichia coli. J Biol Chem. 1987;262:14921-8 pubmed
    A critical enzyme in protein breakdown in Escherichia coli is the ATP-hydrolyzing protease La, the lon gene product...
  7. Charette M, Henderson G, Markovitz A. ATP hydrolysis-dependent protease activity of the lon (capR) protein of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1981;78:4728-32 pubmed
    Mutations in the lon (capR) gene result in multiple phenotypes, one of which is the failure to degrade abnormal and normal proteins (Deg-)...
  8. Donch J, Greenberg J. Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Mol Gen Genet. 1968;103:105-15 pubmed
  9. Rosen R, Biran D, Gur E, Becher D, Hecker M, Ron E. Protein aggregation in Escherichia coli: role of proteases. FEMS Microbiol Lett. 2002;207:9-12 pubmed
    ..Aggregates obtained from mutants in the Lon (La) protease contain three times more protein than wild-type aggregates and show the broadest protein diversity...

More Information

Publications95

  1. Griffith K, Shah I, Wolf R. Proteolytic degradation of Escherichia coli transcription activators SoxS and MarA as the mechanism for reversing the induction of the superoxide (SoxRS) and multiple antibiotic resistance (Mar) regulons. Mol Microbiol. 2004;51:1801-16 pubmed
    ..Among single protease mutations, only lon mutations increased the half-life of SoxS and MarA...
  2. Vineyard D, Patterson Ward J, Berdis A, Lee I. Monitoring the timing of ATP hydrolysis with activation of peptide cleavage in Escherichia coli Lon by transient kinetics. Biochemistry. 2005;44:1671-82 pubmed
    Escherichia coli Lon, also known as protease La, is an oligomeric ATP-dependent protease, which functions to degrade damaged and certain short-lived regulatory proteins in the cell. To investigate the kinetic mechanism of E...
  3. Maurizi M, Trisler P, Gottesman S. Insertional mutagenesis of the lon gene in Escherichia coli: lon is dispensable. J Bacteriol. 1985;164:1124-35 pubmed
    The lon gene of Escherichia coli codes for an ATP-dependent protease...
  4. Park S, Jia B, Yang J, Van D, Shao Y, Han S, et al. Oligomeric structure of the ATP-dependent protease La (Lon) of Escherichia coli. Mol Cells. 2006;21:129-34 pubmed
    b>Lon, also known as protease La, belongs to a class of ATP-dependent serine protease...
  5. Nakanishi S, Adhya S, Gottesman M, Pastan I. Effects of dimethylsulfoxide on the E. coli gal operon and on bacteriophage lambda in vivo. Cell. 1974;3:39-46 pubmed
  6. Goldberg A, Waxman L. The role of ATP hydrolysis in the breakdown of proteins and peptides by protease La from Escherichia coli. J Biol Chem. 1985;260:12029-34 pubmed
    The energy requirement for protein breakdown in Escherichia coli appears to be due to protease La, the lon gene product, which hydrolyzes proteins and ATP in a coupled process...
  7. Lamrani S, Ranquet C, Gama M, Nakai H, Shapiro J, Toussaint A, et al. Starvation-induced Mucts62-mediated coding sequence fusion: a role for ClpXP, Lon, RpoS and Crp. Mol Microbiol. 1999;32:327-43 pubmed
    ..It depended upon the host ClpXP and Lon ATP-dependent proteases and the RpoS stationary phase-specific sigma factor, but not upon Crp...
  8. Mizrahi I, Biran D, Ron E. Requirement for the acetyl phosphate pathway in Escherichia coli ATP-dependent proteolysis. Mol Microbiol. 2006;62:201-11 pubmed
    ..In this communication we present evidence for the general role of the acetyl phosphate pathway in protein degradation. ..
  9. Spiridonova V, Kudzhaev A, Melnichuk A, Gainutdinov A, Andrianova A, Rotanova T. [Interaction of DNA Aptamers with the ATP-Dependent Lon Protease from Escherichia coli]. Bioorg Khim. 2015;41:696-700 pubmed
    ATP-dependent Lon protease of E. coli (Ec-Lon) is a key enzyme of the quality control system of the cell proteome...
  10. Ishii Y, Amano F. Regulation of SulA cleavage by Lon protease by the C-terminal amino acid of SulA, histidine. Biochem J. 2001;358:473-80 pubmed
    SulA protein, a cell division inhibitor in Escherichia coli, is degraded by Lon protease...
  11. Calmann M, Marinus M. Regulated expression of the Escherichia coli dam gene. J Bacteriol. 2003;185:5012-4 pubmed
    ..Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
  12. Ebel W, Skinner M, Dierksen K, Scott J, Trempy J. A conserved domain in Escherichia coli Lon protease is involved in substrate discriminator activity. J Bacteriol. 1999;181:2236-43 pubmed
    b>Lon protease of Escherichia coli regulates a diverse set of physiological responses including cell division, capsule production, plasmid stability, and phage replication...
  13. Smith A, Rawlings D. Efficiency of the pTF-FC2 pas poison-antidote stability system in Escherichia coli is affected by the host strain, and antidote degradation requires the lon protease. J Bacteriol. 1998;180:5458-62 pubmed
    ..A decrease in host cell death following plasmid loss from an E. coli lon mutant and a decrease in plasmid stability suggested that the Lon protease plays a role in the rate of turnover of ..
  14. Markovitz A, Rosenbaum N. A regulator gene that is dominant on an episome and recessive on a chromosome. Proc Natl Acad Sci U S A. 1965;54:1084-91 pubmed
  15. Nishii W, Kukimoto Niino M, Terada T, Shirouzu M, Muramatsu T, Kojima M, et al. A redox switch shapes the Lon protease exit pore to facultatively regulate proteolysis. Nat Chem Biol. 2015;11:46-51 pubmed publisher
    The Lon AAA+ protease degrades damaged or misfolded proteins in its intramolecular chamber. Its activity must be precisely controlled, but the mechanism by which Lon is regulated in response to different environments is not known...
  16. Rasulova F, Dergousova N, Starkova N, Melnikov E, Rumsh L, Ginodman L, et al. The isolated proteolytic domain of Escherichia coli ATP-dependent protease Lon exhibits the peptidase activity. FEBS Lett. 1998;432:179-81 pubmed
    ..Here, we obtained the proteolytic domain of Escherichia coli protease Lon by cloning the corresponding fragment of the lon gene in pGEX-KG, expression of the hybrid protein, and isolation ..
  17. Xu L, Link A. Stress responses to heterologous membrane protein expression in Escherichia coli. Biotechnol Lett. 2009;31:1775-82 pubmed publisher
    ..Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein...
  18. Christensen S, Mikkelsen M, Pedersen K, Gerdes K. RelE, a global inhibitor of translation, is activated during nutritional stress. Proc Natl Acad Sci U S A. 2001;98:14328-33 pubmed
    ..of transcription occurred independently of relA and spoT (encoding ppGpp synthetase II), but instead depended on Lon protease. Consistently, Lon was required for degradation of RelB...
  19. Shah I, Wolf R. Sequence requirements for Lon-dependent degradation of the Escherichia coli transcription activator SoxS: identification of the SoxS residues critical to proteolysis and specific inhibition of in vitro degradation by a peptide comprised of the N-term. J Mol Biol. 2006;357:718-31 pubmed
    ..process wherein SoxS synthesis ceases and the intrinsically unstable SoxS protein is rapidly degraded, primarily by Lon protease...
  20. Hansen J, Gregersen N, Bross P. Differential degradation of variant medium-chain acyl-CoA dehydrogenase by the protein quality control proteases Lon and ClpXP. Biochem Biophys Res Commun. 2005;333:1160-70 pubmed
    ..We have studied the role of Lon and ClpXP proteases in handling of wild-type and a folding-impaired disease-associated variant (R28C) of the ..
  21. Shah I, Wolf R. Inhibition of Lon-dependent degradation of the Escherichia coli transcription activator SoxS by interaction with 'soxbox' DNA or RNA polymerase. Mol Microbiol. 2006;60:199-208 pubmed
    ..as if 'soxbox' DNA and RNA polymerase serve as stabilizing ligands in vivo that protect SoxS from degradation by Lon, the protease shown previously to be primarily responsible for its turnover...
  22. Barakat S, Pearce D, Sherman F, Rapp W. Maize contains a Lon protease gene that can partially complement a yeast pim1-deletion mutant. Plant Mol Biol. 1998;37:141-54 pubmed
    We have identified a gene in maize that encodes a product belonging to the Lon protease family. In yeast and mammals, Lon-type proteases catalyze the ATP-dependent degradation of mitochondrial matrix proteins...
  23. Shapira R, Mimran E. Isolation and characterization of Escherichia coli mutants exhibiting altered response to thymol. Microb Drug Resist. 2007;13:157-65 pubmed
    ..in genes whose products are involved in the degradation of short-lived regulatory and abnormal proteins (the lon gene), menaquinone biosynthesis (menA), an unknown function of a putative membrane protein (yagF), synthesis of a ..
  24. Meenakshi S, Munavar M. Suppression of capsule expression in Δlon strains of Escherichia coli by two novel rpoB mutations in concert with HNS: possible role for DNA bending at rcsA promoter. Microbiologyopen. 2015;4:712-29 pubmed publisher
    ..b>Lon protease of Escherichia coli is implicated in the turnover of RcsA (positive regulator of genes involved in ..
  25. Laskowska E, Kuczynska Wisnik D, Skorko Glonek J, Taylor A. Degradation by proteases Lon, Clp and HtrA, of Escherichia coli proteins aggregated in vivo by heat shock; HtrA protease action in vivo and in vitro. Mol Microbiol. 1996;22:555-71 pubmed
    ..Comparison of the rise and decay of the S fraction in mutants defective for heat-shock proteases Lon (La), Clp, HtrA (DegP, Do) and in wild-type strains made studies of proteolysis and the function of the heat-shock ..
  26. Thomas C, Modha J, Razzaq T, Cullis P, Rivett A. Controlled high-level expression of the lon gene of Escherichia coli allows overproduction of Lon protease. Gene. 1993;136:237-42 pubmed
    b>Lon protease from Escherichia coli is an ATP-dependent protease which plays important roles in regulating the levels of specific proteins and in eliminating abnormal proteins...
  27. Nakanishi S, Adhya S, Gottesman M, Pastan I. In vitro repression of the transcription of gas operon by purified gal repressor. Proc Natl Acad Sci U S A. 1973;70:334-8 pubmed
    ..We conclude that the gal repressor recognizes the gal operator site and acts by preventing gal transcription. ..
  28. Fischer H, Glockshuber R. A point mutation within the ATP-binding site inactivates both catalytic functions of the ATP-dependent protease La (Lon) from Escherichia coli. FEBS Lett. 1994;356:101-3 pubmed
    ..Since selective inactivation of the peptidase activity of La does not affect its intrinsic ATPase activity, coupling of proteolysis with ATP hydrolysis is only uni-directional in this energy-dependent protease. ..
  29. Van Melderen L, Bernard P, Couturier M. Lon-dependent proteolysis of CcdA is the key control for activation of CcdB in plasmid-free segregant bacteria. Mol Microbiol. 1994;11:1151-7 pubmed
    ..In this paper, we show that the CcdA protein is unstable and that the degradation of CcdA is dependent on the Lon protease...
  30. Wang L, Elliott M, Elliott T. Conditional stability of the HemA protein (glutamyl-tRNA reductase) regulates heme biosynthesis in Salmonella typhimurium. J Bacteriol. 1999;181:1211-9 pubmed
    ..This was confirmed by the finding that HemA turnover is completely blocked in a lon clpP double mutant of E. coli. Each single mutant shows only a small effect...
  31. Charette M, Henderson G, Doane L, Markovitz A. DNA-stimulated ATPase activity on the lon (CapR) protein. J Bacteriol. 1984;158:195-201 pubmed
    The gene product of the pleiotropic lon (also called capR) locus in Escherichia coli, the CapR protein, is an ATP hydrolysis-dependent protease and a nonspecific nucleic acid-binding protein...
  32. Langklotz S, Narberhaus F. The Escherichia coli replication inhibitor CspD is subject to growth-regulated degradation by the Lon protease. Mol Microbiol. 2011;80:1313-25 pubmed publisher
    ..The Escherichia coli Lon protease is involved in the control of the SOS response, acid tolerance and nutritional deprivation...
  33. Nishii W, Maruyama T, Matsuoka R, Muramatsu T, Takahashi K. The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli. Eur J Biochem. 2002;269:451-7 pubmed
    SulA protein is known to be one of the physiological substrates of Lon protease, an ATP-dependent protease from Escherichia coli. In this study, we investigated the cleavage specificity of Lon protease toward SulA protein...
  34. Waxman L, Goldberg A. Protease La from Escherichia coli hydrolyzes ATP and proteins in a linked fashion. Proc Natl Acad Sci U S A. 1982;79:4883-7 pubmed
    ..breakdown in Escherichia coli results from an ATP requirement for the function of protease La, the product of the lon gene. This novel serine protease contains an ATPase activity that is essential for proteolysis...
  35. Vineyard D, Patterson Ward J, Lee I. Single-turnover kinetic experiments confirm the existence of high- and low-affinity ATPase sites in Escherichia coli Lon protease. Biochemistry. 2006;45:4602-10 pubmed
    b>Lon is an ATP-dependent serine protease that degrades damaged and certain regulatory proteins in vivo...
  36. Ranquet C, Gottesman S. Translational regulation of the Escherichia coli stress factor RpoS: a role for SsrA and Lon. J Bacteriol. 2007;189:4872-9 pubmed
    ..We report here that SsrA is needed for the correct high-level translation of RpoS. The ATP-dependent protease Lon was also found to negatively affect RpoS translation, but only at low temperature...
  37. Kuroda A. A polyphosphate-lon protease complex in the adaptation of Escherichia coli to amino acid starvation. Biosci Biotechnol Biochem. 2006;70:325-31 pubmed
    ..PolyP forms a complex with the ATP-dependent Lon protease. The formation of a complex then enables Lon to degrade free ribosomal proteins...
  38. Van Melderen L, Thi M, Lecchi P, Gottesman S, Couturier M, Maurizi M. ATP-dependent degradation of CcdA by Lon protease. Effects of secondary structure and heterologous subunit interactions. J Biol Chem. 1996;271:27730-8 pubmed
    ..protein of the ccd post-segregational killing system carried by the F plasmid, was degraded in vitro by purified Lon protease from Escherichia coli...
  39. Melnikov E, Andrianova A, Morozkin A, Stepnov A, Makhovskaya O, Botos I, et al. Limited proteolysis of E. coli ATP-dependent protease Lon - a unified view of the subunit architecture and characterization of isolated enzyme fragments. Acta Biochim Pol. 2008;55:281-96 pubmed
    We carried out chymotryptic digestion of multimeric ATP-dependent Lon protease from Escherichia coli...
  40. Vineyard D, Zhang X, Lee I. Transient kinetic experiments demonstrate the existence of a unique catalytic enzyme form in the peptide-stimulated ATPase mechanism of Escherichia coli Lon protease. Biochemistry. 2006;45:11432-43 pubmed
    b>Lon is an oligomeric serine protease whose proteolytic activity is mediated by ATP hydrolysis...
  41. Johansson J, Uhlin B. Differential protease-mediated turnover of H-NS and StpA revealed by a mutation altering protein stability and stationary-phase survival of Escherichia coli. Proc Natl Acad Sci U S A. 1999;96:10776-81 pubmed
    ..Using protease-deficient mutants, we obtained evidence that the Lon protease was responsible for the degradation of StpA...
  42. Zehnbauer B, Markovitz A. Cloning of gene lon (capR) of Escherichia coli K-12 and identification of polypeptides specified by the cloned deoxyribonucleic acid fragment. J Bacteriol. 1980;143:852-63 pubmed
    A mutation in the lon (capR) gene of Escherichia coli K-12 results in overproduction of capsular polysaccharide and increased sensitivity to ultraviolet and ionizing radiations...
  43. Oh J, Eun Y, Yoo S, Seol J, Seong I, Lee C, et al. LonR9 carrying a single Glu614 to Lys mutation inhibits the ATP-dependent protease La (Lon) by forming mixed oligomeric complexes. Biochem Biophys Res Commun. 1998;250:32-5 pubmed
    An unusual lon mutation (called lonR9) is dominant over the wild-type gene, which encodes the ATP-dependent protease La (Lon) in Escherichia coli, when present in multicopy plasmids...
  44. Malik M, Capecci J, Drlica K. Lon protease is essential for paradoxical survival of Escherichia coli exposed to high concentrations of quinolone. Antimicrob Agents Chemother. 2009;53:3103-5 pubmed publisher
    A deficiency of the Escherichia coli Lon protease blocked paradoxical survival occurring at very high nalidixic acid concentrations. The absence of Lon also blocked a parallel increase in cell lysate viscosity likely to reflect DNA size...
  45. Christensen S, Maenhaut Michel G, Mine N, Gottesman S, Gerdes K, Van Melderen L. Overproduction of the Lon protease triggers inhibition of translation in Escherichia coli: involvement of the yefM-yoeB toxin-antitoxin system. Mol Microbiol. 2004;51:1705-17 pubmed
    In Escherichia coli, the Lon ATP-dependent protease is responsible for degradation of several regulatory proteins and for the elimination of abnormal proteins. Previous studies have shown that the overproduction of Lon is lethal...
  46. García Fruitós E, Martínez Alonso M, Gonzalez Montalban N, Valli M, Mattanovich D, Villaverde A. Divergent genetic control of protein solubility and conformational quality in Escherichia coli. J Mol Biol. 2007;374:195-205 pubmed
    ..genetic deficiencies in different cytosolic chaperones and proteases (affecting DnaK, GroEL, GroES, ClpB, ClpP and Lon at different extents) resulted in much less soluble but unexpectedly more fluorescent polypeptides...
  47. Kudzhaev A, Andrianova A, Serova O, Arkhipova V, Dubovtseva E, Rotanova T. [The Effect of Mutations in the Inserted Domain of ATP-Dependent Lon Protease from E. coli on the Enzyme Function]. Bioorg Khim. 2015;41:579-86 pubmed
    ATP-Dependent protease LonA from E. coli (Ec-Lon), belonging to the superfamily of AAA+ proteins, is a key member of the protein quality control system in bacterial cells...
  48. Pruteanu M, Baker T. Proteolysis in the SOS response and metal homeostasis in Escherichia coli. Res Microbiol. 2009;160:677-83 pubmed publisher
  49. SaiSree L, Reddy M, Gowrishankar J. lon incompatibility associated with mutations causing SOS induction: null uvrD alleles induce an SOS response in Escherichia coli. J Bacteriol. 2000;182:3151-7 pubmed
    ..We have shown in this study that null mutations in uvrD are incompatible with lon, the incompatibility being a consequence of the chronic induction of SOS in uvrD strains and the resultant ..
  50. Andrianova A, Kudzhaev A, Serova O, Dergousova N, Rotanova T. [Role of the α-helical domains in the functioning of ATP-dependent Lon protease of Escherichia coli]. Bioorg Khim. 2014;40:673-81 pubmed
    Homooligomeric ATP-dependent LonA proteases are bifunctional enzymes belonging to the superfamily of AAA+ proteins...
  51. Yamaguchi Y, Tomoyasu T, Takaya A, Morioka M, Yamamoto T. Effects of disruption of heat shock genes on susceptibility of Escherichia coli to fluoroquinolones. BMC Microbiol. 2003;3:16 pubmed
    ..Mutations in dnaK, groEL, and lon increased this susceptibility; the lon mutant exhibited the greatest effects...
  52. Menon A, Waxman L, Goldberg A. The energy utilized in protein breakdown by the ATP-dependent protease (La) from Escherichia coli. J Biol Chem. 1987;262:722-6 pubmed
    ..the pathway for protein degradation in Escherichia coli is protease La, an ATP-hydrolyzing protease encoded by the lon gene. This enzyme degrades various proteins to small polypeptides containing 10-20 amino acid residues...
  53. Higashitani A, Ishii Y, Kato Y, Koriuchi K. Functional dissection of a cell-division inhibitor, SulA, of Escherichia coli and its negative regulation by Lon. Mol Gen Genet. 1997;254:351-7 pubmed
    ..C-terminal deletions of 8 and 21 residues increased the growth-inhibiting activity in lon+ cells, but not in lon- cells. The wild-type and mutant SulA proteins were isolated in a form fused to E...
  54. Dopazo A, Tormo A, Aldea M, Vicente M. Structural inhibition and reactivation of Escherichia coli septation by elements of the SOS and TER pathways. J Bacteriol. 1987;169:1772-6 pubmed
    ..FtsA3, could be reactivated one doubling time after removal of the inhibitory agent in the presence of an active lon gene product...
  55. Ignatov K, Chistiakova L. [Integration of IS186 element into the -10-region of the promoter of heat shock lon-gene in Escherichia coli]. Bioorg Khim. 1997;23:18-20 pubmed
    The insertion element IS186 was found to be incorporated into the -10 region of the promoter of the E. coli lon gene. The integration represses lon gene expression. Homology between the integration sites of IS186, the -10 region of E...
  56. Chung C, Goldberg A. DNA stimulates ATP-dependent proteolysis and protein-dependent ATPase activity of protease La from Escherichia coli. Proc Natl Acad Sci U S A. 1982;79:795-9 pubmed
    The product of the lon gene in Escherichia coli is an ATP-dependent protease, protease La, that also binds strongly to DNA...
  57. Lee I, Berdis A, Suzuki C. Recent developments in the mechanistic enzymology of the ATP-dependent Lon protease from Escherichia coli: highlights from kinetic studies. Mol Biosyst. 2006;2:477-83 pubmed
    b>Lon protease, also known as protease La, is one of the simplest ATP-dependent proteases that plays vital roles in maintaining cellular functions by selectively eliminating misfolded, damaged and certain short-lived regulatory proteins...
  58. Nazir A, Harinarayanan R. Inactivation of Cell Division Protein FtsZ by SulA Makes Lon Indispensable for the Viability of a ppGpp0 Strain of Escherichia coli. J Bacteriol. 2015;198:688-700 pubmed publisher
    ..In this study, we show that the ppGpp(0) strain, unlike the wild type, requires the Lon protease for cell division and viability in LB...
  59. Luo S, McNeill M, Myers T, Hohman R, Levine R. Lon protease promotes survival of Escherichia coli during anaerobic glucose starvation. Arch Microbiol. 2008;189:181-5 pubmed
    In Escherichia coli, Lon is an ATP-dependent protease which degrades misfolded proteins and certain rapidly-degraded regulatory proteins...
  60. Van Melderen L, Aertsen A. Regulation and quality control by Lon-dependent proteolysis. Res Microbiol. 2009;160:645-51 pubmed publisher
    After their first discovery in Escherichia coli, Lon homologues were found to be widely distributed among prokaryotes to eukaryotes...
  61. Botos I, Melnikov E, Cherry S, Khalatova A, Rasulova F, Tropea J, et al. Crystal structure of the AAA+ alpha domain of E. coli Lon protease at 1.9A resolution. J Struct Biol. 2004;146:113-22 pubmed
    ..of the small, mostly helical alpha domain of the AAA+ module of the Escherichia coli ATP-dependent protease Lon has been solved by single isomorphous replacement combined with anomalous scattering and refined at 1...
  62. Biran D, Gur E, Gollan L, Ron E. Control of methionine biosynthesis in Escherichia coli by proteolysis. Mol Microbiol. 2000;37:1436-43 pubmed
    ..The enzyme is stable in triple mutants defective in Lon-, HslVU- and ClpP-dependent proteases...
  63. Chin D, Goff S, Webster T, Smith T, Goldberg A. Sequence of the lon gene in Escherichia coli. A heat-shock gene which encodes the ATP-dependent protease La. J Biol Chem. 1988;263:11718-28 pubmed
    To learn more about the mechanism and regulation of the ATP-dependent protease La in Escherichia coli, the lon gene was completely sequenced using the dideoxy method on fragments generated by Bal31 digestion...
  64. Waxman L, Goldberg A. Protease La, the lon gene product, cleaves specific fluorogenic peptides in an ATP-dependent reaction. J Biol Chem. 1985;260:12022-8 pubmed
    ..0-9.5, 5) the protein purified from lon- E...
  65. SaiSree L, Reddy M, Gowrishankar J. IS186 insertion at a hot spot in the lon promoter as a basis for lon protease deficiency of Escherichia coli B: identification of a consensus target sequence for IS186 transposition. J Bacteriol. 2001;183:6943-6 pubmed
    ..described more than 50 years ago, and the genetic locus responsible for the trait was subsequently identified as lon (encoding Lon protease). We now show that both E. coli B and the first reported E...
  66. Pohl A, Bugajer Gleitman H, Lachmann D, Moser K. [Glutathione reductase deficiency with membrane defect in hereditary hemolytic anemia]. Acta Haematol. 1976;56:47-57 pubmed
    ..In all cases splenectomy beneficially influenced the hemolytic process. Family studies revealed a dominant-autosomal genetic transmission. ..
  67. Nicoloff H, Perreten V, McMurry L, Levy S. Role for tandem duplication and lon protease in AcrAB-TolC- dependent multiple antibiotic resistance (Mar) in an Escherichia coli mutant without mutations in marRAB or acrRAB. J Bacteriol. 2006;188:4413-23 pubmed
    ..Two mutations were found: an insertion in the promoter of lon (lon3::IS186) that occurred first and a subsequent large tandem duplication, dupIS186, bearing the genes acrAB and ..
  68. Choy J, Aung L, Karzai A. Lon protease degrades transfer-messenger RNA-tagged proteins. J Bacteriol. 2007;189:6564-71 pubmed
    ..We present in vivo and in vitro evidence that demonstrates a direct role for the Lon ATP-dependent protease in the degradation of tmRNA-tagged proteins...
  69. Mel nikov E, Tsirul nikov K, Rotanova T. [Coupling of proteolysis with ATP hydrolysis by Escherichia coli Lon proteinase. I. Kinetic aspects of ATP hydrolysis]. Bioorg Khim. 2000;26:530-8 pubmed
    Some aspects of the ATPase function of the Escherichia coli Lon protease were studied around the optimum pH value...
  70. Slominska M, Wahl A, Wegrzyn G, Skarstad K. Degradation of mutant initiator protein DnaA204 by proteases ClpP, ClpQ and Lon is prevented when DNA is SeqA-free. Biochem J. 2003;370:867-71 pubmed
    ..Its cognate protease ClpQ, as well as Lon protease, degraded DnaA204 to the same degree as ClpP...
  71. Duval V, Nicoloff H, Levy S. Combined inactivation of lon and ycgE decreases multidrug susceptibility by reducing the amount of OmpF porin in Escherichia coli. Antimicrob Agents Chemother. 2009;53:4944-8 pubmed publisher
    ..encoding a putative transcriptional regulator, led to decreased multidrug susceptibility in an Escherichia coli lon mutant. The multidrug susceptibility phenotype (e.g...
  72. Sherman MYu -, Goldberg A. Involvement of the chaperonin dnaK in the rapid degradation of a mutant protein in Escherichia coli. EMBO J. 1992;11:71-7 pubmed
    ..degradation of phoA61 at 37 degrees C and 41 degrees C is in part by protease La, since it decreased by 50% in lon mutants...
  73. Fu G, Smith M, Markovitz D. Bacterial protease Lon is a site-specific DNA-binding protein. J Biol Chem. 1997;272:534-8 pubmed
    The product of the Escherichia coli lon gene is the ATP-dependent Lon protease...
  74. Zehnbauer B, Foley E, Henderson G, Markovitz A. Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein. Proc Natl Acad Sci U S A. 1981;78:2043-7 pubmed
    The polypeptide product of the lon (capR) gene was identified and partially purified from bacterial strains homozygous for the capR(+) or capR9 (ochre mutation) alleles cloned with pSC101...
  75. Wetzstein M, Schumann W. Promoters of major Escherichia coli heat shock genes seem non-functional in Bacillus subtilis. FEMS Microbiol Lett. 1990;60:55-8 pubmed
    ..in Bacillus subtilis, the regulatory regions including the heat shock promoters of the main heat shock genes dnaKJ, lon, groES, and htpG were fused to the indicator genes lacZ and cat...
  76. Bissonnette S, Rivera Rivera I, Sauer R, Baker T. The IbpA and IbpB small heat-shock proteins are substrates of the AAA+ Lon protease. Mol Microbiol. 2010;75:1539-49 pubmed publisher
    ..We report that IbpA and IbpB, the sHSPs of Escherichia coli, are substrates for the AAA+ Lon protease...
  77. Gill R, Valdes J, Bentley W. A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli. Metab Eng. 2000;2:178-89 pubmed
    ..Specifically, the responses included significantly increased upregulation of heat shock (ftsH, clpP, lon, ompT, degP, groEL, aceA, ibpA), SOS/DNA damage (recA, lon, IS5 transposase), stationary phase (rpoS, aceA), and ..
  78. Sundermeier T, Ge Z, Richards J, Dulebohn D, Karzai A. Studying tmRNA-mediated surveillance and nonstop mRNA decay. Methods Enzymol. 2008;447:329-58 pubmed publisher
    ..Several proteases (ClpXP, ClpAP, Lon, FtsH, and Tsp) are known to be involved in the degradation of tmRNA-tagged proteins (Choy et al...
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    ATP-dependent Lon protease degrades specific short-lived regulatory proteins as well as defective and abnormal proteins in the cell...
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    We report here the first crystal structure of the N-terminal domain of an A-type Lon protease...
  81. Gur E, Vishkautzan M, Sauer R. Protein unfolding and degradation by the AAA+ Lon protease. Protein Sci. 2012;21:268-78 pubmed publisher
    ..Here, we explore the ability of the E. coli Lon protease to unfold and degrade model protein substrates beginning at N-terminal, C-terminal, or internal degrons...
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    Mutations in sulA (sfiA) block the filamentation and death of capR (lon) mutants that occur after treatments that either damage DNA or inhibit DNA replication and thereby induce the SOS response...
  83. Ishii Y, Sonezaki S, Iwasaki Y, Miyata Y, Akita K, Kato Y, et al. Regulatory role of C-terminal residues of SulA in its degradation by Lon protease in Escherichia coli. J Biochem. 2000;127:837-44 pubmed
    The SulA protein is a cell division inhibitor in Escherichia coli, and is specifically degraded by Lon protease...
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    ..Five different lon mutations, not related to the Mar phenotype, were also found in 12 of the 13 mutants...
  85. Leffers G, Gottesman S. Lambda Xis degradation in vivo by Lon and FtsH. J Bacteriol. 1998;180:1573-7 pubmed
    ..The Bacteriophage Lambda, 1971). We found that Xis is degraded in vivo by two ATP-dependent proteases, Lon and FtsH (HflB)...
  86. Bonnefoy E, Almeida A, Rouviere Yaniv J. Lon-dependent regulation of the DNA binding protein HU in Escherichia coli. Proc Natl Acad Sci U S A. 1989;86:7691-5 pubmed
    ..HU1 and HU2 subunits, using pulse-chase labeling experiments, showed that the HU1 subunit is degraded specifically in the absence of the HU2 subunit and, moreover, that this degradation is dependent on the presence of the Lon protease.